MRSA results in hours, not days - 3M BacLite Rapid MRSA Test
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3M BacLite Rapid MRSA Test ™ ™ MRSA results in hours, not days
Rapid MRSA identification can
help hospitals take control
Rapidly identifying Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of healthcare associated
infections worldwide. The need for rapid patient screening for MRSA as a component of infection
control measures to potentially improve outcomes in the colonised patient and prevent transmission
MRSA colonised patients
to other patients has been clearly established1.
provides hospitals with • More than 53 million people worldwide carry MRSA2
• MRSA-related hospital stays average 12 additional days3
another tool to assist in • MRSA-related infections cost an additional $27,000 - $34,000 to treat3,4
• Rapid screening may save up to 90% of isolation-associated costs5
proactively managing
New DNA-based tests, while fast, have proven cost-prohibitive for widespread use. Traditional
Health Care Associated methods rely on time-consuming culture techniques which require 48 hours to exclude MRSA
and days to confirm positives. During this time, infection control measures, such as isolation and
antibiotics, may be applied unnecessarily.
Infections, which may
3MTM BacLiteTM Rapid MRSA Test allows fast, affordable
help to improve patient screening of patients for MRSA – in hours, not days –
providing hospitals and clinicians with a valuable
outcomes and decrease tool to help positively impact infection
control practices. The rapid result
treatment costs. allows more effective use of
hospital isolation resources
while helping reduce
cross-contamination,
ultimately reducing
the time and costs
associated with MRSA.
The 3M™ BacLite™ MRSA Test Infection prevention has taken a step forward with the application of adenylate kinase (AK) Rapid
accurately identifies MRSA – technology for medical diagnostics. Through a simple test using familiar methodology, 3M BacLite
Rapid MRSA test is a qualitative assay for the detection of MRSA from nasal and groin swabs. The
in hours, not days
BacLite Rapid MRSA test gives hospitals a tool to easily and affordably determine which patients
• Qualitative negative result are most at risk due to MRSA colonisation, allowing hospitals to streamline laboratory workflow,
in five hours reduce costs associated with unnecessary isolations and improve management of microbial
infections – potentially improving patient outcomes and mitigating risk of cross-contamination.
• Proprietary AK Rapid ®
technology delivers 45 minutes total hands-on time for 43 direct from patient specimens
reliable results 1. Screening swabs are collected from patients using standard hospital procedures
2. Dispense pre-warmed & supplemented broth into Bijou, add swab and vortex
• Diagnostic sensitivity 3. Prepare processor and reader
of 94.6% for MRSA 4. Dispense capture reagent and sample into microtitre plate and wash in the processor
nasal detection * 5. Add controls and read
• Diagnostic specificity 3M BacLite Rapid MRSA Test kit components
of 96.9% for MRSA • Reagent Pack
nasal detection * • Supplement Pack
• Broth Pack
• Negative predictive
• Microtitre Plates
value of 99.9% for MRSA
nasal detection *
Conventional broth based microbiology techniques with AK bioluminescence end point detection
Selective Extraction Selective Read
Enrichment & Growth Lysis
*Refer to BacLite Rapid MRSA test package insert for detailed product information.
Through an easy-to-run test A powerful new tool in the prevention and control of healthcare
– 45 minutes total hands-on associated infections.
time for 43 direct from patient Designed to provide hospital staff with rapid, reliable diagnostic information, the 3M BacLite Rapid
MRSA Test is a highly sensitive test that delivers a negative predictive value of 99.9% for MRSA
specimens – the 3M™ BacLite™
nasal detection in just 5 hours.*
Rapid MRSA Test pinpoints
patients at increased risk due to The 3M BacLite Rapid MRSA Test utilises highly sensitive AK technology:
colonisation. • Unique test measures adenylate kinase activity
• Highly affordable compared to the costs associated with molecular methods
The 3M BacLite system consists • Easy to use with minimal training required
of a Reader, Processor, Computer,
Adenylate kinase, an essential housekeeping enzyme found in all cells, is used as an ultra
Printer and Barcode Scanner.
sensitive cell marker. AK regulates energy provision by catalysing the equilibrium reaction: ATP
• Highly sensitive rapid culture + AMP 2ADP. By supplying purified ADP in vitro, the amplified levels of ATP produced can be
based screening test measured using bioluminescence.
