NUTRA BLEND NEWS 2020 - summer/fall

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NUTRA BLEND NEWS 2020 - summer/fall
NUTRA BLEND NEWS
2020 - summer/fall
NUTRA BLEND NEWS 2020 - summer/fall
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               PREBIOTICS IN LIVESTOCK PRODUCTION SYSTEMS: A FUNCTIONAL ROLE FOR                                        AL PEDERSON
               NATURAL CARBOHYDRATES                                                                                         MN, ND
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2
NUTRA BLEND NEWS 2020 - summer/fall
PREBIOTICS IN LIVESTOCK PRODUCTION SYSTEMS:
A FUNCTIONAL ROLE FOR NATURAL CARBOHYDRATES
writer // Richard Murphy, Ph.D./ Research Director / Alltech Bioscience Centre, Dunboyne, Co. Meath

Targeted nutrition, starting with the                                structure, enabling the feed additive to act as                      of Bio-Mos® 2 enables the gut to “police”
development and maintenance of the                                   a decoy, providing a first level of protection                       itself through promotion of microbial
intestine, results in the production of stronger,                    by blocking attachments to intestinal cells                          diversity, thereby reducing issues associated
healthier animals. For many years now, it has                        (Jacques and Newman, 1994).                                          with pathogen colonization.
been known that the use of carbohydrates
isolated from the cell wall of specific yeast                        Distinct from original first-generation MOS                          A drawback of antibiotics is their non-specific
strains plays a central role in modulating                           products such as Bio-Mos, the second-                                effect on the microbiome and the reduction
immune responses, preserving gut tissue                              generation Bio-Mos® 2 is best described                              in overall microbial diversity noted with
integrity and eliminating pathogens from the                         as a natural growth permitter. Bio-Mos® 2,                           their use. Given the ability of Bio-Mos® 2 to
intestine.                                                           a bioactive mannose-rich fraction (MRF), is                          enhance the overall gut microfloral diversity,
                                                                     derived from the outer cell wall of a specific                       it is an ideal solution to combat the negative
The intestinal microbiome plays several                              strain of yeast selected by Alltech (Spring et                       consequences         of   diversity  reduction
crucial roles in the animal, including the                           al, 2015). Depending on the target species,                          following antibiotic administration.
regulation of cell turnover in the gut wall,                         their ages and the protocol chosen, Bio-
competition for nutrients, the modification                          Mos® 2 is included in ruminant diets at a                            When comparing first- and second-
of digestion, competitive exclusion of                               rate from 2 to 20 grams per head per day.                            generation MOS- and MRF-based products
pathogens, the metabolism of mucus                                   Due to its enhanced bioactivity, it can be                           such as Bio-Mos® and Bio-Mos® 2, it is
secretions and modulation of mucosal                                 supplemented at lower levels than those                              important that the end user understands
immunity. It is critical that the microbiome                         typically seen when using generic MOS                                that form defines function. Individual MOS-
functions to its fullest capability in order to                      products.                                                            and MRF-based products will have widely
maintain gut health and maximize growth.                                                                                                  differing compositions, and while attempts
                                                                     The effects of Bio-Mos® 2 supplementation                            have been made to compare products based
Mannoproteins and mannanoligosaccharides                             on health and performance have been                                  on their total mannose content, studies have
account for more than 40% of the                                     studied comprehensively, and this second-                            shown that not only the extent but the type
carbohydrate in the cell wall of Saccharomyces                       generation MRF product has proven                                    of carbohydrate branching in these products
cerevisiae and are one of the most widely                            extremely effective at improving weight gain                         is the key factor in controlling efficacy.
studied functional prebiotics used in                                and feed conversion efficiencies while also                          Essentially, not all MOS or MRF products will
animal feeds. The application of prebiotic                           supporting gut integrity and strengthening                           act in the same manner. Innovative analytical
mannan structures present in yeast cells                             defenses against pathogens.                                          tools developed by Alltech, such as ELMAA
fed to animals was first studied by Alltech                                                                                               and ELLA, enable the detection of Bio-Mos®
in 1993 with the development of Bio-Mos®                             More recent studies on Bio-Mos® 2 have                               2 in feed and measure its biological activity,
(Newman et al 1993). An extremely large set                          focused on the product’s effects on the                              allowing for traceability and quality control
of studies clearly establishes that the use of                       overall bacterial community of the gut — not                         management.
first-generation mannose-based (MOS) cell                            just specific bacteria — and such work has
wall fractions, such as Bio-Mos®, in animal                          shown that supplementation with Bio-Mos®                             Having spent more than 30 years researching
feeding strategies can have beneficial effects                       2 can modulate intestinal microflora (also                           gut health, Alltech is recognized for its
on animal health and productivity (Spring et                         known as the microbiome), enabling it to                             leadership and expertise in the field of
al, 2015).                                                           function to its fullest capability (Corrigan and                     glycomics, with the accumulation of more
                                                                     Murphy, 2016; Corrigan et al, 2018, Franklin                         than 700 research studies, including over
The best-characterized activity of Bio-Mos®                          et al, 2005; Heinrichs et al, 2003; Quezada                          150 peer-reviewed papers, on the effects of
is its ability to agglutinate or aggregate                           et al, 2007).                                                        carbohydrate fractions such as Bio-Mos® 2
pathogenic bacterial cells containing Type 1                                                                                              in animal production systems (Spring et al,
fimbriae. In the gastrointestinal tract, lectins                     Commercial trial work using Bio-Mos® 2 has                           2015).
(carbohydrate-binding proteins) on bacterial                         demonstrated that gut function becomes
cells bind to complementary carbohydrates                            normalized following the enhancement of                              Closing the gap between ideal and actual
on animal epithelial cell surfaces. This                             overall microbial diversity. Reproducible and                        performance is essential to maximize
process is critical to the initiation of                             consistent alterations in microbiota have                            profitability. Bio-Mos® 2 is a cost-effective,
bacterial colonization and any associated                            been noted, resulting in enhanced microbial                          safe, traceable and natural growth permitter.
pathogenesis. Bacterial pathogens can                                diversity. The greater the diversity of the                          From start to finish, it is a key part of a
also attach to Bio-Mos® due to its unique                            microbes, the healthier the gut, and the use                         strategic feeding program.

