PROGRESS REPORT 2020 Interdisciplinary Center for Clinical Research of the RWTH Aachen Faculty of Medicine
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PROGRESS REPORT 2020 Interdisciplinary Center for Clinical Research of the RWTH Aachen Faculty of Medicine
Progress Report IZKF Aachen 2020
Preface Preface
Prof. Dr. Peter Walter (Speaker of the IZKF)
Dear Colleagues and Friends,
Our world has changed dramatically since last year, and we all are facing new challenges well-functioning and supportive unit, helping many researchers at our faculty to generate
after the appearance of the SARS CoV2 virus. This is the second annual report of IZKF important data from their experiments.
Aachen during the coronavirus pandemic, and although we have learned so many facts In 2020 we also established some new technologies within the IZKF units, such as the
about the virus and its disease and although vaccines were developed in record-breaking CellenOne Single Cell Analysis in the Genomics Facility, fluorescence lifetime imaging
time, there are still many restrictions in place that negatively impact scientific and clinical in the Two-Photon Imaging Facility, and the CRISPR/Cas technology in the Transgenic
work as well as social life. Many researchers are still working in home offices together Service Facility.
with their children who cannot attend classes in person. Our social life is limited to
videoconferences. We cannot visit our parents or grandparents because we must prevent Also in 2020, the terms of some of the members of the IZKF Steering Committee came to
transmission of the virus to those at risk. International travel, in-person conversations, and an end and new colleagues joined the board. In addition, the speaker’s term ended and the
meetings with friends and colleagues are not possible. Even those of us who have been Medical Faculty decided that Professor René Tolba, Professor Marc Spehr and I would be
strictly following the rules, convinced that it is the right thing to do, are getting tired after allowed to continue with our work for IZKF Aachen.
more than a year of physical distancing.
Throughout this difficult time the staff of the IZKF administrative office, Karen De Bruyne,
But life is not standing still. Somehow things are moving forward and progress is being Sünje Frahm and Sandra Majewski, did a fantastic job to keep the wheels turning and to
made. IZKF Aachen has funded two new collaborative projects: Professor Lampert find creative solutions even when the road was blocked and the exit was not visible. I am
is managing the project on Sodium channel-related pain disorders, representing the deeply thankful.
Neuroscience focus of the Medical Faculty, and Professor Wolf is leading the project on
Regulatory networks controlling the soft tissue-alveolar bone crosstalk in periodontal It is my sincere hope and strong wish that my next report, describing IZKF Aachen’s
disease, representing the Organ Crosstalk focus. achievements in 2021, will start with something like… “It is over…”
IZKF Aachen is now running eight core facilities. Each of the core facilities was successfully Fingers crossed, take care, and stay healthy.
reviewed by several external experts, making it possible to continue their funding. However, Yours faithfully,
organizational changes were necessary in the Genomics Facility, and a new concept was
developed by Professor Ingo Kurth. This concept is now under evaluation to prepare
for a new funding period. When mentioning the Genomics Facility at this point, it is also
my sad, sad duty to announce that Dr. Bernd Denecke, the core facility manager of the
Genomics Facility, a part of IZKF right from the beginning, passed away after suffering from Professor Dr. Peter Walter
a serious illness. He developed the Genomics Facility from the early days of the IZKF into a on behalf of the Steering Committee of IZKF Aachen
4 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 5
Imprint Contents
Contents
Preface p. 4
Imprint p. 6
1. Annual Report p. 8
1.1 The IZKF in 2020 p. 8
1.2 Funding Measures in a Nutshell p. 11
1.3 Funding and Financial Overview p. 12
1.4 Participating Institutes and Clinics p. 14
1.5 Output and Evaluation p. 17
2. Function and Goals p. 18
3. Core Facilities p. 20
3.1 Using Institutes and Clinics p. 22
Progress Report 2020 3.2 Core Laboratory p. 26
3.3 Genomics Facility p. 29
3.4 Immunohistochemistry Facility p. 32
Publisher 3.5 Confocal Microscopy Facility p. 34
Interdisciplinary Centre of Clinical Research (IZKF Aachen) 3.6 Brain Imaging Facility p. 37
Speaker: Prof. Peter Walter 3.7 Two-Photon Imaging Facility p. 43
Pauwelsstraße 30 3.8 Transgenic Service p. 48
52074 Aachen 3.9 Proteomics Facility p. 52
3.10 Flow Cytometry Facility p. 56
Phone: +49 241 80 80034
Email: izkf@ukaachen.de
www.izkf-aachen.de 4. Projects p. 60
Thematically based on the Main Research Focus Areas of the Faculty of Medicine
Editor 4.1 Phase Transition in Disease p. 60
Karen De Bruyne M.A. (administration office) 4.2 Translational Neurosciences p. 94
4.3 Organ Crosstalk p. 120
Layout
Nicole Miehlke, Stolberg
5. Research Groups p. 138
Cover 5.1 J. Di Russo: Retinal Epithelium Mechanobiology and Disease p. 140
Peter Winandy, Aachen 5.2 E. van der Vorst: Immune-Lipid Crosstalk p. 143
5.3 B. Namer: Different functional roles of nociceptor subclasses in human p. 146
Printing
frank druck+medien GmbH & Co. KG, Aachen
Appendix p. 152
Print Run
180 copies Articles of Association
Aachen, April 2021
The project leaders are responsible for the content of their reports and for the
information on their external funding, publications, etc.
6 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 7
1.1 | The IZKF in 2020 1.1 | The IZKF in 2020
The Interdisciplinary Center for Clinical
The hiring of Dr. Poznansky, a physicist with significant experience in developing MR
sequences, will greatly improve the quality of methodological services in the Brain Imaging
Facility.
Research (IZKF) in 2020 One very pleasing development was the approval of investment funds from the DFG to
purchase a multiphoton laser scanning microscope with FLIM for the Two-Photon Imaging
Start of two Joint Research Project Proposals (2020-2023) Facility. Thanks to this approval, the facility will boast state-of-the-art equipment in 2021.
On the basis of the external review process of the previous year the Steering Committee Prof. Tolba, Dr. Vogt, Prof. van Zandvoort and Prof. Martin were responsible for the
unanimously decided to fund the following two joint projects for three years: successful grant application.
With the hiring of Dr. Kapsokalyvas, we were able to expand our methodological expertise
in the area of highly developed light microscopes, especially in the area of in vivo imaging in
Funding start Joint Research Project Research Focus Area Speaker small animals and fluorescence lifetime imaging.
1.3.2020 Sodium channel-related pain disorders Translational Neurosciences Prof. A. Lampert
We were also very pleased about the good news that the funding application for an
1.11.2020 Regulatory networks controlling the soft tissue- Organ Crosstalk Prof. M. Wolf investment in a 5-laser cell sorter for the Flow Cytometry Facility, organized by Prof. Pabst,
alveolar bone crosstalk in periodontal disease was approved by the DFG. This high-end sorter will allow us to sort human cells and
undertake important research projects.
