OWLS 2012 - Italy-BioImaging
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OWLS 2012
ORGANISING COMMITTEE
Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy
Francesco Baldini, National Research Council, Florence, Italy
Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy
Francesca Cella Zanacchi, Istituto Italiano di Tecnologia, Genova, Italy
Valeria Delle Cave,Istituto Italiano di Tecnologia, Genova, Italy
Zeno Lavagnino, Istituto Italiano di Tecnologia and University of Genoa, Italy
Mattia Pesce, Istituto Italiano di Tecnologia, Genova, Italy
Manuela Rigano, University of Genova, Italy
Manuela Salvatori, Istituto Italiano di Tecnologia, Genova, Italy
Federico Tixi, Istituto Italiano di Tecnologia, Genova, Italy
Cesare Usai, National Research Council, Genova, Italy
PROGRAM
Alberto Diaspro, Istituto Italiano di Tecnologia and University of Genoa, Italy
Silvio Aime, University of Turin, Italy
Francesco Baldini, National Research Council, Florence, Italy
Gert von Bally, University of Muenster, Germany
Paolo Bianchini, Istituto Italiano di Tecnologia, Genoa, Italy
Francesca Cagnoni, Istituto Italiano di Tecnologia, Genova, Italy
Halina Rubenstein-Dunlop, University of Queensland, Australia
Hans-Jochen Foth, Technical University of Kaiserslauter, Germany
Min Gu, Swinburne University, Australia
Partha Pratim Mondal, Indian Institute of Science, Bangalore, India
Stephen Morgan, University of Nottingham, UK
Colin JR Sheppard, National University of Singapore, Singapore
Cesare Usai, National Research Council, Genova, Italy
OWLS • Genoa, 4-6 July 2012 • Program • Page 1Exhibit Area 2nd Floor G Nikon Instruments Spa H IIT - Istituto Italiano di Tecnologia / LANIR.EU I Physik Instrumente (PI) S.r.l. L HAMAMATSU PHOTONICS ITALIA M Till Photonics GmbH N Fianium Limited Page 2 • Program • OWLS • Genoa, 4-6 July 201 2
3rd Floor
A OLYMPUS ITALIA SPA
B VUTARA Inc.
C TOPTICA Photonics AG
D Bitplane AG
E-F Leica Microsystems Srl
OWLS • Genoa, 4-6 July 2012 • Program • Page 3Page 4 • Program • OWLS • Genoa, 4-6 July 201 2
OWLS 2012
Tuesday, July 3rd - 7.00pm
Inaugural Ceremony
Sala del Maggior Consiglio, Palazzo Ducale
www.palazzoducale.genova.it
Alberto Diaspro, OWLS President
Roberto Cingolani, Scientific Director, Istituto Italiano di Tecnologia
“Lettura Magistrale”
Scott E. Fraser, Caltech, CA
Imaging the Molecular and Cellular Dynamics of Embryogenesis
Welcome Cocktail kindly offered by
Camera di Commercio di Genova
Leica Microsystems
Bitplane
OWLS • Genoa, 4-6 July 2012 • Program • Page 5Wednesday, July 4th
9.00 - 9.30 Magazzini del Cotone - Congress Center
http://www.portoantico.it
Scirocco-Libeccio Room
OWLS 2012 Opening Lecture
Karsten Konig, Saarbrucken University, Germany
Clinical Multimodal Hybrid Multiphoton/CARS Tomography of In Vivo Human Skin
Chair: A. Diaspro
Parallel Sessions
9.30 - 10.00 Scirocco - Libeccio Room Ponente Room
David Sampson Gertrude Bunt
University of Western Australia, AustraliaUniversity of Goettingen, Germany
Optical elastography - high-resolution Visualization of multiple coupled signal-
imaging of tissue mechanical properties. ing events in a single cell by fluorescence
Chair: G. Scarcelli lifetime pattern analysis.
Chair: R. Bizzarri
10.00 - 10.45 Optical application to biomedicine Advanced imaging techniques I
Chair: G.Scarcelli Chair: R. Bizzarri
Andrea Curatolo Barbara Storti
University of Western Australia, Australia NEST, Pisa Italy
Portable 3D optical coherence elastogra- Multiplexing correlation spectroscopy
phy for applying microscopic imaging of and resonance energy transfer unveils dy-
tissue mechanical properties in the clinic. namical interactions between transient
George A. Stanciu receptor potential vanilloid 1 (trpv1) and
University Politehnica Bucharest, Romania microtubules at nanoscale in living cells.
Electrical charge on the hydroxyapatite Dominika O. Trembecka-Lucas
surface investigated by laser scanning Jagiellonian Universit. Poland
microscopy. Interaction between HP1 dimer and
S. Beke PCNA in DNA replication and repair, as
Istituto Italiano di Tecnologia (IIT), Gen- revealed by bimolecular fluorescence
ova, Italy complementation assay (BiFC), and
Excimer laser-based stereolithography FRET/FLIM.
for rapid fabrication of rigid biodegrad- Simona Rodighiero
able photopolymer scaffolds for tissue Fondazione Filarete, Milan, Italy
engineering. FRET study of 4.1R, ICln and actin inter-
actions: a link between cell morphology
and cell proliferation.
Page 6 • Program • OWLS • Genoa, 4-6 July 201 210.45 - 11.15 Giuliano Scarcelli Ranieri Bizzarri
Harvard Medical School, USA National Research Council, Italy
Brillouin microscopy to study the Novel probes and strategies for high-res-
bio-mechanical properties of cornea and olution fluorescence imaging in living
lens. cells.
