Course description BIOLOGICAL ANALYSIS AND LABORATORY DIAGNOSIS - FACULTY OF PHARMACY
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Course description BIOLOGICAL ANALYSIS AND LABORATORY DIAGNOSIS 3RD YEAR, 2ND SEMESTER DEGREE: PHARMACY MODALIDAD: ON CAMPUS ACADEMIC YEAR: 2019/2020 FACULTY OF PHARMACY
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1. COURSE/SUBJECT IDENTIFICATION
1.- COURSE/SUBJECT:
Name: BIOLOGICAL ANALYSIS AND LABORATORY DIAGNOSTICS
Code: a311
Semester (s) when the course is taught: 1st
Year (s) course is taught: 3rd
Semester
Type: Compulsory subject ECTS: 6 Hours/ ECTS: 30
Language: English Modality: on campus
Degree (s) in which the course is taught: Pharmacy
School which the course is taught: Pharmacy
2.- ORGANIZATION OF THE COURSE:
Department: Química y Bioquímica (Coordinación de la asignatura) y Ciencias Farmacéuticas y de la
Salud.
Area of knowledge: Microbiology, Biochemistry and Molecular Biology. Parasitology and
Immunology.
2. LECTURERS OF THE COURSE/SUBJECT
1.- IDENTIFICACIÓN DEL PROFESORADO:
Responsible of the Course CONTACT
Name: Dra. Maria José Pozuelo
Phone (ext): 4714
Email: marpoz@ceu.es
Office: 216 edificio C
Profesor CONTACT
Name: Dr. Estanislao Nistal Villán
Phone (ext): 4714
Email: estanislao.nistalvillan@ceu.es
Office: 216 edificio C
Profesor CONTACT
Name: Dra. Carmen de Mendoza Fernández
Phone (ext):
Email: carmen.fernandez5@ceu.es
Office:
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Profesor CONTACT
Name: Dña Andrea Valderrey Barreal
Phone (ext): 4843
Email: andreadiana.valderreybarreal@ceu.es
Office: Tercera planta edifico C Farmacia
Profesor CONTACT
Name: Dra. Carmen del Águila de la Puente
Phone (ext): 913724770 (4770)
Email: cagupue@ceu.es
Office: A101
Profesor CONTACT
Name: Dra. Soledad Fenoy Rodríguez
Phone (ext): 913724721 (4721)
Email: sfenrod@ceu.es
Office: C219
Profesor CONTACT
Name: Dra. Ángela Magnet Dávila
Phone (ext): 913724721 (4721)
Email: angela.magnetdavila@ceu.es
Office: C219
Profesor CONTACT
Name: Dra. Dolores Ollero Baceiredo
Phone (ext): 913724779 (4779)
Email: mariadolores.ollerobaceiredo@ceu.es
Office: C108
Profesor CONTACT
Name: Dra. María Gracia Sánchez Alonso
Phone (ext): 913724792 (4792)
Email: msancheza@ceu.es
Office: C208
Profesor CONTACT
Name: Dr. Julio Sevillano Fernández
Phone (ext): 913726411 (4824)
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Email: jsevilla@ceu.es
Office: Relaciones internacionales planta 0 edificio C
2.- TUTORIAL ACTIONS:
For any queries students can contact lecturers by e-mail, phone or visiting their office during the
teacher’s tutorial times published on the students’ Virtual Campus.
3. COURSE DESCRIPTION
Theoretical-practical subject that aims to teach students the diagnostic criteria and the methodology
used in the laboratory for the diagnosis of infections caused by bacteria, viruses, fungi and parasites,
as well as hematological diseases. This subject is the basis of one of the specialties via FIR.
It consists of 3 modules: microbiology, parasitology and hematology. The evaluation of each module
will be done separately, being necessary to pass each of the modules to pass the subject.
It is strongly recommended that the students who enroll in this subject have taken the subjects of
Anatomy and Histology (1st year), Microbiology (3rd year) and Parasitology (3rd year).
4. COMPETENCIES
1.- COMPETENCIES:
Code Basic and General Competencies
CB1 Students show to possess and understand knowledge in an area of study that is based on
general secondary education at a level that, although it is supported by advanced
textbooks, includes some issues of the forefront of knowledge of that field of study.
CB2 Students apply their knowledge to their work or vocation in a professional manner, and
have competencies that can be demonstrated through devising and defending arguments
or solving problems within their field of study.
CB3 That students have the ability to gather and interpret relevant data (usually within their area
of study) to make judgments that include a reflection on relevant social, scientific or ethical
issues.
CB4 That students can transmit information, ideas, problems and solutions to a specialized and
non-specialized public.