This further development of our equipment and methodology would not have been possible
External evaluation process and further funding for existing Core Facilities without the department’s active support, since the IZKF budget does not cover the
The Core Facilities were evaluated by two external reviewers with experience in the Core financing to acquire research equipment.
Facility management. The comprehensive, individual reviews contained constructive The new large research devices will be put into operation throughout the year in 2021.
criticism with suggestions for improvement, and also certified the facility‘s technical
equipment with a good to very good rating. After the external reviews, the steering Election of the Steering Committee
committee confirmed further funding for the Core Facilities for another three years The Speakers for the faculty research focus areas were requested to nominate members for
according to their applications. The significance of the central services for faculty research the new IZKF Steering Committee. A Focus Area Coordinator and Deputy were proposed
was reaffirmed: It is increasingly important to promote cooperation between groups within for each research focus area. Focus Area Coordinators do not receive IZKF funding
the faculty which make use of the same equipment and techniques. themselves.
Restructuring of the Genomics Facility Core Facility Managers were also requested to select a Deputy from among their colleagues
During the last year the Genomics Facility faced substantial managing difficulties. The Core to be assigned to the Steering Committee. Prof. Müller-Newen was re-elected as the Core
Facility has been led provisionally by Prof. Ingo Kurth, Director of the Institute of Human Facilities Representative.
Genetics. The scientific position and one technical position in the wet-lab remained vacant
for a very long time. Due to an unclear perspective of the Facility the Steering Committee
decided to postpone the external evaluation for a year, in order to rethink the direction
of the Facility within the faculty. After intense discussions by the faculty and the users,
the importance of Next Generation Sequencing technologies was emphasized and a
restructuring of the Genomics Facility has been decided. In order to establish continuity and
to make use of the expertise of human genetics, Prof. Kurth is to be appointed continuously
as head of the Genomics Facility. The external evaluation is taking place in 2021.
Further development of equipment and methodology in the Core Facilities
In the past year, the Core Facilities have seen very positive further development thanks
to the hiring of important methodology experts and the prospect of acquiring advanced
equipment.
The Genomics Facility has purchased the CellenOne F4.1 System, that can be used in
combination with the iCell8 system (Takara) which is already available at the faculty. The
Genomics Facility will now be able to cover the entire methodical spectrum of the genome
wide single cell analyses.
Dr. Marks joined Transgenic Service as an experienced expert who can establish the high-
demand CRISPR/Cas9 technology on site.
8 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 9
1.1 | The IZKF in 2020 1.2 | Funding Measures in a Nutshell
Funding Measures in a Nutshell
The newly elected Steering Committee consists of:
Prof. Dr. P. Walter IZKF Speaker
Project Funding
Prof. Dr. R. Tolba IZKF Deputy Speaker
35 projects
Prof. Ruth Knüchel-Clarke Focus Area Coordinator for Medicine Technology & Digital Life Sciences
(40 % of the total expenses during the report period)
Prof. Dr. N. Marx Focus Area Coordinator for Organ Crosstalk
Prof. Irene Neuner Focus Area Coordinator for Translational Neurosciences Research Groups
Prof. Dr. M. Hornef Focus Area Coordinator for Phase Transition in Disease 3 Research Groups
Prof. Dr. M. Spehr Representative of the Faculties of Engineering and Natural Sciences (14 % of the total expenses during the report period)
Prof. Dr. G. Müller-Newen Core Facilities Representative
Core Facilities / Laboratory
Prof. Dr. Julia C. Stingl Deputy Focus Area Coordinator for Medicine and Technology
Core Laboratory
Prof. Dr. O. Pabst Deputy Focus Area Coordinator for Organ Crosstalk/ Speaker SFB 1382
Genomics Facility
Prof. Dr. J. Schulz Deputy Focus Area Coordinator for Translational Neurosciences
Immunohistochemistry Facility
Prof. Dr. E. Stickeler Deputy Focus Area Coordinator for Phase Transition in Disease
Confocal Microscopy Facility
Prof. Dr. S. Uhlig Dean of the Faculty of Medicine
Brain Imaging Facility
Prof. Dr. C. Trautwein Speaker TRR (guest member of the Steering Committee 2009-2020)
Two-Photon Imaging Facility
Prof. Dr. J. Jankowski Speaker SFB/TRR219 (guest member of the Steering Committee 2018-2021)
Transgenic Service
Proteomics Facility
Election of the Speaker and the Deputy Speaker of the IZKF by the Faculty Board, Flow Cytometry Facility
December 7th
During the faculty meeting on December 7th, Prof. Peter Walter was re-elected as the
Speaker of the IZKF by the Faculty Board for another three-year period. Prof. René
Tolba was re-elected as the Deputy Speaker for the next three years. Prof. Spehr was (42 % of the total expenses during the report period)
unanimously re-elected as the Representative of the Faculties of Engineering and Natural
Sciences.
4 % of the total expenses during the report period was spent for the scientific coordinating office and central costs.
10 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 11
1.3 | Funding and Financial Overview 1.3 | Funding and Financial Overview
Funding and Financial Overview
Research Groups
In 2020, 40 % of the overall expenses of € 582,906
the IZKF was spent on project funding, 14 %
14 % on funding research groups and 41 % Project Funding
In 2020 the IZKF received € 3,175,380 from the State Grant for Research and Teaching as on core facilities. A further 4 % was spent € 1,589,733
fixed in the faculty budget. It also received a faculty grant of € 1,227,289. The total budget on the administration office and 1 % on the 40 %
amounted to € 4,402,669. IZKF core laboratory.
Core Facilities
€ 1,670,024
Budget and Expenses 2020 41 % Administration Office
€ 151,080
Budget 2020 € 4,402,669 4%
Core Laboratory
Total Expenses 2020 € 4,026,682 € 32,939
1%
Projects € 1,589,733 Spreading of Expenses € 4,026,682
Phase Transition in Disease € 757,282
Translational Neurosciences (from 01.03.2020) € 649,459
Organ Crosstalk (from 01.11.2020) € 131,329
Organ Crosstalk, Single Project € 51,663
Flow Cytometry Facility
Research Groups € 582,906 Proteomics Facility
€ 117,286
Immune-Lipid Crosstalk (van der Vorst) € 191,717 € 214,796
7%
Roles of Nociceptor Subclasses (Namer) € 189,812 Core Facilities were funded with 13 %
Genomics Facility
Retinal Epithelium Mechanobiology and Disease (Di Russo) € 201,377 € 1,670,024 in total.
