Company presentation Website - Aug 19th, 2019 - White Dog Labs
←
→
Page content transcription
If your browser does not render page correctly, please read the page content below
Company presentation
Website
Aug 19th, 2019
Proprietary and confidential information of White Dog Labs, Inc
Company Presentation Outline
• Overview
• Company
• Executive team
• Board of directors
• Advisors
• Investors
• Locations
• Technology
• Products
• Company organization
• Animal Nutrition – ProTyton, MiruTyton
• Animal Health – BioTyton
• Human Nutrition and Health
• Biochemicals
• Production
• Ethanol plant repurposing
• NuMake-1 at MRE, Nebraska
Overview of White Dog Labs
Biotechnology company focused on animal health and nutrition today –
humans tomorrow
World class expertise and IP in microbiome Clostridia
Products in commercialization – the “Tyton” platform of nutrition
and health solutions
Animal Nutrition
• ProTyton™ - Premium protein ingredients for aquaculture
• MiruTyton™ - Naturally derived butyrate feed additive
Animal Health
• BioTyton™ - Poultry in-ovo biologic that addresses antibiotic
reduction
3
Proprietary and confidential information of White Dog Labs, Inc
Products for Animal Nutrition and Health
Animal Nutrition co-products Animal Health
ProTyton MiruTyton BioTyton
A scalable and sustainable, Natural butyrate feed-additive with Live bacteria injected into the egg (in-ovo
premium Single Cell Protein demonstrated swine and poultry benefits. biologics) that addresses antibiotic reduction
(SCP) with demonstrated Disruptive economics, as it is a co-product of in poultry while also delivering novel
health in marine applications ProTyton fermentation hatchery benefits
Large and Growing Target Markets
MiruTyton
BioTyton MiruTyton
ProTyton
Company Presentation Outline
• Overview
• Company
• Executive team
• Board of directors
• Advisors
• Investors
• Locations
• Technology
• Products
• Company organization
• Animal Nutrition – ProTyton, MiruTyton
• Animal Health – BioTyton
• Human Nutrition and Health
• Biochemicals
• Production
• Ethanol plant repurposing
• NuMake-1 at MRE, Nebraska
Executive Team
Dr. Bryan Tracy Dr. Aharon (Ari) Eyal Dr. Shawn Jones Dr. Randy Phillips Dr. Alon Karpol
CEO and co-founder CSO and Chair of Scientific VP of Molecular and Micro Director of Fermentation GM, WDL Israel
Advisory Board Biology Technology
PhD Chemical and Former Professor of PhD Chemical and PhD Chem Eng, PhD in Biochemistry.
Biological Engineering. Applied Chemistry at the Biological Engineering, 25 years of syngas Former CEO of Designer
Jerusalem Univ. World leading collaborations Energy, innovator in
Co-founder and CEO of fermentation. Lead
renowned expert in with corporate cellulosic hydrolysis
Elcriton a biology and advanced separation engineer of BRI Tech –
fermentation company science and is the author R&D partners acquired by Ineos Bio.
acquired by WDL. of some 80 publications,
and 250 patents.
Board of Directors
Dr. Sass Somekh Talli Somekh Dr. Bryan Tracy
Executive chairman Co-Founder CEO and co-founder
30-year executive career of product and Software and biotech Co-founder and CEO of Elcriton a
business development in the entrepreneur, General biology and fermentation
semiconductor equipment industry. Partner, Musea Ventures company acquired by WDL.
Silicon Valley Engineering Hall of Fame CEO of Erisyon
8
Advisors
Prof Sam Rochell Dr. Shaymaa Abousaad
Dr. James Martin Odom
Poultry nutrition PhD, Faculty member Minufia Univ, Egypt
Biotechnology and
Univ of Arkansas Visiting researcher NCSU
Aquaculture Current research focuses on in ovo feeding
30-year career scientist at DuPont Research interests in the formulation
of broilers diets and their influences programming, mitochondria biogenesis and
– 10 years in aquaculture dynamics, autophagy mechanism, orexin and
(Norferm) and with Omega-3 on intestinal physiology, immunology,
and microbiology in broilers mitotic activity of the muscle-satellite cells.
