Bioprocessos para el aprovechamiento de los desechos de cultivos multifunzionale - Citta del ...
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Bioprocessos para el aprovechamiento de
los desechos de cultivos multifunzionale
Territorial and Production Systems Sustainability Department (SSPT)
Biotechnology and Agroindustry Division
Bioproducts and Bioprocesses Laboratory (PROBIO)
Dr Loretta Bacchetta
Presentation outline
Overview of the aims and activities at ENEA Biotechnologies and
Agroindustry Division BIOAG– Bioprocess and Bioproducts Laboratory
BIOAG inputs for cultural heritage conservation
Examples of on-going research projects:
Genetic resources characterization: hazelnuts as model of study
Bioraffinery: Cynara spp. for food and non food applications
Bio-fungicide: enhancement of buckwheat by-products
The Mild technologies at PROBIO laboratory
Efficient use of resources: biobased products from cladodi of Nopal for
Cultural Heritage applications
Final considerations
Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November2017
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Biotechnology and Agroindustry Division-BIOAG
Focuses on innovation in the agro-industrial system for the development and
competitiveness of food production in terms of quality, safety and
sustainability, encouraging chain approaches, through agro-industrial and/or territory
clusters.
Promotes the development of products (food and non-food) with high-added
value and high technological value derived from biological resources.
Develops methods and technologies for innovation and traceability of the
production, supply, processing and marketing chains of agricultural and food products
in a perspective of enhancing consumer confidence in the foodchain.
All activities are performed through innovative infrastructures, equipment, facilities, and technology/service
platforms, located in three different laboratories:
BIOTECHNOLOGY
BIOPRODUCTS AND BIOPROCESSES
SUSTAINABILITY, QUALITY AND SAFETY OF
AGRIFOOD PRODUCTIONS
Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November2017 3
Biotechnology and Agroindustry Division activities
Innovative biotechnology products and Systems for sustinable quality safety of
processes and systems for Bioeconomy Agroindustrial productions
• “Omic” sciences and new breeding techniques • Conservative and precision agriculture for
in Mediterranean crops sustainability and phytosanitary measures for crop
• Conservation, characterization and evaluation protection. Agriculture-based models, to improve
of existing biological collections including the resilience to climate change and to promote
microrganismsf or industrial purposes (food and reduction of inputs and greenhouse gas emissions
biotechnology) • Precision indoor farming and agrospace
• Enhancement of waste and agro-industrial by- • Use of renewable sources and improvement of
products, into high added-value products
(innovative food, feed, materials and bio-based the food system throughout a cascade-type
products) and focusing on the resources efficient approach, planning for maximal biomass
use, use of Key Enabling Technologies (KETs), exploitation and agri-food waste and for reducing
industrial biotechnology (White Biotechnologies) food losses.
and Process Technologies (with a special focus on • Validation and application of diagnostic methods
Mild Technologies), for agroindustry and bio- for food safety, qualification of the productions
industry ,processing, preservation and packaging. Quality
markers for authenticity and origin of foods
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Bioprocesses– Meeting Ambasciata Italiana in Mexico Bilateral Project, November, 2017
"Bioproducts and Bioprocesses" Laboratory (PROBIO)
Aims to develop innovative products and processes in food and no-food areas
through the use of Key Enabling Technologies (KETs). Special attention is devoted to
developing industrial biotechnologies (White Biotechnologies) and Mild Technologies,
in agroindustry and bio-industry areas.
The activities are: to detection, at different levels of scale, of
innovative and sustainable productive solutions, able to
obtain a wide range of products /processes bio-based, with
high added value of interest for new markets. The
approaches allows the valorization of biological resources sspt.enea.it
use, vegetables matrices, by-products and waste. roberto.balducchi@enea.it
The Laboratory combines expertise on valorization of vegetables, animal and microbial resources (agronomy,
microbiology, entomology, chemistry, veterinary, environmental sciences), industrial biotechnology, chemical
engineering, Nuclear Magnetic Resonance (NMR), biochemistry.
Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November2017
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BIOAG inputs for cultural heritage conservation
Agro-
Recovery biodiversity
biomolecules
from waste
Methodologies
of chemical
extraction –
MILD
TECNOLOGY
Biomortars
Biocide additives
Bio-processes– Meeting Ambasciata Italiana in Mexico Bilateral Project, November, 2017
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Genetic resources characterization
hazelnuts (Corylus avellana) as model of study
Bioprocessi – Meeting Ambasciata Italiana in Mexico November2017 7
Corylus avellana variability
Boccacci P., et al., Bacchetta L., 2013. Tree Genetics & Genomes 9:1465-1480 Rovira M., et al, Bacchetta L.,2013. Annals and Nutrition metabolism 62 (2), 82
SAFENUT: Agri Gen Res 068 Variability in polyphenolics
215 cultivars e 58 accessions are amintaned in 13
European collections
190 are characterized by SSR.
42 out of 140, ecotypes recovered ‘on farm’ were
confirmed new genotypes
18 phenolics identified in 53 cultivars: 10 flavonoidi (quercetin
pentoside, quercetin 3-O-rhamnoside, catechin, epicatechin,
myricetin-3-rhamnoside, procyanidin dimer-1, procyanidin dimer-2,
procyanidin trimer-1, -2 and -3) 7 phenolics acids (gallic,
protocatechuic, chlorogenic, p-coumaric, ferulic, ellagic and caffeic
acid); 1 dihidrochalcone (phloridzin).
Bioprocesses– Meeting Ambasciata Italiana in Messico, Bilateral Project, Novembre 2017 8
Study of Corylus avellana variability
Bacchetta et al., 2013. Euphytica 191: 57-93 Drogoudi, P.,et al., Bacchetta, L, 2013. J. Food Sci. Nutr.,64, 202–209.
Variabiliuty in fatty acids Variability in proteins and minerals
Analizzate 75 cultivars
Bioprocessi – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembre 2017 9
Fuctional food: clinical trials
Asım Orem, et al. (2013). Hazelnut-enriched diet improves cardiovascular risk biomarkers beyond a lipid-lowering effect in
hypercholesterolemic subjects. Journal of Clinical Lipidology, 7, 123-131.
Tey, S.L., et al. (2011). Effects of different forms of hazelnuts on blood lipids and α-tocopherol concentrations in mildly
hypercholesterolemic individuals. European Journal of Clinical Nutrition 65, 117–124.
Mercanligil, S.M., et al. (2007). Effects of hazelnut-enriched diet on plasma cholesterol and lipoprotein profiles in
hypercholesterolemic adult men. European Journal of Clinical Nutrition, 61, 212-220.
Fraser, G.E., et al. (1992). A possible protective effect of hazelnut consumption on risk of coronary heart disease. The Adventist
Health Study. Arch Intern Med, 152, 1416–1424
BIOSERVICE
Project
Lr 13/2008 PROT. FILAS-
RU-2014-1168 - 2018
Activities
CUORESANO
Supported by
COOPERNOCCIOLA srl
(Viterbo)
Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November,2017 10Fuctional food: results of clinical trial
+ 251,2 Kcal/d
No variation
in weight
reversible
benefits
Bioprocesses– Meeting Ambasciata Italiana in Mexico,
11
Bilateral Project, November 2017Improvements on chemical/molecular characterization
Metabolomics ESOSOMES
Developped of protocol for isolation of esosomes
ENEA e CNR –Biologia Cellulare e Neurobiologia
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembre 2017 12Genetic resources characterization: research activities in progress
Fagopyrum spp. Brassica rapa local ecotypes
Cynara cardunculus var. scolymus
esculentum tataricum sulforaphano (µ g/g ps)
RUTINA
Gluten free
Ocimum
basilicum Wild edible plants Bacchetta et al., 2016.
A manifesto for the
valorization of wild
edible plants.