€ 294,400
Two-Photon Imaging Facility
18 %
€ 177,398
Core Facilities € 1,670,024 10 % Immunohistochemistry
Genomics Facility € 294,400 Facility
Immunohistochemistry Facility € 227,631 € 227,631
Transgenic Service
Confocal Microscopy Facility € 75,270 14 %
€ 132,625
Brain Imaging Facility € 430,618 8%
Transgenic Service € 132,625 Confocal Microscopy Facility
Two-Photon Imaging Facility € 177,398 € 75,270
Proteomics Facility € 214,796 Brain Imaging Facility 4%
Flow Cytometry Facility € 117,286 € 430,618
26 %
Administration Office and Core Laboratory € 184,019
Administration Office € 151,080
Core Laboratory € 32,939
Funding Core Facilities € 1,670,024
12 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 13
1.4 | Participating Institutes and Clinics 1.4 | Participating Institutes and Clinics
Participating Institutes and Clinics
Teaching and Research Area Experimental Neuropsychiatry
Teaching and Research Area Psychopathology
Teaching and Research Area Experimental Behavioral Psychobiology
Teaching and Research Area Structural Functional Brain Mapping
Clinical Departments 2020
Teaching and Research Area Structure of Cortical Functional Units
Department of Anaesthesiology
Teaching and Research Area Functionality of Cortical Circuits
Internal Medicine I
Teaching and Research Area Neuropsychological Gender Studies
Department of Cardiac and Thorax Surgery
Department of Oral Maxillofacial and Plastic Facial Surgery X
Teaching and Research Area Child Cardiac Surgery
Department of Radiotherapy
Department of Child and Adolescent Psychiatry
Department of Surgery
Teaching and Research Area Clinical Child and Adolescent Neuropsychology
Department of Urology
Department of Dental Preservation
Department of Vascular Surgery
Teaching and Research Area Oral Microbiology and Immunology X
Internal Medicine II X
Department of Dermatology
Internal Medicine III X
Department of Diagn. and Interv. Radiology
Internal Medicine IV X
Department of Diagn. and Interv. Neuroradiology
Internal Medicine V
Department of General, Visceral and Transplant Surgery
Internal Medicine VI
Department of Gynaecology and Obstetrics
Surgical Intensive Care
Teaching and Research Area Prenatal Medicine
Institutes
Department of Gynaecological Endocrinology and Reproductive Medicine
Institute of Aerospace Medicine
Department of Neurosurgery
Institute of Applied Microbiology
Department of Neurology X
Institute of Anatomy and Cell Biology X
Teaching and Research Area Clinical Cognition
Institute of Biochemistry and Molecular Biology X
Teaching and Research Area Neuropsychology
Institute of Biochemistry and Molecular Cell Biology
Department of Nuclear Medicine X
Institute for Biochemistry and Molecular Immunology
Department of Ophthalmology
Helmholtz-Institute for Biomedical Engineering
Teaching and Research Area Experimental Ophthalmology (Applied Medical Engineering)
Department of Orthodontics X Teaching and Research Area Rehabilitation and Prevention Engineering
Department of Orthopaedics X Teaching and Research Area Cardiovascular Engineering
Department of Trauma Surgery X Teaching and Research Area Tissue Engineering and Textile Implants
Department of Otorhinolaryngology, Plastic Head, Neck Surgery Helmholtz-Institute for Biomedical Engineering X
Department of Paediatric Medicine X (Experimental Molecular Imaging)
Teaching and Research Area Neonatological Intensive Care Helmholtz-Institute for Biomedical Engineering (Cell Biology) X
Department of Paediatric Cardiology Teaching and Research Area Stem Cell Biology X
Department of Palliative Medicine X Helmholtz-Institute for Biomedical Engineering (Biointerface) X
Department of Phoniatrics, Pedaudiology and Communication Disorders Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry X
Department of Plastic, Hand and Burns Surgery X Institute for Computational Genomics X
Department of Prosthodontics and Dental Materials X Institute of History, Theory and Ethics in Medicine
Teaching and Research Area Dental Materials and Biomaterials Research X Institute of Human Genetics X
Department of Psychiatry X Institute for Hygiene and Environmental Medicine
14 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 15
1.4 | Participating Institutes and Clinics 1.5. | Output and Evaluation
Output and Evaluation
Institute of Immunology
Institute of Laboratory Animal Science
Institute of Medical Psychology und Medical Sociology
Institute of Medical Informatics 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020
Institute of Medical Statistics Publications* Articles 60 81 49 61 101 73 147 93 144 170 140 67 114 118
Institute of Medical Microbiology Scientific Diploma
degrees** theses
Teaching and Research Area Virology 6 3 7 10 15 8 18 13 14 26 19 7 4 2
Bachelor/
Institute of Molecular and Cellular Anatomy Master
Institute for Molecular Cardiovascular Research X Doctoral
5 12 9 9 15 10 12 15 13 14 28 25 4 9
Institute of Molecular Medicine theses
Institute of Neuroanatomy Postdoctoral
Lecture 2 0 3 4 3 2 1 - - - 3 0 1 0
Institute of Neuropathology
Qualification
Institute of Pharmacology and Toxicology X
Teaching and Research Area Pharmacology
Teaching and Research Area Pharmacology of Inflammation
Institute of Pathology X
Teaching and Research Area Tumour Pathology
Institute of Physiology X
Institute of Medical Psychology and Medical Sociology
External Cooporations
DWI – Leibniz Institute for Interactive Materials
AICES - Aachen Institute for Advanced Study in Computational Engineering Science
Biology II, Faculty 1, RWTH Aachen University X
* IZKF support must be mentioned in publications and presentations. Publications are relevant to the IZKF if the support is
mentioned in the acknowledgement and/or in the affiliation.
The number of publications fluctuates year to year and depends on which stage the project is in during the funding period.
** Information about diploma theses and doctoral theses can be found in the project reports.
16 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 17
2 | General Information | Function and Goals 2 | General Information | Function and Goals
Function and Goals of the IZKF
Joint Projects
Thematically directed towards the faculty‘s research focus areas The IZKF places utmost
importance on funding high-quality innovative joint research projects. All project applications
are subject to an external peer review process, that guarantees the credibility of the
underlying science and fosters a transparent and verifiable allocation of funds. The review
process results in a high level of acceptance among the applicants and in the faculty
of medicine. Furthermore, the funded projects are subject to a result-oriented internal
evaluation.
Research Groups
Group management for young, excellent researchers The IZKF offers an attractive funding
possibility for scientists with outstanding achievements through its research groups: The
research groups are assigned to an institute or department and independently work
there. The heads of the groups possess excellent scientific qualifications and experience
in acquiring external funding. They are as well externally reviewed on the basis of their
scientific excellence, strategical fitting accuracy and their potential to strengthen the
faculty´s research focus areas.
Core Facilities
Technologies, equipment and expertise for ambitious research goals Making research
infrastructure available to the community presents an enormous challenge to both facility
managers and administration. The faculty-wide platforms overcome institutional boundaries,
adhere to high quality standards and provide access to state-of-the-art technology by
centralized acquisition and clearly defined user agreements and operator regulations.
