Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed

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Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
Organic Heterogeneous Material, what
is it and what are the potential benefits
          for the organic sector?

                         Matteo Petitti
                         Rete Semi Rurali
               LIVESEED International Workshop, 25th June 2020
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
Outline of today’s OHM session

    Organic Heterogeneous Material: what
      is it and where it comes from

    The European legal framework: a brief
            history of the process

      Practical examples of OHM in Italy
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
What is OHM? Practical examples
     (…)

    CCP         Increasing diversity   • Composite Cross Population

Open-
                                       • Dynamic mixtures/populations
Pollinated
variety
                                       • Farmers Selection (of
                                         landraces/populations)
Pure line, F1                               Important to have a wide range of
hybrid
                                            species & cultivar types with sufficient
                                            intra-cultivar diversity that are adapted
                                            to variable growing conditions and the
                                            demands of different value chains
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
Organic Heterogeneous Material:
  Exploiting natural evolutionary dynamics

 Harry Harlan - 1929                             Coit Suneson - 1956
“This inexpensive and easily executed method [...] being proposed has been neglected or
overlooked by many plant breeders focusing on techniques for improving man’s [sic]
selecting and testing efficiency, rather than on evolutionary fitness as determined by
survival” (Suneson, 1956)

“A pure-line mentality, convinced that variation was bad, uniformity was good and off-
types in the field somehow immoral, developed [...] Thus it was that we laid ourselves open
to epiphytotics of serious dimensions” (Harry Harland - cited by Martin Wolfe, 1985).
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
Organic Heterogeneous Material:
                            First deployment in Europe

Martin Wolfe’s as a plant pathologist discovered that barley populations sported much
higher mildew resistance than pure lines.
• Wolfe’s barley CCP was adopted at large scale in the former DDR, reaching >90% of
   malting barley cultivated area.
• Fungicides consumption for these crops dropped to almost zero

Wolfe, M. S. (1985). The current status and prospects of multiline cultivars and variety mixtures for disease resistance. Annual Review of Phytopathology, 23, 251–273. doi:10.1146/annurev.py.23.090185.001343.
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
Innovative seed systems for OHM
• For best results OHM seed should be produced in the
  same geographical region where it will be cultivated
• Decentralised seed enterprises for OHM can offer new
  opportunity to organic farmers
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
What are the advantages of OHM for
            the Organic Sector?
• Low cost evolutionary breeding methods
• Exploiting specific adaptation and Genotype x
  Environment Interactions (GxE)
• Climate change adaptation

                                                  www.co2.earth
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
OHM: a Novel Cultivar Type
                                      Organic plant reproductive material
                                     New Organic Regulation 2018/848/EU

                  Organic Heterogeneous Material
                                                               Organic varieties suitable for organic
                                    –
                                                                            production
                 High level of genetic and phenotypic
                                                                                 –
                                diversity
                                                                     Adjusted DUS and VCU
                                    –
                           No DUS and VCU                            → new temporary
Heterogeneous Cereal                                                 experiment
     Populations
         –                  → Delegated act
    2014/150/EU

                            Conventional seed production CAP

     • Definition of organic plant breeding
     • Definition of organic heterogeneous material for all crops
     • See Liveseed Milestone Report M2.8 ‘Main outcomes and SWOT of
       experiences from marketing populations under the Temporary
       Experiment into the commercialisation of heterogeneous populations in
       the European Union’ for an update on 2014/150/EU
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
Organic Heterogeneous Material:
                The legal framework
                                                             The PRM “Better Regulation”

• First mention of “Heterogeneous Material” as a new category of seed
• After rejection of the Commission’s proposal by the EU parliament, the Commission
  sets up a “Temporary Experiment” for cereal population marketing
Organic Heterogeneous Material, what is it and what are the potential benefits for the organic sector? - Matteo Petitti - Liveseed
Organic Heterogeneous Material:
                   The legal framework
Extension of 2014/150/EU to 2021

 New Organic Regualtion 484/2018                   New Organic Regulation starts

                                      201

                                                                                       202
                                         8

                                                                                          1
                                                                                                           2021
                                                                                              Organic Heterogeneous Material
                                                                                                     coming into force
                               201

                                                                           201
                                  4

                                                                              9-20
             2014/150/EU
                                             Delegated Acts Negotiation

                                                                                  20
                       • Participating Member States:
                          • Denmark
                          • France
                          • Germany
                          • Italy
                          • The Netherlands
                          • UK
Organic Heterogeneous Material:
                    The legal framework
 Extension of 2014/150/EU to 2021

  New Organic Regualtion 484/2018                   New Organic Regulation starts

                                       201

                                                                                        202
                                          8

                                                                                           1
                                                                                                            2021
                                                                                               Organic Heterogeneous Material
                                                                                                      coming into force
                                201

                                                                            201
                                   4

                                                                               9-20
              2014/150/EU
                                              Delegated Acts Negotiation

                                                                                   20
• 2018: Organic Heterogenous Material is introduces as a
  new category of seed in the New Organic Regulation (art.
  3 & 13) approved by the European Parliament
• 2019: Hungary joins the ”Temporary Experiment”
Organic Heterogeneous Material:
                     The legal framework
  Extension of 2014/150/EU to 2021

