DESI ION SOURCES Desorption Electrospray Ionization
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DESI ION SOURCES
Desorption Electrospray Ionization
Enabling innovative protocols
from pharmaceuticals to forensics
from food to environmental.
Get from sample to results faster with DESI.
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or text “DESI” to 22828 to get started.
Contents Company Overview
Direct Sample Analysis.................................2
About Prosolia
Examples of Impact.....................................3 Prosolia, Inc. is a scientific instrument company
engaged in the development, marketing and
DESI Mass Spectrometry.............................4
distribution of innovative sample introduction
DESI Applications..........................................5 technologies for mass spectrometry systems that
DESI Ion Source Products..........................6 address the rapidly evolving needs of diverse
customers in the Life Science, Pharmaceutical and
DESI Ion Source Software...........................7
Biotechnology, Clinical, Materials, Food and
Accessories & Consumables......................8 Agriculture end-markets. Our portfolio of scientific
FireflyTM Data Conversion Software........9 analytical tools includes innovative sample
introduction systems and intuitive software, all of
which are part of workflows that reduce complexity
and accelerate results. These tools apply mass
spectrometry technologies to enable scientists to
conduct quantitative and qualitative analysis,
including mass spectrometry imaging across a
wide range of applications.
Prosolia’s products enable scientists to:
• Explore where drugs and their metabolites
distribute using mass spectrometry imaging.
• Quantify drugs in biological fluids and
tissue sections.
• Discover biomarkers of cancer and other
diseases in biopsy tissue.
• Identify counterfeit medicines, contaminants in
drug and materials manufacturing, pesticides
in agricultural products and chemical agents
in the field.
1
Direct Sample Analysis
Increase the speed of analytical experimentation with the
Desorption Electrospray Ionization (DESI) ion source coupled to your
mass spectrometer. DESI combines real-time micro-scale solvent
extraction in fast moving droplets with electrospray ionization (ESI)
to achieve high throughput sample introduction for mass spectrometry
without sample preparation.
Addressing the sample prep bottleneck…
A bottleneck in life sciences research and chemical analysis is the
time required for sample preparation. Sample preparation is a tedious
process that often involves multiple steps, delaying the time to a
result, decreasing productivity and increasing the probability for error.
Prosolia’s DESI technology eliminates this bottleneck by enabling direct
analysis of samples thereby reducing the requirement for sample
preparation. These capabilities translate into lower laboratory
operational costs, streamlined workflows and generate results faster
than traditional approaches.
2
Prosolia’s products help solve
customer’s problems in a
number of markets.
We outsource
autoradiography (AR). Examples of impact include:
A single AR contract is roughly $80K
and takes 3 months plus $20-30K to
• Developing safer drugs
In pharma, scientists are using Prosolia technology to
synthesize the radio label. DESI has determine the distribution of drugs in animal tissues
eliminated the need for these studies during drug discovery research.
in these early R&D phases. It’s cost
• Detecting counterfeit medicines
effective. Elimination of one AR
In academia, scientists are using Prosolia technology to
study has justified the expense. rapidly and directly identify counterfeit antimalarial drugs.
We can see both the molecule and
metabolites in our studies with DESI. • Better drug manufacturing
In pharma, analytical scientists are using Prosolia
Easy to use. Training was excellent. technology to screen tablets for degradant products
My VP sees value in the source. or monitor reactions near the vessel.
Principal Scientist • Accelerating results in the study of disease
Biotech Customer In academia, scientists are using Prosolia technology to
identify biomarkers of cancers in resected tissue specimens
to better delineate tumor margins during surgery, which will
result in improved patient outcomes.
• Monitoring quality of consumer electronics
Consumer electronics manufacturers are using Prosolia
technology to analyze the surfaces of liquid crystal displays
for organic contaminants.
• Formulating new consumer products
Consumer products companies are using Prosolia
technology to develop better laundry detergents and study
lipids in cosmetics and oils.
3
DESI Mass Spectrometry
Principle of Operation
Desorption Electrospray Ionization (DESI) is DESI nozzle
carried out by directing high velocity charged High velocity
droplets produced from a pneumatically-assisted charged droplets
electrospray onto a surface to be analyzed at
atmospheric conditions. Ions of chemical
species present on the surface are produced Mass
Spectrometer
through the interaction of the charged droplets Inlet
and the sample. The resulting mass spectra are
similar to ESI mass spectra.