Function and goals of the Interdisciplinary Centre for Clinical Research
• Strengthening Research
• Funding Excellence
• Networking by Infrastructure
• Convincing by Quality
The Interdisciplinary Center for Clinical Research (IZKF) is the development and strategy
program of the RWTH Aachen faculty of medicine, with the task to strengthen translational
medical research that stems from its basic research and clinic: By supporting top-quality
projects, it strives to greatly improve the chances for solving major and highly challenging
problems in biomedicine and life science.
The IZKF functions as an initiator with vision: funding tools are modified in accordance
with current requirements, thereby exploring different approaches. The IZKF has a major
influence on the research portfolio of the faculty of medicine. By specifically defining the
research focus areas, the IZKF concentrates expertise and provides the basis for the
acquisition of thirdparty-funded collaborations. It represents well established structures that
are in an excellent position to face the current and future challenges of clinical research.
18 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 19Using Institutes and Clinics p. 22
Core Laboratory p. 26
Genomics Facility p. 29
Immunohistochemistry Facility
Confocal Microscopy Facility
p. 32
p. 34
CORE FACILITIES
Brain Imaging Facility p. 37
Two-Photon Imaging Facility p. 43
Transgenic Service p. 48
Proteomics Facility p. 52
Flow Cytometry Facility p. 56
20 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 21Core Facilities | Using Institutes and Clinics Core Facilities | Using Institutes and Clinics
Using Institutes and Clinics
Clinical Departments Core Facilities Department of Prosthodontics and Dental Materials Immunohistochemistry Facility, Confocal Microscopy Facility, Two-Photon
Imaging Facility, Flow Cytometry Facility
Clinic of Accident and Reconstructive Surgery Immunohistochemistry Facility, Flow Cytometry Facility
Teaching and Research Area Dental Materials and Biomaterials Research
Department of Anaesthesiology Immunohistochemistry Facility, Two-Photon Imaging Facility
Department of Psychiatry Brain Imaging Facility
Clinic for Cardiology, Pneumology, Angiology and Internal Intensive Medicine (Med I) Genomics Facility, Immunohistochemistry Facility, Confocal Microscopy Facility,
Two-Photon Imaging Facility, Proteomics Facility, Flow Cytometry Facility Teaching and Research Area Experimental Neuropsychiatry
Department of Cardiac and Thorax Surgery Teaching and Research Area Psychopathology
Teaching and Research Area Child Cardiac Surgery Teaching and Research Area Experimental Behavioral Psychobiology
Department of Child and Adolescent Psychiatry Brain Imaging Facility Teaching and Research Area Structural Functional Brain Mapping
Teaching and Research Area Clinical Child and Adolescent Neuropsychology Teaching and Research Area Structure of Cortical Functional Units
Department of Dental Preservation Brain Imaging Facility Teaching and Research Area Functionality of Cortical Circuits
Teaching and Research Area Oral Microbiology and Immunology Teaching and Research Area Neuropsychological Gender Studies
Department of Dermatology Genomics Facility, Immunohistochemistry Facility, Transgenic Service Department of Oral Maxillofacial and Plastic Facial Surgery Immunohistochemistry Facility
Department of Diagn. and Interv. Radiology Immunohistochemistry Facility, Brain Imaging Facility Department of Radiotherapy
Department of Diagn. and Interv. Neuroradiology Brain Imaging Facility Department of Surgery
Department of General, Visceral and Transplant Surgery Immunohistochemistry Facility, Transgenic Service, Proteomics Facility Department of Urology
Department of Gynaecology and Obstetrics Genomics Facility, Confocal Microscopy Facility, Two-Photon Imaging Facility, Department of Vascular Surgery Immunohistochemistry Facility
Flow Cytometry Facility Internal Medicine II Genomics Facility, Immunohistochemistry Facility, Confocal Microscopy Facility,
Teaching and Research Area Prenatal Medicine Two-Photon Imaging Facility, Transgenic Service, Flow Cytometry Facility
Department of Gynaecological Endocrinology and Reproductive Medicine Internal Medicine III Genomics Facility, Immunohistochemistry Facility, Transgenic Service,
Proteomics Facility, Flow Cytometry Facility
Department of Neurosurgery Immunohistochemistry Facility, Brain Imaging Facility, Two-Photon Imaging
Facility Internal Medicine IV Genomics Facility, Immunohistochemistry Facility, Confocal Microscopy Facility,
Proteomics Facility, Flow Cytometry Facility
Department of Neurology Confocal Microscopy Facility, Brain Imaging Facility, Transgenic Service,
Proteomics Facility Internal Medicine V
Teaching and Research Area Clinical Cognition Internal Medicine VI
Teaching and Research Area Neuropsychology Surgical Intensive Care Confocal Microscopy Facility
Department of Nuclear Medicine Immunohistochemistry Facility, Brain Imaging Facility Institutes
Department of Ophthalmology Genomics Facility, Confocal Microscopy Facility, Two-Photon Imaging Facility Institute of Aerospace Medicine
Teaching and Research Area Experimental Ophthalmology Institute of Applied Microbiology
Department of Orthodontics Flow Cytometry Facility Institute of Anatomy and Cell Biology Immunohistochemistry Facility, Two-Photon Imaging Facility, Transgenic Service
Department of Orthopaedics Immunohistochemistry Facility Institute of Biochemistry and Molecular Biology Genomics Facility, Confocal Microscopy Facility, Proteomics Facility, Flow
Cytometry Facility
Department of Trauma Surgery
Institute of Biochemistry and Molecular Cell Biology Confocal Microscopy Facility, Proteomics Facility
Department of Otorhinolaryngology, Plastic Head, Neck Surgery
Institute for Biochemistry and Molecular Immunology Genomics Facility, Immunohistochemistry Facility, Transgenic Service,
Department of Paediatric Medicine Genomics Facility, Immunohistochemistry Facility, Flow Cytometry Facility
Proteomics Facility
Teaching and Research Area Neonatological Intensive Care
Helmholtz-Institute for Biomedical Engineering Immunohistochemistry Facility, Two-Photon Imaging Facility, Proteomics Facility
Department of Paediatric Cardiology (Applied Medical Engineering)
Department of Palliative Medicine Teaching and Research Area Rehabilitation and Prevention Engineering
Department of Phoniatrics, Pedaudiology and Communication Disorders Brain Imaging Facility, Teaching and Research Area Cardiovascular Engineering
Department of Plastic, Hand and Burns Surgery Immunohistochemistry Facility, Two-Photon Imaging Facility, Proteomics Facility, Teaching and Research Area Tissue Engineering and Textile Implants
22 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 23Core Facilities | Using Institutes and Clinics Core Facilities | Using Institutes and Clinics
Helmholtz-Institute for Biomedical Engineering Genomics Facility, Confocal Microscopy Facility, Two-Photon Imaging Facility, External User
(Experimental Molecular Imaging) Flow Cytometry Facility
DWI – Leibniz Institute for Interactive Materials Immunohistochemistry Facility
Helmholtz-Institute for Biomedical Engineering (Cell Biology) Genomics Facility, Two-Photon Imaging Facility, Transgenic Service, Flow