Code General Competencies
CG10 That students have the ability to gather and interpret relevant data (usually within their area
of study) to make judgments that include a reflection on relevant social, scientific or ethical
issues.
CG12 Design, apply and evaluate reagents, methods and clinical analytical techniques, knowing
the basic fundamentals of clinical analysis and the characteristics and contents of
laboratory diagnosis reports.
CG13 Develop hygienic-sanitary analyzes, especially those related to food and the environment.
Code Specific Competencies
CE36 To know and understand the basic fundamentals of clinical analysis and the characteristics
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and contents of the laboratory diagnosis reports.
CE37 To develop hygienic-sanitary analysis (biochemical, microbiological and parasitological
bromatological), related to health in general and with food and the environment in
particular.
CE49 To know the analytical techniques related to laboratory diagnosis, toxic, food and
environment.
2.- LEARNING OUTCOMES:
Code Learning outcomes
RA102 Description of the main techniques useful for the diagnosis and monitoring of infectious and
hematological diseases.
RA103 Demonstration of the management of the methods of analysis in the diagnosis of the main
bacterial, parasitic and blood disorders infections.
RA104 Evaluation of the results obtained after cultivation / analysis of the biological samples,
distinguishing commensal microorganisms from pathogenic microorganisms.
RA105 Assessment of health and disease states after analysis of blood samples.
RA107 Apply biochemical, microbiological, parasitological and hematological methods in the
evaluation of useful magnitudes for the diagnosis of the main human pathologies
(metrology)
RA109 Interpret the clinical significance of the values obtained (semiology).
5. LEARNING ACTIVITIES
1.- DISTRIBUTION OF STUDENTS` ASSIGNMENT:
Total hours of the course 180
Code Name On-campus
hours
AF2 Seminars 1
AF4 Practice. Laboratory practice. 90
AF9 Tutorials 2
AF6 Evaluation Activities 2
Total Presence hours 95
Code Name Not on-
campus hours
AF0 Student independent work 85
2.- DESCRIPTION OF LEARNING ACTIVITIES:
Activity Definition
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AF2 Seminars Learning activity that is developed in the classroom-seminar, that under
the guidance of the professor, encourages the cooperative learning among
students and that is directed to the evaluation of cases and allows the
study in detail.
AF4 Laboratory Learning activity that is developed in suitable facilities outside the
Práctices classroom, under the guidance of the class professor, encourages
independent and / or cooperative learning of the student and, by
appropriate design is aimed at the execution of a theoretical research or
technical, practical or artistic work.
AF9 Tutorials Training activity developed by the teacher attending a student to answer
questions or guide the learning process. The objectives of teacher tutoring
are: solving doubts, proposing practical cases, analyzing results obtained,
reviewing exercises, projects, work, practices, teaching guidance on
problems raised and how many others contribute to the student's progress in
their learning process.
AF6 Evaluation Activities that the student must develop in person, such as attendance to
activities lectures and seminars integrating the subject.
6. ASSESMENT OF LEARNING
1.- CLASS ATTENDANCE:
• 100% attendance at practical classes is mandatory. In the case that for justified reasons
the student cannot attend classes, he / she must recover the practice in another group with
the approval of the teacher. The student will be examined when he has completed 100% of
the subject..
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2.- SYSTEMS AND EVALUATION CRITERIA IN ORDINARY AND EXTRAORDINARY CALL
ORDINARY CALL (Continuos evaluation) 1
Code Name Minimum score 2 Percentage
SE1 Questions type exam and / or test. 5 in each module 25,5%
of the subject
SE1 Questions type exam and / or test. (ordinary call) 4 10%
SE3 On-site evaluation of various training activities 5 in each module 21%
(practical exam). of the subject
SE3 On-site evaluation of diverse training activities 12%
(performance in the laboratory).
SE5 Practical or clinical cases. 5 in each module 22,5%
of the subject
SE7 Practice notebook 9%
(2) Minimum score to be able to apply to the indicated percentages.
3.- DESCRIPTION OF ASSESSMENT CRITERIA:
Assessment criteria Definition
Questions exam and / or test There will be a written theoretical exam at the end of
each module of the subject (microbiology, parasitology
and hematology), and at the end of the course on the
date of the ordinary call. This exam will be inclusive of
the subject, so it will include questions from the three
modules studied. In the exams of each module it will be
necessary to have a grade of 5 to pass the module and
in the integrative exam a minimum grade of 4 will be
necessary for the percentages to be applied.
On-site evaluation of several training It will be carried out at the end of each module of the
activities subject (microbiology, parasitology and hematology) to
know if the student has acquired the practical skills
necessary to carry out the laboratory diagnosis.
