Preservative Efficacy of Selected Essential Oils on Strawberry
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Journal of Advances in Nanotechnology and its Applications
Volume 3 Issue 1
Preservative Efficacy of Selected Essential Oils on Strawberry
Deep R. Darji*1, Palak Sapra2, Archana U. Mankad3
Department of Botany, Bioinformatics and Climate Change Impacts Management, School of
Science, Gujarat University, Ahmedabad, Gujarat, India.
*Corresponding Author
E-mail Id:-darjideep5@gmail.com
ABSTRACT
Preservative efficacy of selected natural essential oils was studied on strawberry. Essential
oils employed as phytochemicals researches and practices. Essential oils using in many fields
like pharmaceuticals, pharmacognosy fields and medicinal fields. Cinnamon oil, clove oil and
eucalyptus oil are used in many fields as medicinal properties. Essential oils has also
preservative efficacy. Some essential oils are used as preserve some historical things, plants
parts, animal parts and organs parts. Preservative effects mainly depend on essential oils
chemical constituents. This research provides on preservative efficacy of selected essential oils
on strawberry.
Keywords:-Chemical preservative, natural selected essential oils
INTRODUCTION Cloves (Syzygium aromaticum) are
According to lifetime strawberry have short aromatic flowers buds from an evergreen
life shelf. Because the high of susceptibility tree belonging to the myrtaceae plant
to mycosis (fungal infection) and its agent family [5,6]. They’re considered medicinal
[1]. The employment of artificial antifungal plants in additional spices in china and
chemical compounds has been a priority for eastern countries again bacterial and fungal
increasing the period of time of this fruits diseases [7]. This 8-12 meter plant grows in
[1]. In step with distribution of world, tropical climates such as Indonesia, India,
strawberry could be a widely grown hybrid the West Indies, Brazil, Sri Lanka,
species of the fragaria. For this reason, it’s Tanzania, and Madagascar [8]. Clove buds
necessary to use safe methods to regulate contain 15-20% violet essential oils, it’s
decomposition and maintain the standard of mainly having dominated chemical
strawberry fruit during storage [1]. component eugenol (70-80%) [9-11].
The bark and leaves of varied cinnamon Eucalyptus, it’s an oversized genus of over
species is a very important spice is 660 species of shrub and trees of the
employed everywhere the planet [2]. myrtaceae family [12]. Eucalyptus plant
Cinnamon aromatic plant isn’t only used parts are having bark, leaves, flower, fruits
for cooking but they need used as medicinal during which they’re use in several bio-
properties and it also utilized in traditional activities. Consistent with worldwide, fever
medicine [2]. The genus of cinnamon has tree is that the principle of source of
three main spices used as bark and powder eucalyptus volatile oil. Fever tree essential
[3], it will be found on the market [4] oils also known as volatile aromatic oil
Cinnamon has high medicinal potential and [13]. For this reason, essential oils are
big selection of possible positive effect for extracted from plants for clinically
flesh and source [3]. purposes have become an important topics
in research and industrial application likely
HBRP Publication Page 1-6 2021. All Rights Reserved Page 1Journal of Advances in Nanotechnology and its Applications
Volume 3 Issue 1
their different biological activities of oils, Strawberries were collected from retail fruit
which exercise antimicrobial [14], anti- shop, Ahmedabad.
oxidant [15] and anti-inflammatory [16,17].
Growth of Fungal Strain
The aim of this research was to test in in- Strawberries were collected from retail fruit
vitro condition, preservative efficacy of shop; it should be fresh. Strawberries were
selected natural essential oil on strawberry put in four different sterilized small
for preservative purposes. containers. Strawberry samples should be
kept on top thing is condition. Effect of
MATERIAL AND METHOD environment factor, humid and moist
Collection of Essential Oils affected in two to three days.
Essential oils (cinnamon oil, clove oil and Total four specimen control specimens
eucalyptus oil) were collected from retail (X0), no any kind of treatment on this
oil shop, Ahmedabad. control specimen. For preservative
Collection of Strawberry purposes, strawberries were put deep in all
three oils.
Number of Specimens
specimens
X0 Control strawberry
X1 Strawberry deep in cinnamon oil
X2 Strawberry deep in clove oil
X3 Strawberry deep in eucalyptus oil
X0 X1 X2 X3
Specimens
In Vitro Assay to Check Preservative treatment of oil and deep in oils. Observed
Activity fungus identified as Rhizopus stolonifer.