AICES - Aachen Institute for Advanced Study in Computational Engineering Science
Cytometry Facility
Department of Ceramics and Refractory Proteomics Facility
Teaching and Research Area Stem Cell Biology Genomics Facility, Confocal Microscopy Facility, Two-Photon Imaging Facility
Materials, Faculty 5, RWTH Aachen University
Helmholtz-Institute for Biomedical Engineering (Biointerface) Immunohistochemistry Facility, Confocal Microscopy Facility, Two-Photon
Biology I, Faculty 1, RWTH Aachen University Genomics Facility
Imaging Facility, Transgenic Service
Biology II, Faculty 1, RWTH Aachen University Genomics Facility, Proteomics Facility
Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Genomics Facility, Immunohistochemistry Facility
Chemistry Biology III, Faculty 1, RWTH Aachen University Proteomics Facility
Institute for Computational Genomics Biology V, Faculty 1, RWTH Aachen University Genomics Facility, Immunohistochemistry Facility, Proteomics Facility
Institute for Geriatric Medicine Immunohistochemistry Facility Fraunhofer Institute for Molecular Biology and Applied Ecology Proteomics Facility, Flow Cytometry Facility
Institute of History, Theory and Ethics in Medicine
Institute of Human Genetics Genomics Facility, Immunohistochemistry Facility, Two-Photon Imaging Facility,
Flow Cytometry Facility
Institute for Hygiene and Environmental Medicine
Institute of Immunology Flow Cytometry Facility
Institute of Laboratory Animal Science Immunohistochemistry Facility
Institute of Medical Psychology und Medical Sociology
Institute of Medical Informatics
Institute of Medical Statistics
Institute of Medical Microbiology Genomics Facility, Immunohistochemistry Facility, Transgenic Service, Flow
Cytometry Facility
Teaching and Research Area Virology
Institute of Molecular and Cellular Anatomy Transgenic Service, Flow Cytometry Facility
Institute for Molecular Cardiovascular Research (IMCAR) Genomics Facility, Immunohistochemistry Facility, Two-Photon Imaging Facility,
Transgenic Service, Flow Cytometry Facility
Institute of Molecular Medicine Genomics Facility, Immunohistochemistry Facility, Transgenic Service, Flow
Cytometry Facility
Institute of Neuroanatomy Genomics Facility, Flow Cytometry Facility
Institute of Neuropathology Transgenic Service
Institute for Occupational and Social Medicine Proteomics Facility
Institute of Pharmacology and Toxicology Genomics Facility, Immunohistochemistry Facility, Transgenic Service,
Proteomics Facility
Teaching and Research Area Pharmacology
Teaching and Research Area Pharmacology of Inflammation
Institute of Pathology Genomics Facility, Immunohistochemistry Facility, Two-Photon Imaging Facility,
Transgenic Service, Proteomics Facility, Flow Cytometry Facility
Teaching and Research Area Tumour Pathology
Institute of Physiology Genomics Facility, Immunohistochemistry Facility
Institute of Medical Psychology and Medical Sociology
24 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 25Core Facilities | Core Laboratory Core Facilities | Core Laboratory
Core Laboratory
Equipment and contact persons
All equipment and laboratories can be used upon request and after having consulted the
IZKF administration office and the responsible contact person.
Head of the Core Laboratory: Materials: € 23,330
De Bruyne, K.
Investments/Equipment: € 9,609 Microscopes Wüffel, J. / Ensslen S.
Safety delegate / assistant project supervisor for genetic engineering security:
Preisinger, C. Western Blot Documentation System LAS Preisinger, C. / Gostek, S.
3000
Project supervisor for genetic engineering security:
Preisinger, C. Agilent Bioanalyser 2100 Hübner, J. / Rudzinski, A.
7300 Real Time PCR Taq Man Hübner, J. / Rudzinski, A.
Services
In the Core Laboratory various equipment is provided, that is not correlated to any Core Fluorostar Optima Gostek, S. / Johnen, S.
Facility. Like the Core Facilities, the equipment is not only available for IZKF project heads
or project personnel, but for all the members of the Faculty of Medicine. The equipment Nanodrop Hübner, J. / Rudzinski, A.
also can be used upon request by RWTH scientists. The Core Laboratory provides
infrastructure, lab space and know-how. The personnel of the Core Laboratory provide Photometer Tappe, M. / Wüffel, J.
services for research, assistance to use the equipment and advises in all scientific issues.
The concept aims at handling organizational and technical-methodical challenges and HPLC/FPLC Preisinger, C. / Gostek, S.
assisting the project personnel. Furthermore the centralization of equipment and expertise
aims at conserving resources. Synergetic effects emerge from the collaboration of MALDI-TOF System Preisinger, C. / Gostek, S.
researchers and Core Facilities and these effects are useful for the two-way technical-
methodical assistance that keeps research and service at the highest level. Geldoc – DNA and Protein Ensslen, S. / Gan, L.
Autoclaves Tappe, M. / Wüffel, J.
Users of the Core Laboratory
In 2020 the Core Laboratory was used by 308 employees of 32 institutes and clinics. MilliQ Equipment Wüffel, J.
Med III Cell Culture Laboratory Gostek, S. / Preisinger, C.
Med II
Pathology
Med I Constant Temperature Laboratory Hübner, J.
ZWBF
IZKF
Immunology
Ophtalmology
Other
IBMT-Cellbiology
Paediatrics
IMCAR
AME
LFG ZMG
Plastic Surgery
Humangenetics
Occupational Medicine
Orthopaedics
Orthodontics
Dermatology
General Surgery
Biochemistry
Med. Microbiology
Helmholtz Stemcell
Pharmacology & Toxicology
Neuropathology
Neurology
Mol. Pathobiochemistry
Med IV
Vascular Surgery
Radiology
Neuroradiology
0 5 10 15 20 25 30 35 40
26 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 27Core Facilities | Core Laboratory Core Facilities | Genomics Facility
Usage of the equipment
Genomics Facility
Following overview shows a selection of the equipment of the Core Laboratory and their
usage in 2020:
Equipment Using Clinics/Institutes Total appointments
Head of the facility Staff:
Kurth, I. (Commissary) Abdallah, A. (Bioinformatics)
Nanodrop 15 14.038 samples Gan, L. (Bioinformatics)
Head of Bioinformatics Hübner, J. (Technician)
Agilent Bioanalyzer 3 96 Abdallah, A. Rudzinski, A. (Technician)
Taq Man 6 216 Advisory Board
Costa, I. (Institute of Computational Biology) Materials/Travel expenses 2020: € 194,493
Light Cycler 1 99 Eggermann, T. (Institute of Human Genetics)
Zenke, M. (IBMT – Cell Biology) Investments/Equipment 2020: € 152,896
Autoclaves 11 493 Kramann, R. (Institute for Experimental Internal Medicine and Systems Biology)
Revenues 2020: € 286,328
LAS3000 (Western Blot) 8 207
Skills/Services/Training courses/Consulting service
Cell Culture Laboratory 4 448 The Genomics Facility provides wet lab and bioinformatics expertise to coordinate
competencies and produce high-quality support. In the following, we list the essential
Microscopes 20 373 services and fields of support:
Spectramax 9 302 A) Experimental design: We discuss the factors with our users, which could affect
the experimental design and provide individual support and assistance. To ensure high-
quality experiments, we also go through numerous steps with researchers and assess the
advantages and disadvantages of concrete applications/ methods.
