HOSTED VIRTUALLY ON FORGERONE APRIL 29-30, 2021 - MCQUEARY ...
←
→
Page content transcription
If your browser does not render page correctly, please read the page content below
Hosted Virtually on
ForgerOne
April 29-30, 2021McQueary College of Health and Human Services
Student Research Symposium
Welcome to the 25th Annual McQueary College of Health and Human Services Student Research Symposium.
The purpose of the symposium is to highlight student research and to provide an opportunity for students to gain
experience discussing their scholarly work.
Above all, the symposium provides the MCHHS community with an opportunity to celebrate students’
outstanding work, to acknowledge the faculty members who serve as mentors, and to recognize the schools and
departments which support discovery. On this 25th anniversary of the symposium, we are excited to welcome
members of the newly constituted MCHHS Advisory Board to this virtual event.
To all McQueary College of Health and Human Services faculty, staff, alumni, board members and students,
THANK YOU for participating in this year’s symposium! Your hard work, dedication, and active participation is
greatly appreciated.
I would also like to thank the MCHHS Student Research Symposium Committee for their time and effort in
organizing the symposium:
Dr. Ashlea Cardin, Occupational Therapy
Dr. Christie Cathey, Psychology
Dr. Melissa Fallone, Psychology
Dr. James Hackney, Physical Therapy
Dr. Michael Hudson, Public Health and Sports Medicine
Dr. Anne Marie Hunter, Biomedical Sciences
Dr. Wafaa Kaf, Communication Sciences and Disorders
Dr. Bogdan Kostic, Psychology
Dr. Jianjie Wang, Biomedical Sciences
Dr. Letitia White-Minnis, Associate Dean
Mr. Ian Alaimo, Technology Coordinator
Mr. Jeremy Henson, Distributed User Support Specialist
Mrs. Rebeca Reut-Robinson, Executive Assistant
Dr. Mark A. Smith
Dean, McQueary College of Health and Human Services
2Presenters and Faculty Mentors by Department and Page Number
Biomedical Sciences
Jackson, Travis………………………….7
Allen, Natalie…………………...........7
James, Brianna…………………...........15
Amuedo, Daniel……………………...9
Jennings, Jessica……………………….14
Armstrong, Kaitlyn…………………..7
Kendrick, Kelsey………………............15
Barousse, Austin.………………….....7
Knipp, Elizabeth……………………….14
Bates, Tyler…………………..............7
Laffoon, Anna…………………………12
Bays, Lauren………………………....2
Linhardt, Jacob………………………….7
Bertoldie, Matthew…………………..3
Macander, Bridgette…………………...15
Brindley, Samantha………………......7
Matheney, Hannah…………………….10
Brodeur, Amanda…………………..7,8
McWoods, Anna………………………..7
Brooks, Patrick……………………….8
Mertins-Chiodini, Ana…………….........8
Casey, Shae…………………………..7
Monje, Emily…………………………..12
Chisum, Emily……………………......9
Morote Galvez, Gianfranco ……….13, 16
Dougless, Keri……………………....12
Murray, Sarah………………………….12
Fields, Ceira…………………………13
Nichols, Claire…………………………15
Fisher, Abigail……………………....13
Orlando, Sofia………………………….15
Fleming, Alyssa……………………..14
Ott, Lucas………………………………11
Fox, Jordan…………………………...8
Piland, Deborah…………………12,13,14
Freese, Matthew………………………7
Poisson, Abbie…………………………..8
Gann, Abby………………………..9,15
Pomeroy, Benjamin……………………..8
Garrad, Richard……………………....9
Rivas, Christian………………………..15
Gherardini, Gina………………….......8
Roberts, Hillary…………………………7
Gibson, Jacob…………………….......8
Robertson, Meghann…………………...14
Gieselmann, Alyssa…………………..7
Roy, Tharron…………………………….9
Graham, Emily………………………10
Rudolph, Emily………………………...13
Gruener, Shane………………………16
Sadaro, Sophia…………………………..7
Hatten, Jeremy…………………….....12
Sands, Taylor…………………………..12
Hough, Lyon…………………………10
Sappington, Gabriela…………………..16
Hulme, Amy………………….......10,11
Sesti, Emma……………………………14
Hunter, Anne Marie…………………11
Shupp, Trey……………………………10
Ingram, Rachael………………….......7
Sams, Austin…………………………...16
Isaacks, Olivia………………………..7
3Presenters and Faculty Mentors by Department and Page Number
Smith, Kelsey………………………..12 Holko, Amber…………………………16
Smith, Tayla…………………………13 Karnuth, Emily………………………..16
Tebbe, Travis………………………..15 Kelker, Danielle……………………….17
Tisch, Hadyn…………………………9 Koch, Brea…………………………….22
Tsai, Henry…………………………..14 Lockenvitz, Sarah………………….19,20
Ulbricht, Randi………………………15 Lyon, Keelyn………………………….22
Urich, Stephanie……………………..13 Mak, Ka……………………………….17
Van Biljon, Lillian……………………7 Mantie-Kozlowski, Alana………20,21,22
Walker, Taylor………………………16 McClernon, Carly……………………..22
Wang, Jianjie………………………...15 Mills, Marissa…………………………20
Weber, Kaitlyn………………………..7 Petty, Brendynn………………………..21
Williams, Audrey…………………….14 Reeves, Emma………………………....21
Wilson, Lindsey……………………...14 Robinson, Susan………….....................23