Chair: D.Sampson Chair: G.Bunt
11.15 - 11.45 Coffee break
11.45 - 12.15 Scirocco-Libeccio Room
Monika Ritsch-Marte
Medizinische Universität Innsbruck, Austria
Applications of spatial light modulators in biophotonics: from optical tweezers to
advanced microscopy.
Chair: F.Difato
Parallel Sessions
12.45 - 13.30 Scirocco - Libeccio Room Ponente Room
Plasmonics Advanced imaging techniques II
Chair: E. Di Fabrizio Chair: A. Periasamy
Emanuela Esposito Antonella Battisti
CNR-ICIB Napoli, Italy NEST, Pisa, Italy
Lab-on-Fiber Technology: Nanophoton- Intracellular pH measurements made
ics Within Optical Fibers. simple by genetically-encoded fluo-
Fang Liu rescent probes and phasor approach to
Tsinghua University, Beijing FLIM.
Integrated Bio-Sensor based on Hybrid Ottavia Golfetto
Coupler with Surface Plasmon Polariton University of California, Irvine, California
and Dielectric Waveguide. Laurdan fluorescence lifetime as a bi-
Ranjeet Kumar osensor for membrane fluidity.
Indian Institute of Technology New Delhi, Deo R. Singh
India University of Wisconsin Milwaukee, USA
Clustering of Au-NPs near plasmon ex- Quaternary structure of wild type and
citation and enhanced scattering based mutated rhodopsin at room and body
in-vitro detection of Ehrlich ascites car- temperature using spectral FRET.
cinoma cells.
13.30 - 15.30 Lunch, posters, exhibition, Job corner.
Company Presentations
14.00 - 15.00 Scirocco - Libeccio Room Ponente Room
Olga Levai Maria Marosvölgyi
Leica Microsystems Till Photonics
Opening the gate to Super-Resolution. Combining advanced fluorescence imag-
ing techniques on the same living sample:
spinning disk confocal, TIRF, FRAP(PA)
and FRET.
OWLS • Genoa, 4-6 July 2012 • Program • Page 715.30 - 16.00 Scirocco - Libeccio Room
Anthony Watts Oxford University, UK
Realizing bio-imaging potential: Enhancing bacteriorhodopsin-mediated
molecular scale, wavelength-selective photoswitching.
Chair: A. Periasamy
Parallel Sessions
16.00 - 16.30 Scirocco - Libeccio Room Ponente Room
Ilaria Testa Arthur Chiou
Max Planck Institute Goettingen, Germany National Yang-Ming University, Taiwan
RESOLFT Nanoscopy in Life Sciences: (R.O.C.)
unraveling the mysteries with low light Optical Tweezers Based BioMicrorheology
levels Chair: D. Cojoc
Chair: G. Vicidomini
16.30 - 17.15 Single molecule and super-resolution Optical Tweezers
Chair: I.Testa Chair: A. Chiou
Paolo Annibale Francesco Difato
EPFL, Lausanne, Switzerland Istituto Italiano di Tecnologia, Genova,
Towards accurate dual color PALM im- Italy
aging. Position and Force-clamp interferometric
Francesca Cella Zanacchi tracking of an optically trapped probe to
Istituto Italiano di Tecnologia, Genova, study cytoskeleton dynamics of devel-
Italy oping mouse neuron.
Super-resolution microscopy of large Pasquale Bianco
biological samples within a light sheet Università degli Studi di Firenze, Italy
based architecture. The energy landscape of DNA over-
Davide Mazza stretching transition determined with a
Fondazione S.Raffaele, Milano, Italy Dual Laser Optical Tweezers under force
Measurement of binding in living cells by clamp.
single molecule tracking. A benchmark Dan Cojoc
for protein/scaffold interactions. Materials Technology Institute, Trieste,
Italy
Local delivery of small numbers of attrac-
tant and repulsive guidance molecules to
neuronal cells by optical manipulation
techniques.
17.15 - 17.45 Mike Heilemann Maria Luisa Calvo Padilla
Wuerzburg University, Germany Universidad Complutense de Madrid, Spain
Quantitative Single Molecule Biology Holography and its applications.
with Photoswitchable Fluorophores. Chair: A.Chiou
Chair: I.Testa
Page 8 • Program • OWLS • Genoa, 4-6 July 201 217:45 - 18:15 Coffee break
18:15 - 18:45 Scirocco - Libeccio Room
Brad Amos Cambridge, UK
A laser-scanning microscope for optical sectioning of large-volume specimens such
as mouse embryos using a Mesolens.
Chair: C.J.R. Sheppard
Parallel Sessions
18.45 - 19.30 Scirocco - Libeccio Room Ponente Room
Optical tomography Optical application to biomedicine II
Chair: C.J.R. Sheppard Chair: K. Braeckmans
Stephen P Morgan Wei Zhang
University Park, Nottingham, UK Tsinghua University, Beijing, China
Pulsed ultrasound modulated optical to- Trace level multi-gas sensing in mid-in-
mography of tissue frared hollow-core Bragg fiber supporting
Maria Pogorelova multi-wavelength transmission
Moscow State University, Moscow, Russian Tadelech
Federation Laser-Induced Breakdown and X-Ray
Optical microtomography of early mouse Fluorescence Spectroscopic Techniques
embryo with laser scanning microscopy in Detection of Elements in Tea Leaves
Ludovico Silvestri Tewfik Soulimane
University of Florence, Italy University of Limerick, Ireland
Whole-brain neuroanatomy on the mi- Photosterilisable Textiles for Improved
cron-scale with confocal light sheet mi- Hospital Hygiene
croscopy
19.30-20.00 Martin J. Booth Dayong Jin
Oxford University, UK Macquarie University, Sydney, NSW 2109,
Adaptive optics for high resolution bio- Australia
logical microscopy Novel coding and decoding in suspension
Chair: C.J.R. Sheppard arrays for accelerated biomolecular dis-
covery and personalized medicine.