Capabilities
• Rapid quantitation, 6s per sample • Tunable lateral spatial resolution from 50µm—2mm
• Mass spectrometry imaging without • Analysis of conductive and non-conductive surfaces
matrix under atmospheric conditions
Benefits
• Direct analysis • Analysis of conductive and
• No matrix non-conductive surfaces;
• No heat • No radiolabelled compounds are required for
your drug and metabolite studies;
• Low maintenance
• Low consumable burden • Widest instrument compatibility;
• Samples are held at atmospheric (e.g. Time of Flight, Orbitrap, FTMS, Ion Trap)
pressure not under vacuum;
• Broad vendor compatibility; (i.e. Agilent, Thermo
• Simple sample preparation Scientific, Waters, AB Sciex, Bruker and LECO)
– No matrix is applied to the surface;
• Simultaneous localization of hundreds to
thousands of molecules;
4
DESI Applications
DESI Imaging
Perform mass spectrometry imaging without matrix application to the surface. Why wait to analyze your
samples? Implement MS imaging on your instrument.
Label Free Imaging of Drugs
• Simultaneous localization and identification of
drugs and metabolites in animal tissue sections
• Research and identification of drugs, metabolites
and endogenous markers
• Kinetic studies on drug distribution and degradation
Drug Imaging Lipid Imaging
Lipid Imaging
• Map fatty-acids, glycerolipids, glycerophospholipids,
sphingolipids, triglycerides, sterol-containing
metabolites and other lipid classes in tissue sections
and other surfaces.
In Vivo/In Vitro Applications
DESI 1-D and 2-D ion sources enable rapid quantitation
of drugs in urine, plasma, dried blood spots (DBS) and
microsomal incubations. Bacterial Profiling
Forensics
DESI enables direct read-out of Thin-Layer Chromatography
(TLC) Plates, both normal-phase and reverse-phase, without
any sample treatment, for the analysis of drugs of abuse and
other illicit substances. Manual and 1-D systems can
accommodate standard 76mm x 26mm TLC plates while the
DESI 2-D system can accept samples of a larger footprint,
including two (2) 5cm x 5cm HPTLC plates. TLC/DESI-MS of Cannabinoids
Pharmaceutical Tablets
DESI is uniquely suited for the direct tablet analysis using mass
spectrometry due to its non-destructive nature and imaging
capability while the sample is situated outside the vacuum of the
mass spectrometer. While DESI 2-D systems can perform tablet
imaging studies, Manual and 1-D systems are also suitable for
spot sampling of the tablet surface.
Tablet Imaging
5
DESI Ion Source Products
DESI 2-D Ion Source
• X-Y motorized sample platform for high throughput and
imaging applications
• Sample platform is microtiter footprint and can hold two
or three 76 x 26mm microscope glass slides
• Software controlled with intuitive GUI
• Easy to install and remove allowing for rapid turnaround OS-3201
when switching to another ion source
• Protective source enclosure
DESI 1-D Ion Source
• X-axis motorized sample platform; manual Y-axis
• Sample platform accommodates 76 x 26mm microscope
glass slides including standard Omni Slide substrates
• Software controlled with intuitive GUI
• Easy to install and remove allowing for rapid turnaround
when switching to another ion source
OS-3101
DESI Manual Ion Source
• Manual X and Y sample platform
• Sample platform accommodates 76 x 26mm microscope
glass slides including standard Omni Slide substrates
• Upgradeable to the DESI 1-D system
OS-3001
Available for:
Contact a Prosolia representative to get the latest information on product compatability. Email: sales@prosolia.com
6
DESI Ion Source Software
DESI 2-D Ion Source Motion
Control Software
• Software control of X-Y motion
• Triggers MS to synchronize data acquisition
• Integrated video capture to view sample analysis
location in real time
• Motion control features:
- Point-to-point analysis
- Constant velocity scanning
DESI 2-D Motion Control Software
- Manual control
DESI 1-D Ion Source Motion
Control Software
• Software control of X-motion
• Triggers MS to synchronize data acquisition
• Integrated video capture to view sample analysis
location in real time
• Motion control features:
- Point-to-point analysis
- Constant velocity scanning
- Manual control DESI 1-D Motion Control Software
Software Integration
The DESI ion source is operated using independent motion
control software. Use the standard vendor method editors
and data acquisition systems to set-up sample sequences
and process your data. You simply build a batch sequence
and execute the routine in the vendor software as usual.