   New Organic Regualtion 484/2018                   New Organic Regulation starts

                                        201

                                                                                         202
                                           8

                                                                                            1
                                                                                                             2021
                                                                                                Organic Heterogeneous Material
                                                                                                       coming into force
                                 201

                                                                             201
                                    4

                                                                                9-20
               2014/150/EU
                                               Delegated Acts Negotiation

                                                                                    20
• The results of the “Temporary Experiment” have been
  informing the Commission in drafting the Delegated Acts
  regulating OHM seed registration and certification
• Negotiation between Commission and all actors involved
  (IFOAM-EU, ECO-PB, Arche Noah & Seed Savers groups
New Organic Regulation
      Organic Heterogeneous Material
New organic regulation (EU) 2018/848 put into force January 2021
Article 3 Definitions
• (18) ‘organic heterogeneous material’ means a plant grouping within a
   single botanical taxon of the lowest known rank which:
• (a) presents common phenotypic characteristics;
• (b) is characterised by a high level of genetic and phenotypic diversity
   between individual reproductive units, so that that plant grouping is
   represented by the material as a whole, and not by a small number of
   units;
• (c) is not a variety within the meaning of Article 5(2) of Council
   Regulation (EC) No 2100/94 ( 1 );
• (d) is not a mixture of varieties; and
• (e) has been produced in accordance with this Regulation
Political Framework
      Organic Heterogeneous Material
New organic regulation (EU) 2018/848 put into force January 2021
Article 13 Specific provisions fort he marketing of plant reproductive
material of organic heterogeneous material
1. Plant reproductive material of organic heterogeneous material may be
   marketed without complying with the requirements for registration and
   without complying with the certification categories of pre-basic, basic
   and certified material or with the requirements for other categories,
   which are set out in Directives 66/401/EEC, 66/402/EEC, 68/193/EEC,
   98/56/EC, 2002/53/EC, 2002/54/EC, 2002/55/EC, 2002/56/EC,
   2002/57/EC, 2008/72/EC and 2008/90/EC or acts adopted pursuant to
   those Directives.
New Organic Regulation
       Organic Heterogeneous Material
New organic regulation (EU) 2018/848 put into force January 2021
Article 13
2. Plant reproductive material of organic heterogeneous material as referred to in
paragraph 1 may be marketed following a notification of the organic
heterogeneous material by the supplier to the responsible official bodies referred
to in Directives …. made by means of a dossier containing:
  (a) the contact details of the applicant;
  (b) the species and denomination of the organic heterogeneous material;
  (c) the description of the main agronomic and phenotypic characteristics that are
  common to that plant grouping, including breeding methods, any available results
  from tests on those characteristics, the country of production and the parental
  material used;
  (d) a declaration by the applicant concerning the truth of the elements in points
  (a), (b) and (c); and
  (e) a representative sample.
New Organic Regulation
         Organic Heterogeneous Material
New organic regulation (EU) 2018/848 put into force January 2021
Article 13
2. After having expressly or implicitly acknowledged the notification, the responsible official
body may proceed to the listing of the notified organic heterogeneous material. That listing
shall be free of charge to the supplier.
The listing of any organic heterogeneous material shall be communicated to the competent
authorities of the other Member States and to the Commission

3. The Commission is empowered to adopt delegated acts in accordance with Article 54
supplementing this Regulation by setting out rules governing the production and marketing
of plant reproductive material of organic heterogeneous material of particular genera or
species, as regards:
(a) the description of the organic heterogeneous material, including the relevant breeding
and production methods and parental material used;
(b) the minimum quality requirements for seeds lots, including identity, specific purity,
germination rates and sanitary quality;
(c) labelling and packaging;
(d) information and samples of production to be kept by the professional operators;
(e) where applicable, maintenance of the organic heterogeneous material.
Organic Heterogeneous Material:
                       The legal framework
    Extension of 2014/150/EU to 2021

     New Organic Regualtion 484/2018                   New Organic Regulation starts

                                          201

                                                                                           202
                                             8

                                                                                              1
                                                                                                               2021
                                                                                                  Organic Heterogeneous Material
                                                                                                         coming into force
                                   201

                                                                               201
                                      4

                                                                                  9-20
                 2014/150/EU
                                                 Delegated Acts Negotiation

                                                                                      20
LIVESEED dissemination at EU Commission level:
• May 2018 - DG Sante Brussels
• December 2018 - CPVO Angers
• May 2019 - CREA-DC Bologna, Italy
• October 2019 - CPVO Angers, (arable crops)
• December 2019 - CPVO Angers, (vegetable crops & examination officers AM)
• January 2020 - DG Sante, DG Agri, DG Envi, LIVESEED/ECOBREED/BREZOV
  meeting in Brussels
• February 2020 – DG Sante, DG Agri, LIVESEED/INVITE joined workshop in
  Brussels
Organic Heterogeneous Material
Recommendations from LIVESEED
Organic Heterogeneous Material
     Recommendations from LIVESEED