• Results direct from specimen This amplification means that AK Rapid technology is up to 100 times more sensitive than
traditional ATP based systems. In addition, because AK levels within cells fluctuate less than ATP
• Automated end-point with
levels do, correlation of the AK assay with cell numbers is more accurate than that given by direct
objective results
ATP-based measurements.
• Barcode for effective sample
tracking This approach retains the benefits of traditional culture-based methodologies, including ease of
use, but provides a result more sensitive than traditional optical techniques in just 5 hours and
• Easily expanded to run without the high cost associated with DNA-based methods. This allows hospitals to affordably
additional BacLite tests implement screening to help optimize patient outcomes and mitigate the risks associated with
cross-contamination.
*Refer to BacLite Rapid MRSA test package insert for detailed product information.
3M™ BacLite™ Rapid MRSA Test
Fast Turnaround
• Confirmed negative in just 5 hours; confirmed positive next day
• Easy to implement as part of pre-admittance
Easy to use
• Swab used for specimen collections
• Minimal training required to run test
• Automatic interpretation of results
Highly Sensitive AK Rapid Technology
• Detects only viable organisms
• Bioluminescence combined with stable adenylate kinase
for fast, accurate results
Nasal Swabs - Diagnostic Sensitivity
and Specificity*
Sensitivity 94.6%
Specificity 96.9%
NPV 99.9%
Groin Swabs - Diagnostic Sensitivity
and Specificity*
Sensitivity 95.9%
Specificity 88.8%
NPV 99.9%
* Refer to the 3M BacLite Rapid MRSA test package insert for detailed
product information.
Order Code Product Description
GH620500310 3M™ BacLite™ Flex System Reader, Processor, PC, Monitor, Printer,
Bar Code Scanner and Accessories
DH999995672 3M™ BacLite™ MRSA Sample Swabs Box of 125 Swabs
GH620500328 3M™ BacLite™ Rapid MRSA+ 1 Set of Test Reagents
Pack 1: Test Reagents
GH620500336 3M™ BacLite™ Rapid MRSA+ 10 Sets of Broth Supplements
Pack 2: Broth Supplements
GH620500344 3M™ BacLite™ Rapid MRSA+ 10 Bottles of Broth
Pack 3: Broth
GH620500351 3M™ BacLite™ Rapid MRSA+ 10 Microtitre Plates and Seals
Pack 4: Microtitre Plates and Seals
References:
1 Salgado CD, Farr BM. “What proportion of hospital patients colonized with methicillin-resistant Staphylococcus aureus are identified by clinical microbiological cultures.”
Infect Control Hosp Epidemiol. 2006;27:116–21
2 Okuma K. “Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community”. J Clin Microbiol 40 (11): 4289-94. PMID 12409412
3 Abramson MS. Infect Control Hosp Epidemiol. 1999; 20: 408-11
4 “New Research Estimates MRSA Infections Cost U.S. Hospitals $3.2 Billion to $4.2 Billion Annually” Study conducted by U.S. Outcomes Research Group, Pfizer, Inc.,
Abstract # 9489
5 Bootsma MCJ, et al. “Controlling methicillin-resistant Staphylococcus aureus: Quantifying the effects of interventions and rapid diagnostic testing.” Proceedings of the
National Academy of Sciences of the United States of America. 2006; 103: 5620-5625
3
3M Medical Diagnostics
3M Health Care Limited 3M Ireland
3M House 3M House
Morley Street Adelphi Centre © 3M 2007
Loughborough Dun Laoghaire 3M and BacLite are trademarks of 3M or Acolyte Biomedica Ltd.
Leicestershire LE11 1EP Co Dublin AK Rapid is a registered trademark of Defense Science and
Phone: 01509 611611 Ireland Technology Laboratory.
Fax: 01509 237288 Phone: (01) 280 3555 Date of preparation: November 2007.
Web: www.3m.co.uk/medicaldiagnostics Fax: (01) 280 3509 GW001You can also read