Shan Shan Kou
EPFL, Lausanne, CH-1015 Switzerland
Three-dimensional (3D) Monte Carlo
Simulation of Born’s Diffractions for Cel-
lular Analysis
OWLS • Genoa, 4-6 July 2012 • Program • Page 9Thursday, July 5th
8.30 - 9.00 Scirocco - Libeccio Room
RMS Lecture
Tony Wilson Oxford University, UK
Making Light Work in Microscopy.
Chair: F. Brakenhoff
Parallel Sessions
9.00 - 9.30 Scirocco - Libeccio Room Ponente Room
Martin Oheim Amitabha Chattopadhyay
Université Paris Descartes, France Centre for Cellular and Molecular Biology
Non-evanescent excitation light in ob- Hyderabad, India
jective-type TIRF microscopy limits to Organization and Dynamics of the Se-
quantitative imaging and super-resolu- rotonin-1A Receptor in Live Cells using
tion microscopy. Fluorescence Microscopy.
Chair: J. Bewersdorf Chair: S. Maiti
9.30 - 10.15 STED microscopy I Advanced Imaging Techniques III
Chair: J. Bewersdorf Chair: A. Chattopadhyay
Giuseppe Vicidomini Cosimo Arnesano
Istituto Italiano di Tecnologia, Genoa, Italy University of California Irvine, Irvine, Cal-
Towards Low-Intensity STED Microscopy ifornia
by Time Gating. Digital Parallel Frequency-Domain Spec-
Lorena Benedetti troscopy for Tissue Imaging.
University of Milan, Italy Stefano Coppola
Morphological features of 4.1R overex- Sapienza - University of Rome, Italy
pressing cells characterized with Correla- Intracellular trafficking of multicompo-
tive Light-Scanning Electron Microscopy nent lipoplexes: a fluorescence micros-
and observed with gated-STED nanoscopy. copy study.
Tilman Rosales Anna Letizia Allegra Mascaro
National Institutes of Health, USA University of Florence, Italy
Instrumentation for super-resolution us- In vivo imaging of climbing fibers reactive
ing new low power probes. plasticity after laser axotomy.
10.15 - 10.45 Joerg Bewersdorf Sudipta Maiti
Yale University, USA Tata Institute of Fundamental Research,
New Approaches in STED Microscopy: India
TIRF and Phase Retrievaly. Protein misfolding versus aggregation:
Chair: M. Oheim following the early events with an ultra-
sensitive fluorescence tool.
Chair: A. Chattopadhyay
Page 10 • Program • OWLS • Genoa, 4-6 July 201 2 OWLS • Genoa, 4-6 July 2012 • Program • Page 1010:45 - 11:15 Coffee break
11.15 - 11.45 Scirocco - Libeccio Room
Ernst H.K. Stelzer
Goethe University Frankfurt , Germany
From the ultramikroskop to theta microscopy to tetrahedral microscopy to light
sheet-based fluorescence microscopy.
Chair: F. Cella Zanacchi
Parallel Sessions
11.45 - 12.30 Scirocco - Libeccio Room Ponente Room
Single Particle Tracking Label Free Techniques
Chair: Z. Lavagnino Chair: P. O’Shea
Hendrik Deschout Francesca Romana Bertani
Ghent University, Belgium National Research Council, Rome, Italy
On-chip sheet illumination for sin- Hyperspectroscopic confocal reflectance
gle-particle tracking measurements in imaging of cultured cells.
bio-fluids. Alessandro Candiani
Katrien Forier University of Parma, Italy
Ghent University, Belgium Label-free DNA sensor based on optical
Single particle tracking microscopy: To- fiber grating devices.
wards nanomedicines for the treatment Dan Cojoc
of chronic pulmonary infections in cystic National Research Council, Trieste, Italy
fibrosis patients. Secondary speckle sensing microscopy
Christelle Rosazza for fast detection of malaria.
University of Konstanz, Germany
Single Particle Tracking of DNA in cells
delivered by electroporation.
12.30 - 13.00 Kevin Braeckmans Paul O’ Shea
University of Ghent, Belgium University of Nottingham, UK
Sizing Nanomatter in Biological Fluids by Imaging specific proteins in cells;
Fluorescence Single Particle Tracking. time-resolved dynamics and disposition
Chair: Z. Lavagnino without GFP.
Chair: A. Chattopadhyay
13.00 - 15.00 Lunch, posters, exhibition, Job corner.
Company Presentations
14.00-15.00 Scirocco-Libeccio Room Ponente Room
Luciano Lucas Alessandro Cometta
Bitplane Zeiss
Imaris: the 3D/4D image analysis soft- A super-resolution close-up view: from
ware SIM to PALM
Page 11 • Program • OWLS • Genoa, 4-6 July 201 2 OWLS • Genoa, 4-6 July 2012 • Program • Page 1115.00 - 15.30 Scirocco-Libeccio Room
Colin Sheppard
National University of SIngapore, Singapore and Istituto Italiano di Tecnologia, Italy
Imaging in the phase contrast microscope.