Figure 2. Checklist in successive steps of the general provisions for registration of
“Organic Heterogeneous Material”.
Organic Heterogeneous Material
        Recommendations from LIVESEED
• All crop species can be OHM
• Production of OHM means development (human and/or natural
  selection) under certified organic conditions for at least 3 / 5 years.
• Evidence and traceability is provided via organic certification
• Breeding methods should comply with organic principles (at least
  respect to natural crossing barriers)
• Simple notification procedure in collaboration with national
  authorities
• No intellectual property rights
• Production of plant reproductive material (PRM) (seed
  multiplication) of OHM should also be conducted under certified
  organic agriculture
• Seed health and quality parameters should be defined (germination,
  disease etc.)
• No quantity restriction
• Truthful labelling throughout, i.e. seller is s liable
Heterogeneous Population Description

 Liveseed Milestone Report M2.8 ‘Main outcomes and SWOT of
 experiences from marketing populations under the Temporary Experiment
 into the commercialisation of heterogeneous populations in the
 European Union’ for an update on 2014/602/EU
Organic Heterogeneous Material Description
                           Farmers’ selection        Dynamic population        CCP
                           Highly relevant           Highly relevant List of   Highly relevant List of
   Origin
                           Original population       parents                   parents
                                                     Medium relevance as       Medium relevance as
                           Highly relevant in
                                                     related to natural        related to natural
   Region of cultivation   terms of e.g.
                                                     selection during          selection during
                           geographical origin
                                                     development               development
                                                     Low relevance as the
                                                                               Highly relevant for
   Breeding method 1:                                starting point is
                           Not relevant                                        the methods used in
   constitution                                      always a physical
                                                                               the initial crosses
                                                     mixture
                                                     Medium relevance if       Medium relevance if
                           High Relevance to         direct selection is       direct selection is
   Breeding method 2:
                           describe which            applied, otherwise        applied, otherwise
   development (pre-
                           selection has been        related to region of      related to region of
   notification)
                           applied                   cultivation specifying    cultivation specifying
                                                     management                management
                           Medium relevance,
   Breeding method 3:                                Medium relevance          Low relevance as
                           and it may change
   production /                                      and might change if       progeny is supposed
                           significantly according
   multiplication (post-                             self- or cross-           to be multiplied as
                           to the species life
   notification)                                     pollinated species        bulk
                           cycle
                           High relevance as         Medium relevance if       Medium relevance if
                           related to selection as   direct selection is       direct selection is
   Phenotypic traits
                           advised by the            applied as advised by     applied as advised by
                           ‘breeder’                 the ‘breeder’             the ‘breeder’
                                                     Medium relevance as       High relevance as also
   Traceability            Low relevance             also keeps track of       keeps track of natural
                                                     natural selection         selection
Italian cereal populations
Registered Italian Populations as of 31 December 2018

ØSoft (bread) wheat: 8
ØDurum wheat: 3
ØBarley: 1
vEmilia-Romagna: Bioadapt, Bio2, BioAppennino
vToscana: Solibam tenero Floriddia, Mix tenero Toscana Pa1, Mix
tenero Toscana 1, Evoldur (durum), Mix duro Toscana Pa1
vUmbria: Barley Mix 48
vMolise: SOLIBAM duro Petacciato
vBasilicata: Carosella, SOLIBAM duro Recchia
vSicilia: Solibam tenero Li Rosi, Angelo (durum)
Case 1: Barley CCP ‘Mix 48’
                                                   Orzo «MIX48 popolazione»
University of Perugia                                   PER AGRICOLTURA A BASSI INPUT
                                DIPARTIMENTO DI SCIENZE AGRARIE, ALIMENTARI E AMBIENTALI (dsa3)

• 2018: 1.1 tons (2 farms)                    UNIVERSITÀ DEGLI STUDI DI PERUGIA

                               •     DA MIGLIORAMENTO GENETICO EVOLUTIVO
• 2019: 2.75 tons (6 farms)    •
                               •
                                     ELEVATO LIVELLO DI DIVERSITÀ INTRASPECIFICA
                                     MESCOLANZA DI LINEE DISTICHE E POLISTICHE
                               •     BASSO IMPIEGO DI FATTORI DI PRODUZIONE
                               •     ELEVATA PRODUZIONE E STABILITÀ DELLA PRODUZIONE*

                                              Caratteristiche principali
                                ! Epoca di spigatura media                   maggio          CONSIGLI GESTIONE AGRONOMICA
                                "Altezza media                               72.5 cm         ! Areale di coltivazione      Centro e Sud Italia
                                # Produzione media (50 unità azoto/ettaro)   3.86 t/ettaro
                                                                                             "Epoca di semina              Fine ottobre-novembre
                                $Resistenza allettamento media               buona
                                %PESO 1000 semi medio                        34 g            # Densità di semina           200 kg/ettaro
                                & Proteine totali                            11.2 %ss        $Concimazione                 50 unità azoto/ettaro
                                ' Setacciatura >2.8 mm                       38.4%           %Gestione erbe infestanti     NESSUN INTERVENTO
                                                                                             & Trattamenti fito-sanitari   NESSUN INTERVENTO

                               PER LO SVILUPPO DELLA MIX48 SONO STATI INIZIALMENTE INCROCIATI GENOTIPI PARENTALI
                                      CON ORIGINI E CARATTERISTICHE MORFO-FISIOLOGICHE DIVERSE (TABELLA).
                               LA POPOLAZIONE È STATA COLTIVATA IN CONDIZIONI DI BASSI INPUT E SOGGETTA ALLA SOLA
                                  SELEZIONE NATURALE PER 9 ANNI POI SOTTOPOSTA A 3 CICLI DI SELEZIONE MIRATI AD
                              INCREMENTARE LA SUA PRODUZIONE MANTENENDO ELEVATA VARIABILITÀ PER CARATTERI DI
                                                            INTERESSE AGRONOMICO.