All the containers which were added for
performing the experiment were sterilized RESULT AND DISCUSSION
by using ethanol and sterilization. All four The result showed significant preservative
specimens were disinfected by surface efficacy of selected essential oils on
sterilization. Strawberries were incubated strawberries. It inhibits bacterial and fungal
inside the sterile containers. Observations infection.
were done under room environment.
Strawberry
These methods are repeated for 4 month. At According to research study on three
the tip of incubation period time, antifungal different essential oils, cinnamon oil, clove
activities are observed. All four specimens oil and eucalyptus oils have beneficial
X0, X1, X2, and X3 under stable light and preservative properties. It inhibited
dark in condition. The infective stage was contamination like bacterial and fungal
accelerated in two to three days on infections. According to selected essential
specimen X0. Because it should be in pack oils, cinnamon, clove and eucalypts oils
in condition and don’t have any kind of have strong preservative effects. (Table-1)
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Volume 3 Issue 1
Table 1:-Inhibitory effects of essential oils.
Specimens Mycelia Inhibition
growth
X0 + Io
X1 * I
X2 * I
X3 * I
(I-Inhibit, Io- Infected), [+] = present, [*] =absent
According to morphological evidences, all identified as Rhizopus stolonifer. After
specimens are stable on control day. X0 week it totally spoiled. X1, X2 and X3
specimen loses their ability and activity started changes after 15 days size and
after two to three days. On this specimen colour changes re seen but it stable and
fungal infection showed on third day. Fungi preserve up to 4 months. (Table-2)
Table 2:-Morphological evidences
Days X0 X1 X2 X3
Io S C Io S C Io S C Io S C
Control day - - - - - - - - - - - -
1-5 days + + + - - - - - - - - -
5-15 days + + + - - - - - - - - -
15-30 days + + + - - + - - - - - -
2 month + + + - + + - + + - + +
3 month + + + - + + - + + - + +
4 month + + + - + + - + + - + +
[+] = present, [-] = absent, [Io] = infected, [S] = size changes, [C] = colour changes.
According to microscopic evidences, X0 X3 specimens haven’t any kind of
specimen has fungal infection and their contamination. (Table-3). Identified
development. Sporangium development evidences and observation are shown in
and mycelia growth are seen. X1, X2 and Table 4 and 5.
Table 3:-Microscopic evidences
Months Specimens Fungal growth (spore and sporangium
development)
1-4 months X0 +
X1 *
X2 *
X3 *
[+] = present, [*] = absent
Table 4:-Identified evidences
a. Fungal growth on
strawberry
b. Rhizopus stolonifer fungi
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Volume 3 Issue 1
Table 5:-Observation
Months X0 X1 X2 X3
Control
1-4 months *
[*] = totally infected.
Chart-1: %of preservative efficacy of essential oils.
preservative efficacy of
essential oils.
S0
S-1
S-2
S-3
[S0] = X0, [S-1] = X1, [S-2] = X2, [S-3] =X3
As per study X0 specimen haven’t All three essential oils have strong and
preservative effects in pack condition. It beneficial preservative efficacy (Table-6).
less in amount inhibited contamination. Preservative efficacy ratio expected;
Level of barrier to infection showed cinnamon oil (25%): clove oil (25%):
medium in between in X1 and X2 and eucalyptus oil (50%)
strongly highest in X3 as shown in Chart 1.
Table 6:-Result
Strawberry X0 X1 X2 X3
Level of barrier to Very less Medium in Medium in Strongly
infection between between highest
Preservative efficacy No Yes Yes Yes
CONCLUSION showed by eucalyptus oil and medium in
In line with preservation, the result of this between preservative efficacy showed by
study showed that essential oils were seen cinnamon and clove oil. Some essential oils
to be the most preservative oils were are used as preserve some historical things,
cinnamon, clove and eucalyptus oils. plants parts, animal parts and organs parts.