Wise, Emma………………………….11 Rogers, Jordan………………………...19
Witkowski, Colette…………………..16 Roy, Lesa……………………………...23
Wombacher, Petyton………………….7 Rushing, Madison……………………..20
Yates, Hannah……….……………….11 Steppig, Emily………………………...18
Zelle, Leanna……………………….9,15 Sudduth, Samantha……………………23
Tennyson, Samantha…………………..18
Communication Sciences and Disorders Thomas-Tate, Shurita………………….22
Bahl, Alex……………………………20 Turntine, Macie………………………..22
Ballay, Monica……………………….22 Ulrich, Macayah………………………18
Barber, Sarah…………………………23 Wegrzyn, Tyler………………………..19
Braden, Hannah………………………19 White-Minnis, Letitia………………….23
Buchanan, Tiffany……………………19 Zitting, Amanda……………………….19
Carron, Kelci…………………………20
Combs, Callie………………………...20 Kinesiology
Doza, Ashton…………………………23 Bushman, Barbara……………………..23
Fielder, Joni…………………………..19 Goddard, Stacy………………………...23
Fong, Aki……………………………..21 Klabunde, Ryanne…….……………….23
Grills, Hannah………………………..22 Powell, Sara…………………………....23
Hiebert, Lindsey……………16,17,18,19 Scace, Delaney………………………...23
4Presenters and Faculty Mentors by Department and Page Number
Nursing
Salmon, Ashton………………………..25
Chamberlin, Allison………………….24
Shockley, Paige………………………..26
Johnson, Lorilea……………………...24
Slusher, Hannah………………………..25
Kuhn, Alison…………………………24
Steinbach, Madeline…………………...26
Million, Rita………………………….24
Waller, Bailey………………………….25
Penkalski, Melissa……………………24
Willey, Marc…………………………...26
Smith, Diane………………………….24
Willison-Allen, Mayce………………...25
Occupational Therapy
Physical Therapy
Allen, Lauren………………………...26
Braunberger, Mark……………………..27
Amos, Gabrielle……………………...26
Black, Brendan…………………………27
Bellows, Colleen……………………..25
Brown, Bryce ………………………….27
Boehne, Tara…………………………25
Eaves, Hannah…….……………………27
Cardin, Ashlea………………………..25
Hackney, James………………………...27
Chakraborty, Sapna…………………..25
Harker, Gerard………………………….27
Ebert, Amanda………………………..25
Holtmeier, Mary………………………..27
Flint, Leo……………………………..26
Potthast, Amy…………………………..27
Garrison, Traci……………………….26
Rhodes, Collin………………………….27
Hansen, Katie………………………...25
Waterway, Tyler………………………..27
Heller, Griffen………………………..25
Wilcoxon, Sarah………………………..27
Hermann, Maria……………………...26
Isbell, Haley………………………….25
Jennings, Chelsea…………………….26 Psychology
Jungbluth, Amy……………………....26 Baron, Olivia…………………………...30
Lacker, Allison……………………….25 Bettis, Bryce……………………………33
Meyers, Mackenzie…………………..25 Bonnot, Amanda………………………..31
McCurry, Kori……………………….26 Cathey, Christie…………………….27, 28
Mullins, Meshila……………………..25 Cooper, Kennedy……………………….31
Nardi, Samantha……………………..26 Crain, Sarah…………………………….28
Perkins, Jessica………………………25 Cummins, Courtney……………………30
Echols, Leslie…………………………..28
5Presenters and Faculty Mentors by Department and Page Number
Public Health and Sports Medicine
Fischer, Donald…………………...29,30
Carr, David…………………………..33,34
Fallone, Melissa…………………..28,29
Christian, McCall………………………..35
Hadley, Jorre……………………...31,33
Freitas, Ethan……………………………34
Harmon, Skyler………………………32
Franko, Molly…………………………...33
Harty, Megan…………………………33
Gullett, Grace……………………………33
Henderson, Jazmyn…………………...31
Harold, Erin……………………………..34
Hoff, Tegan…………………………...31
Hetland-Purna, Ariana…………………..34
Jungmeyer, Savannah………………...28
Hetzler, Tona…………………………34,35
Lischwe, Max………………………...29
Holwerda, Kennedy……………………..34
Massey-Abernathy, Amber………..30,31
Hudson, Michael………………………...35
Needy, Lydia…………………..27,28,30
Kreklow, Allison………………………...35
Ong, Xin Wei………………………...29
Lefresne, Fiona………………………….34
Payne, Ashley………………….31,32,33
Liggett, Allan……………………………35
Phipps, Weston……………………….31
Mikesell, Lexie…………………………..34
Pulse, Hannah………………………...31
Morlan, Caleb…………………………....34
Rucker, Kaylee……………………….32
Noskowiak, Melanie…………………….35
Schaffer, Derrick……………………..29
Osborn, Ayron…………………………...34
Sparks, Emma………………………...28
Primrose, Katie…………………………..34
Stanbrough, CaSandra……………..30,31
Ratsch, Corey……………………………33
Stoll, Nicholas………………………..33
Reed, Hannah……………………………35
Travis, Erinmarie…………………..32,33
Seki, Taisei………………………………35
Troutwine, Elizabeth………………….32
Stennis, Tameka.………………………...35
Vonarx, Mary…………………………32
Teeter, Haili……………………………...34
Wall, Rebekkah……………………….31
Williams, Caitlin………………………...34
Watson, Brooke……………………....30
Yancey, Sydnee………………………….35
Woemmel, Samantha…………………28
Zurheide, Alexis…………………………35
Won, Haein…………………………...28
Yadon, Carly………………………….32
Young-Jones, Adena………………32,33
6Abstracts
BIOMEDICAL SCIENCES iduronidase (IDUA). Hurler syndrome is
characterized by cognitive disabilities, hearing
Reported Food Insecurity and Interventions of impairment, organ enlargement, as well as
Collegiate Athletes at a Public Midwestern degenerative joint disease and bone deformities.