Verlasso salmon
Dr Enav Bar-Shira Prof Ed Bayer
Prof Ronnie Friedman
PhD, Immunology researcher Chair of Bio-Organic Chemistry
Dean 2006-2013
Animal Sciences Weizmann Institute
Animal Sciences
The Hebrew University of Jerusalem co-discovered the Cellulosome - an intricate
The Hebrew University of Jerusalem
Research focuses on Development and adaptations multi-enzyme complex produced by many
Immunology of the digestive tract in precocial
of innate immunity in the gastrointestinal tract of cellulolytic anaerobic microorganisms, and in
animals. Maternal immune protection of the
the newly hatched chick, and establishment of 1994 Bayer proposed an original concept for
gut in birds. Pro-inflammatory responses to
immune competence in the avian GALT during the the construction of designer cellulosomes.
physiological stress
immediate post-hatch period
Investors
The Somekh Family Investment armWhite Dog Labs Locations
Nebraska – ProTyton & MiruTyton Production
Reached agreement with MRE ethanol plant
• 2020 - Production demonstration plant
• 2021 - Production plant
Delaware - HQ
• R&D & product/business development
• 18,000L fermenter pilot line
Israel –R&D
• Microbiome R&D
• Biologics developmentCompany Presentation Outline
• Overview
• Company
• Executive team
• Board of directors
• Advisors
• Investors
• Locations
• Technology
• Products
• Company organization
• Animal Nutrition – ProTyton, MiruTyton
• Animal Health – BioTyton
• Human Nutrition and Health
• Biochemicals
• Production
• Ethanol plant repurposing
• NuMake-1 at MRE, NebraskaWDL’s Staff – Leaders in Isolating, Analyzing, Cultivating and Engineering Clostridia
14
WDL’s Tyton Technology Platform
Discovered and isolated
Target microbiome microbiome-derived Clostridia Processing
Solventogenic Clostridia
Phage DNA
Protocol-B™ MixoFerm™
Sulfur reducing Clostridia
Acetogenic Clostridia
Acidogenic Clostridia
Previously uncultured Clostridia
Other Clostridia
Protocol-B allows the MixoFerm fermentation
discovery and isolation consumes sugar and gasses,
of new microbiome resulting in greater
based solutions for product yields.
animal and human healthAnaerobic Bioprocessing Expertise
Discovery Demonstration
• >400 unique libraries of organisms
• Discovery 5 new microorganisms per week
• Full suite of genetic engineering tools • 18,000L fermentation with cell recycle potential
• In silico capabilities • Complete bioprocessing capabilities (fermentation, cell
• Advanced analytics and strain characterization separation, washing, drying, evaporation, etc.)
capabilities • Product development under full anaerobic conditions
through packaging
WDL owns and operates facility built for obligate anaerobe handlingMixoFerm™ Technology – Higher Yield Fermentation
Conventional fermentation: MixoFerm fermentation:
Part of the sugar is converted into gas The evolving gas is consumed with the sugar
Watch video Watch video
Note the bubbles due to gas evolution Note the lack of gas bubbles
MixoFerm™ - more product for the same amount of sugarCompany Presentation Outline
• Overview
• Company
• Executive team
• Board of directors
• Advisors
• Investors
• Locations
• Technology
• Products
• Company organization
• Animal Nutrition – ProTyton, MiruTyton
• Animal Health – BioTyton
• Human Nutrition and Health
• Biochemicals
• Production
• Ethanol plant repurposing
• NuMake-1 at MRE, NebraskaProduct Based Business Units
WDL – parent company
Human Nutrition & Health
a-b-eBio Chemicals
• ProTyton • BioTyton • Protein • Acetone/Isopropanol
• MiruTyton • Probiotics • Probiotics • Cellulosic hydrolysis
Currently in commercialization Currently in product developmentA Business Unit of White Dog Labs
Aquaculture Requirements
Aquaculture companies are actively seeking an alternative premium protein that is scalable,
has greater certainty of supply, and is renewable.
Currently the only premium protein choice is fishmeal.
The annual fishmeal production is about 5 million metric-ton.
Peru’s fishmeal price impacted by El Nino 2009-2019
Catching fish
to feed fish
is not scalableProTyton™ is Best in Class
90
80 84% CP Crude Protein (CP)
70 Essential Amino Acid (EAA)
60
50
As-is wt%
40 40% EAA
30
20
10
0
ProTyton is a premium protein-ingredient that complements lower cost ingredients,
reduces fishmeal inclusion, provides health benefits and is renewable and scalableProTyton Lowers Costs and Provides Health Benefits
ProTyton allows incorporation of lower cost ingredients, and thus ProTyton decreased shrimp mortality challenged with Vibrio
lowers total feed cost even when priced higher than fishmeal at parahaemolyticus (aka Early Mortality Syndrome - EMS).
$1750/metric-ton.