References
Corrigan, A., Russell, N., Welge, M., Auvil, L., Bushell, C., White, B. A. and Murphy, R. A. (2018) The use of random forests modelling to detect yeast-mannan sensitive bacterial changes in the broiler
cecum. Scientific Reports Vol. 8, Article number: 13270 https://rdcu.be/5Q6g
Corrigan, A., and Murphy., R. (2016). Effect of dietary mannan-rich fractions on bacterial communities, a new approach with old data. 67th Annual Meeting of the European Federation of Animal Science,
Belfast, Ireland.
Franklin S., Newman M., Newman K. and Meek K. (2005). Immune parameters of dry cows fed mannan oligosaccharide and subsequent transfer of immunity to calves. Journal of Dairy Science, 88:
766–775.
Heinrichs A.J., Jones C.M. and Heinrichs B.S. (2003) Effects of mannan oligosaccharide or antibiotics in neonatal diets on health and growth of dairy calves. Journal of Dairy Science, 86: 4054–4069.
Jacques K.A. and Newman K.E. (1994) Effect of oligosaccharide supplements on performance and health of Holstein calves pre- and postweaning. Journal of Animal Science, 72 (Suppl. 1): 295.
Newman K.E, Jacques K.A. and Buede R. (1993) Effect of mannanoligosaccharide on performance of calves fed acidified and nonacidified milk replacers. Journal of Animal Science, 71(Suppl. 1): 271.
Quezada V.C., Babatunde B.B. and Frankel T.L. (2007) Effect of mannan-oligosaccharides on the mucosal immune system of dairy calves. Journal Animal Science, 85 (Suppl.1): 211.
Spring, P., Wenk, C., Connolly, A., & Kiers, A. (2015). A review of 733 published trials on Bio-Mos®, a mannan oligosaccharide, and Actigen®, a second-generation mannose rich fraction, on farm and
companion animals. Journal of Applied Animal Nutrition, 3, E8. doi:10.1017/jan.2015.6.

4
Backed by science for them.
Healthier calves for you.

Bio-Mos® 2 continues the legacy of Bio-Mos® by improving
upon Alltech’s trademark technology. Formulated to feed
the gastrointestinal tract, Bio-Mos® 2 increases support
for gut integrity and colostrum quality to better promote
overall performance at all stages of cattle production.

    Learn more at Alltech.com/Bio-Mos2

Alltech.com           AlltechNaturally           @Alltech
© 2020. Alltech, Inc. All Rights Reserved. US_DA_BioM2_ad1
Normal = healthy, and
it starts with what goes in.

                BOVAMINE® brand probiotic products
                support normal GI functions in cattle.
                They can be a natural, safe, sustainable
                part of your solution.

                Visit www.chr-hansen.com to find the
                product best suited to your needs.

            BOVAMINE® Defend is listed in the BIFSCO pre-harvest library as a product which has
            met the established requirements for listing.
THE ROLE OF CATTLE MANAGEMENT IN THE BATTLE
THE BEEF INDUSTRY FACES AGAINST SALMONELLA
writers // Dr. Jennifer Schutz, Allen McDonald, and Jim Turner

Salmonella is a challenging pathogen                                they are challenged with stress factors                            has been shown to significantly
to control as it can affect cattle within                           such as heat, diet changes, or other                               reduce the shedding of Salmonella.
every stage of production. From                                     immune challenges. Older calves                                    Additionally, a research trial conducted
pregnant cows to feedlot cattle,                                    will also have high temperatures and                               at Texas Tech University found that
Salmonella can cause economic                                       severe diarrhea which can contain                                  LA51 reduced the prevalence and
issues for producers. In addition to                                blood, mucus, and shreds of intestinal                             concentration of Salmonella in lymph
the burden of Salmonella to the live                                lining. Severe dehydration and                                     nodes. The benefit of using LA51 as a
animal, it also has major economic                                  depressed feed intakes will also occur.                            pre-harvest intervention may also have
issues on the beef production side                                  Illness will usually last 7-10 days with                           benefits to the producer as increased
as Salmonella is a leading cause of                                 recovery taking between two to three                               performance, reduced late term
foodborne illness. Research has found                               weeks. One of the major issues with                                dead’s, and overall health benefits
that Salmonella has a unique ability                                calves infected with Salmonella is that                            to the cattle are often observed
to survive within the gastrointestinal                              some will become carriers and may                                  and documented within numerous
tract and lymph nodes of cattle for                                 shed the pathogen for prolonged                                    research trials.1
long periods of time; because of                                    periods of time. Unfortunately,
this, determining how to manage                                     Salmonella is unique in the fact that                              Conclusion
Salmonella is absolutely critical to                                it can survive and replicate within the                            It is crucial that the beef industry
herd health and food safety concerns.                               very immune system responsible for                                 continue to find ways to reduce
                                                                    killing it. Salmonella contamination                               Salmonella prevalence within our beef
Detection of Salmonella                                             is challenging to manage as cattle                                 supply. The major challenge facing
Based upon NAHMS 2011 feedlot                                       shedding the pathogen pose risk to                                 this goal is keeping Salmonella from
data, a very low percentage of all                                  other high-risk calves.                                            entering the immune system. Proper
feedlots monitor for Salmonella                                                                                                        pen and nutritional management can
(6.9%). Researchers have found that                                 Approaches                for       reduction             of       help reduce some of these issues.
milk production and performance                                     Salmonella                                                         Keeping pathogens from breaking
can decrease due to Salmonella but                                  Treatment of Salmonella is very                                    through the gastrointestinal tract
rarely are there easy to detect signs of                            challenging and not very successful.                               and supporting the immune system
infection.                                                          Management plays a tremendous                                      of these cattle are critical barriers
                                                                    role in the effectiveness of reduction                             that also need to be managed. One
Although Salmonella is usually                                      of this pathogen. Because Salmonella                               option for producers looking for an
undetectable in feedlot cattle, younger                             is transferred from animal to animal                               economical way to reduce pathogen
calves are very susceptible to death                                via fecal-oral route, anything that can                            prevalence while also supporting the
from this disease. Colostrum-deprived                               potentially spread this bacteria must                              overall health of cattle is BOVAMINE
calves are at highest risk of developing                            be properly cleaned and managed. In                                DEFEND®. Salmonella reduction
severe symptoms such as high fevers                                 addition to key hygiene management                                 will ultimately take producers and
(105°-107°F), smelly, watery diarrhea,                              practices, research has found that                                 packers working together to develop
dehydration and death within one to                                 specific probiotics can also help                                  and implement multiple hurdled
two days. As calves get older, they are                             reduce the shedding of Salmonella.                                 approaches for the reduction of this
less at risk of death, but can still show                           Lactobacillus animalis (LA51), the key                             pathogen from both the pre- and
clinical signs of the pathogen when                                 strain within BOVAMINE® products,                                  post-harvest sides.