University Despite current treatments to improve the
Shae Casey, Austin Barousse, Natalie Allen, Hillary symptoms, very few improve the bone phenotype.
Roberts As a result, diagnosed individuals experience
Faculty Advisor: Natalie Allen disabling, painful bone disease that often requires
surgical intervention. The Brodeur lab examines the
This study assesses meal funding provided to bone phenotype using a knock-in mouse model,
student athletes, situational impacts, and food containing a nonsense mutation analogous to the
insecurity relief. Research shows athletes and human IDUA mutation. To better understand the
students who are food insecure experience decreased physiochemical composition of the bone content of
athletic and academic performance, and declined the IDUA mouse model, Immunohistochemistry can
mental and physical health (Coleman, 2019). The be used. This method targets antigens in the tissue
purpose of this study was to determine if the student by adding a primary and a secondary antibody to the
athlete population at a public university was at risk bone sample. Immunohistochemistry examining
for food insecurity, identify related factors, facilitate RANK was performed to examine osteoclast
interventions, and to determine the situational function in the mouse model. In this study we
impact of the SARS-CoV-2 (Covid-19) pandemic on demonstrate the ability to decalcify, fix, embed, and
food security. An online survey was distributed to stain murine long bones. Through the procedures
391 athletes at the university and a total of 79 mentioned, the goal of this research is to further
surveys (20%) were returned. Those that reported understand the bone phenotype seen in Hurler
having a meal plan or stipend for meals was 38% of syndrome.
the total participants. Data showed 30% of
participants experienced food insecurity and 11%
were situational due to the Covid-19 pandemic. All Further Investigation of the Initiating
food insecure participants had taken interventions to Mechanism of the Type I Collagen
alleviate their situation, the most popular Glomerulopathy
intervention being to seek help from friends and Matthew Freese, Kaitlyn Armstrong, Rachael
family. Based on outcomes, the team dietitian will Ingram, Travis Jackson, Lillian van Biljon, Kaitlyn
provide information on the university food pantry Weber, Petyton Wombacher, Amanda Brodeur
and emergency scholarships. This study will help Faculty Advisor: Amanda Brodeur
provide a foundation to combat food insecurity."
In chronic renal disease, the progressive
accumulation of collagen and other extracellular
Further Characterization of the Skeletal matrix proteins in the mesangium of nephrons
Phenotype in IDUA-W392X Knock-In Mice as a results in fibrosis, glomerulosclerosis, and eventual
Model of Mucopolysaccharidosis Type 1 renal failure. Mice deficient in proa2(I) collagen are
Samantha Brindley, Sophia Sadaro, Alyssa a model for Osteogenesis Imperfecta and Type 1
Gieselmann, Jacob Linhardt, Olivia Isaacks, Tyler Collagen Glomerulopathy, because homotrimeric
Bates, Anna McWoods, Amanda Brodeur type I collagen is accumulated in the mesangium.