                              *La popolazione MIX48 è stata sottoposta a prove agronomiche in ambienti diversi ed in anni
                              successivi assieme a diverse varietà commerciali. Le prove sono state condotte in biologico e
                              in regimi di bassi input. La popolazione è risultata caratterizzata da produttività pari a quella
                                     dei migliori controlli e da elevata stabilità produttiva, sia statica che dinamica.

                                   PER INFORMAZIONI: 075 5856218 valeria.negri@unipg.it - 075 5856212 lorenzo.raggi@unipg.it
EVOLUTIONARY BREEDING PROGRAM            Genetic diversity of EBP materials

                                                                                             Initial population
                                                                         00                 (AUT DBA & mix48)
                                                                                              genetic diversity

                                                                                             mix48 evolution
                                                                                            under contrasting
                                                                                           conditions (PG-IT, RI-
                                                                                             IT, HU, UK & SY)
                                                                                             genetic changes

                                Each color refers to a particular genetic component of the initially crossed parents
Agronomic evaluation of EBP materials: YIELD
•     Multi-environmental trials (4 years, 3 different locations)
•     2 populations : AUT DBA and mix48
•     13 EBP pure lines: selected from the EBP populations
•     9 controls: commercial varieties & recently developed lines selected through conventional
      breeding programs

     EBP populations and EBP pure lines characterised by
                         HIGH YIELD
             Controls characterized by lower yield
         in “low productivity” environments (P≤0.05)

                                            environments                                                     environments

Raggi, et al., 2017. Evolutionary breeding for sustainable agriculture: Selection and multi-environmental evaluation of barley populations and lines. Field Crops Research
Case 2: SOLIBAM Bread Wheat
  The original evolutionary
  population (EP) was created
  in 2009 at ICARDA, Syria
  mixing 1996 crosses (F2, F3
  and F4). In Italy it has been
  cultivated in 2 organic farms
  continuously since 2010-
  2011 evolving in 2 distinct
  EPs: SOLIBAM Floriddia in
  Tuscany and SOLIBAM Li
  Rosi in Sicily.
See: Petitti, et al. EUCARPIA Symposium on Breeding for Diversification &
DIVERSIFOOD Final Congress proceeding
https://orgprints.org/34951/
http://www.diversifood.eu/events/diversifood-final-congress/
Case 2: SOLIBAM Bread Wheat
•   200 parental lines
•   1996 crosses
•   Constituted in 2009
•   Seed shared among farmers…
Case 2: SOLIBAM Bread Wheat
…until certified seed became
                                                                                                                                                                                                                                                                                                                                                                                  ROSATI - ABRUZZO

                                                                                                                                                                                                                                                                                                                                                                                BADDE MENDULA - SARDINIA

                                                                                                                                                                                                                                                                                                                                                                                 COOP GERREI - SARDINIA
                                                                                                                                                                                                                                                                                                                                                                20ha
                                                                                                                                                                                                                                                                                                                                                                                 TRABALLOSU - SARDINIA

                                                                                                                                                                                                                                                                                                                                                                                  ARCHIMISSA - SARDINIA

commercially available in
                                                                                                                                                                                                                                                                                                                                                                                   DEIANA - SARDINIA
                                                                                                                                                                                                                                                                                                                                     ROSATI - ABRUZZO
                                                                                                                                                                                                                                                                                                                                                                                    DESOGUS - SARDINIA

                                                                                                                                                                                                                                                                                                                                                                                    FADDA - SARDINIA

                                                                                                                                                                                                                                                                        ROSATI - ABRUZZO                                                                                            KENTOS - SARDINIA

                                                                                                                                                                                                                                                                                                                                        PEPE - ABRUZZO
                                                                                                                                                                                                                                                                                                                                                                                 TERRA & ABBA - SARDINIA

2017 thanks to 2014/150/EU
                                                                                                                                                                                                                                                                         PEPE - ABRUZZO
                                                                                                                                                                                                                                                                                                                                                                                    ROCCATANI - SARDINIA