References
1
  Reduced Burden of Salmonella entericain Bovine Subiliac Lymph Nodes Associated with Administration of a Direct-fed Microbial Vipham et al. - Zoonoses and Public Health - 2015

                                                                                                                                                                                   7
MAKING HIGH-QUALITY TMR: TIPS TO PROTECT FINISHED FEED
writer // Eugene Rodberg / sr. product manager / Kemin Animal Nutrition & Health – North America

Total mixed rations (TMR) as           microorganisms is fueled by             Measuring       temperatures     of
we know them today are an              nutrients in the TMR that were          the TMR and taking readings
invention of primarily the last 50     targeted for the dairy cow.             throughout the day is a great
years. Prior to TMR, dairy herds                                               way to monitor secondary
were fed predominately forages         Additionally, a byproduct of            fermentation. Producers are often
with a limited amount of grain         microorganism      growth     and       surprised to learn how quickly the
delivered at milking time. Dairy       metabolism is heat. Thus, not           TMR heats up. In just a few hours,
producers started moving toward        only do the non-beneficial              wild yeast and mold counts grow
TMR feeding as milk production         microorganisms      reduce     the      exponentially. Feed samples,
per cow increased, herds became        nutrient density of the TMR,            sent to a lab for analysis, may not
larger, free-stall and large-group     they also render the TMR less           represent the extreme situation
handling of cows became more           desirable to the cow as the ration      of a cow’s experience. Your
common, and milking parlors            temperature increases.                  best indication of a problem is
became more prevalent. The                                                     to monitor the TMR temperature
earliest known abstract reports        What causes the ration to heat?         and watch how the cows eat their
in the Journal of Dairy Science        Well fermented silage resists           ration. When the cows back off,
on feeding “complete rations” or       heating when exposed to fresh           feed quality may be the issue.
TMR may have appeared in the           air (oxygen), a condition called
1950s. The earliest full-length        aerobic stability. Silages that         The quality of a TMR can
article on TMR in the journal was      take longer to heat after facing        degrade quickly in summer
published in 1966.                     are considered more aerobically         conditions.          Preventing
                                       stable than silages that heat           secondary fermentation and its
Challenges                             quickly. In addition to aerobic         accompanying heat spike is so
The challenge associated with          stability, blending different feed      important and is especially true
feeding cows these nutritionally       ingredients in a TMR allows             if the forage or feed quality is
complete diets is we are also          microbes access to additional           already marginal.
feeding a complete diet to             nutrients along with air. This
beneficial and non-beneficial          process, along with a hot feed          Key       feed      management
microorganisms. When non-              bunk and higher humidity, creates       practices to keep feed fresh:
beneficial wild yeast, mold and        the perfect growing conditions for      • Feed twice per day. If possible,
bacteria grow in the TMR, thermal      molds and wild yeast to rapidly           provide fresh feed when
and aromatic related off-feed          grow. The fermentation of the             conditions are relatively cool.
events, milk fat depression and        feed generates heat and in                Consider offering two-thirds of
other digestive upsets are likely      return, increases the temperature         the TMR in the late afternoon/
to occur.                              of the TMR. It’s also possible a          early evening and one-third in
                                       dairy is adding more moisture to          the morning.
Summer      temperatures     and       the ration by adding green chop         • Push-up feed frequently.
humidity increase the challenges       forages or seasonal wet by-             • Carefully manage the face of
posed by the growth of mold and        products which are not ensiled.           an upright silo, bag or bunker.
bacteria in the TMR. Summer            The moisture content of the TMR           Removing 12 inches a day is
heat and humidity dramatically         may also increase as water from           recommended, and it is best to
increase the growth of these           cow cooling sprinklers drifts onto        remove from the entire face.
non-beneficial microorganisms.         the feed.                               • Use preservative products such
Of course, the growth of these
8
as bacterial inoculants and a                                        molds or wild yeast. For example,     silage was stable for 35.3 hours
   blend of organic acids at the                                        propionic acid will control some      before heating. Silage treated
   time of harvest.                                                     molds species but is ineffective      with a blend of organic acids was
 • Always use a thermometer to                                          on other strains. At the same time,   stable for 49.3 hours. In addition,
   check temperatures. Feeling                                          propionic acid has limited efficacy   the application of organic acids
   the temperature of the feed                                          on wild yeast. A mold inhibitor       reduced the peak temperature of
   with your hand will not give you                                     with a higher total acid content      the corn silage when exposed to
   accurate results.                                                    containing acetic, benzoic and/       air.
 • Use      highly     concentrated,                                    or sorbic acids has demonstrated
   buffered, broad-spectrum mold                                        efficacy in controlling yeast         Summary
   inhibitors. Buffering the blended                                    growth. Multiple acid formulations    Weather is unpredictable. When
   organic acids makes them less                                        are highly effective at controlling   labor is limited, feed quality is
   corrosive and much safer to                                          yeast.                                not optimal, and when conditions
   handle.                                                                                                    dictate, incorporating a premium
                                                                        Research conducted at the             mold inhibitor into the TMR can
 Research and solutions                                                 University of Delaware evaluated      help to protect your investment.
 If wild yeast is a problem, adding                                     the use of a buffered blend of
 an organic acid blend with                                             organic acids (propionic acid,        For more research or product
 propionic acid and other acids                                         potassium sorbate, and sodium         information, please visit
 to mixed feed will be highly                                           benzoate; 87% active ingredients)     kemin.com/feedquality or email
 effective at controlling secondary                                     to reduce TMR heating and             keminag@kemin.com.
 fermentation and heating of the                                        improve aerobic stability of
 TMR. Single acid treatments often                                      corn silage. When silage was          References      available    upon
 fail to control a wide variety of                                      exposed to air, untreated corn        request

KEMIN IS THE
SCIENCE BEHIND
HEALTHIER
CATTLE
Since 1961, Kemin has utilized molecular science to develop
industry-leading solutions for gut health, ruminant nutrition
and feed quality. It’s just one of the ways we sustainably
transform the quality of life every day for 80% of the
world with our products and services.

                                               kemin.com/cattle
                                               #TheScienceBehind
                                                                                                                                               9
© Kemin Industries, Inc. and its group of companies 2020.
All rights reserved. ® ™ Trademarks of Kemin Industries, Inc., U.S.A.
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[ ADVERTISEMENT ]

                                           Knowledge Is Power
 Unraveling the complexities of liver abscess disease can help us optimize its management.