Faculty Advisor: Amanda Brodeur Picrosirius red (PSR) staining was used to evaluate
collagen deposition. Histological evaluation and
Mucopolysaccharidosis type 1 (Hurler syndrome) is lesion scoring of kidney sections demonstrates that
a rare, autosomal recessive disorder caused by the in comparison to wild-type mice, Col1a2-deficient
deficiency in the lysosomal enzyme alpha-L- homozygous and heterozygous mice exhibit
7Abstracts
abnormal glomerular collagen deposition. Following Pediatric-Onset Orthopedic Disorders
staining, immunohistochemistry will be used to Abbie Poisson, Amanda Brodeur
determine if platelet-derived growth factor (PDGF) Faculty Advisor: Amanda Brodeur
B and D are responsible for initiating
glomerulopathy in our mouse models. PDGFs The human skeleton is a specialized organ that
contribute to healing wounds, repairing damage, provides a framework for the human body, supports
building blood vessels, and growth of mesenchymal and protects numerous organs and structures, stores
cells. These are major contributors to fibrosis in and releases minerals, and allows movement. As a
kidneys. Previous research has shown that PDGF are metabolically active organ, bone undergoes constant
commonly involved in fibrosis, especially in modifications to adjust to the body’s physical
kidneys. An association between either PDGF B or stresses and metabolic needs. The production of the
D and homotrimeric collagen could potentially lead major biomolecules and regulation of signal cascade
to ways of limiting renal fibrosis. pathways becomes vital for the proper formation,
modification, and degradation of bone and its
surrounding tissue as children develop. If the
COVID-19 Confusion: Mechanism, Ethics, and metabolism of these biomolecules and tissues
Hesitancy Surrounding the COVID-19 Vaccines becomes dysregulated, various skeletal diseases can
Jacob Gibson, Jordan Fox, Gina Gherardini, Ana evolve and cause a wide range of physical ailments.
Mertins Chiodini, Abbie Poisson Examining biomolecules’ impact on bone health,
Faculty Advisor: Amanda Brodeur innovative research continues to provide insights
into protein, nucleic acid, and carbohydrates’
According to the World Health Organization, relation to hypophosphatasia, cleidocranial
COVID-19, caused by the SARS-CoV-2 virus, has dysplasia, and mucopolysaccharidosis type I.
swept across the globe infecting over 124 million Moreover, researchers are uncovering the
and killing more than 2.7 million people worldwide pathophysiological cause of early-onset
as of mid-March 2021. In December of 2019, the osteoarthritis. This presentation will expound on the
first cluster of cases was reported in Wuhan, China. causes, physical characteristics, current treatment
Four months later, the world was on lockdown. options, and ongoing research surrounding
Racing to create an effective and safe vaccine, hypophosphatasia, cleidocranial dysplasia,
global pharmaceutical companies fast-tracked mucopolysaccharidosis type I, and osteoarthritis.
vaccine development in an attempt to end the
pandemic. As of early March, the following three
companies have produced vaccines that are Effectiveness of HOCl Irrigation in Rat
authorized by the FDA for emergency use in the Peritonitis Models
United States: Pfizer and BioNTech, Moderna, and Benjamin Pomeroy
Johnson & Johnson. Despite the vaccines’ proven Faculty Advisor: Patrick Brooks
effectiveness and safety, a large population of U.S.
citizens is still hesitant to receive the vaccine. Peritonitis is an inflammatory condition effecting
Moreover, critics have raised questions regarding mesothelial cells lining the peritoneal cavity and is
the ethics of the CDC’s vaccine distribution plan, commonly induced by perforations of the bowel.
the expedited vaccine clinical trials, and the This event is a medical emergency often resulting in
government’s transparency about the vaccines. This recurring peritoneal infections and contribute to
presentation will expound on the efficacy and patient mortality. Complications may be related to
molecular mechanisms of the three authorized intra-abdominal irrigation solutions which have been
vaccines, the factors causing vaccine hesitancy reported to induce mesothelium cytotoxicity and
across the country, and the ethics surrounding the express insufficient bactericidal and bacteriostatic
vaccine and its development. properties. Consequently, hypochlorous acid (HOCl)
has been proposed as a safer and more effective
8Abstracts
irrigation solution with hopes of reducing recurrent Investigating the Effect of Microplastics and
infections and mortality rates. In this proposed study Nanoplastics on Human Health
we will compare the efficacy of HOCl and common Daniel Amuedo
irrigation solutions of saline and chlorhexidine Faculty Advisor: Richard Garrad
(CHX) on the acute treatment of induced peritonitis.
Adult Sprague Dawley rats will undergo laparotomy Plastics take on many roles and forms, and their use
surgeries where peritonitis will be induced through is nearly ubiquitous. Microplastic (MP) and
bowel perforations. Abdominal irrigation of either Nanoplastic (NP) particles have recently been
HOCl, saline, or CHX, and their efficacy will be identified in foods, air, treated water, oceans, human
assessed through abdominal and thoracic tissue fecal matter, the polar regions, and in areas
analysis. We hypothesize that use of HOCl as a untouched by direct human contact. Both MPs and
peritoneal irrigation solution in acute peritonitis NPs are primarily produced from the breakdown of
treatment will reduce bacterial presence, tissue larger pieces of plastic in rivers, lakes, oceans,
damage, and mortality rates. landfills, agriculture, industry, and urban effluent.
Additionally, plastic production has increased since
its inception, hence it is vital to investigate the
Malaria: An Epidemiology Overview potential health implications due to chronic or acute
Daniel Amuedo, Emily Chisum, Abby Gann, Hadyn MP/NP exposure. This review searched various
Tisch, Leanna Zelle databases for all pertinent articles and has distilled
Faculty Advisor: Richard Garrad the information pertinent to human health.