B) Sample preparation: We use starting material from cell culture, tissue, or blood to
extract total RNA, mRNA, miRNA, DNA, etc.. The sample preparation service enables
scientists to implement the newest NGS applications and array technologies in their
projects. For DNA fragmentation we use Covaris Technology. Quality control, quantification
including analyses of DNA, RNA, and protein is done using the 2100 Bioanalyzer/Tape
Station 4200 microfluidics-based platforms (Agilent), quantification using the NanoDrop
technology and Quantus (Promega). During the amplification/labeling procedures, we
monitor all steps by various analyses and controls. Finally, we offer DNA/RNA library
preparation for NGS at various scales of input material.
C) Data generation: We generate genomics data based on two technologies: (1) Array
Hybridization Affymetrix microarray hybridization, washing steps, antibody-staining, and
scanning the arrays according to SOPs. (2) NGS-Sequencing using the NextSeq500
Sequencing System from Illumina.
D) Data analysis: We provide routine data analysis for established NGS applications and
microarrays as optional direct support. Our primary analyses‘ scope depends on the nature
of the application itself and the degree of automation. We also offer tailored solutions on a
collaborative basis to project specific issues. Advanced analysis can be, e.g., new cutting-
edge applications and technologies, advanced data analysis methods, data visualization,
and method optimization. Additionally, we offer project consulting and support by assisting
researchers through all research phases. We complete these services by managing raw
data, results, tools, and procedures, which are especially essential for NGS data analysis,
enabling access to data and analysis results through a web interface. Finally, we consult
researchers in selecting and using established bioinformatics tools.
E) Training and Seminars: We organize trainings for our user and co-organize the NGS
user club.
28 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 29Core Facilities | Genomics Facility Core Facilities | Genomics Facility
Users
115 users of 24 clinics/institutes of the medical faculty of the RWTH Aachen used the
Genomics Facility.
The Genomics Facilty had external user from the Institute of Biology II, III and V of Faculty 1
of the RWTH Aachen, the University Hospital Bonn, the UMC Maastricht and the Antbodies
online GmbH.
Collaborations
The Genomics Facility had project collaborations with the Medical clinic and polyclinic I -
Laboratory for Innate Cellular Immunology and the Polyclinic for Orthodontics of the
University of Bonn and the Biology II and V of the RWTH Aachen.
Publications
Krenkel O, Hundertmark J, Abdallah AT, Kohlhepp M, Puengel T, Roth T, Branco DPP,
Mossanen JC, Luedde T, Trautwein C, Costa IG, Tacke F (2020) Myeloid cells in liver and
bone marrow acquire a functionally distinct inflammatory phenotype during obesity-related
Status and Development steatohepatitis. Gut. 69(3):551-563. doi: 10.1136/gutjnl-2019-318382. [IF 17.943]
In 2020, the Genomics Facility was able to further develop methodologically and in terms
of equipment. We extended our wet lab infrastructure by the CellenOne F1.4 machine, Buhl EM, Djudjaj S, Klinkhammer BM, Ermert K, Puelles VG, Lindenmeyer MT, Cohen CD,
a microplate-based single-cell isolation system with 4-fluorescent excitation channels, He C, Borkham-Kamphorst E, Weiskirchen R, Denecke B, Trairatphisan P, Saez-Rodriguez
system-tailored single-cell application solutions, and compatible with other single-cell J, Huber TB, Olson LE, Floege J, Boor P (2020) Dysregulated mesenchymal PDGFR-β drives
systems (iCell8) that are established at our Faculty. The synergy between these modern kidney fibrosis. EMBO Mol Med. 2(3):e11021. doi: 10.15252/emmm.201911021. [IF 10.293]
systems is expected to greatly improve the support scope of cutting-edge genomics
applications in the Faculty. This will contribute significantly to the performance of high- Baumeister J, Chatain N, Hubrich A, Maié T, Costa IG, Denecke B, Han L, Küstermann C,
quality research including single-cell sequencing. Sontag S, Seré K, Strathmann K, Zenke M, Schuppert A, Brümmendorf TH, Kranc KR,
Koschmieder S, Gezer D (2020) Hypoxia-inducible factor 1 (HIF-1) is a new therapeutic
Importantly, we are currently involved in many active collaborations in and outside of target in JAK2V617F-positive myeloproliferative neoplasms. Leukemia. 34(4):1062-1074.
RWTH-Aachen. For instance, we are supporting projects involving data analysis of single- doi: 10.1038/s41375-019-0629-z. [IF 8.665]
cell immune profiling and single-cell RNA-Seq with cell hash-tagging (HTO) from 10x, which
are also applications at the cutting edge of the single-cell technology. Rose M, Maurer A, Wirtz J, Bleilevens A, Waldmann T, Wenz M, Eyll M, Geelvink M,
Gereitzig M, Rüchel N, Denecke B, Eltze E, Herrmann E, Toma M, Horst D, Grimm T,
We are also supporting a couple of additional NGS applications. For instance, the support Denzinger S, Ecke T, Vögeli TA, Knuechel R, Maurer J, Gaisa NT (2020) EGFR activity
of 3’ mRNA-Seq started this year in productive mode and is anticipated to become a addiction facilitates anti-ERBB based combination treatment of squamous bladder cancer.
constituent part of our NGS service repertoire. Due to the high sample multiplexing potential Oncogene. 39(44):6856-6870. doi: 10.1038/s41388-020-01465-y. [IF 7.791]
of this application and the lower needed number of reads per sample, it has already
been an option for several users. It is expected to ultimately replacing the corresponding Zechendorf E, O‘Riordan CE, Stiehler L, Wischmeyer N, Chiazza F, Collotta D, Denecke
microarrays. Furthermore, we are now establishing CUT&RUN, a new NGS application, B, Ernst S, Müller-Newen G, Coldewey SM, Wissuwa B, Collino M, Simon TP, Schuerholz
regarded as a cheap and more qualitative alternative to ChIP-Seq. T, Stoppe C, Marx G, Thiemermann C, Martin L. (2020) Ribonuclease 1 attenuates septic
cardiomyopathy and cardiac apoptosis in a murine model of polymicrobial sepsis. JCI
Regarding the quality of sequencing services, we have introduced the shallow sequencing Insight. 5(8):e131571. doi: 10.1172/jci.insight.131571. [IF 6.205]
approach in a couple of use cases to optimize our single-cell sequencing workflow.