Strongly highest preservative efficacy Preservative effects mainly depend on
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Volume 3 Issue 1
essential oils chemical constituents. The A. (2020). The effects of clove seed
natural essential oils have very strong (Syzygium aromaticum) dietary
bioactivities but they also have fine administration on carcass
preservatives. According to study best characteristics, meat quality, and
preservative oils are cinnamon, clove and sensory attributes of broiler
eucalyptus essential oils. “Preservative chickens. Poultry Science.
strawberries are not able to eat but their 7. Wankhede, T. B. (2015). Evaluation of
size, structure and their seeds are antioxidant and antimicrobial activity
maintained by essential oils. Strawberries of the Indian clove Syzygium
have colour changes due to strong activity aromaticum L. Merr. and Perr. Int Res
of essential oils. It employed as a specimen J Sci Eng, 3, 166-72.
preservative for research study, 8. Bhowmik, D., Kumar, K. S., Yadav,
morphological study and literature study. A., Srivastava, S., Paswan, S., & Dutta,
It’s good specimen preservatives.” And it A. S. (2012). Recent trends in Indian
survives up to 4 months no any kind of traditional herbs Syzygium
contamination and infection found. aromaticum and its health
benefits. Journal of Pharmacognosy
REFERENCES and Phytochemistry, 1(1), 13-22.
1. Yousef, N., Niloufar, M., & Elena, P. 9. Pino, J. A., Marbot, R., Agüero, J., &
(2019). Antipathogenic effects of Fuentes, V. (2001). Essential oil from
emulsion and nanoemulsion of buds and leaves of clove (Syzygium
cinnamon essential oil against aromaticum (L.) Merr. et Perry) grown
Rhizopus rot and grey mold on in Cuba. Journal of Essential Oil
strawberry fruits. Foods and Raw Research, 13(4), 278-279.
materials, 7(1). 10. Ramadan, M. F., Asker, M. M. S., &
2. Rao, P. V., & Gan, S. H. (2014). Tadros, M. (2013). Lipid profile,
Cinnamon: a multifaceted medicinal antiradical power and antimicrobial
plant. Evidence-Based properties of Syzygium aromaticum
Complementary and Alternative oil. Grasas y Aceites, 64(5), 509-520.
Medicine. J. Ecam., 2014. 11. El-Sayed, I. M., & El-Ziat, R. A.
3. Jeremić, K., Kladar, N., Vučinić, N., (2021). Utilization of environmentally
Todorović, N., Hitl, M., Lalić-Popović, friendly essential oils on enhancing the
M., & Gavarić, N. (2019). postharvest characteristics of
Morphological characterization of Chrysanthemum morifolium Ramat
cinnamon bark and powder available in cut flowers. Heliyon, 7(1), e05909.
the Serbian market. Biologia 12. Hermann, R., & von Richter, O.
Serbica, 41(1). (2012). Clinical evidence of herbal
4. Ravindran, P. N., Nirmal-Babu, K., & drugs as perpetrators of
Shylaja, M. (Eds.). (2003). Cinnamon pharmacokinetic drug
and cassia: the genus Cinnamomum. interactions. Planta medica, 78(13),
CRC press. 1458-1477.
5. A. Mbaveng, V. Kuete, Pages 611–625 13. Clarke, S. (2008). Composition of
in Syzygium Aromaticum, Medicinal essential oils and other
Spices and Vegetables from Africa, materials. Essential Chemistry for
Elsevier Inc, Amsterdam, Aromatherapy (Second Edition).
Netherlands (2017). Edinburgh: Churchill Livingstone,
6. Suliman, G. M., Alowaimer, A. N., Al- 123-229.
Mufarrej, S. I., Hussein, E. O., Fazea, 14. Lo Cantore, P., Shanmugaiah, V., &
E. H., Naiel, M. A., ... & Swelum, A. Iacobellis, N. S. (2009). Antibacterial
HBRP Publication Page 1-6 2021. All Rights Reserved Page 5Journal of Advances in Nanotechnology and its Applications
Volume 3 Issue 1
activity of essential oil components and activity of essential oil from leaves of
their potential use in seed Cinnamomum osmophloeum. Journal
disinfection. Journal of agricultural of Agricultural and Food
and food chemistry, 57(20), 9454- Chemistry, 53(18), 7274-7278.
9461.. 17. Deep R. Darji, Palak Sapra, Dr.
15. Dutra, R. C., Leite, M. N., & Barbosa, Archana U. Mankad. In-Vitro
N. R. (2008). Quantification of Evaluation Of The Inhibitory Effect Of
phenolic constituents and antioxidant Selected Natural Essential Oils On
activity of Pterodon emarginatus vogel Different Samples. International
seeds. International Journal of Journal of Creative Research Thoughts
Molecular Sciences, 9(4), 606-614. (IJCRT), 9(3): 4483-4488.
16. Chao, L. K., Hua, K. F., Hsu, H. Y.,
Cheng, S. S., Liu, J. Y., & Chang, S. T.
(2005). Study on the antiinflammatory
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