A     full understanding of the liver
       abscess disease complex is elusive, as
 is determining when and how to best
                                                                 at the feedyard that may affect outcomes
                                                                 and quantify trim losses and carcass
                                                                 characteristics associated with liver
                                                                                                                                                                                                have a higher prevalence of liver abscesses
                                                                                                                                                                                                compared to others, such as Holsteins.
                                                                                                                                                                                                One possible explanation is that Holsteins
 manage it. “Knowledge is key. If we                             abscesses. In total, 161,491 livers were                                                                                       spend more days on feed than beef cattle
 don’t know how and why abscesses are                            evaluated from seven fed-beef processors                                                                                       (roughly 350 days vs. approximately 200
 formed, we won’t know how to best control                       in the Central Plains, Desert Southwest,                                                                                       days). More research needs to be done to
 them,” says Scott Laudert, Ph.D., SBL Tech                      High Plains, Northeast, Northern Plains                                                                                        determine why these cattle appear to be
 Consulting. In addition to understanding                        and Pacific Northwest.                                                                                                         more susceptible.
 current management strategies, research
                                                                The average liver abscess incidence for                                                                                         “This disease is multifactorial,” Laudert
 efforts must continue to discover new
                                                                cattle harvested at fed-beef processing                                                                                          agrees. “In addition to feedlot region, cattle
 ways to make liver abscess management
                                                                plants was 21.3%. Incidence was lowest in                                                                                        type, time of year, the diet composition and
 more effective. Casey Maxwell, Ph.D.,
                                                                the Northeast (10.0%) and highest in the                                                                                         when and how it’s fed to the cattle can all
 operations analyst at Cactus Feeders,                          Pacific Northwest (33.8%) (Figure 1).                                                                                            combine to affect incidence. Because there
 adds, “Given the increasing pressure for
                                                                                                                                                                                                 are no outward signs of the disease, there’s
 accountability when using antibiotics                            Figure 1.
                                                                                                                                                                                                 no way to pinpoint which factor is most
 in cattle, understanding more about the                              Average Fed Cattle Liver Abscess Prevalence
                                                                                                                                                                                                 important. This isn’t a straightforward
 disease can help us optimize how and when                                         40
                                                                                                                                                                                                 disease, so there are no easy fixes.”
 to use them.”                                                                          33.8
                                                               Percent Abscesses

                                                                                   30
                                                                                                             25.5             24.3
                                                                                                                                                                                                “Given the increasing pressure for accountability
“Because there are no outward signs of the                                         20                                                            18.5
                                                                                                                                                                                                 when using antibiotics in cattle, understanding
                                                                                                                              Desert Southwest
                                                                                         Pacific Northwest

                                                                                                                                                                    15.5
                                                                                                                                                  Northern Plains

disease, there’s no way to pinpoint which factor
                                                                                                             Central Plains

                                                                                                                                                                                   10.0          more about the disease can help us optimize
                                                                                                                                                                     High Plains

                                                                                   10
is most important. This isn’t a straightforward
                                                                                                                                                                                    Northeast

                                                                                                                                                                                                 how and when to use them.”
disease, so there are no easy fixes.”                                               0                                                                                                           — Casey Maxwell, Ph.D., Cactus Feeders
— Scott Laudert, Ph.D., SBL Tech Consulting

                                                                When evaluated by cattle type, fed                                                                                              “Currently, Tylan® (tylosin) is the most
 A huge price to pay                                            Holsteins had a higher abscess incidence                                                                                         efficacious tool available for reducing
 Liver abscesses continue to be a costly                        rate (25.0%) than fed beef steers (18.2%) or                                                                                     the incidence of liver abscesses in cattle,”
 problem for the industry through the loss                      fed beef heifers (19.1%).                                                                                                        says Maxwell. Elanco remains committed
 of condemned liver and other viscera
                                                               Complex, variable and puzzling                                                                                                    to learning more about the liver abscess
 because of reduced animal performance,
                                                              “The liver abscess disease complex has                                                                                             disease complex, developing strategies
 lower carcass yields and decreased
                                                               so much variability,” says Maxwell. “For                                                                                          that help ensure judicious antibiotic use
 processor efficiency. Using conservative
                                                               example, we can see drastically different                                                                                         and searching for new solutions.
 estimates, viscera losses are approximately
                                                               incidence levels from one pen of cattle to
 $60 million per year.1                                                                                                                                                                         Visit elanco.us or contact your Elanco
                                                               another that have come from the same
                                                                                                                                                                                                sales representative to learn more
 Surveying for liver abscess incidence1                        location and that have been managed the
                                                                                                                                                                                                about Tylan.
 Liver abscess prevalence trials were                          same way.” Another unanswered question
 conducted to help determine conditions                        is why certain breeds and types of cattle

 The label contains complete use information, including cautions and warnings. Always read, understand and follow the label and use directions.
 Tylan® (tylosin)
 CAUTION: Federal law restricts medicated feed containing this veterinary feed directive (VFD) drug to use by or on the order of a licensed veterinarian.
 For reduction of incidence of liver abscesses associated with Fusobacterium necrophorum and Arcanobacterium pyogenes: Feed continuously
 as the sole ration at 8 to 10 g/ton of tylosin (90% DM basis) to provide 60 to 90 mg/hd/d.
 Elanco supports the use of shared-class antibiotics for therapeutic uses while under the oversight of a veterinarian.
 Visit https://www.elanco.com/healthy-purpose#responsible-use-of-antibiotics for more details on Elanco’s Antibiotic, Welfare and Sustainability Policies.
 1
     Ryan Travis Herrick dissertation, West Texas A&M University. 2018.
 Tylan, Elanco and the diagonal bar logo are trademarks of Elanco or its affiliates.
 © 2020 Elanco.
 PM-US-20-0339
[ ADVERTISEMENT ]

    Smart Strategy Makes a Difference
                   Strategic use of Tylan® (tylosin) can effectively control liver abscesses.

D    espite the ongoing commit-
     ment of the industry to learn
more about the liver abscess
                                                             Timing of treatment
                                                             Elanco’s research indicates that the
                                                             longer Tylan is fed to cattle and
                                                                                                                                    Being strategic
                                                                                                                                    With limited alternative options to
                                                                                                                                    this impactful disease, Tylan remains
disease complex and how to best                              the earlier Tylan is provided in                                       a cost-effective tool with a proven
manage it, the incidence of this                             the feeding period, the better liver                                   return on investment. Elanco’s
costly multifactorial condition is                           abscesses are controlled. A recent                                     research indicates that the earlier
not decreasing.1                                             study has shown that the voluntary                                     Tylan is provided in the feeding
                                                             removal of Tylan prior to slaughter                                    period and the longer it is fed to
                                                             may not impact its effectiveness in                                    cattle, the better liver abscesses are
With limited alternative options                             reducing liver abscess incidence at                                    controlled. Continued research is
to this impactful disease, Tylan                             slaughter (Table 1).1                                                  needed to better understand and
remains a cost-effective tool with                                                                                                  address this costly disease complex.
a proven return on investment.