Chair: P.Saggau
15.30 - 16.00 Gail McConnell
University of Strathclyde, UK
Promising new wavelengths for multiphoton laser scanning microscopy: thinking
outside the Ti:Sapphire
Chair: P.Saggau
Parallel Sessions
16.00 - 17.30 Scirocco - Libeccio Room Ponente Room
Non Linear/Multiphoton Microscopy Fluorescent Probes/Sensors
Chair: G. McConnell Chair: F. Brandi
Giulia Borile Komal Bagga
University of Padova, Italy Istituto Italiano di Tecnologia, Genoa, Italy
Multiphoton fluorescence Ca2+ imaging Laser-based generation of luminescent
in acute cardiac slices. bio-nanomarkers for cell imaging.
Albrecht Haase Ilaria De Santo
University of Trento,Italy Istituto Italiano di Tecnologia, Naples, Italy
In-vivo morpho-functional imaging of Understanding Drug Localization in Dox-
the honeybee brain . orubicin Loaded TiO2 Nanotube Arrays
B. Leshem through Fluorescence Correlation Spec-
Paris Descartes University troscopy analysis.
Temporally focused illumination is inher- Joanna L Richens
ently robust against scattering. University of Nottingham, UK
Leonardo Sacconi Plasmonic-Based Biosensors in Disease
National Research Council, Florence, Italy Diagnosis.
Optical recording of intracellular action Shu-Han Wu
potential propagation in cardiac myo- National Yang-Ming University, Taipei,
cytes. Taiwan
Godofredo Bautista Dynamic Study of Cell Secretion using
Tampere University of Technology, Fin- Microfluidic Traps and Gold Nanoslits
land Based Surface Plasmon Sensors.
Wide field third-harmonic generation Milena Stephan
microscopy of turbid media. Georg-August University of Goettingen,
Neeven A. Hosny Germany
Imperial College London, Great Britain Combining Fluorescence Microscopy
Viscosity imaging of lipid-based micro- with Reflectometry: Development of a
bubbles using fluorescence lifetime im- Biomembrane Sensor.
aging (FLIM) of molecular rotors.
Page 12 • Program • OWLS • Genoa, 4-6 July 201 2 OWLS • Genoa, 4-6 July 2012 • Program • Page 1217.30 - 18.00 Scirocco - Libeccio Room
Francesco S. Pavone
European Laboratory for Non Linear Spectroscopy, University of Florence
In Vivo Reactive Neural Plasticity Investigation by means of Correlative Light-Elec-
tron Microscopy.
Chair: P. Bianchini
18.00 - 19.15 OWLS Assembly with Coffee break
20.30 Social Dinner
Villa dello Zerbino
http://www.villalozerbino.it/
OWLS • Genoa, 4-6 July 2012 • Program • Page 13Friday, July 6th
9.00 - 9.30 Scirocco - Libeccio Room
Enrico Gratton
University of California Irvine, US
Super-resolution by feedback imaging: mechanisms of translocation through the
nuclear pore complex.
Chair: P. Bianchini
9.30 - 10.00 Daniel Farkas
Cedars-Sinai Medical Center, USA
Multimode Optical Molecular Imaging of Disease for Better Diagnosis and
Treatment.
Chair: P. Bianchini
Parallel Sessions
10.00 - 10.45 Scirocco - Libeccio Room Ponente Room
Amyloid Studies Optics in the environmental science
Chair: C. Canale Chair: F. Baldini
Valeria Musi Malgorzata Biczysko
Ecole Polytechnique Fédérale de Lausanne, Istituto Italiano di Tecnologia, Pisa, Italy
Switzerland Simulation of optical spectra line-shapes
Bloch surface wave optical platform for for biomolecules in natural environmen-
sensing the aggregation kinetics of amy- tal conditions.
loid beta peptides. Towards the early de-
Sanjaya Thilakarathne
tection of Alzheimer’s disease. Saitama University, Japan
Bidyut Sarkar Ultra short term growth dynamics of
Tata Institute of Fundamental Research, Soybean and Radish under ozone stress
Mumbai, India using Statistical Interferometry Tech-
Label free dopamine imaging with multi- nique - a comparison with conventional
photon ultraviolet microscopy. ozone assessment measures.
Niels Zijlstra Dayong Jin
University of Twente, Netherlands Macquarie University, Sydney, Australia
Establishing the composition of al- Tuning the Rare-earth Nanoparticles for
pha-synuclein amyloid oligomers using Background-free Biosensing.
single-molecule photobleaching.
10.45 - 11.15 Coffee break
11.15 - 11.45 Scirocco - Libeccio Room
Nicolai Urban
Max Planck Institute Goettingen, Germany
Dynamic superresolution microscopy deep inside living brain tissue.
Chair: P. Bianchini
Page 14 • Program • OWLS • Genoa, 4-6 July 201 2Parallel Sessions
11.45 - 12.30 Scirocco - Libeccio Room Ponente Room
Sted Microscopy II Novel Techniques
Chair: B. Harke Chair: E. Gratton
Paolo Bianchini Cristophe Silien
Istituto Italiano di Tecnologia, Genoa, Italy University of Limerick, England
Single wavelength 2PE-STED nanoscopy. Chemical Imaging with Super Resolution
Brian R. Rankin Infra-Red Absorption.
Mobius Photonics, CA, USA P. Nandakumar
A new laser for STED BITS Pilani K. K. Birla Goa Campus, India
Jay R. Knutson Laser scanning photothermal microscopy.
