Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau

 
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Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
Collaboration between
 IRVT- OIE RL Brescia- Anses

Labib Bakkali, Stéphan Zientara, Pascal Boireau
                      ANSES
              Animal Health Laboratory
                  Maisons-Alfort
             Email: pascal.boireau@anses.fr
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
www.anses.fr

               Maisons-Alfort Animal Health
                       Laboratory
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
Animal Health Laboratory

                       Created in1901

                                                       Bull.soc.fr.hist.méd.sci.vét., 2006, 6 : 79-107

                           virulentes, premiers vaccins à base de                    porteurs sains, règles de la prophylaxie
                           d’aphtes ou de tissus de cobayes                          sanitaire (Vallée et Carré),    premier
                           neutralisés (Carré , Rinjard et Vallée : leurs
                           travaux ouvriront la voie au vaccin de
                           Schmidt et Waldmann ), hémo-prévention
                           (Rinjard), culture in vivo sur divers tissus
                           de fœtus de bovin (Thomas, Dhennin) ou
                           sur lapin nouveau-né (Verge, Dhennin,
                           Asso), vaccination par voie intradermique (
                           L.et L. Dhennin, Paraf, Larenaudie),
                           absence d’immunité croisée entre virus
                           OAC et SAT 1 (Santucci et coll.),
                           diagnostic      et     épidémiosurveillance
                           (Goureau, Kaiser, Moutou)

                            Fièvre charbonneuse : diagnostic,
                           surveillance, contrôle (Vaissaire).

                           Fièvre catarrhale ovine et Fièvre du Nil
                           occidental : surveillance de ces deux
Edmond Nocard (1900)       Emile Roux
                           maladies, diagnostic et surveillance de la                         Henri Carré
                           maladie, enquêtes sérologiques évaluation                        Figure 2 : Henri Carré dans son
                           des vaccins (Zientara)                                                     laboratoire
                           Germes pyogènes : production de sérums                    diagnostic de l’avortement à virus de la
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
REFERENCE MISSIONS
Agrement Agriculture ministry, Health ministry, DG SANCO, OIE, FAO

                   2 European Reference laboratories
                           Brucellosis – Equine diseases

               OIE reference laboratory - FAO Collaborative center
                          Brucellosis | Tuberculosis | EHDV

                              OIE Collaborative center
                                 Food borne parasites

                              Human reference center
                                  Brucellosis
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
REFERENCE MISSIONS
   Agrement Agriculture ministry

                    2 LABORATOIRES
         15 REFERENCE
         DE national mandates
                       COMMUNAUTAIRE
                  Brucellose – Maladies équines
  Anthrax,
  Chlamydiosis
  Brucellosis and tularemia
         LABORATOIRE DE RÉFÉFENCE OIE - CENTRE
  Tuberculosis
  FMD                COLLABORATEUR FAO
  BluetongueBrucellose
               and EHDV  | Tuberculose | Paratuberculose
  West Nile
  Swine vesicular disease
                     Centre national de référence
  Viral horse diseases  and glanders, dourine (with
                              Brucellose
  DOZULÉ Laboratory)
                 Centre national de référence associé
  Parasite transmitted by food Trichinellosis,
                         Charbon bactéridien
  Protozoa, Anisakis, (arthropod (ticks and
  phlebotomes) borne diseases (Bartonella,
  Anaplasma, Leishmania…))
  Schmallenberg virus, Bunyavirus
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
Campus Alfort

              New lab C3FMDV, tick
              borne diseases « I3 »

                         CRBM
FMDV C3 lab
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
Training and international cooperation on
                           FMDV

                       Training and technology transfert
                       Research project for the
                      development of new tools

Networks REMESA and
     RESOLAB
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
Bilateral actions on FMDV

                                    Maisons-Alfort
                                  18/10/10 to 29/10/10
                                  - 2 training weeks
                                  - Providing Reagents

 Marocco    Tunisia     Algéria
 Mustapha    Soufien     Nadera
HAMMOUCHI   SGHAIER    CHAOUACH
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
Workshop and training for FMDV diagnosis tests.

                   FAO                   Mauritanie
   Tchad
                 Bénin Niger Tunisie             Liban
                                                   Pakistan
      Algérie

       ANSES – Laboratoire de Santé Animale, Maisons-Alfort, France
                            21 – 25 May 2012
Collaboration between IRVT- OIE RL Brescia-Anses - Labib Bakkali, Stéphan Zientara, Pascal Boireau
Training seminar Accra
                  Vet laboratory ACCRA (Ghana)
                    17 – 21 SEPTEMBER 2012
Kees van Maanen                                  Labib Bakkali Kassimi

                                                                  CAMEROON,
                                                             Dr. Simon Dickmu Jumbo

                                                           CENTRAL AFRICA REPUBLIC
                                                             Dr. Marie-Noel Ouaimon

                                                                COTE D’IVOIRE
                                                              Dr. Gnabro H. P. Godji

                                                                  D. R.CONGO
                                                              Dr. Leopold Mulumba

                                                                     GHANA
                                                                 Dr. J. A. AWUNI

                                                                    NIGERIA
                                                             Dr. Hussaini G. Ularamu