Vendor Method Editor Vendor Data System
Xcaliber is a trademark of Thermo Fisher Scientific.
7
Accessories & Consumables
HPTLC plate holder for DESI 2-D platform
• Holds two (2) 5 cm by 5 cm (HP)TLC plates
• Rests firmly on the DESI 2-D sample platform
DESI Emitters
• Precision cleaved fused silica capillary
• No jagged, chipped ends
• Provides stable, reproducible DESI spray
Omni Slide™ Hydrophobic Surfaces
Omni Slide Hydrophobic Arrays for reproducible sample spotting
and improved signal stability in high-throughput and quantitative studies.
Features:
• Hydrophobic coating
• Positive sample location
• 66 or 96 sample arrays
Ion Transfer Tubes
Extended ion transfer tubes allow for the analysis of large sample areas using the
1-D and 2-D DESI ion sources. The bent ion transfer tube allows for samples to pass
freely beneath the tube for analysis and is easily installed/removed from the MS.
Waters AB Sciex Thermo Scientific
Contact your Prosolia representative to determine the appropriate ion transfer tube and assembly for your mass spectrometer.
8
FireFly™ Data Conversion Software
DESI Ion Source users now have the capability to convert their large
data sets into file formats compatible with third party software using
FireFly™ data conversion software. FireFly is compatible with
Thermo Scientific, Bruker, LECO, Waters and AB Sciex data
acquisition systems.
FireFly is a software program that extracts and builds data sets from
data systems data conversion algorithms into file formats compatible
with BioMAP, a third party image visualization and processing
software program. FireFly™ Data Conversion
FireFly was created in order to enable visualization of
multi-dimensional data sets, which Prosolia’s DESI 2-D Ion Source
can routinely generate in combination with mass spectrometers
from select manufacturers.
Firefly™ 2.0 Features
• Compatibility with Thermo Scientific Xcalibur™, Waters Mass Lynx™,
AB Sciex Analyst™, Bruker Compass™ software, and
LECO ChromaToF™ data acquisition software*†
• Improved data conversion efficiency
• Data conversion using Automatic Gain Control (AGC) or equivalent
ion trap scan functions Biomap
• Auto Scaling
BioMap, a common MS image data evaluation
• Optional file conversion without truncating data for BioMAP‡ platform for Unix, Windows and MAC,
was written in IDL™. It provides a common
visualization and storage platform, which
can be used for visualization of data from
any source, provided that an import filter
exists for this format. This platform can be
* Compatibility restrictions apply;
extended by various software packages,
† Xcalibur is a trademark of Thermo Fisher Scientific, Mass Lynx is a trademark of Waters Corp., Analyst is a individually designed for analysis of specific
trademark of AB Sciex, Compass is a trademark of Bruker Daltonics, ChromaToF is a trademark of LECO Corp. data sets. Visualization is based on multiplanar
reconstruction allowing extraction of
‡Not compatible with BioMAP. Requires additional software that reads Analyze 7.5 file formats. arbitrary slices from a 3D-volume. (Not a
Prosolia product.)
Firefly is a trademark of Prosolia, Inc.
9DESI Ion Source Demonstration Contact Prosolia today to learn how to evaluate DESI for your application either in your laboratory or ours. Our Demonstration Laboratory is located in downtown Indianapolis, Indiana just 20 minutes from the Indianapolis International Airport. The laboratory boasts ion trap, ToF and triple quadrupole mass spectrometers. Prosolia, Inc. 351 West 10th Street, Suite 316 Indianapolis, IN 46202 Phone: 1-866-241-0239 Fax: 1-317-275-5799 Email: info@prosolia.com www.prosolia.com Please contact us with any questions or comments.
351 West 10th Street, Suite 316, Indianapolis, IN 46202 Phone: 1-866-241-0239 Fax: 1-317-275-5799
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