National Institutes of Health, USA William M. Whelan
A new class of low-power probes for mul- Atlantic Veterinary College, Canada
ticolor nanoscopy. Frequency spectrum analysis in pho-
toacoustic tissue characterization.
12.30 - 13.00 Peter Saggau Ari T. Friberg
Baylor College, Texas, USA National Institute of Advanced Industrial
Advanced Optical Imaging for Re- Science and Technology (AIST), Japan
verse-Engineering the Brain Classical Ghost Imaging of Phase Objects
Chair: B. Harke and through Phase Aberrations
Martijn Stopel
University of Twente, Netherlands
Room temperature excitation spectros-
copy of single quantum dots
13.00 - 15.00 Lunch, posters, exhibition, Job corner.
15.00 - 15.30 Scirocco - Libeccio Room Ponente Room
Maria Garcia Parajo
ICFO- Institut de Ciences Fotonique Barcelona, Spain
Cell surface nanoscopy using single molecule optical nanotools
Chair: M. Heilemann
15.30 - 16.00 EBSA Lecture
Andreas Zumbusch
Universitat Konstanz, Germany
Coherent anti-Stokes Raman Scattering microscopy of intracellular dynamics
Chair: M. Heilemann
OWLS • Genoa, 4-6 July 2012 • Program • Page 15Parallel Sessions
16.00 - 17.30 Scirocco - Libeccio Room Ponente Room
Nanoparticles & Smart Materials Force Microscopy
Chair: M. Lorenzoni Chair: C. Canale
Athanassia Athanassiou Marco Capitanio
Istituto Italiano di Tecnologia, Genoa, Italy European Laboratory for Non-linear Spec-
Nanocomposite surfaces with photoin- troscopy, Sesto Fiorentino, Italy
duced wettability gradient for directional Ultrafast force-clamp spectroscopy of
growth of cells. single biomolecules
Francesco Baldini Josè L.Toca-Herrera
National Research Council, Floresnce, Italy University of Natural resources and Life
PMMA-nanoparticles for molecular bea- Sciences Wien, Austria
con delivery in cells for survivin mRNA Delivering mechanical and interfacial
detection. sample properties by combining optical
Despina Fragouli and atomic force microcopies
Istituto Italiano di Tecnologia, Lecce, Italy Jenu Varghese Chacko
Rendering cellulose sheets antibacterial Istituto Italiano di Tecnologia, Genoa, Italy
using silver nanoparticles formed by a AFM STED: Targeted probing with high
laser ablation technique. spatial precision
Alemu Kebede Raman Spectroscopy
Addis Ababa University, Etiopia Chair: R. Proietti
Controlled Synthesis, Characterization Riccardo Cicchi
and Application of Iron Oxide Nanopar- European Laboratory for Non-linear Spec-
ticles for Oral Delivery of Insulin. troscopy, Sesto Fiorentino, Italy
Enrico Rampazzo A combined fluorescence-Raman spec-
Università di Bologna, Italy troscopic probe for the diagnosis of
Fluorescent Silica-Core/PEG-Shell Nano- melanocytic lesions
particles with Tunable emission for Imag- Anna Chiara De Luca
ing and Labeling. Institute of Protein Biochemistry, Naples,
Laura D’Alfonso Italy
Università degli Studi di Milano Bicocca, Modulated Raman spectroscopy: a
Milan, Italy promising biophotonics tool for early
Intracellular diffusion of Gold Branched cancer diagnosis
Nanoparticles. Nicholas Smith
Osaka University, Japan
Raman imaging and analysis: from quan-
tification of cellular morphology to RNA
structure and intramolecular interac-
tions.
Page 16 • Program • OWLS • Genoa, 4-6 July 201 217.30 - 18.00 Closing Ceremony
Scirocco-Libeccio Room
OWLS 2012 Closing Lecture
Satoshi Kawata
Osaka University, Japan
Plasmons, Raman, nano and 3D, all in together.
Chair: A. Diaspro
18.00 - 18.30 Poster prizes
Sponsored by OWLS, Université Laval Québec, Camera di Commercio
di Genova
Farewell with Focaccia and white wine
Sponsored by Camera di Commercio, Leica Microsystems, Bitplane
Friday, July 6 2012 8.30pm
City Lights by LAMBS
www.lambs.it
Piazza De Ferrari, Genova (De Ferrari Square)
Saturday, July 7 2012 Full Day
Cinque Terre Tour
www.cinqueterre.it
OWLS • Genoa, 4-6 July 2012 • Program • Page 17OWLS 2012
Poster Session
“An insight of NoBoDys PerFect” Exhibition
P1 - P6 Page
P1 Gaser N. Abdelrasoul1,2 , Raffaella Magrassi3, Marco Scotto d’Abbusco1, Alberto 120
Diaspro1, 2
1 Department of Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy
2 Department of Physics University of Genova, via Balbi 5, 16126 Genova, Italy
3 National Research Council (CNR) - Institute of Marine Sciences (ISMAR), Genova, Italy
2PE Imaging of Cancer Cells Using PEGylated Gold Nanorods as Biocompatible Con-
trast Agent.