It will be necessary to have a grade of 5 in the exam of
each module to pass the module and the subject.
Likewise, the student's performance will be evaluated
independently during the internship days in each
laboratory.
Practical or clinical cases. The resolutive capacity of clinical cases related to the
subject taught will be assessed through a written
examination.
It will be necessary to have a grade of 5 in the exam of
each module to pass the module and the subject.
Practice notebook It will include the laboratory work of the student during
the practices. This notebook will not be corrected
independently of the grade obtained by the student in
the exam.
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7. COURSE PROGRAMME
1.- COURSE PROGRAMME:
THEORETICAL-PRACTICAL PROGRAM:
-Introduction to the subject: A general seminar will be held on the objectives of the subject and its
contents. The competences that are intended to be developed with the subject will be explained and the
doubts that arise among the students will be solved.
MICROBIOLOGY
1.- Urine microbiological analysis:
- Quantitative urine cultures:
- Urine culture evaluation. Isolation and identification of pathogens of the urinary tract using
automatic methods. Study of the antibiotic sensitivity of pathogenic microorganisms by disc diffusion
techniques and automatic techniques.
- Evaluation of urinary infection by Gram stain of the urine.
2.- Microbiological analysis of respiratory samples:
- Study of pharyngeal exudate: Study of the pharyngeal microbiota (Streptoccoccus viridans,
Staphylococcus aureus) and pathogenic (Streptococcus pyogenes and Streptococcus B hemolytic
groups C and G of R.Lancefield). Detection and identification by various techniques: agglutination
with antibodies, antibiotic sensitivity, metabolic tests, etc. Study of the antibiotic sensitivity of
pathogenic microorganisms.
-Sputum study: microscopic evaluation of different samples with pathogenic microorganisms:
Streptococcus pneumoniae, Haemophilus influenzae, and Mycobacterium tuberculosis.
3.- Microbiological analysis of samples of the female genital tract (vaginal exudate):
- Isolation and identification of Candida albicans and Streptococcus agalactiae. Antibiotic sensitivity
study.
- Microscopic study of normal and pathological vaginal samples.
4.- Microbiological stool analysis:
- Coproculture. Study of the microbiota and identification of possible pathogens such as Salmonella
and Shigella. Study of the antibiotic sensitivity of pathogenic microorganisms.
5- Microbiological analysis of cerebrospinal fluid (CSF):
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- Microscopic study of normal and pathological CSF. Visualization of preparations with pure cultures
of the main etiological agents of meningitis: Neisseria meningitidis, Haemophilus influenzae and
Streptococcus pneumoniae.
6- Study of viral infections using immunochemical and molecular techniques.
Parasitology
1.- Macroscopic Analysis. Isolation, mounting, and conservation of parasites. Mounting of
Nematodes, Cestodes, and Arthropods. Observation of permanent preparations.
2.- Coprological Diagnosis I. Direct observation.
3.- Coprological diagnosis II. Concentration methods.
4- Coprological diagnosis III. Realization of permanent preparations. Kinyoun and Trichromic stains.
5.- Special techniques in coprology. Graham test (tape test). Agar plate method, Stoll, Kato-Katz for
the diagnosis of hookworm and Strongyloides
6.- Techniques for the detection of blood parasites. Direct examination, staining: thin extensions and
thick drop. Giemsa and Wright. Specific identification of Plasmodium species. Morphological
differentiation by staining. QBC-hematocrit, immunochromatography and other methods.
7.- Techniques for the diagnosis of genito-urinary parasitism. Thawing and culture of vaginal
trichomonads, counting, and staining.
8.- Techniques for the diagnosis of tissue parasitism. Digestion of Trichinella spiralis.
9.- Methods of immunological diagnosis and molecular biology in clinical parasitology applied to
parasites I. Diagnosis of Leishmania spp.
10.- Immunological diagnostic methods applied to parasites II. Diagnosis of Toxoplasma gondii.
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HEMATOLOGY
1. Peripheral blood test. Red blood cell count and reticulocytes. White blood cell count.
Preparation and staining of extensions.
2. Hematimetric analysis: determination of hemoglobin and hematocrit. Corpuscular indices:
alterations in the size and chromia of erythrocytes
3. Diagnosis of alterations of the red series. Erythrocytosis and erythrocytopenia. Regenerative
and non-regenerative anemia.
4. Diagnosis of alterations of the white series. Differential count of white blood cells.
Leukocytosis and leukopenia.
5. Oncohematology: diagnosis of acute leukemia; myeloproliferative syndromes;
lymphoproliferative syndromes; myelodysplastic syndromes.