                                                             TABLE 1.
A heavy cost to the bottom line
This multifactorial disease                                                               Effect of Strategic Use of Tylan
significantly affects the beef indus-                                                        on A+ Abscess Incidence
try through the loss of condemned
livers and other viscera because                                                      9
of reduced animal performance                                                         8   8.49
and carcass yield, and decreased
                                                                 Percent Abscesses

packer processing efficiency. Using                                                   7                                                                    7.36
conservative estimates, viscera                                                       6
losses are approximated at $60
million per year.1                                                                    5             5.67
                                                                                                                    4.75                                                  4.96
The effect of varying the Tylan                                                       4                                                  4.44
feeding schedule                                                                      3
                                                                                                                                          First 126 Days
                                                                                                    First 42 Days

                                                                                                                    First 84 Days

                                                                                                                                                           Last 84 Days
A study was conducted to determine
the effect of feeding duration and                                                    2
                                                                                          No Days

                                                                                                                                                                          All Days
timing on the efficacy of Tylan at                                                    1
reducing liver abscesses.1 Crossbred
steers (3,360 head) in the Texas                                                      0
Panhandle were divided into six                                                                     Tylan Treatment Group
treatment groups fed the same ration
for 164 days (February to August
2017). The average initial weight was
830 lbs, and final weights ranged
from 1,451 to 1,461 lbs.

Visit elanco.us or contact your Elanco sales representative to learn more about Tylan.

The label contains complete use information, including cautions and warnings. Always read, understand and follow
the label and use directions.
Tylan® (tylosin)
CAUTION: Federal law restricts medicated feed containing this veterinary feed directive (VFD) drug to use by or on the order of a licensed veterinarian.

For reduction of incidence of liver abscesses associated with Fusobacterium necrophorum and Arcanobacterium pyogenes: Feed
continuously as the sole ration at 8 to 10 g/ton of tylosin (90% DM basis) to provide 60 to 90 mg/hd/d.

Elanco supports the use of shared-class antibiotics for therapeutic uses while under the oversight of a veterinarian. Visit https://www.elanco.com/
healthy-purpose#responsible-use-of-antibiotics for more details on Elanco’s Antibiotic, Welfare and Sustainability Policies.
1
    Elanco Animal Health. Data on file in conjunction with the Ryan Travis Herrick dissertation.

Tylan, Elanco and the diagonal bar logo are trademarks of Elanco or its affiliates.
© 2020 Elanco.
PM-US-20-0595
CONTROLLING PATHOGENS ON DAIRIES WITH A WHOLE FARM APPROACH
writer//Tom Rehberger, Ph.D. / Director, Research and Product Development / Arm & Hammer Animal and Food Production

Harmful pathogens lurk everywhere on a dairy, leading         pneumonia. These illnesses compromise immunity and
to a cycle of infection and reinfection that hampers herd     take energy away from growth needs, leaving calves
health and performance. Taking a whole-farm approach          more susceptible to other illnesses. Infected calves can
to pathogen reduction can help you break this cycle and       also shed pathogens into the manure to heighten risk of
maintain your herd’s productivity.                            reinfection.

Controlling pathogens on your dairy starts by                 In the lactating herd, C. perfringens Type A is often
understanding the pathogen cycle. Pathogenic organisms        implicated in hemorrhagic bowel syndrome (HBS).
proliferate inside animals’ digestive systems and enter       Subclinical levels can cause cows to go off feed and
the environment through manure. These organisms often         reduce their milk production, even without signs of
survive in the manure pit or lagoon, with the potential       clinical disease.
to contaminate growing plants when manure is spread
on fields. When animals eventually consume the crop in        Left uncontrolled, the pathogen cycle continues through
the form of grazed or harvested forage, the pathogens         cows’ manure, which often contains clostridial bacteria,
return to their digestive systems and the cycle begins        including both toxigenic and non-toxigenic C. perfringens
anew, with damaging effects on health and productivity.       strains. Other clostridial strains can impact feed intake,
                                                              rumen efficiency and gastrointestinal function.
Pathogen prevalence
Three of the most prevalent pathogens on dairy farms are      Break the pathogen cycle
Clostridia, Salmonella and pathogenic E. coli. Realize that   To limit animals’ exposure to these and other pathogens,
your operation may be different from your neighbor’s,         your goal should be to decrease the overall pathogen
with different organisms present in the environment.          load on your dairy by breaking the pathogen cycle.
Assessing the existing pathogens can help identify a
solution for each area of your operation.                     One solution is feeding CERTILLUS™ to target specific
                                                              pathogenic challenges, control them and reduce
Since 2015, researchers at Arm & Hammer Animal and            reduce their levels in an animal. The Targeted Microbial
Food Production have analyzed clostridial risk on U.S.        Solutions™ in CERTILLUS use proprietary strains of
dairy operations. Among their findings1:                      Bacillus to help make calves and cows more resilient
• Over half of the 20,510 fecal samples tested from 509       against C. perfringens while reducing pathogen shedding
  dairies in 30 states fell into a high-risk category based   into the manure. ARM & HAMMER™ researchers select
  on levels of clostridial bacteria                           the best Bacillus strains to combat the specific challenges
• More than 70 percent of 1,200 calf fecal samples tested     in your herd by analyzing the Microbial Terroir™—which
  positive for pathogenic C. perfringens, with the highest    includes the environment, soil and animals at your
  prevalence in calves under three weeks old                  specific location.
• Forage samples from 457 herds revealed Clostridia
  presence in 83 percent of haylage and 57 percent of         Conclusion
  corn silage                                                 Although it is not possible nor desirable to create a
                                                              totally pathogen-free environment, proper management
Pathogen vulnerabilities                                      in all areas of the operation can help you reduce
Young calves are particularly vulnerable to environmental     risk of transmitting disease-causing organisms. Visit
pathogens. Exposure to pathogens during the first hours       AHfoodchain.com to learn more on the benefits of
and days of life can lead to clinical scours followed by      feeding CERTILLUS to dairy animals.

Notes
1 Data collected and analyzed by ARM & HAMMER.

                                                                                                                            13
NB Availa Sow                                    ®

  TM Plus

  A complete sow trace mineral with a blend of Zinpro Performance Minerals
  and inorganic minerals designed to maximize sow performance and improve                                          NUTRA
                                                                                                                   BLEND
  lifetime pig robustness. NB Availa®Sow TM Plus will meet your sow’s trace
  mineral needs with 50 ppm Zn, 20 ppm Mn, and 10 ppm Cu from Zinpro
  Performance Minerals in combination with inorganic mineral sources for
  complete mineral supplementation.

                                              Supplementing Availa®Sow Improves
                                                    Piglet Weaning Weight
                     Piglet Wean Weight, lb

                                                           Inorganic  Availa-Sow

  Feeding Availa-Sow in gestation and lactation can reduce somatic cell counts in colostrum and milk throughout lactation. Improved
  milk quality means better piglet performance on the sow. In five different studies, Availa-Sow improved weaning weight by an
  average of almost 0.5 pounds per piglet.