P2 Giulio Caracciolo1, Daniela Pozzi1, Anna Laura Capriotti2, Chiara Cavaliere2, 123
Francesco Cardarelli3, Angelo Bifone3, Giuseppe Bardi3, Fabrizio Salomone3 and
Aldo Laganà2
1 Department of Molecular Medicine, ‘Sapienza’ University of Rome, Viale Regina Elena, 324, 00161,
Rome, Italy
2 Department of Chemistry, ‘Sapienza’ University of Rome, P.le A. Moro 5, 00185, Rome, Italy,
3 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa, Italy
Protein corona controls the uptake delivery mechanisms of lipid gene vectors in can-
cer cell
P3 Tatjana Dramićanin, Lea Lenhardt, Ivana Zeković, Nasta Tanić and Miroslav D. 128
Dramićanin
Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia
Breast Cancer Detection by Support Vector Machine Classification of Synchronous
Fluorescence Spectroscopy Data
P4 Ivana Zekovića, Lea Lehnardta*, Tatjana Dramićanina, Miroslav D. Dramićanina 144
a Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia
Application of Supervised Self-Organizing Maps in Skin Melanoma Diagnosis by Total
Synchronous Fluorescence Spectroscopy
P5 Elisabetta M. Caspani, Philip H. Crossley, Carolina Redondo-Garcia, Salvador *
Martinez
Laboratory of Experimental Embryology, Instituto de Neurociencias UMH-CSIC, Alicante, Spain
Malignopodium: a new player in glioma progression
P6 Roberto Cighettia, Matteo Piazzaa, Valentina Calabresea, Gaetana Damorea 124
and Francesco Peria
a Università degli Studi di Milano-Bicocca, Piazza della Scienza, 2 – 20126 – Milano, Italy
New fluorescent molecular tools to investigate LPS recognition
Page 18 • Program • OWLS • Genoa, 4-6 July 201 2P16 - P30 Page
P16 Hanna Beckera,2, Amy Gladfelter3, Rudolf Oldenbourg1 121
1 Marine Biological Laboratory, Woods Hole, Massachusetts, USA
2 Universität Konstanz, Konstanz, Germany
3 Dartmouth College, Hanover, New Hampshire, USA
Quantitative and high resolution imaging of fluorescence anisotropy is shedding
light on septin architecture in the yeast bud neck
P17 Iván, Coto Hernández1,2, Giuseppe, Vicidomini1, Alberto, Diaspro1,2 125
1 Istituto Italiano di Tecnologia, Genova, Italia
2 Universita Degli Studi di Genova, Genova, Italia
Generation Of Super Continuum Source And Its Application In Nanoscopy
P18 William F. Falco, Joelson Fernandes, Evaristo A. Falcão, Anderson R.L. Caires 130
Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil
Effects on Photosynthetic Activity of Plants Induced by Silver Nanoparticles: Moni-
toring by Chlorophyll Fluorescence
P19 Joelson Fernandes1, William F. Falco1, Samuel L. Oliveira2, Anderson R.L. 131
Caires1
1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil
2 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS,
Brazil
Changes on Photosynthetic Activity of Herbicide-tolerant Soybean Plants Induced
by Glyphosate
P20 Jenni Karttunen1, Sari Mäntynen1, Heli Lehtivuori1, Janne Ihalainen1, Teemu 138
Ihalainen1, Jaana K. H. Bamford1 and Hanna M. Oksanen2
1 Department of Biological and Environmental Science and Nanoscience Center, PO Box 35, 40014
University of Jyväskylä, Jyväskylä, Finland
2 Institute of Biotechnology and Department of Biosciences, PO Box 56, 00014 University of Hel-
sinki, Helsinki, Finland
Protein Localization and Interactions in Bacteria Studied with Confocal Microscopy
and Fluorescence Lifetime
P21 Volkan KILIÇ, Gözde AYDOĞAN KILIÇ, Ahmet ÖZATA 140
Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY
Light microscopic pathology in Common carp ( Cyprinus carpio L.,1758) from Sa-
karya River (TURKEY)
P22 Lea Lenhardt1*, Ivana Zeković1, Tatjana Dramićanin1, Živoslav Tešić2, Dušanka 143
Milojković-Opsenica2, Miroslav D. Dramićanin1
1 Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001 Belgrade, Serbia
2 Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
Assessment of Fluorescence and Infrared Spectroscopy Capabilities for the Determi-
nation of Botanical Origin of Serbian Unifloral Honey
OWLS • Genoa, 4-6 July 2012 • Program • Page 19P16 - P30 Page
P23 Daniela Pozzi1, Giulio Caracciolo1, Francesco Cardarelli2, Angelo Bifone2, Heinz 152
Amenitsch3
1 Department of Molecular Medicine, “Sapienza” University of Rome, Viale Regina Elena, 324,
00161, Rome, Italy
2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa, Italy
3 Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Schmiedel-
strasse 6, A-8042 Graz, Austria
Confocal microscopy makes visible cholesterol-mediated gene delivery mechanisms
P24 Liliia Rohoza, Tatyana Dyubko, Sergey Galchenko, Boris Sandomirsky 153
Institute for Problems of Cryobiology and Cryomedicine fo the NAS of Ukraine, Kharkiv
Spectrofluorimetric investigation of interactions of heart peptides with blood serum
albumin
P25 Sebastian Tannert1, Peter Kapusta1, Alexander Glatz1, Christian Litwinski1, Uwe 154
Ortmann1, Rainer Erdmann1, Katrin Hoffmann2, Ute Resch-Genger2
1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, www.picoquant.com