Generally, MPs/NPs were observed to have a dose
As of 2019 the WHO reported an annual 229 million dependent toxicity that depended upon size, shape,
malaria cases worldwide, with approximately polymer type, and associated additives. Overall,
410,000 deaths. Malaria is caused by unicellular there were many cellular changes due to MP/NP
eukaryotic parasites that belong to the genus exposure, but both an increase in ROS and
Plasmodium. Classic infection symptoms are fever, proinflammatory cytokines were observed most
chills, and flu-like symptoms. However, infected consistently across all studies. Future studies should
pregnant women are at further risk of having per- utilize aged and co-incubated MPs and NPs when
term labor with low-birth-weight babies. According testing, as virgin polymers are not representative of
to the CDC Malaria would cost at least 90 billion to circulating plastics.
eradicate but it is estimated that if the United States
contributed 5 to 6 billion incidence could be reduced
by 40 percent in under 10 years. Currently Malaria Hypothesis of Oxidative Stress and Aging
is treated by effective anti-malarial drugs; however, Tharron Roy
the treatment for malarial infection relies on the Faculty Advisor: Richard Garrad
patient's overall health, drug availability, use of
other drugs prior to infection, and on the type of The hypothesis of oxidative stress and aging
malarial infection. There are 4 different malarial postulates that phenotypic aging is induced by an
parasites present in the female Anopheles mosquito accumulation of oxidative stress within an organism.
that are infectious to humans. With anti-malarial Oxidative stress occurs when there is an imbalance
drug resistance occurring, global warming between oxidants and the antioxidants that protect
increasing the breadth of infectious mosquitos, no against them. Oxidants arise either endogenously
viable malarial vaccines, its current effect on human through normal cellular processes or from
life, and its eradication with relatively small fiscal exogenous sources. The oxidants have the capacity
contributions, it is imperative that steps are taken to to cause damage to important macromolecules
eradicate malaria soon. within a cell. Oxidative damage to DNA and other
structures within the mitochondria can trigger
mitochondrial dysfunction, halting normal energy
9Abstracts
production and further damaging the cell. This may PCR Technique to Test for siRNA Knockdown of
contribute to the development of a senescent TRIM5α
phenotype. Here we investigate the hypothesis of Emily Graham, Amy Hulme
oxidative stress and aging, support for this which is Faculty Advisor: Amy Hulme
drawn from experiments demonstrating the effects
of oxidative stress in different species. Further Human Immunodeficiency Virus type 1 (HIV-1)
analysis shows that while oxidative stress can be affects around 38 million people worldwide. The
detrimental to a cell, small amounts of oxidative HIV-1 viral capsid is a complex of 1500 capsid
stress can prove to be beneficial. This is the idea of proteins that encase the genomic RNAs. A
mitochondrial hormesis. Future directions include restriction factor called TRIM5α interacts with the
defining what level of oxidative stress may cause a capsid and alters infectivity. In rhesus macaque cells
senescent phenotype and investigating ways to delay TRIM5α binds and degrades the capsid before
the onset of senescence and age-related diseases. reverse transcription, which decreases HIV-1
infectivity. In some human cells, however, TRIM5α
has little to no effect on HIV-1 infection. To further
Neuronal Migration in Developmental test the effect of human TRIM5α on HIV-1
Hyperserotonemia: Assessment of Vesicular replication, siRNA knockdown will be used to
Glutamate in the Raphe Nuclei decrease TRIM5α expression in the human
Trey Shupp microglial cell line CHME3. The goal of this project
Faculty Advisor: Lyon Hough is to develop a PCR method to quantify the amount
of TRIM5α expression. For this method, primers for
Serotonin is involved in the early development of TRIM5α were selected based from a BLAST primer
the central nervous system and the organization of search. Each primer set was tested using PCR with
neurons throughout the cerebral cortex. It is CHME3 complementary DNA. PCR products were
proposed that serotonin indirectly acts on cells of the run on a gel and cloned into a plasmid. The prime
cerebral cortex that secrete a protein called reelin. efficiency of each primer set was determined using a
This protein is known for its role in neuronal dilution series of the plasmid. Successful primer sets
organization and migration during early neural can then be used to quantify expression of TRIM5α
development. Neuronal disorganization of the cortex in cells after the knockdown has occurred relative to
has been reported in response to increased levels of normal expression using qRT-PCR.