Contact your Zinpro or Nutra Blend representative today to find out more information.            nutrablend.net • zinpro.com
ZINPRO PERFORMANCE MINERALS®
REDUCE PRODUCTION COSTS AND INCREASE SOW LIVABILITY
writer //Zinpro Corporation

Minor changes can have major               scenarios.                                combination of lowering input costs
impacts when feeding Availa®Sow                                                      and increasing output. By lowering
throughout      gilt    development,       Have you considered the cost of           inflammation, Zinpro Performance
gestation and lactation. Sow               sow mortality in your system and its      Minerals re-direct more nutrients
mortality continues to be a challenge      impact on the cost of production?         towards a productive outcome, and
in the U.S. market with systems            What value could your system see if       on the sow farm the KPI that matters
commonly experiencing greater              you were able to lower sow mortality,     is cost per weaned pig.
than 10% sow mortality on average          and ultimately replacement rate by
(www.piglivability.org). Addressing        1 or 2%? Would you be willing to          By improving a sow’s ability to
sow mortality and the root causes          invest in strategies to help achieve      cope with immune pressure, more
of sow mortality to lower sow farm         this through nutrition, so your health    nutrients are available for the last
replacement rate simultaneously            and management strategies had             physiological priority, reproduction.
addresses cost of production; by           greater impact?                           A recent meta-analysis of Zinpro
lowering your replacement rate                                                       sow data revealed a 0.32 pigs per
you lower your cost of production.         Zinpro Performance Minerals® have         litter increase in born alive. While an
Cost per weaned pig is one of the          demonstrated not only to lower            increase in total born alive doesn’t’
three variables that explain 98% of        systemic inflammation in sows, but        guarantee and increase in pigs per
the variation system profitability.        also mitigate system mortality which      sow per year (PSY), adding effective
Effective trace mineral nutrition using    is ultimately what impacts cost of        trace mineral nutrition to your sow
Zinpro Performance Minerals is a tool      production. In recently completed         program is a means to improve
that can help you achieve a lower          field work, inclusion of Zinpro Availa-   management          opportunity      to
cost per weaned pig by lowering            Sow, part of the Nutra Blend Availa-      increase PSY and further lower cost
input costs through reduced sow            Sow TM Plus sow premix, resulted          per weaned pig.
mortality and increasing output with       in mitigation of sow mortality on a
increased born alive.                      system level (Figure 1).                        The inclusion of Availa-Sow in
                                                                                           the sow’s diet provides zinc,
Inflammation        impacts     Figure 1                                                   manganese and copper from
nutrient         utilization,                                                              performance trace minerals.
physiological        priority                                                              By making a minor change to
and system profitability.                                                                  the sow’s diet and including
More than 50% of sow                                                                       Zinpro Performance Minerals®
mortality is attributed                                                                    from Availa-Sow or the Nutra
to “unknown” reasons                                                                       Blend Availa-SowTM Plus sow
(www.piglivability.org),                                                                   premix, a major impact can be
and while it’s real that                                                                   achieved on cost per weaned
we don’t know why many                                                                     pig directly linked to sow
sows die, it’s reasonable                                                                  performance.
that inflammation is at
the core of the problem.
Inflammation is necessary
for     positive      health
outcomes. It alerts the
immune system to challenges; when                                                    To learn
left uncontrolled it can rob animals of    Past research has indicated that          m o r e
necessary fuel for production and, in      Zinpro technologies improve the           about
extreme conditions, result in natural      robustness of the feet and the gut,       including
death or euthanasia, impacting             ultimately leading to optimal health      Availa-
system      profitability.    Persistent   outcomes and survivability.1,2 By         Sow     in
immune responses divert nutrients          lowering sow mortality, reductions in     your swine feeding and nutrition
away from measurable KPIs and              replacement rate can be recognized        program, contact your Nutra Blend
ultimately impact system profitability     leading to a lower cost per weaned        account manager or a Zinpro Swine
in the form of involuntary culls,          pig as a result of fewer gilt entries.    account manager today.
re- treats or other profit robbing         Reducing cost of production is a

References
1
  Leite, F.L. et al., 2018
2
  Anil, S.S et al., 2010
                                                                                                                               15
Her Biology.
    Our Technology.
    Smart science brings us more than data and devices.
    It delivers the industry’s most effective immune
    support product — NutriTek®.

    Working naturally* with the cow’s biology, NutriTek
    helps maintain immune strength for optimal health
    and more quality milk.

    Healthy herd. Total dairy performance.

    Life Stage Solutions®.
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    *natural as defined by AAFCO

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For more information, visit www.diamondv.com/nutritek
MANAGEMENT TIP TOPIC: BUILD A HEALTHY RUMEN ENVIRONMENT
writer //Diamond V