Thermal Feed Compounded
Salmon Diet Growth
Coefficient
Conversion
Ratio (FCR)**
Feed
$/Metric Ton
20% ProTyton
has 1/3
mortality of
Reference
20% Fishmeal
commercial
Fishmeal 0.23 0.86 $1250 diet optimized
0% ProTyton
@$1600 for EMS
ProTyton
10% Fishmeal
resistance
@$1750/ 0.22 0.83 $1220
7.5% ProTyton PL Raceway 40-9
metric ton
USD1.85/Kg ProTyton inclusion in reference diet
*TCG = Rate of Growth **FCR = Feed/Unit of Growth. Lower is Better.
ProTyton is a premium nutrient with significant health benefitsProTyton Qualification by Customers
WDL’s 18.000L pilot fermenter Shipments for customer qualification
First offtake negotiated
with a major customerMiruTyton is a Co-Product of ProTyton
MiruTyton
Natural butyrate feed-additive with
demonstrated swine and poultry
benefits. Disruptive economics, as it is
a co-product of ProTyton fermentationButyrate – An Important Feed-Additive
Implications of butyrate and its derivatives for gut health and animal production
Andrea Bedford and Joshua Gong∗, Guelph Research and Development Centre, Canada
Abstract
Butyrate is produced by microbial fermentation in the large intestine of humans and animals. It serves as not only a
primary nutrient that provides energy to colonocytes, but also a cellular mediator regulating multiple functions of gut
cells and beyond, including gene expression, cell differentiation, gut tissue development, immune modulation,
oxidative stress reduction, and diarrhea control. Although there are a large number of studies in human medicine
using butyrate to treat intestinal disease, the importance of butyrate in maintaining gut health has also attracted
significant research attention to its application for animal production, particularly as an alternative to in-feed
antibiotics. Due to the difficulties of using butyrate in practice (i.e., offensive odor and absorption in the upper gut),
different forms of butyrate, such as sodium butyrate and butyrate glycerides, have been developed and examined for
…demonstrate positive effects on animal production, including enhancement of gut development,
their effects on gut health and growth performance across different species. Butyrate and its derivatives generally
control of enteric
demonstrate pathogens,
positive reduction
effects on animal of inflammation,
production, including enhancement improvement of growth
of gut development, control ofperformance
enteric
pathogens,
(including reduction
carcass of inflammation,
composition), andimprovement
modulation of growth
of gutperformance
microbiota. (including carcass composition), and
modulation of gut microbiota. These benefits are more evident in young animals, and variations in the results have
been reported. The present article has critically reviewed recent findings in animal research on butyrate and its
derivatives in regard to their effects and mechanisms behind and discussed the implications of these findings for
improving animal gut health and production. In addition, significant findings of medical research in humans that are
relevant to animal production have been cited.
Butyrate has nutritional and health benefits for all animals!
Its application depends on the performance/cost ratio for the end user 26MiruTyton Improves Broiler’s Growth and Health
Feed Trials
conducted at
Day 21 Weight Male Feed Conversion Ratio Males Ileum Height Males
920 1.50 760
Female Females Females
900
1.45 740
880
1.40 720
860
840 1.35 700
820
1.30 680
800
1.25 660
780
760 1.20 640
0 250 500 1000 2000 0 250 500 1000 2000 0 250 500 1000 2000
Butyrate Dose (ppm) Butyrate Dose (ppm) Butyrate Dose (ppm)
1000ppm butyrate optimal dose for growth Improved FCR for all doses 1000ppm butyrate supports best ileum health
• Males – 4% more growth • Males – 12pt improved FCR at 1000ppm • Males – 5.2% taller ileum
• Females – 5.7% more growth • Females – 5pt improved FCR at 500ppm • Females – 2.5% taller ileum
These results have encouraged a major US poultry
integrator to start qualifying MiruTyton 27Gain in Weight of Weaned Piglets
MiruTyton level
Pigs quickly take to butyrate feeds
0 ppm
during most stressful transition time.
30% weight gain for 15000ppm vs 300 ppm
control in the first 2 weeks
3000 ppm
15000 ppm
Post butyrate feeding
(residual effect)MiruTyton – A Highly Competitive Butyrate Feed Additive
MiruTyton is natural butyrate
(33wt%) that can be used with
organic livestock • Butyrate market has several options, all high priced
• Typical prices are $4,000-7,000/ton (30wt% butyrate)
• These products are “protected” and thus require smaller dose than
MiruTyton
• MiruTyton provides same performance when priced at
$1300-2300/ton, but can be priced as low as $600/ton
• WDL believes that MiruTyton will fundamentally change
the Butyrate market and will substantially grow it due to
economics
Price flexibility allows greater return to the end
user and opens-up huge new global market 29A Business Unit of White Dog Labs 30
BioTyton is an In-Ovo Biologic
BioTyton in-ovo biologics is a new class of natural, antibiotic
replacements that go beyond probiotics by “priming” the
immune system and delivering novel hatchery benefits.