developmental serotonin which in turn lowers adult
serotonin and reelin levels. For reelin to be secreted,
the neurotransmitter γ-aminobutyric acid (GABA) Wastewater Testing for Sars-CoV-2
needs to be available to interact with cellular Hannah Matheney, Amy Hulme
receptors. However, GABA is associated with the Faculty Advisor: Amy Hulme
Glutamate/GABA excitatory and inhibitory
feedback system. It is known that serotonin is the The novel SARS-CoV-2 virus that causes COVID-
early regulator of glutamate release in the cell which 19 has caused over 2.6 million deaths worldwide
activates the release of GABA. However, it is since its discovery in December 2019. In Greene
thought that in conditions where excess serotonin is County alone there have been over 28,000
released during development, serotonin will inhibit confirmed cases of COVID-19. Wastewater testing
glutamate release. This study proposes to analyze has been implemented by the state of Missouri to
the correlation of excess developmental serotonin on monitor SARS-CoV-2 infection and inform public
the neurotransmitter glutamate by examining the health decisions. Infected individuals will shed virus
levels of vesicular glutamate transporter proteins in feces that can then be detected in the wastewater.
within the rat dorsal raphe nuclei. Importantly virus can be shed by infected
individuals that are asymptomatic or
presymptomatic. After wastewater collection,
10Abstracts
samples are filtered for virus sized particles, nucleic role of CypA in HIV-1 infection and capsid
acids are concentrated, RNA is isolated, and RT- uncoating within CHME3 microglial cells. We
qPCR is done to determine the number of SARS- hypothesize that CypA facilitates HIV-1 infection
CoV-2 viruses present in the sample. Over time, this and assists in normal capsid uncoating. The process
information can be used to implement testing to of uncoating will be assayed with the In Situ
identify newly infected individuals and monitor the Uncoating Assay in the presence and absence of the
state of SAR-CoV-2 infection at a population level. CypA inhibitor Cyclosporin A (CsA). For the assay,
CHME3 cells are infected with dual labeled virus
and fixed on a time course. Once fixed, samples
Effect of Cyclophilin A on HIV Infectivity undergo primary and secondary antibody staining.
Lucas Ott, Amy Hulme To make the dual labeled virus, 293T cells are
Faculty Advisor: Amy Hulme transfected with four plasmids: S15-Tomato to label
the HIV membrane, VSV-G for the envelope spikes,
HIV fuses with the cell membrane then its capsid GFP-VPR to label the viral core, and HIV-GFP to
disassembles, which is known as uncoating. The label the HIV genome and other proteins. For the
process of uncoating is poorly understood, but assay, 80% of GFP-Vpr labeled virus should also
certain cellular factors can have an impact on have S15 fluorescence. Preliminary data indicates
uncoating. Previous research has shown that that CypA facilitates HIV-1 infection and capsid
cyclophilin A (CypA) binds the HIV capsid to uncoating in CHME3 cells.
influence infectivity. CypA is inhibited by the drug
cyclosporin A (CsA). The E45A capsid mutant has a
hyperstable capsid that uncoats slower than wildtype Association Between Acne and Dietary Glycemic
HIV, while the E45A/R132A double mutant shows Index in College Students
uncoating kinetics and infectivity similar to wildtype Hannah Yates
HIV. In this project we used CHME3 cells, which Faculty Advisor: Anne Marie Hunter
express CypA, to determine the effect of CypA on
HIV infectivity. CHME3 cells were infected with Acne is a visible skin condition with the potential to
HIV-GFP wildtype, an E45A mutant, or an have negative impacts on quality of life in college
E45A/R132A double mutant in the presence of CsA students. Dietary glycemic index may have a role in
or EtOH. Flow cytometry was then used to detect acne pathogenesis. This cross-sectional study was
the amount of GFP positive cells, which represents a conducted to explore the relationship among dietary
successful HIV infection. CHME3 cells exposed to glycemic index and acne severity in a college
E45A showed very little infection in the presence of student population. Participants completed an online
CsA and EtOH. The CHME3 cells exposed to HIV- survey consisting of age, self-reported acne severity,
GFP wildtype and the E45A/R132A double mutant and food frequency questionnaire. Data from 38
in EtOH showed significant infection but showed participants was included in the analysis. Spearman
slightly less infection in the presence of CsA. These Rho correlations were performed to examine the
results indicate that CypA promotes HIV infectivity. relationship between the dietary glycemic index,
acne severity, and age. Results showed no
significant relationship between dietary glycemic
CypA Facilitates HIV-1 Infection in CHME3 index and acne severity, (P = .957.) However,
Cells via Capsid Uncoating results showed a significant, negative relationship
Emma Wise, Amy Hulme between dietary glycemic index and age, (P = .003).