The health of your herd starts               the mix is critical. The end goal is to    as well as the cow. Remember -
with good rumen management.                  meet the required nutrient profile.        when we think about the microbial
Healthy cows perform better, and                                                        community and the contribution to
better performance can lead to               Encourage the Growth of Beneficial         the cow, it is about the protein and
greater production and ultimately            Rumen Microbes:                            energy supplies. Anything we do that
greater profitability. Without a well-       A     nutritionally   balanced      diet   disrupts the balance of the numerous
functioning rumen, your herd could be        is important as it provides an             rumen microbial populations can
more susceptible to a variety of health      environment that maximizes growth          influence how well the cow is able to
complications and costly expenses.           and activity of the rumen microbial        utilize the nutrients that we are putting
Two things are vital when it comes to        community. Through fermentation of         in front of her.
optimal rumen health: a stable, active,      different ration components inside the
and healthy microbial population;            rumen, microbes work to break down         One way to give the rumen microbes
and maintaining a suitable rumen             what a cow eats, producing volatile        what they need to grow is incorporating
pH level.. Without these two things          fatty acids (VFA) which the cow utilizes   appropriate feed additives in the
in place, the rumen becomes less             for its energy needs. Also, rumen          cow’s diet. NutriTek® is Diamond V’s
efficient at digesting feed.                 microbes utilize non-protein nitrogen      most advanced natural* nutritional
                                             (NPN) to synthesize microbial protein,     health product for all dairy animals.
Incorporate the three management             which becomes available to the             Diamond V has been in the animal
tips listed below to build a healthy         cow. It’s all about providing the right    feed additives business for more than
rumen environment.                           nutrients to the right populations to      75 years. Our natural* products are
                                             produce the most beneficial levels of      made from a proprietary fermentation
Create the Right Balance in the              volatile fatty acids (VFAs) The three      process that helps optimize animal
Ration:                                      main VFAs are:                             health, animal performance, immune
A healthy rumen starts with a well-          • Acetate: This is the end product of      support, and food safety worldwide.
balanced ration. The right balance             fiber fermentation. It’s necessary       NutriTek can help support better gut
of nutrients ensures micro-organisms           for milk fat, so too little acetate      health, facilitate balanced immune
work effectively, and the cow can              production creates a lower fat test.     function, and ultimately aid in
obtain the greatest nutritional benefit      • Propionate: This is the result of        increased production and profitability.
from the feed. A consistent and                fermentation of starch and sugars.       A cow with a healthy rumen is able to
constant supply of nutrients is key for        This provides the cow with energy for    digest feed more efficiently and make
optimal rumen pH and rumen microbe             weight gain and lactose production       more energy and nutrients available
growth and activity.                           inside the udder. If propionate          for milk production and other bodily
                                               levels are reduced, as they can be       functions. The bioactives in NutriTek
However, it’s all a balancing act with the     in high-fiber diets, milk production     work synergistically to help balance
end goal of having each mouthful of            can suffer.                              immunity and optimize digestive
feed be as close to complete nutrient        • Butyrate: This is metabolized in the     health by promoting a healthy rumen
profile for the cow. When building a           liver into ketone bodies, which are      microbiome and delivering beneficial
diet, focus on the nutrients critical          used as a source of energy for fatty     antioxidant effects. Creating the right
to a cow’s daily needs like vitamins,          acid synthesis and the development       balance in the ration, establishing
minerals, carbohydrates (starch, fiber),       of other body tissues.                   beneficial VFA production, and
fats, protein, and water. It is important                                               feeding a product that favorably
to understand how these components           Feed Products That Give Rumen              impacts the rumen microbes can
interact and their influence is, to rumen    Microbes What They Need to Grow:           ultimately improve gut health and
microbial function. The other thing to       Formulating the precise nutrient           immune strength. The result will be
keep in mind is feed sorting. Cows will      requirements in the diet can be a          increased feed intake, improved
sort feed based on preference and            challenge. We must build a diet that       efficiency, and more milk production.
particle size; therefore, uniformity in      feeds both the microbial community
                                                                                                                              17
BALCHEM RESEARCH SUMMARY:
STUDY MEASURES METHYL DONOR CONTENT OF KEY COMMODITIES
writers // Kari Estes1, Barbara Barton1, Ryan N. Dilger2, and Thomas Powell1
1
 Balchem Corp., New Hampton, New York, United States
2
 Department of Animal Sciences, University of Illinois, Urbana, Illinois, United States.

Purpose                                                          Conclusions
The goal of this project was to sample and analyze               Accurately characterizing the choline content in
numerous soybean meal (SBM) and corn samples                     commodities has important implications. Using the
collected in 2018 for choline and other methyl-                  Poultry NRC (1994) values to formulate for choline
containing compound concentrations to build an                   could cause a gross under-supplementation in most
updated library.                                                 diets. Table 2 compares the supplementation rates
                                                                 needed to meet dietary choline requirements when
Methods                                                          NRC book values and analyzed values are used to
Twenty-nine SBM samples and 22 corn samples were                 formulate a typical (corn/soy based) broiler starter
collected from two international countries and 10                diet.
states within the United States. Samples were then
analyzed at Eurofins Scientific (Des Moines, Iowa) for           This discrepancy, coupled with a potentially greater
amino acids, choline, betaine, folic acid, and vitamin           choline requirement to meet the needs of today’s
B12 concentrations.                                              genetically superior production animals, could
                                                                 seriously compromise productivity and producer
                                                                 profitability.

Results
• In general, corn exhibited lower analyzed methyl-
  containing nutrient concentrations and was more
  variable when compared with SBM (Table 1).
• Vitamin B12 concentrations in both SBM and corn
  were low and below detection limits (
Horn fly season is winding down, but a “do nothing” approach could give flies a
                                                                                                  head start on next season. Keep Altosid® IGR in your feed supplement until
                                                                                                      30 days after the first frost to prevent overwintering. Bring your horn fly
                                                                                                               program full circle for higher production and better protection.

                                                                                                                                To learn more, visit AltosidIGR.com or talk to your
                                                                                                                                                              supplement provider.

                                                                                                                                                                       ®

                                                                                                                                                                                  ®

Always read and follow label directions. Altosid and the cow head design are registered trademarks of Wellmark International.
                                                                                                                                                                           19
Central Life Sciences with design is a registered trademark of Central Garden & Pet Company. ©2017 Wellmark International.
DON’T LET MYCOTOXINS TANK THE PROFITABILITY OF YOUR DAIRY FARM
writer // Dr. Rafael Caputo Oliveira

Mycotoxins are toxic compounds produced by molds                                   brewer yeast selected among other strains by
that, if not well managed, can silently reduce a                                   researchers at Ghent University in Belgium to have
dairy farms profitability by reducing feed intake and                              the highest immunomodulation activity. Our most
milk production, increasing diseases, and reducing                                 advanced formula also contains antioxidants and
reproductive performance. In a mycotoxin survey                                    botanicals that help alleviate and repair the cellular
with feed samples from farms throughout the United                                 damage caused by mycotoxins in dairy cows.
States in 2019 (Borutova, 2019), 4.5 to 50% of all corn
and silages were contaminated with zearalenone and                                 Is Adisseo MMS efficacy scientifically proven?
fumonisin, and 86.4 to 100% of all feed samples were                               Researchers at the University of New Hampshire
contaminated with DON.                                                             carried out an experiment to study the capacity of
                                                                                   Adisseo MMS on ameliorating the negative effects
Screening and, if necessary, eliminating feedstuffs                                of mycotoxin-contaminated distiller grains in dairy
are key to have low dietary levels of mycotoxin. In                                cows (Whitehouse., et al 2020). The experiment was
partnership with global players, Adisseo offers a                                  composed by three dietary treatments:
certified, user-friendly, and rapid test for specific                              1. Healthy diet
mycotoxins. For a complete screening of mycotoxins,                                2. Diet containing mycotoxin-contaminated distiller
Adisseo also offers through third-party labs wet                                      grains
chemistry mycotoxin analysis using LC/MS-MS.                                       3. Diet containing mycotoxin-contaminated distiller
                                                                                      grains and Adisseo MMS.
Eliminating contaminated feedstuffs is the best
solution, but in some circumstances due to inventory                               The distiller grains used in this experiment were
issues this recommendation is not practical. In those                              naturally contaminated with high levels of the
cases, once the feedstuff is contaminated with                                     most prevalent mycotoxins in the United States
mycotoxins, the most feasible solution is the use of                               (zearalenone, fumonisin B1, and DON).
dietary additives to prevent and mitigate the impact
of mycotoxins and improve dairy profitability.                                     As expected, cows fed the diet containing
                                                                                   contaminated distiller grains had reduced dry matter
Once you are equipped with a mycotoxin screening                                   intake and energy corrected milk in 4.4 and 2.9 lbs/
analysis from single ingredients or TMR, we                                        day, respectively, and had impaired immune function.
recommend using the app MycoMan® that can be
downloaded for free from the Apple Store (iOS) and                                 Cows fed the diet containing contaminated distiller
the Google Play Store (Android). This app helps you                                grains and Adisseo MMS had a less severe negative
to diagnose a potential mycotoxin problem and                                      impact on dry matter intake (-2.6 lbs/day) and
define the best strategy to prevent or control possible                            interestingly, immune function was similar to cows
diminished dairy performance by suggesting the type                                consuming the healthy diet.
of products and their doses.
                                                                                   It is also worth noting the Adisseo MMS-fed cows
Preventing dairy cows from mycotoxin poisoning                                     started the trial with the lowest energy corrected milk
does not need to be expensive; Adisseo offers two                                  production but by the end of it, they had the highest
mycotoxins management solutions (MMS) that can                                     production.
cost as low as 2 cents daily per cow. Our products
prevent and control a wide range of mycotoxins                                     As a conclusion, these results prove the efficacy
harmful effects through detoxifying and repairing                                  of Adisseo MMS in protecting dairy cows from the
modes of action. All Adisseo MMS contain a high-                                   harmful effects of the most prevalent mycotoxins in
quality bentonite (97% affinity to aflatoxin; Trilogy                              the United States
Analytical Lab. certificate) and a high-quality dried