Furthermore, we are now using sophisticated saturation analyses to compute needed reads Müller AK, Markert N, Leser K, Kämpfer D, Schwiy S, Riegraf C, Buchinger S, Gan L,
for resequencing experiments accurately. The approach is saving project costs and time in Abdallah AT, Denecke B, Segnere H, Brinkmann M, Crawford SE, Hollert H. (2020)
the long run. We are planning to extend the usage of this approach to most of our single- Bioavailability and impacts of estrogenic compounds from suspended sediment on rainbow
cell projects. trout (Oncorhynchus mykiss). Aquat Toxicol. [In Press] [IF 4.344]
Consulting for NGS projects this year was mostly offered via virtual meetings. Often, we Vaughan D, Ritvos O, Mitchell R, Kretz O, Lalowski M, Amthor H, Chambers D, Matsakas
have involved two scientists and sometimes one technical expert in these meetings. This A, Pasternack A, Collins-Hooper H, Ballesteros R, Huber TB, Denecke B, Widera D,
allowed for an improved level of experimental design assistance. We could also speed up Mukherjee A, Patel K. Inhibition of Activin/Myostatin signalling induces skeletal muscle
meetings appointment via MS TEAMS and have used the possibilities of virtual tools to hypertrophy but impairs mouse testicular development. (2020) Eur J Transl Myol.
present our analyses and to better understand customer‘s study design. 30(1):8737. doi: 10.4081/ejtm.2019.8737. [IF 1.4]
30 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 31Core Facilities | Immunohistochemistry Facility Core Facilities | Immunohistochemistry Facility
Immunohistochemistry Facility
Status and Development
Overall, services and the number of users have decreased in 2020 compared to 2019. We
suspect that this is due to the Corona pandemic.
In 2020 (2019) 160 (213) researchers from 35 (32) different clinics/institutes of the medical
Head of the facility Staff: faculty at the RWTH Aachen and 14 (18) researchers from 4 (5) extern institutes used the
Ensslen, S. Ensslen, S. Immunhistochemistry Facility. The main users are from the Department of Internal Medicine
Tappe, M. II and III.
Advisory Board Wüffel, J.
Ostendorf, T. (Medical Clinic II - Clinic for Renal and Hypertensive Disorders, A total of 18681 (20791) tissue samples were dehydrated and embedded. 14951 (16837)
Rheumatological and Immunological Diseases) Materials/Travel expenses 2020: € 40,470 slices were produced and 3463 (4322) slides were stained. 551 (884) immunhistological
Boor, P. (Pathology Institute) staining procedures were performed.
Investments/Equipment 2020: € 6,969
Skills/Services/Training courses/Consulting service Revenues 2020: € 33,522
Histological studies are used in many fields such as research, diagnosis, and education
to visualize the molecular and structural components of tissues and cells and to detect
structural integrity or alterations of the integrity in diseased tissue.
Besides classical histological staining, immunhistological staining is widely used in diagnosis
and basic research to characterize cells and tissues.
Our Immunohistochemistry Facility offers competent guidance and support in planning and
lead-through of histological and immunohistological experiments.
The following service can be offered:
• pre-experimental consulting
• support to excision and preparation of samples and tissues (choice of proper fixatives)
• dehydration and embedding of fixed tissues
• preparation of tissue slices (paraffin and cryo)
• histological staining (H&E, EvG, Giemsa, PAS etc.)
• direct and indirect immunohistological staining
• testing of antibodies (according to prior agreement)
• microscopy and documentation (according to prior agreement)
The Team of the Immunohistochemistry Facility
32 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 33Core Facilities | Confocal Microscopy Facility Core Facilities | Confocal Microscopy Facility
Confocal Microscopy Facility
• 3-dimensional confocal images
- Preparation of layered image stacks („z-stacks“)
- Reconstruction of 3-dimensional objects
• Live-cell imaging under cell culture conditions (control of temperature and CO2).
Head of the facility Staff: Advanced microscopy
Müller-Newen, G. (Institute of Biochemistry and Molecular Biology) Ernst, S. • Pre-experimental consulting and hands-on training
• FRAP (fluorescence recovery after photobleaching) and FLIP (fluorescence loss in
Advisory Board photobleaching) to determine the mobility of fluorescently labeled molecules in living
Lüscher, B. (Institute of Biochemistry and Molecular Biology) Materials/Travel expenses 2020: € 12,770 cells
• FRET (Förster resonance energy transfer) to detect interaction of fluorescent
Investments/Equipment 2020: € 9,290 molecules
Skills/Services/Training courses/Consulting service • Use of photoconvertible and photoactivatable fluorescent proteins to determine
Confocal microscopy generates detailed images from fluorescently labelled samples. Out- Revenues 2020 € 17,433 protein dynamics in living cells
of-focus light that blurs the image in conventional microscopy cannot pass the confocal
pinhole and is therefore eliminated. A 3-dimensional representation of a sample can
be generated from serial optical sections. Confocal microscopy is performed on tissue Status and Development
sections, fixed cultured cells or even with living cells containing an appropriate fluorescence With the beginning of 2021 the CMF has been part of the IZKF Core Facilities for more than
label. Laser light is used for efficient excitation of fluorescently labelled samples. The nine years. In 2020, the CMF has been used by 17 institutes and clinics of the Medical
various lasers of a confocal microscope can also be used as a precision tool to modulate Faculty. In most cases, more than one individual of the respective department was involved
fluorescence in living cells with subcellular resolution. Based on this property, advanced as a user. Thus, the number of users exceeds 40. At least 11 papers have been published
fluorescence techniques have emerged that can give access to intracellular dynamics and in 2020 with support of the CMF (refs. 1-11).
interactions of fluorescently labelled biomolecules.
In context of the Art. 91b GG application by Dr. Rafael Kramann and colleagues a new
The Confocal Microscopy Facility (CMF) operates two confocal microscopes: Nikon A1-Ti2-N-STORM confocal & super-resolution microscope has been installed within
the facility in May 2018. This microscope is used by the CMF (20%) and by the other
• Zeiss LSM 710 (inverted) applicants of the proposal (80%). After some recurrent technical problems were solved
- Laser lines 405, 458, 488, 514, 561 and 633 nm in 2019 by substitution of hardware components, the demanding technique of super-
- Spectral detection unit resolution microscopy through single molecule localization will be further established in this
- Motorized stage funding period (2020-2023).
- Incubator for live-cell imaging
Moreover, the installation of a new state-of-the art Zeiss LSM980 with AiryScan2 in addition
• Nikon A1-Ti2-N-STORM (inverted) to the 11-year-old Zeiss LSM710 is a major goal for 2021. A proposal for the Zeiss LSM980
- Laser lines 405, 445, 488, 514, 561 and 647 nm has been submitted to the Dean of the Medical Faculty and will be approved by the
- Spectral detection unit Planning and Allocation Committee of the Exzellenzinitiative at RWTH Aachen University.