2 Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Str. 11, 12489
Berlin, Germany)
On the spectral sensitivity calibration of fluorescence spectrometers: Extension to
the NIR, polarization and grating effects
P26 Anatoliy Tatarets1*, Yevgeniy Povrozin1, Olena Obukhova1, Iryna Fedyunya- 155
yeva1, Ewald Terpetschnig2, Leonid Patsenker1,2
1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001
Kharkiv (Ukraine)
2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,
Novel Squaraine Probes for Proteins and Fluorescence Imaging Applications
P27 Francesco Urciuolo1, Giorgia Imparato1, Costantino Casale1, Fabio Formiggini1, 156
Paolo Netti1,2
1 Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia, Largo Bar-
santi e Matteucci n, 53 80125 Napoli
2 Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, P. Le
Tecchio 80, 80125 Naples, Italy
Coupling Engineered Living Tissue with Multiphoton Tomography : towards in vitro
understating of biological phenomena
Page 20 • Program • OWLS • Genoa, 4-6 July 201 2P31 - P45 Page
P31 William Dallari, Marco Allione, Elena Samoylova, Marco Scotto d’Abbusco, 126
Alberto Diaspro
Nanophysics, Istituto Italiano di Tecnologia, via Morego, 30, 16163 Genova
Permanent and rewritable 3D optical data storage through multi-photon absorption
P32 Silvia Galiani1, Benjamin Harke1, Giuseppe Vicidomini1, Gabriele Lignani2, Fabio 133
Benfenati2, Hanako Tsushima2, Evelina Chieregatti2, Paolo Bianchini1, Alberto
Diaspro1
1Nanophysics, Istituto Italiano di Tecnologia, Genoa, Italy
2Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genoa, Italy
Optimization of a STED nanoscope
P33 Manuel Gunkela, Nina Beila, Jürgen Benekea, Slawek Mazura, Jürgen Rey- 135
manna, Bastian Schumachera and Holger Erflea
a Viroquant-CellNetworks RNAi Screening Facility, BioQuant, University of Heidelberg, Germany
Automated microscopy in RNAi screening experiments
P34 Timo Hakkarainen1, Tero Setälä1, Ari T. Friberg1,2,3 136
1 Department of Applied Physics, Aalto University, Box 13500, FI-00076 Aalto, Finland
2 Department of Physics and Mathematics, University of Eastern Finland, Box 111, FI-80101 Joensuu,
Finland
3 Department of Microelectronics and Applied Physics, Royal Institute of Technology (KTH), Elec-
trum 229, SE-164 40 Kista, Sweden
Near-Field Imaging of Interacting Nano-Particles
P35 Christian Jüngst*and Andreas Zumbusch 137
Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany
Label-free imaging of Lipid Droplets in living cells
P36 Marcelle Koenig1, Steffen Ruettinger1, Sebastian Tannert1, Thomas Schoenau1, 141
Christian Litwinski1, Olaf Schulz2, Michael Wahl1, Marcus Sackrow1, Kristian
Lauritsen1, Felix Koberling1, Robert Ros2, and Rainer Erdmann1
1 PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, info@picoquant.com
2 Arizona State University, Lab Robert Ros, Physics Department, Tempe, AZ 85287-1504, USA
Pushing the boundaries of single molecule detection
P37 Zeno Lavagninoa1, Francesca Cella Zanacchi1, Alberto Diaspro1,2 142
1 Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30,16163 Genova, Italia
2 LAMBS- Dept. of Physics – University of Genoa, Via Dodecaneso 33, 16153 Genova, Italia
Thick Sample imaging and scattering effects: a phantom sample based characteriza-
tion of Single Plane Illumination Microscopy performances
P38 Aimar Namma2, Andres Arend1, Marina Aunapuua,2 146
1 Department of Anatomy, University of Tartu, Ravila 19, 50411 Tartu, Estonia
2 Department of Veterinary Medicine, Estonian University of Life Sciences, Fr Kreutzwaldi 62, 51014
Tartu
Computer assisted image analysis in the detection of expression of bone morphoge-
netic proteins in the neural tube of human embryos
P39 Marco Zanella1†, Luca Ceseracciu2†, Roberta Ruffilli1, Alberto Diaspro2, Liber- 158
ato Manna1
1 Nanochemistry, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy
2 Nanophysics, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genova, Italy
Polarized plasmonic absorption in micrometer long copper(I) selenide nanowires
OWLS • Genoa, 4-6 July 2012 • Program • Page 21P46 - P60 Page
P46 Abebe Belay 122
Adama Science and Technology University, School of Natural Sciences, Department of Physics,
P.O.Box, 1888, Adama, Ethiopia,
The Hetero-Association of Caffeine (CA) with 5-Caffeoyl quinic Acid (5-CQA) and
Ethidium Bromide (EB) in Aqueous Solution
P47 Mariagrazia Di Luca1*, Francesco Cardarelli2, Ranieri Bizzarri 1,3 127
1 NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12 - 56127
Pisa
2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa
3 Istituto di Biofisica - CNR, via Moruzzi 1, 56124 Pisa
Fluorescent recombinant virus like particles as nano-carriers of sensors
P48 Sabato Fusco, Valeria Panzetta, Paolo Antonio Netti 132
Center for Advanced Biomaterials for Health Care@CRIB, Istituto Italiano di Tecnologia Largo Bar-