Sources
Borutova, R. 2019. Adisseo: United States of America 2019 Mycotoxin Survey in Corn and Corn Silage.
Whitehouse, N. L., H. C. Robertson, B. K. Kerns, S. M. Hollister, L. H. P. Silva, and S. M. Fredin. 2020. Effects of supplemental mycotoxin deactivator on
lactation performance of Holstein cows fed natural mixed mycotoxin contaminated feed. J. Dairy Sci. 103 (Suppl. 1):125. (Abstr.)

20
20 SEPTEMBER
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20                         CUSTOMER REWARDS POINTS
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                                                                                                                                    CONTINUE TO PAGE 22
LINCOMIX®: NOW APPROVED
                                                                                                                     AT 100 TO 200 GRAMS PER TON

                                                                                                                     LINCOMIX® (lincomycin) from Zoetis now has a broader
                                                                                                                     dosage range to provide pork veterinarians and producers
                                                                                                                     with greater flexibility in reducing Mycoplasma hyopneumoniae
                                                                                                                     challenges. See how LINCOMIX — the only Type A Medicated
                                                                                                                     Article with a claim associated with M. hyo management —
                                                                                                                     can improve health and economic outcomes at
                                                                                                                     zoetisUS.com/LINCOMIX.

                                                                                                                     CAUTION: Federal law restricts medicated feed containing
                                                                                                                     this veterinary feed directive (VFD) drug to use by or on
                                                                                                                     the order of a licensed veterinarian.

 All trademarks are the property of Zoetis Services LLC or a related company or a licensor unless otherwise noted.
 © 2018 Zoetis Services LLC. All rights reserved. LIN-00036

  NB September Points
  CONTINUE 3
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  DRY
Job
Bill to
            APEX®
             ZOPKFEED7058
             ZOPK8FEED052
                           CALF.............................................
                                                         Producer
                                                         CD
                                                                                      20/#
                                                                               Maureen C.
                                                                               Sarah \(for Danielle\
                                                                                                       DRY
                                                                                                     Fonts
                                                                                                                 SPECIAL              ANISE             FLAVOR......................
                                                                                                     Gotham Narrow (Black, Book, Book Italic, Medium), Gotham Condensed (Book)
                                                                                                                                                                                                    20/#
  DRY APEX®
Client       ZOETIS        POULTRY..................................... 20/# Images
                                                         AD                    N/A                     DRY STRAWBERRY 5000.............................. 20/#
  DRY       APPLE
Media Type Newsletter
                          CINNAMON           PELLET-ADE. Copywriter
                                                                            . .....
                                                                               N/A
                                                         Production Artist Meg W.
                                                                                      20/#             DRY ULTRACID(CMYK;
                                                                                                     4CB13ALS_PAH_D1L1_0013_RT.tif         P..481
                                                                                                                                                .............................................       20/#
                                                                                                                                                   ppi, -482 ppi; 62.31%, -62.31%), MFA_Stacked_Graphic_White.

  DRY APPLE-ADE................................................ 20/#                                   DRY VANILLA-ADE........................................... 20/#
                                                                                                     eps (90%), MFA_Stacked_Graphic.eps (90%), Zoetis_logo_0k.eps (13.24%), Lincomix_WHT.eps (21.93%)
                                                         Proofreader           Alex L.

  DRY
Live
            APPLE-ADE
             7.5” x 4.875”
                               PELLET         FLAVOR.               . ............    20/#             DRY       WINTERGREEN........................................                                20/#
                                                         Account               Ken Ryan              Inks
Trim         8.5” x 5.5”                                                                                  Cyan,   Magenta,    Yellow,       Black
  DRY CALF-ADE.................................................. 20/#
Bleed        8.625”  x 5.625”              Notes                                                       EB-LYS...................................................................175/#
  DRY       CARAMEL-ADE........................................                       20/#             EB-MET.................................................................175/#
                                           Keep new files under old job number, just bill-to 2018
Due To Pub 4/20                            number.

  DRY CHERRY-ADE............................................ 20/#                                      Eco-Gest YS® Blend Down............................... 5/#
  DRY CREAM-SICLE FLAVOR......................... 20/#                                                 ECO-GEST-YS ® .................................................10/#
  DRY DAIRY-KRAVE®.......................................... 20/#                                      Feed CURB® Dry................................................30/#
  DRY DAIRY-KRAVE® 400................................ 20/#                                            Fresh CUT® Plus Liquid....................................40/#
  DRY EVERGREEN BC FLAVOR..................... 20/#                                                    HEIFER MAX 500® Liquid.............................. 50/#
  DRY GARLIC-ADE............................................. 20/#                                     HEMICELL 1.5X................................................... 50/#
  DRY HAY-ADE.................................................... 20/#                                 HILYSES-THE BIONIC PREBIOTIC............... 20/#
  DRY LYSOPEX.................................................... 20/#                                 KALLSIL™ .............................................................. 60/#
  DRY MAXI-SWEET US..................................... 20/#                                          Kem Lac® HD.....................................................100/#
  DRY MOLASSES-ADE...................................... 20/#                                          KemTRACE® Chromium 0.4% Dry.................75/#
  22
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