In-ovo
small volume, high-value,
one-time injection into the egg
15 billion eggs are injected
annually with vaccineBioTyton improves hatch efficiency
and inhibits infectionIn-Ovo BioTyton Delivers Hatch Benefits
Poultry industry is continuously striving to increase hatch yield and decrease early mortality.
BioTyton hatch yield performance* Post hatch mortality in a contamination event**
100%
35%
90%
30%
80% 25%
% Hatchability of 70% 20%
Fertile Eggs 15%
60%
10%
50%
5%
40% 0%
10^1 10^3 10^5 10^7 Saline 10^1 10^3 10^5 10^7 10^9
Dosage (CFU/egg)
BioTyton
Saline (control) **While in-ovo vaccination is wide spread, the point of injection creates an entry way
for pathogens - a single contamination can shed the infection to the entire flock during
*SanusBio works with Prof Peter Ferket, North Carolina State early days of growthIn-Ovo BioTyton Speeds Up Growth
A primary benefit of antibiotics is faster growth. Replacements should aim to do the same
Faster growth through critical weeks More active and more yellow - signs of better health
Chick Weight at Day 14* Chicks at Day 7*
290
270
250 Control
230
Grams
210
190
170
BioTyton 10^7
150
Saline 10^1 10^3 10^5 10^7 10^9
Control BioTyton doses (CFU/egg)
BioTyton is delivering the standard, targeted benefits of probioticA Business Unit of White Dog Labs
WDL’s Staff –
Leaders in Isolating, Analyzing,
Cultivating and Engineering Clostridia
HominBio has started applying to
human health, the Clostridia technology
WDL is currently applying to animal healtha-b-eBio Chemicals A Business Unit of White Dog Labs
MixoFerm™ Addresses
The Industrial Bio Challenge – Carbon Yield
Glucose has
• 6 carbon
• 6 oxygen
• Low energy density
• Carbon = $$$
• Oxygen = -$$$
Most chemicals have carbon backbone with little to no oxygen.
How does conventional fermentation discard the oxygen?Conventional Ethanol Fermentation
Glucose Ethanol Carbon Dioxide
Fermentation sacrifices 33% of the carbon to get rid of
oxygen and densify energy into ethanolMixoFerm – Ethanol Example
Glucose Ethanol Water
+
Hydrogen
6x
100% carbon yield with water as only by-productMixoFerm Acetone Example
Mass yield
(ton product per ton of sugar)
0.8
Increased product yield (50–100%)
0.7
for nearly any fermentation product
0.6
0.5
0.4
Acetone
0.3
CO2
0.2
0.1 Reduced fermentation
CO2 emissions
0
Conventional
MixoFerm - no gas
MixoFerm - H2 added
WDL has developed and demonstrated the MixoFerm process for acetoneNew Product Opportunities for Post Low Priced Oil
Conventional MixoFerm™ MixoFerm H2 added
Required amount of H2 reducing gas
Practically unlimited product opportunities when oil >$100 compared to mass of carbohydrate consumedCompany Presentation Outline
• Overview
• Company
• Executive team
• Board of directors
• Advisors
• Investors
• Locations
• Technology
• Products
• Company organization
• Animal Nutrition – ProTyton, MiruTyton
• Animal Health – BioTyton
• Human Nutrition and Health
• Biochemicals
• Production
• Ethanol plant repurposing
• NuMake-1 at MRE, NebraskaProTyton Manufacturing Features
Renewable Corn feedstock Anaerobic Fermentation Compatibility with ethanol plants
• Plentiful and relatively inexpensive feedstock • Highly scalable process • Straightforward re-purposing of ethanol plants
• Same as used in wine, beer and ethanol • Leveraging existing facilities & staff reduces capital
industries investment and time to market
• Low CapEx and OpExEthanol Boom & Bust Creating Plant Owners Fatigue
Return Per Bushel of Corn
$5.50
$4.50
Return to corn farmers
$3.50
Return to ethanol producers
$2.50
Neither farmers nor producers are
$1.50
doing well over past several years
$0.50
$0.50
$1.50
Jan-04 Oct-06 Jul-09 Apr-12 Dec-14 Sep-17
Conversion of ethanol to ProTyton production is a 3-way win:
Reduces ethanol capacity, increase ProTyton Capacity, increases corn consumptionAnnounced Planned ProTyton Production Capacity
In collaboration with MRE ethanol plant in Sutherland, NE
2020: 3,000 m-ton/year ProTyton, 16,000 s-ton MiruTyton
2021: 30,000 n-ton/year ProTyton, 160,000 s-ton MiruTytonYou can also read