santi e Matteucci, 53 80125 Napoli
Cell Mechanical Characterization by Particle Tracking Microrheology in Optical Mi-
croscopy
P49 Gallus Lorenzo1*, Ferrando Sara1, Gambardella Chiara1, Amaroli Andrea1, 134
Faimali Marco2, Veronica Piazza2, Bianchini Paolo3, Masini Maria Angela1
1 Dip. di Scienze della Terra, dell’Ambiente e della Vita (DiSTAV), Univ. di Genova
2 Ist. di Scienze Marine, ICMM, CNR, Area di Genova
3 Dipartimento di Nanofisica, Istituto Italiano di Tecnologia Genova
Aquaporins in cyprid of barnacle Balanus amphitrite (=Amphibalanus amphitrite)
(Cirripedia, Crustacea)
P50 Gözde Aydoağan KILIÇ, Volkan KILIÇ, Ahmet ÖZATA 139
Anadolu University, Faculty of Science, Department of Biology, 26470, Eskişehir, TURKEY
Autometallographic localization of heavy metals and immunoperoxidase detection
of metallothionein induction in Common carp (Cyprinus carpio L.1758) from Sakarya
River (TURKEY)
P51 Keurison F. Magalhães1,3, Anderson R.L. Caires1*, Margarete S. Silva2, Samuel L. 145
Oliveira3
1 Grupo de Óptica Aplicada, Universidade Federal da Grande Dourados, Dourados, MS, Brazil
2 Grupo de Estudo e Desenvolvimento de Materiais, Universidade Estadual de Mato Grosso do Sul,
Dourados, MS,Brazil
3 Grupo de Óptica e Fotônica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS,
Brazil
The Role of Fatty Acid Methyl Esters on Optical Properties of Biodiesel
P52 Olena Obukhova1*, Anatoliy Tatarets1, Olga Kolosova1, Iryna Fedyunyayeva1, 147
Leonid Patsenker1,2, Ewald Terpetschnig2
1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001
Kharkiv (Ukraine)
2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA
Highly Photostable Biomedical Markers Based on Thiosquarylium Dyes
Page 22 • Program • OWLS • Genoa, 4-6 July 201 2P53 Silvio Panettieri1 Giovanni Signore2 Ranieri Bizzarri2 148
1 NEST, Istituto Nanoscienze - CNR, Piazza S. Silvestro 12, 56127 Pisa
2 Center for Nanotechnology Innovation @NEST, Istituto Italiano di Tecnologia, Piazza San Silvestro
12, 56127 Pisa
Synthesis, stereochemical characterization, and photophysical features of an innova-
tive 3-styryl coumarin acting as polarity/viscosity sensor for bioanalytical use
P54 Leonid Patsenker1,2*, Ewald Terpetschnig2, Anatoliy Tatarets1, Olena Obuk- 149
hova1, Olga Kolosova1, Larysa Markova1, Inna Yermolenko1
1 State Scientific Institution “Institute for Single Crystals”, NAS of Ukraine, 60 Lenin Ave., 61001
Kharkiv (Ukraine).
2 SETA BioMedicals, LLC, 2014 Silver Ct East, Urbana, 61801, IL, USA,
Next Generation Fluorescent Dyes for Biomedical Applications
P55 Alessia Petrelli, Claudio Canale, Alberto Diaspro and Silvia Dante 150
Nanophysics Department, Fondazione Istituto Italiano di Tecnologia, Genova (Italy)
Quantitative evaluation of cell adhesion on patterned surfaces
P56 Ferruccio Pisanello1,2*, Luigi Martiradonna1, Godefroy Leménager3, Alberto 151
Bramati3, Massimo De Vittorio1,4
1 Center for Bio-Molecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, 73010 Arne-
sano (Lecce), Italy
2 Center for Neuroscience and Cognitive Systems @UNITN, Istituto Italiano di Tecnologia, 38068
Rovereto (TN), Italy
3 Laboratoire Kastler Brossel, Université Pierre et Marie Curie, CNRS UMR 8552, Ecole Normale
Supérieure, 75005 Paris, France
4 National Nanotechnology Laboratory, CNR-‐Nano, Università del Salento, Dipartimento Ingegn-
eria dell’innovazione, 73100 Lecce, Italy
Anisotropic colloidal nanocrystals as ultrastable polarized quantum emitters
P57 Kelly-Ann Vere, Joanna Richens and Paul O’Shea 157
Cell Biophysics Group, Institute of Biophysics, Imaging and Optical Science (IBIOS), School of Biol-
ogy, University of Nottingham, UK NG7 2RD
Electrostatic mapping of molecular interactions on the cell surface; identification of
novel cell-surface receptor systems
OWLS • Genoa, 4-6 July 2012 • Program • Page 23OWLS Member Assembly
July 5th, 2012 in Genoa, Italy (17:30-19:00)
Porto Antico Congress Center – Magazzini del Cotone – Scirocco-Libeccio Room
Agenda
Extra content: Lecture by Francesco Pavone (see program)
1. Discussion of the Agenda
2. President’s Report
3. Update of bylaws
4. Elections
❚❚Board
❙❙ President, Vice-President, Secretary, Treasurer
❙❙ OWLS Representative to ICO
❚❚Regional Council Representatives
❙❙ Africa, North America, South America, Asia, Australia/New Zealand, Europe
❚❚Standing Committees
❙❙ Bylaws and Regulatory Statutes;
❙❙ Technology Transfer, Standardization, and Technology Assessment;
❙❙ Finances;
❙❙ Education and Ethics
5. Meeting schedule
❚❚OWLS 13 Location
❚❚Co-Sponsoring
6. Treasurer’s report and decision of formal approval
❚❚Financial Report
7. New Members
8. Publications
9. Dues
10. Committee Reports
11. Miscellaneous
Prof. Alberto Diaspro
President of the International Society on Optics Within Life Sciences (OWLS)
Page 24 • Program • OWLS • Genoa, 4-6 July 201 2You can also read