                                                             REPUBLIC OF CONGO
                                                             Dr. Ngakeni Faramalala

                                                                   SENEGAL
                                                             Dr. Papa Serigne SECK

                                                                     TOGO
                                                             Mr. Felix Kodzo WOUME
Training seminar on FMDV ACCRA: practical traning

                                    Training for direct
                                    indirect tests

                     LFD

             Ag-ELISA & NSP ELISA

                   RT-PCR
Workshop on epidemiological surveillance of FMD and the
              capacity of the laboratory

                   Dakar, 3-4 June 2014
                   Mali, Mauritania and Senegal

                    Project of common
                    epidemiological surveillance
                    among the three countries
Molecular epidemiology

      Isolation and characterisation of FMDV strains

        PhD                       Ghana                 Nigeria                Cameroon
   Benin
    Type O                       Type O                SAT 2                    SAT 2

     Type A

         Gorna et al.
     Vet.Microbiol. 2014

→ University of Liege, Belgium     Accra Veterinary     FMD Research Centre,            Laboratoire
→ University of Abomey-Calavi        Laboratory,         National Veterinary             National
  in Cotonou, Benin                    Ghana             Research Institute,            Vétérinaire
→ Belgian Development                                          Nigeria                  Cameroun
  Collaboration Department
  Institute of Tropical
  Medicine (Antwerp)
                                               Collaborations
Bilateral actions on FMDV
                                     Maisons-Alfort
                              16 juin 2014 au 27 juin 2014
                                - 2 weeks for training on emerging
                                outbreak
                                - To provide reagents
                                - Practical training in Tabat between
                                29/9 and 3/10/2014

Nadia CHAFFAI   Amina AQARI

        Biopharma
       Rabat Marocco
Practical training in the field for FMDV

                                            Stephan
                                           ZIENTARA

     Nakuru, Kenya 25-29 August 2014
Support actions to REMESA for FMD

   Workshops and Training

  Bilateral action program of French
  ministry of agriculture:
     Welcome trainees
     Training in countries by experts
  Actions supported by EuFMD
     Organization of workshop at
       Maisons-Alfort
     Participation to real time training
Support actions to Tunisia for FMD

 Collaboration project between IRVT, OIE RL Brescia and Anses

            IRVT                  IZSLER

         Genetic and antigenic characterization of FMDV
                  strains circulating in Tunisia

Funded by:
French Ministry of Agriculture, Food and Forestry
Agency for the development of international cooperation in the areas of agriculture, food
and rural space (Adecia)
Instituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER)
Context

FMD was introduced in
 Tunisia in April 2014
First case was reported in
 North East of country
                                    29/4/2014            13/10/2014
Then, different outbreaks were
 reported in different region
 and in a short time

Virus identified : serotype O of               WRLFMD
 topotype Ind-2001d
Objective

Investigation of FMDV evolution during the outbreak

Use of genome sequence and antigenic profiling data
 to study:

      dynamic of FMDV transmission

      the virus matching with vaccine strains

         Identify risk factors for the spread of the
                   disease in the country
Planned activities

                        Action                          Month
IRVT will send samples to Anses                           1

Welcome trainee from IRVT at Anses for 3 weeks            2

Analysis of samples by trainee : virus isolation, RT-   2 et 3
PCR, typing
Genome amplification of selected samples at Anses       4 et 5

Send isolats and amplicons to IZSLER                      5

Full genome sequencing from amplicons by IZSLER         6à9

Antigen profiling of isolats by IZSLER                  6à9

Data analysis                                           10-11
Repport                                                  12
Output

 Complete sequences of isolates of FMDV in Tunisia
 Phylogenetic analysis of sequences
 Antigenic characterization of isolates
 Analysis of epidemiological links between isolates based
  on phylogenetic analyzes
 Analysis of antigenic homology between the vaccine
  strains and isolates
Conclusion

Global control of FMD needs coordinated action at
 national, regional and international level
Collaborations between veterinary services and
 laboratories at regional and international levels are
 essential for the success of the control of FMD
Anses participates and will continue to participate actively
 in the global fight against FMD
In particular Anses-Animal health Lab, continues to
 provide support to REMESA members through training,
 expertise and collaboration project
Collaboration projects such as that between IRVT, IZSLER
 and Anses should be encouraged
Thank you for your attention

      “Unity makes strength”
New diagnostic tools

  Improve the existing tests
Multiplex RT-PCR in one step for West Africa

advantages:
 Detection and typing of four serotypes in one reaction
 Internal amplification control
 Cost
Duplex RT-PCR in one step.

                                          advantages:
                                           Simple (faster)
                                           Less handling
                                           Less risk of contamination
                                           sensitive
IRES/β-actine
                                           Internal amplification control
     Or                                    Stability Mastermix
 3D/β-actine

                20µl   5µl
Alternative way for sample sendings

                  Inactivated
                     virus

Pen-Side test
                                                   advantages:
                                                    Less risk
                                                    Shipping Costs

                                  Extraction
                                    ARN

                         RT-PCR                Transfection

                                                  Virus
Research project

      Ensemble pour mieux comprendre

Identification and characterization of
circulating strains in West Africa
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