Pinus pinea J.Ethnopharmacology
191:180-187
Bioprocesses – Meeting Ambasciata Italiana in Mexico, 13
Bilateral Project, Novembre 2017CYNARA spp
Breeding for new varieties address to food and non food industry
Bioprocesses– Meeting Ambasciata Italiana in Mexico, Bilateral Project, November 2017 14Artichoke and Cardoon: strategy
Morphological
FOOD
characterization PRODUCTION
(International descriptors )
(artichoke)
Selection
Molecular Assisted
characterization
breeding Biomass
( ISSR & AFLP)
production
biopolimers
Biocheical (cardoon)
characterization
(ASE extraction and
HPLC analysis)
paola.crino@enea.it
Bioprocessi – Meeting Ambasciata Italiana in Mexico November 2017 15Artichoke and Cardoon
Settore FOOD - Artichoke NON FOOD- Cardoon
Michelangelo Donatello
Patent : 3
Raffaello ARTICHOKE
VARIETIES for
edible consumption
and characterized by
good nutraceutical
properties. • Development of genetically stable cardoon
lines. bioactive compounds - chlorogenic acid,
1,5-O-1,5-O-dicaffeilquinic acid, cynaroside
Bioprocessi – Meeting Ambasciata Italiana
16
in Mexico November 2017Aspergillus
Rutin
flavus
Buckwheat Crusca
BIO-FUNGICIDE
Enhancement of Fagopirum spp by-products
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017
17Fagopyrum Effects of extracts from bran of Infection on corn of A flavus
esculentum and F. buckwheat on di Aspergillus
tataricum flavus growth
chiara.nobili@enea.it
Minor crop
Gluten free
High ammount of amino-acids, fibre,
vitamine, minerals, polyphenols
The use of the “mild technologies” (supercritic CO2) allowed to obtain
exstracts enriched by lipids and phenolics
Study on functional properties in bread, The extracts inhibited the Aspergillus flavus as well growth in vitro as
crackers enriched with buckwheat aflatossine B1 e B2 production.
Bioprocessi – Meeting Ambasciata Italiana Mexico November 2017 18The Mild Technologies Laboratorio PROBIO Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017 19
MILD TECHNOLOGIES – LAB PROBIO
Supercritic extraction Membrane separation and filtration
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017 20'…ìl fico d'india un tesoro sotto le spine'
Opuntia ficus indica
Efficient use of resources: biobased products from cladodes for
Cultural Heritage conservation
Bioprocesses – Meeting Ambasciata Italiana in Mexico,
Bilateral Project, Novembrer, 2017 21Proyecto Bilateral México-Italia - Objectivos
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017 22Opuntia ficus indica cultivo en Italia
Cultivo de Opuntia ficus
indica en Sicilia Cultivo de Opuntia ficus
40, 000 ettaros-Sicilia indica en
Total rendimiento Sicilia (San Cono)
del fruto: 86, 000 toneladas
Los cladodios no se usan y son un
"desperdicio“
130-150 quintali biomasa/ettaro
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017
231. Localización de mucilago en el cladodio de nopal
Epidermis
Clorénquima
Parénquima
El clorénquima y parénquima contiene idioblastos
que contienen mucilago
El “clorénquima” es rico en cloroplastos
El parénquima además de mucilago, se sintetizan
alcaloides, fitohormonas y es rico en amiloplastos
Células especializadas (idioblastos)
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017
242. EXTRACCIÓN CON AGUA- Método tradicional
Factores críticos ANÁLISIS MULTIVARIADO
• EFFECTO DE LA DILUCIÒN • El análisis multivariante ha
• EFFECTO DEL PH DEL AGUA permitido el desarrollo de un
• EFFECTO DEL LA FUERZA modelo de previsión para obtener
IÒNICA mucílagos de acuerdo a los
factores críticos
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017
25CONCLUSIONI
SINERGIES AND
COLLABORATIONS FOR THE
DEVELOPMENT OF BIO- loretta.bacchetta@enea.it
ECONOMY, CIRCULAR
ECONOMY AND
SUSTAINABILITY OF
PRODUCTION PROCESSES
OPEN NEW HORIZONS
MAKING MORE COMPLETE
AND EFFECTIVE THE
RESEARCH ACTIVITIES
Bioprocesses – Meeting Ambasciata Italiana in Mexico, Bilateral Project, Novembrer, 2017
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