                                                                                                                                                                                                                                                                                                                                     BATTAGLIA - ABRUZZO                             RICCI - SARDINIA
                                                                                                                                                                                                                                                                        VALENTE - ABRUZZO
                                                                                                                                                                                                                   ROSATI - ABRUZZO
                                                                                                                                                                                                                                                      5KG                                                                                                                          CARBONI - SARDINIA
                                                                                                                                                                                                                                                5 KG                                                                                       SARDINIA 3
                                                                                                                                                                                                                                                                                                                                                                                      MICELI - SICILY
                                                                                                                                                                                                                                                                    BATTAGLIA - ABRUZZO
                                                                                                                                                                                                                                                                                                                                                                                      MELI -SICILY
                                                                                                                                                                                                                                                                                                                          GIORGIO (+ VL SARDA) - SARDINIA
                                                                                                                                                     ROSATI - ABRUZZO                                                                                                                                                                                         400 KG
                                                                                                                                                                                                                                                                                                                                                                                     MILITELLO - SICILY
                                                                                                                                                                                                                                                                                                                                                              150 KG
                                                                                                                                                                                                     15KG                                                               SARDINIA 3                                                 CABONI - SARDINIA          150 KG              LA VIA DEL GRANO - SICILY
                                                                                                                                                                                                                                                                                                                                                              100 KG
                                                                                                                                                                                                                                                                                                                                                              100 KG
                                                                                                                                                                                                                                                                                                                                                                                     NICOLETTI - SICILY
                                                                                                                                                                                                                        SARDINIA 3                                                                                                                            100 KG
                                                                                                                                                                                                                                                                  GIORGIO (+ VL SARDA) - SARDINIA
                                                                                                                                                                                                                                                                                                                                   BRUNETTI - CALABRIA        100 KG
                                                                                                                                                                                                                                                                                                                                                                                     TERRA DEL SOLE - SICILY
                                                                                                                                                                                                                                                                                                                                                              150 KG
                                                                                                                                                                                                                        SARDINIA                                           CABONI - SARDINIA                                                                   75 KG                  STASSI - SICILY
                                                                                                                                                                                                                                                                                                                               AURORA
                                                                                                                                                                                                                                                                                                                                                                100 KG
                                                                                                                                                                                                                     CABONI - SARDINIA                                                                                                                         125 KG                CARDACI - SICILY
                                                                                                                                                                                                                                                                        BRUNETTI - CALABRIA
                                                                                                                                                                                                                                                                                                                                     COCCI                    100 KG
                                                                                                                                                                                                                                                                                                                                                              750   KG
                                                                                                                                                                                                                                                                                                                                                                750 KG
                                                                                                                                                                                                                                                                                                                                                                                      CUCCIA - SICLILY
                                                                                                                                                                                                                    BRUNETTI - CALABRIA
                                                                                                                                                                                                                                                                                                                                                              1250 KG
                                                                                                                                                                                                                                                                                                                               DE ANGELIS - MARCHE
                                                                                                                                                                                                                                                                                                                                                               2100 KG            CASA VECCHIA - EMILIA ROMAGNA
                                                                                                                                                                                                                                                                                                                                                               100 KG
                                                                                                                                                                                                                     DE ANGELIS - MARCHE
                                                                                                                                                                                                                                                                                                                                  BRUNI - MARCHE               500 KG                  LE MURA - UMBRIA
                                                                                                                                                                                                                                                                       DE ANGELIS - MARCHE                                                                      2050 KG
                                                                                                                                                                                                                     BRUNI - MARCHE
                                                                                                                                                                                                                                                                                                                                                              1500 KG
                                                                                                                                                                                                                                                                                                                                 ANNIBALI - MARCHE                                     PETICCA - LAZIO
                                                                                                                                                                                                                                                                                                                                                               1100 KG
                                                                                                                                                                                                                                                                         BRUNI - MARCHE                                                                        200 KG
                                                                                                                                                                                                                    ANNIBALI - MARCHE                                                                                                DI BUO' MARCHE                                    CACCHI - LAZIO
                                                                                                                                                                                                                                                                                                                                                                   50 KG
                                                                                                                                                                                               20KG
                                                                                                                                                                                                                                                                        ANNIBALI - MARCHE                                                                        300 KG                VITTORI - CAMPANIA
                                                                                                                                                                                              20KG                                                                                                                               VITTORI - MARCHE
                                                                                                                                                                                                                                                                                                                                                                 100 KG
                                                                                                                                                                                                                     DI BUO' MARCHE
                                                                                                                                                                                               20KG                                                                                                                                                              200 KG                GUARINI - PUGLIA
                                                                                                                                                                                                                                                                         DI BUO' MARCHE                                           MARIANI - MARCHE                400 KG
                                                                                                                                                                                               440KG
                                                                                                                                                                                                                                                                                                                                                                   200 KG

                                                                                                                                                           LI ROSI - SICILY                                       LI ROSI - SICILY                                                                                                                                               LI ROSI - SICILY
                                 LI ROSI - SICILY               20 KG         LI ROSI - SICILY                73 KG      LI ROSI - SICILY                                                                                                                           LI ROSI - SICILY                                           LI ROSI - SICILY
                                                                                                                                                                                                                                                      15KG
                                                                                                                                                                                                                                                                                                                                                                                     DE ANGELIS - MARCHE
                                                                                                                                                                                                                                                       2KG
                                                                                                                                                                                                                                                                        SAN MARINO                                                  SAN MARINO
                                                                                                                                                                                                 20KG                                                                                                                                                                                   DI BUO' MARCHE
                                                                                                                                                                                                                                                         30KG
                                                                                                                                                                                                                                                                 CASA DELLE AGRICOLTURE - PUGLIA                            CASA DELLE AGRICOLTURE - PUGLIA                               SAN MARINO

                                                                                                                                                                                                                                                                      RECCHIA - BASILICATA                                                                                    CASA DELLE AGRICOLTURE - PUGLIA
                                             3KG                                                                                                                                                                             MISCELLA                                                                                              RECCHIA - BASILICATA