Faculty Advisor: Amy Hulme This study had a relatively small sample size of 38
participants after exclusion criteria and partial
During infection, HIV-1 hijacks the cellular protein responses were accounted for. A larger, more
Cyclophilin A (CypA) to facilitate infection in some diverse study population may have yielded different
cell types. The goal of this study is to investigate the results. Providing additional information regarding
11Abstracts
the link between diet and acne may help prevent and sources. This research project focused on the
treat the skin condition linked with a lesser quality acceptability of added cricket flour to breakfast
of life. muffins. Cricket flour contains 60 grams of protein
per cup. Four types of muffins were produced using
0%, 10%, 20% and 25% cricket flour substituted for
Food Insecurity in International Students at a wheat flour. Results showed that the added cricket
Midwestern Public University flour did not adversely affect volume or height of
Anna Laffoon the muffins. Results of the consumer taste panel
Faculty Advisor: Sarah Murray indicated that taste, aftertaste and texture scores
decreased with increasing cricket flour added. Even
Food insecurity is an issue found throughout the so, the majority of taste panel participants stated that
United States. There is considerable research on all of the products were acceptable. After tasting
food insecurity and its impact on groups throughout products and reading an educational flyer about
the U.S., however, there is limited research on food cricket flour, only one participant stated that they
insecurity experienced by college students, would not consume the products in the future. 100%
specifically international students. The purpose of stated that these products would be a good choice
this study was to learn if international students at a for those wanting to consume a sustainable protein
midwestern public university were utilizing the source. It appears that substituting up to 25% cricket
campus food pantry. Students were asked to share flour for wheat flour is acceptable to increase
their thoughts and opinions related to experiences protein content.
with the food pantry. International students at risk
for food insecurity were identified by a university
student wellness assessment tool. Participants Development of Beverages Containing Beet Root
reported having a positive customer service Powder and Tart Cherry Juice Designed to
experience at the food pantry, while expressing Increase Athletic Performance
interest in additional food pantry locations, flexible Keri Dougless, Emily Monje, Jeremy Hatten
hours, and more information online. Food Faculty Advisor: Deborah Piland
preferences were related to easy to prepare foods
and snacks. Participants were particularly interested Beets are a naturally high source of nitrates which
in access to more fresh fruits and vegetables. may enhance athletic performance by increasing the
Overall, food insecure international students were efficiency of mitochondria. They also may optimize
found to be utilizing and enjoying the campus food oxygen usage and increase time to exhaustion. Tart
pantry. This information can be used to improve and cherry juice has been associated with reducing both
increase access and inclusivity of the campus food muscle and joint pain as well as decreasing the
pantry for food insecure international students. inflammatory response and encouraging recovery.
This study investigated the acceptance of a variety
of beverages designed for athletes which included
Determining the Acceptability of Savory both beet root powder and tart cherry juice. Three
Breakfast Muffins with Added Cricket Flour to beverages were developed; one juice based, one tea
Increase Protein Content and Sustainability based, and one smoothie. Taste panelists stated that
Lauren Bays, Kelsey Smith, Taylor Sands all of the products were acceptable and that they felt
Faculty Advisor: Deborah Piland they would be beneficial for those desiring to
increase their athletic performance. It appears that a
Increasing world populations place extreme pressure variety of beverages which contain beet root powder
on our land and resources to produce adequate food. and tart cherry juice are an acceptable way for
Flour made from dried and ground crickets can be athletes to optimize their performance.
used to increase the protein content of food without
the environmental cost of other animal based protein
12Abstracts
The Effect of Leadership Training on School cricket flour did not negatively affect the mean
Foodservice Employee's Job Satisfaction volume or height of the muffins. Taste panelists
Ceira Fields stated that muffins which contained up to 20%
Faculty Advisor: Deborah Piland substitution with cricket flour were acceptable but at
the 30% substitution level there noted negative
Lack of knowledge on the application of quality texture and flavor attributes. Cricket flour contains
leadership is a common issue in foodservice. This 60 g protein per cup as compared to 13 g for a cup
leads to the industry experiencing exceedingly high of wheat flour. A 10% cricket flour substitution
turnover and low job satisfaction. High turnover can increases protein content by 50%, a 20%
result in additional cost for an organization, as more substitution doubles the protein content. All but 2
supervisory time is dedicated to training, rather than panelists stated that they would consume this
leadership. Additionally, poor quality leadership product and 100% stated that they would be a good
practices often have a deleterious effect on choice for those wanting to consume a sustainable
employee morale, motivation, and job satisfaction. protein source. Results show that up to a 20%
Quality leadership, effective communication, and cricket flour substitution is an acceptable way to
fostering of healthy interpersonal relationships increase protein content in muffins.
between employees and supervisors/employees has
shown to greatly increase morale and overall job
satisfaction. This is particularly true of school Utilization of Nutrigenomics Principles to
foodservice staff, who must manage tight budgets, Develop Food Products Beneficial for Those with
state and federal regulations, and the daunting task or at Risk of Developing Type II Diabetes
of keeping hundreds of students, teachers, and staff Mellitus
satisfied with the meals. With these overwhelming Gianfranco Morote Galvez, Stephanie Urich, Tayla
tasks, staff can suffer the same struggles for Smith
motivation and job satisfaction. The aim of this Faculty Advisor: Deborah Piland
project is to develop a series of leadership training
modules to increase knowledge in emotional The goal of this project was to develop products
intelligence, leadership styles, and conflict useful for blood glucose control. This project
resolution to be presented to the kitchen managers at involved the development of products which
Springfield Public Schools in Springfield, MO. contained probiotics and prebiotics associated as
being beneficial for those with Type II Diabetes
Mellitus. Two products were developed; a fresh fruit
Determining the Acceptability of Added Cricket salsa with added inulin, a fructose polymer and a
Flour to Banana Nut Chocolate Chip Muffins in version which included dextrin, a digestion resistant
Order to Increase Protein Content and glucose oligosaccharide. Both versions also included
Sustainability a probiotic supplement which included strains
Abigail Fisher, Matthew Bertoldie, Emily Rudolph. specifically beneficial for controlling blood sugar.