6. Diagnosis of alterations of the thrombocytic series. Thrombocytosis and thrombocytopenia.
7. Laboratory tests for the analysis of hemostasis. Prothrombin time. Activated partial
thromboplastin time.
8. BIBLIOGRAPHY OF THE SUBJECT
1.- BASIC BIBLIOGRAPHY:
MICROBIOLOGy
• MURRAY PR, ROSENTHAL KS, PFALLER MA. (2016). Medical Microbiology. 8ª Edición.
Editorial Elsevier Saunders.
PARASITOLOGy
• GARCÍA, L. S. (2015). Diagnostic Medical Parasitology. 6ª Edición. ASM Press.
HEMATOLOGy
RODAK, FRITSMA, KEOHANE (2014). Hematología Fundamentos y Aplicaciones Clínicas.
4ª Edición. Editorial Médica Panamericana.
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2.- ADDITIONAL BIBLIOGRAPHY:
MICROBIOLOGY
• PRATS G. 2005. Microbiología Clínica. Ed Panamericana
• PRATS, G. 2012. Microbiología y Parasitología Médicas. Ed. Panamericana.
• TORTORA, G.J., FUNKE, B. R., C. L. 2010. Microbiology. Ed. Médica Panamericana.
PARASITOLOGY
• ASH, L.A.; ORIHEL, T.C.: 1987 Parasites. A guide to laboratory procedures and
identification. ASCP Press.
• BAILENGER, J. 1982. Coprologie Parasitaire et founctionelle.4ª Ed. Bordeaux.
• FLECK, S.L.; MOODY, A.H. 1988. Diagnostic techniques in medical parasitology. Wright
• GARCIA, L. 1999.Practical guide to diagnostic parasitology. ASM Press..
• GUILLESPIE, S.H.; HAWHEY, P.M. 1995.Medical Parasitology: A Practical Approach. RL
Press.
• HALTON, D.W.; et al. 2001.Practical Exercises in Parasitology. Cambridge.
• OMS. 1992. Métodos Básicos de Parasitología Médica.
• PETERS, W., PASVOL, G.2008. Atlas de Medicina Tropical y Parasitología. Elsevier-Mosby
• PRICE, D.L. 1994. Procedure manual for the diagnosis of intestinal parasite. CRC Press.
• parasitology.dmu.ac.uk
HEMATOLOGY
• SAN MIGUEL, J SÁNCHEZ-GUIJO, F. (2015). Hematología. Manual básico razonado.
Cuarta edición. Elsevier. ISBN: 9788490228265
• WILLIAMS, W.J. (2014): Manual Williams de Hematología. 8ª edición. Mc Graw Hill
Interamericana. Mexico. ISBN : 9786071510006
• VIVES I CORRONS, J.L. (1999): Manual de técnicas de laboratorio en Hematología. Salvat.
Barcelona.
4.- RECURSOS WEB DE UTILIDAD:
• Microbiology and Parasitology:
http://www.seimc.org/documentoscientificos.php?mn_MP=3
http://www.bacteriainphotos.com/bacteria%20photo%20gallery.html
http://www.who.int/zoonoses/diseases/en/
http://www.cdc.gov
http://www.semtsi.es
• Hematología: http://hematologyoutlines.com/atlas.html
http://imagebank.hematology.org/
9. ATTITUDE IN THE CLASSROOM
1.- RULES:
Any irregular act of academic integrity (no reference to cited sources, plagiarism of work or
inappropriate use of prohibited information during examinations) or signing the attendance
sheet for fellow students not present in class will result in the student not being eligible for
continuous assessment and possibly being penalized according to the University regulations.
The students that use any non-authorized information during the exam will lose the possibility
to pass the examination they are taking (ordinary and extraordinary).
Cell phones will only be allowed when the professor gives explicit permission.
It is not allowed to bring coats, bags and folders into the laboratory. The student will have
available lockers in order to keep the material. The student will need to bring a lock.
Los alumnos que utilicen alguna información no autorizada durante el examen (chuletas en
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papel, en bolígrafos, en la piel, en el móvil, etc.) irán a convocatoria extraordinaria con toda la
asignatura, independientemente de los bloques que hayan aprobado en convocatoria
ordinaria.
El uso de los teléfonos móviles se realizará únicamente cuando el profesor lo permita.
Está prohibida la entrada de abrigos, bolsos, carpetas al laboratorio, debiéndolos depositar
previamente en las taquillas. El alumno deberá traer su propio candado.
It is imperative that the student wear lab coat and hair collected to work in the laboratory.
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