                                                                                 PAZZAGLI - TUSCANY                                                                                                                                                                           MISCELLA                                                                                                  RECCHIA - BASILICATA

                                                                                                                                                                                                                      15KG                                                                                                          CARINI - MARCHE
                                                                                                                                                                                                                                                                          CARINI - MARCHE                                                                                               IL SALTO - MARCHE
                                SOLIBAM SOFT                                                                                                                                                                              CARINI - MARCHE
                                                                                                                                                                                                                                                                                                                                                                                      A. MONALDI - MARCHE

                               WHEAT 2011-2017
                                                                                                                                                                                                                                                                                                                                    ROSSI- LOMBARDIA
                                                                                                                                                           STERPAIA - TUSCANY                                                   15KG
                                                                                                                                                                                                                                                                         STERPAIA - TUSCANY
                                                                                   SSSUP - TUSCANY                                                                                                                                                                                                                                                                                      CIOMMI - MARCHE

                                   ICARDA
                                                                                                                                                                                                                                                                                                                                    TIBI - PIEMONTE
                                                                                                                                                                                                                         ROSATI - ABRUZZO
                                                                                                                                                                                                                                                                                                                                   ROSSI F. - MARCHE                                   GIROBELLI - MARCHE

                               EVOLUTIONARY                                                                                                                                                           1KG
                                                                                                                                                                                                                        BERNA - UMBRIA
                                                                                                                                                                                                                                                                                                                                 PONTILLO - CAMPANIA                                     CARINI - MARCHE

                                 POPULATION
                                                                                                                                                                                                                                                                    VERONESI - EMILIA ROMAGNA
                                                                                VALLI UNITE - PIEMONTE                                                                                                                                                                                                      9KG
                                                                                                                        VALLI UNITE - PIEMONTE                                                                      DINELLI - EMILIA ROMAGNA
                                                                                                                                                                                                        1KG                                                                                                  1KG                PELLEGRINO - CAMPANIA                                      TIBI - PIEMONTE
                                                                  4KG
                                                                                                                                                                                                                                                                         MODEGGI - TUSCANY
                                                                                                                                                                                                            1KG        BELLINI - TUSCANY                                                                      1KG
                                                                                                                                                                                                                                                                                                                                  PANDOLFI - TUSCANY                                     ROSSI F. - MARCHE
                                                                                                                                                                                                                                                                                                              1KG
                                                                   4KG                                                                                                                               1.5KG                                             2KG
                                                                                                                                                                                                                                                                                                               1KG
                                                                                                                                                                                                                                                         100KG                                                                     PACETI - TUSCANY                                   PONTILLO - CAMPANIA
                                          2KG                                                                                                                                                                                                                                                                     3KG
                                                                                                                                                                                                            1KG
                                                                                                                                                                                                                                                                                                                   1KG
                                                                                                                                                                                                                                                                                                                                                                                      PELLEGRINO - CAMPANIA
                               FLORDIDDIA - TUSCANY                      FLORIDDIA - TUSCANY                          FLORIDDIA - TUSCANY              FLORIDDIA - TUSCANY                              FLORDIDDIA - TUSCANY                                    FLORIDDIA - TUSCANY                                     FLORIDDIA - TUSCANY
                         3KG

                                                                                                               4KG
                                                                                                                                                                                                                                                                                                                                                                                 FLORIDDIA - TUSCANY
                                                                                                                                                                                                                                                                                                                                                                    100 KG
                                                                                                                                                                                                                                                  5KG
                                                                                                                         AGRICOLA NUOVA - LAZIO                                                                                                                                                                                                                    100 KG
                                                                                                                                                                                                                                                                    RIVIERE - RANCIA                                                                               100 KG                 BRUNETTI - CALABRIA
                                                                                                              3KG
                                                                                                                                                                                                                                                                                                                                                                  100 KG
                                                                                                                                                                                                                                  500GR
                                                                                                             2KG
                                                                                                                                                                                                                                                                                                                                                                   100 KG                  FELICIOTTI - TUSCANY
                                                                                                                                                                                                                                                                                                     1KG                                                             100 KG