- Motorized stage
- N-STORM unit for single molecule localization super-resolution microscopy (SMLM)
Users within the faculty
• All microscopes are located in an air-conditioned darkroom 40 users from 17 different clinics/institutes of the medical faculty of the RWTH Aachen used
the Confocal Microscopy Facility.
The following services are offered by the CMF:
Confocal imaging Publications
• Individual consulting on the application of confocal microscopy in research projects. Dohmen M, Krieg S, Agalaridis G, Zhu X, Shehata SN, Pfeiffenberger E, Amelang J,
• Advice on sample preparation. Butepage M, Buerova E, Pfaff CM, Chanda D, Geley S, Preisinger C, Sakamoto K, Luscher
• Individual hands-on training on the confocal microscope adapted to the depth of use. B, Neumann D, Vervoorts J (2020) AMPK-dependent activation of the Cyclin Y/CDK16
• 2-dimensional confocal images complex controls autophagy. Nat Commun 11, 1032 [IF 12.1]
- Multi-channel images with up to 4 fluorophores
- Exact overlay of fluorescence with differential interference contrast (DIC) images Hollmann J, Brecht J, Goetzke R, Franzen J, Selich A, Schmidt M, Eipel M, Ostrowska
- Fluorescence intensity profiles A, Hapala J, Fernandez-Rebollo E, Muller-Newen G, Rothe M, Eggermann T, Zenke
- Colocalization analysis M, Wagner W (2020) Genetic barcoding reveals clonal dominance in iPSC-derived
mesenchymal stromal cells. Stem Cell Res Ther 11, 105 [IF 5.1]
34 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 35Core Facilities | Confocal Microscopy Facility Core Facilities | Brain Imaging Facility
Brain Imaging Facility
Juhlen R, Martinelli V, Vinci C, Breckpot J, Fahrenkrog B (2020) Centrosome and ciliary
abnormalities in fetal akinesia deformation sequence human fibroblasts. Sci Rep 10, 19301
[IF 4.0]
Lin YY, Schuphan J, Dickmeis C, Buhl EM, Commandeur U, Fischer H (2020) Attachment
of Ultralow Amount of Engineered Plant Viral Nanoparticles to Mesenchymal Stem Cells Head of the facility Staff:
Enhances Osteogenesis and Mineralization. Adv Healthc Mater 9, e2001245 [IF 7.4] Rodriguez-Raecke, R. Rodriguez-Raecke, R.
Poznansky, O.
Moreno-Andres D, Yokoyama H, Scheufen A, Holzer G, Lue H, Schellhaus AK, Weberruss Advisory Board Schüppen, A.
M, Takagi M, Antonin W (2020) VPS72/YL1-Mediated H2A.Z Deposition Is Required for Binkofski, F. C. (Department of Neurology) Ritter, C.
Nuclear Reassembly after Mitosis. Cells 9 [IF 4.4] Mathiak, K. (Department of Psychiatry) Sijben, R.
Habel, U. (Department of Psychiatry) Eder, G.
Qiao Z, Wang W, Luo P, Hofman M, Horst K, Muller-Newen G, Greven J, Hildebrand F Wiesmann, M. (Department of Diagnostic and Interventional Neuroradiology) Richter, F.
(2020) The Impact of Plasma-Derived Microvesicles From a Femoral Fracture Animal Model Nitzpon, L.
on Osteoblast Function. Shock 53, 78-87 [IF 3.0] Fuchs, A.
Shi Y, Luo P, Wang W, Horst K, Blasius F, Relja B, Xu D, Hildebrand F, Greven J (2020) M1
But Not M0 Extracellular Vesicles Induce Polarization of RAW264.7 Macrophages Via the Materials/Travel expenses 2020: € 8,148
TLR4-NFkappaB Pathway In Vitro. Inflammation 43, 1611-1619 [IF 3.2]
Investments/Equipment 2020: € 74,105
Woltje M, Brunler R, Bobel M, Ernst S, Neuss S, Aibibu D, Cherif C (2020) Functionalization
of Silk Fibers by PDGF and Bioceramics for Bone Tissue Regeneration. Coatings 10 [IF 2.4] Revenues 2020: € 30,753
Yamoah A, Tripathi P, Sechi A, Kohler C, Guo H, Chandrasekar A, Nolte KW, Wruck
CJ, Katona I, Anink J, Troost D, Aronica E, Steinbusch H, Weis J, Goswami A (2020)
Aggregates of RNA Binding Proteins and ER Chaperones Linked to Exosomes in
Granulovacuolar Degeneration of the Alzheimer‘s Disease Brain. J Alzheimers Dis 75, 139-
156 [IF 3.9]
Zaja R, Aydin G, Lippok BE, Feederle R, Luscher B, Feijs, KLH (2020) Comparative analysis
of MACROD1, MACROD2 and TARG1 expression, localisation and interactome. Sci Rep
10, 8286 [IF 4.0]
Zechendorf E, O‘Riordan, CE, Stiehler L, Wischmeyer N, Chiazza F, Collotta D, Denecke The Team of the Brain Imaging Facility
B, Ernst S, Muller-Newen G, Coldewey SM, Wissuwa B, Collino M, Simon TP, Schuerholz
T, Stoppe C, Marx G, Thiemermann C, Martin L (2020) Ribonuclease 1 attenuates septic
cardiomyopathy and cardiac apoptosis in a murine model of polymicrobial sepsis. JCI
Insight 5 [IF 6.2] Skills/Services/Training courses/Consulting service
The Brain Imaging Facility (BIF) is a central support unit for all neuroimaging research at
the Faculty of Medicine of the RWTH Aachen University, providing technical and scientific
support at all stages of research projects. The BIF offers these services to approximately 18
Applied and actual third-party funding different research groups from 10 clinical departments. The BIF works in tight collaboration
with both the departments of Neurology and Psychiatry, who provide two research-
S. Uhlig RWTH Life Sciences Core Facilities Exzellenzinitiative applied € 1,525,280 dedicated Siemens 3 Tesla MRI PRISMA scanners, equipped with 20-, 32-, and 64-channel
G. Müller-Newen RWTH Aachen head coils as well as head coils for phosphorus and sodium imaging. The BIF provides
C. Preisinger state-of-the-art MR compatible equipment such as visual (projectors, LC displays, goggle
P. Walter systems) and auditory (both with and without active noise cancellation) stimulation devices,
eye-trackers, response devices, physiological measurement hardware as well as hardware
and software for real-time fMRI-based applications such as neurofeedback. In addition
to assistance at the stage of project preparation, the BIF also supports data collection,
storage, and analysis up to obtaining final results and publications.
36 IZKF Aachen Progress Report 2020 IZKF Aachen Progress Report 2020 37You can also read