Faculty Advisor: Deborah Piland The other product produced included a 100%
blueberry juice, rooibos tea containing sorbet with
Raising crickets for food requires a fraction of the probiotics also using inulin and dextrin in two
resources required to produce meat from larger different versions. Rooibos tea is the only known
animals. Cricket production require little land, water source of asphalthin, shown to help control blood
and food. The purpose of this experiment was to sugar in animal studies. Panelists stated that all of
determine the acceptability of substituting cricket the products were acceptable, and that they would
flour for wheat flour in banana nut chocolate chip consume these products, especially because of the
muffins. Four variations of muffins were produced potential health benefits. Panelists also stated these
containing 0%, 10%, 20%, and 30% cricket flour products would be acceptable for those wanting to
substitution. Results showed that substitution of control their blood sugar. This study demonstrated
13Abstracts
that the purposeful development of food products risk of heart attacks and strokes. A diet high in
which meet the needs of a specific population can be sodium intake is closely associated with elevated
useful in attaining health goals. blood pressure. The average canned or prepared
soup is high in sodium with a 1 cup serving
containing 800 mg. The Daily Value for sodium is
Development of Savory Granola Bars Which 2400 mg per day. This study investigated ways to
Contain Anti-inflammatory Phytochemical decrease the amount of sodium in soups by using
Compounds for Those with Rheumatoid Arthritis salt substitute, potassium chloride (KCl), as well as
Emma Sesti, Jessica Jennings, Elizabeth Knipp several types of sea salts. Five variations of tomato
Faculty Advisor: Deborah Piland basil soup were prepared using 100% table salt, 50%
table salt/50% KCl, 50% Black Lava salt, 50% Pink
Rheumatoid arthritis (RA) is an autoimmune Himalayan salt, and 50% of evaporative sea salt
inflammatory disease that mainly attacks the joints. from Mexico. Results showed that taste panelists
Autoimmune diseases occur when the body’s rated all of the soups, except for the soup made with
immune system mistakes its own tissues for foreign salt from Mexico, as acceptable. 100% of
invaders, such as bacteria or viruses. RA affects 1.3 participants stated that these soups would be
million Americans. Numerous phytochemicals appropriate for those wishing to decrease their
found in fruits, vegetables, nuts, seeds and spices sodium intake. It appears that utilizing up to 50%
have been shown to significantly reduce KCl, and using ½ of the recipe amount of Black
inflammation. This study focused on developing Lava and Pink Himalayan salt are acceptable ways
savory granola bars which contained beneficial anti- to prepare soup with at least 50% less sodium.
inflammatory phytochemicals for those with RA.
Three savory granola bars were developed which
contained known anti-inflammatory compounds. Anatomical Reconstruction of an Exceptionally
Sensory panel results indicated that savory granola Large Triceratops: Insights Into the Life History,
bars were an acceptable source of these anti- Pathology, and Body Proportions of an
inflammatory compounds and the majority of Exemplary Dinosaur
panelists stated that they would consume these Lindsey Wilson, Henry Tsai
granola bars. Panelists also reported that these Faculty Advisor: Henry Tsai
granola bars would be beneficial for those with RA
who desired to consume an anti-inflammatory diet. Ceratopsians, or horned dinosaurs, were among the
It appears that savory granola bars which include a most abundant large-bodied land animal in the late
variety of anti-inflammatory food ingredients are an Cretaceous period (66 million years ago).
acceptable way to increase anti-inflammatory agents Triceratops represent the largest and latest surviving
in the diet of those with RA. ceratopsian. This study describes a new skeleton of a
Triceratops (MINS v-1036) discovered in the Lance
Formation (Wyoming), currently housed in the
Acceptance of Tomato Soup Produced with 50% Missouri Institute of Natural Sciences. We
Less Sodium Levels Developed to Help Reduce identified, measured, and 3D captured skeletal
Blood Pressure Levels in Adult Americans elements of MINS v-1036 and compared it with
Audrey Williams, Alyssa Fleming, Meghann other ceratopsian skeletons. Limb dimensions of
Robertson MINS v-1036 approach the upper range among
Faculty Advisor: Deborah Piland known ceratopsians, making it one of the largest
horned dinosaurs yet discovered. Additionally,
According to the American Heart Association more MINS v-1036 possessed skeletal features that
than 103 million Americans have high blood suggest it had reached an advanced stage of
pressure. This represents one-half of the adult senescence at the time of death. To better understand
population and untreated hypertension increases the its life history, we extracted core samples from the
14You can also read