                                                                                                                                                                                                                                  500GR
                                                                                                                                                                                                                                                                                                                                                                                           ANTONINI - TUSCANY
                                                                                                                                                                                                                                                                                                                                                                     100 KG
                                                                                                                                                                                                                                                                  CARUCCI - PIEMONTE                   1KG                                                         100 KG
                                  DI BENEDETTO - PUGLIA 23KG                                                                    TAVAZZANI                                                                                                                                                             1KG
                                                                                                                                                                                                                                                                                                                                                                   100 KG                     FAZZI - TUSCANY
                                                                                                                                                                                                                                                1KG                                                                      VENTURELLI - CAMPANIA                    100 KG
                                                                                                                                                                                              1KG                                                                   CERRANO - TUSCANY               9.6KG
                                                                                                                                                                                                                                                                                                                                                                   100 KG
                                                                                                                                                                                                                                                                                                                                                                                           DI GRIGOLI - TUSCANY
                                                                                                                                                                                                                                                                                                                                                                   100 KG
                                                                                                                         PISANI - PIEMONTE                                                                                                                                                           1KG                      ANGELINI - LAZIO
                                                                                                                                                                                                                                                                                                                                                                   100 KG
                                                                                                                                                                                                                               500GR                                                                1KG                                                                                     CORROTTI - TUSCANY
                                                                                                                                                                                                                   CENACCHI - EMILIA ROMAGNA                                                                                                                     100 KKG
                                                                                                                                                                                                                                                                                                                           BERTONI - TUSCANY                      100 KG
                                                                                                                                                                                                                                                  2 KG                                                                                                                                       BALZANI - TUSCANY
                                                                                                                                                                                                                                                                                                                                                                  100 KG
                                                                                                                                                                                                                             BELLIN - TUSCANY                                                                            CASCINA GALLINA - LOMBARDIA              100 KG
                                                                                                                                                                                                                                                                                                                                                                                            DI STEFANO - TUSCANY
                                                                                                                                                                                                                                                                     MARMUCCINI - TUSCANY                                                                          100 KG
                                                                                                                                                                                                                                                                                                                             LASTRUCCI - TUSCANY                   100 KG
                                                                                                                                                                                                                                                                                                                                                                                            PANDOLFINI - TUSCANY
                                                                                                                                                                                                                                                                                                                                                                   100 KG
                                                                                                                                                                                                                                                                    SAN BONIFACIO - VENETO
                                                                                                                                                                                                                                                                                                                                                                   100 KG
                                                                                                                                                                                                                                                                                                                         MATIAZZI - EMILIA ROMAGNA                                           PASTORE - TUSCANY
                                                                                                                                                                                Via PAolo di Luzio                                                                CENACCHI - EMILIA ROMAGNA
                                                                                                                                                                                                                                                                                                                                                                                            FRANCHINI - TUSCANY

                                                                                                                                                                                                                                                                     BELLIN - TUSCANY                                                                                                         CARUBIA - TUSCANY

                                                                                                                                                                                                                                                                                                                                                                                               MEACCI - TUSCANI
                                                                                                                                                                                                                                                                     BATTEZZATO - MOLISE                                      MARMUCCINI - TUSCANY
                        YEAR OF                                                                                                                                                                                                                                                                                                                                                            MARGIACCHI - TUSCANY
                        HARVEST:                                                                                                                                                                                                                                                                                               SAN BONIFACIO - VENETO
                                                                                                                                                                                                                                                                                                                                                                                           RONCALEGGI - TUSCANY
                     STARTING WITH                                                                                                                                                                                                                                    DESBRI - LOMBARDIA
                                                                                                                                                                                                                                                                                                                            CENACCHI - EMILIA ROMAGNA                                        BRUSCHI - TUSCANY
                    FIRST SOWING IN
                                                                                                                                                                                                                                                                                                                                  BELLIN - TUSCANY                                       MOLINO ANGELI - TUSCANI
                      OCTOBER 2010
                                                                            SELECTION OF 2012 BEST EARS                                                                                                                                                                                                                                                                                       PASSERINI - TUSCANY
                                                                                                                                                                                                                                                                                                                                  PETACCIATO - MOLISE
                                                                                                                                                                                                                                                                                                                                                                                                 PARISI - UMBRIA

                                                                                                                                                                                                                                                                                                                                  DESBRI - LOMBARDIA                                           BELLIN - TUSCANY

                                                                                                                                                                                                                                                                                                                                                                                                MUSINI - UMBRIA

                                                           NO SELECTION                                                                                                                                                                                                                                                                                                                    VENTURELLI - CAMPANIA

                                                                                                                                                                                                                                                                                                                                                                                               ANGELINI - LAZIO

                                                                                                                                                                                                                                                                                                                                                                                             BERTONI - TUSCANY
                                                         FARMER SELECTION

                                                                                                                                                                                                                                                                                                                                                                                        CASCINA GALLINA - LOMBARDIA

                                                                                                                                                                                                                                                                                                                                                                                           SAN BONIFACIO - VENETO

                                                                                                                                                                                                                                                                                                                                                                                       CENACCHI - EMILIA ROMAGNA (?)

                                                                                                                                                                                                                                                                                                                                                                                            PETACCIATO - MOLISE

                                                                                                                                                                                                                                                                                                                                                                                            DESBRI - LOMBARDIA

                                                                                                           FLORIDDIA                               FLORIDDIA                                FLORIDDIA                                                                                                                     FLORIDDIA
                                                                                                                                                                                                                                                   FLORIDDIA
                                                                                                          SELECTION -                             SELECTION -                              SELECTION -                                                                                                                   SELECTION -
                                                                                                                                                                                                                                                  SELECTION -
                                                                                                            TUSCANY                                 TUSCANY                                  TUSCANY                                                                                                                       TUSCANY                                                         FLORIDDIA
                                                                                                                                                                                                                                                    TUSCANY
                                                                                                                                                                                                                                                                                                                                                                                          SELECTION -
                                                                                                                                                                                                                                                                                                                                                                                            TUSCANY

                                          2011                              2012                                   2013                            2014                                      2015                                                            2016                                                           2017                                                           2018
SOLIBAM bread wheat seed sales

• 2017   2 tonnes
• 2018   2.4 tonnes
• 2019   6 tonnes
                       SOLIBAM FLORIDDIA (SOL_FL)

• 2017   11.4 tonnes
• 2018   15.2 tonnes
• 2019   23.7 tonnes
                       SOLIBAM LI ROSI (SOL_LR)
Climate, OHM and Specific Adaptation

                   Tuscany
                   Sicily
Climate, OHM and Specific Adaptation

                    SOLIBAM FLORIDDIA (SOL_FL)

                    SOLIBAM LI ROSI (SOL_LR)
LIVESEED tomato – RSR & Arcoiris
         Starting material: tomato CCP obtained by crossing four local
          varieties selected               forMay
                                (Deadline 16th   suitability
                                                     2014)            under organic management
         Solibam WP 3 deliverable (D3.4): Individual partner contributions DRAFT 1

                      CdB8-Allongée       CdB1-        CdB4-Cuor di    CdB3-Cœur de
                        d'Alicante      Muchamiel          bue             Bœuf

           Country         Spain           Spain           Italy           France
            Cycle          Long          Medium            Short          Medium
          Strength           3               4               1                3
 Plant   Fruit Load          5               4               3                2
           Growth     Indeterminatrd   Determinated   Indeterminatrd   Indeterminatrd
            Other      Regular truss
            Shape       Rectangular      CdBP c4         CdBP c1           CdBC
          Average
                         150-190         190-250         190-250          190-250
           Weight
 Fruit      Green
                           Yes             Yes             Yes              Yes
          shoulder
         Coloration      Red             Light red      Light red        Light red
            Other     Homogeneity        Firmness         Shape            Shape

2013 study on the parents in Brittany

              6000
LIVESEED tomato – CREA-OF
• MAGIC tomato population derived from
  eight parental lines (ISI sementi)
• Participatory selection in 3 Organic farms
Courgette population – CREA-OF
• Cross Composite Population created by Gabreile Camapelli of CREA-OF (Monsampolo del Tronto, Italy)
• Grown as a population with success by organic farmers in the Marche region
Courgette population – CREA-OF
• Participatory selection of lines within the populations in an organic farm
• Well adapted to organic and no-tillage system

                                                                               Dark green fruit selection

                                                                               Light green fruit selection
Dwarf bean population – CREA-OF
• Population (dynmaic mixture) of common bean (dwarf) created by mixing 70 cultivars
• Selection within the dwarf bean population according to seed size

                                          2016   S1

  2018   S4 large seed (sub population)                2018   S4 small seed (sub popolation)
Chickpea population – CREA-OF
• Chickpea Population (Dynamic mixture created by mixing 45 cultivars)
MAIZE
• In partnership with
  CREA-CI Bergamo
  (2016 to 2020)
• Participatory
  evaluation of 17
  local vatieties and
  resulting half-dialled
  crosses (171 F2 and
  F3)
• Costitution of RSR
  MAIS CCP using 17
  crosses chosen by
  farmers
OATS

Initiative of CREA-CI Bergamo
in collaboration with RSR
(2019-20)
• Hulled oat CCP (36
    parental lines)
• Hulless oat CCP (17
    parental lines)
In the pipeline…
• LENTILS in
  cooperation with
  Scuola Superiore
  Sant’Anna of Pisa.
  Starting from 63 local
  varieties of CNR
  genebank of Bari
• RICE in cooperation
  with CARIPLO
  foundation. Starting
  from 246 Italian
  accessions from CREA
  Vercelli and IRRI
LIVESEED publications on OHM
                PRACTICE ABSTRACT No. 53

         Tools to identify heterogeneous cereals 1:
                         constitution
                                 Problems
Identification and traceability of genetically diverse populations is required for variety registration and seed
certification. Whilst in (genetically homogeneous) varieties one individual plant can represent the whole plant
grouping, making univocal identification possible, in (genetically heterogeneous) populations an individual plant
cannot represent the population, and therefore a range of description and identification metrics is needed.

  • D 2.8 Proposal Solutionsfor a toolbox for identification and description of organic
      heterogeneous material
A temporary experiment on the marketing of genetically diverse populations of wheat, barley, oats and maize was
granted under the EU implementing decision 2014/150/EU. It assessed different tools’ effectiveness at
  • M 2.8 population
guaranteeing   Main outcomes           and
                           identification andSWOT      of experiences
                                               the identity of the seeds of afrom     marketing
                                                                               population.           populations
                                                                                            It was possible to conduct a
SWOTunder
       analysisthe   Temporary
                of the different toolsExperiment       into
                                       used in different      the commercialization
                                                          contexts.                             of heterogeneous
                                                                    The first set of tools that were  critiqued related to
breeding goals, varieties used in the crossing, breeding schemes and production methods – or the constitution of
      populations in the European Union
registered populations (Table 1).
      Various
  •Table         Practice
         1: Summary           Abstracts
                       of the SWOT    analysisby  LIVESEED
                                                conducted        partners,
                                                            on tools relating toavailable      on project
                                                                                 the constitution             website
                                                                                                    of populations
      LIVESEED Booklet #1, Part Three
  •STRENGTHS                                                          WEAKNESSES
 ● Can give an overview of the intended purpose of the population                      ● A breeding goal may not translate into reality
 ● Can provide full and transparent information on the origin and genetic history of   ● Breeding goals may not provide sufficient detail on end-use, often
THANK YOU FOR YOUR ATTENTION
  matteo.petitti@semirurali.net
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