Neuroscience Antibodies & Protocols

Neuroscience Antibodies & Protocols

Neuroscience Antibodies & Protocols

Neuroscience Antibodies & Protocols (U.S.A. & European edition) 2006 www.abcam.com

Neuroscience Antibodies & Protocols

Catalogue contents Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience Contents Welcome to Abcam About Abcam ___ 1
Neuroscience at Abcam ___ 2
Neuroscience Abwire ___ 2
Abbreviations Species key ___ 5
Neuroscience antibody categories Cell adhesion proteins Cytoskeletal ___ 6
ECM ___ 8
Membrane ___ 10
Nuclear ___ 10
Growth & development Axonal Guidance ___ 11
Neurogenesis ___ 11
Neurotrophins ___ 12
Notch Pathway ___ 13
Signal Transduction ___ 14
Neurodegeneration Alzheimer’s ___ 18
Huntington’s ___ 22
Parkinson’s ___ 22
Prions ___ 24
Related Targets ___ 25
Neuroendocrinology GH Regulation ___ 26
Gonadotrophic Axis ___ 26
HPA Axis ___ 27
Obesity & Metabolism ___ 28
Technical help Neuroscience resources at Abcam ___ 64
Product datasheet guide ___ 65
Ordering and contact details Contact details ___ 89
Ways to order ___ 89
Coming soon New Neuroscience antibodies ___ 91
Target index Target index ___ 92
The team ___ 2
Benefits of an Abcam account ___ 3
AbreviewsSM and Abcam AbpointsSM ___ 3
Applications key ___ 5
Oxytocin & Vasopressin ___ 30
Thyroid Axis ___ 30
Neuronal markers Glia ___ 31
Neural Stem Cells ___ 33
Neuron ___ 35
Neurotransmission Calcium Signaling ___ 43
Intracellular Signaling ___ 44
Neurotransmitters ___ 47
Neuropeptides ___ 50
Nitric Oxide ___ 52
Pharmacological Tools ___ 53
Receptors & Channels ___ 53
Secretory Vesicles ___ 59
Transporters ___ 61
Sensory pathways Hearing ___ 62
Nociception ___ 62
Olfaction ___ 63
Touch ___ 63
Vision ___ 63
Neuroscience protocols and tips ___ 66
Neuroscience signaling pathway diagrams ___ 85
Required Information for ordering ___ 89
Abcam’s terms and conditions .

Neuroscience Antibodies & Protocols
1 Welcome to Abcam Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM) About Abcam Abcam, located in Cambridge, U.K. and Cambridge, MA, U.S.A., was founded in 1998 by Dr. Jonathan Milner, then a researcher at Cambridge University, U.K.: “Like many life science researchers, I was frustrated by the time it took to locate and select antibodies essential for my research. This was largely due to poor information and out-of-date catalogs from the vast range of suppliers who were spread across many countries. In some cases, I also experienced difficulties with companies whose products were unreliable and whose customer service was slow and unhelpful. My vision was to build a company that offered reliable cutting-edge products and great customer service.” It was a tough vision - Abcam was created to sell the best antibodies in the world with the most comprehensive, honest and up-to-date datasheets, fast delivery, helpful customer service and comprehensive technical support. Seven years on and with thousands of products across our range, Abcam ships to scientists in over 55 countries around the world. Abcam is committed to the following fundamental principles that have seen us become the first choice for thousands of life-scientists throughout industry and academia:
  • Quality of products and datasheets
  • Helpful technical support and online technical resources
  • Friendly customer service
  • Fast delivery Comprehensive We have a policy of honesty - our customers know everything that we know about our products, as soon as we know it. Everything we know about each product is posted on the datasheet. To achieve exceptional levels of quality for our customers we provide:
  • Comprehensive datasheets.
  • Customers’ AbreviewsSM of our products .
  • Product-specific protocols used by our customers.
  • Customers’ technical questions and our answers for each product.
  • Product information and new products updated daily. Helpful Our Technical Support and Customer Service departments are full of scientists that have many years of experience working with the types of products that we supply to our customers. We provide continuous order progress updates, so that you always know what is happening with your order and when you should expect to receive it.

The Queen's Award for Enterprise The Queen's Award for Enterprise has been running since 1966 and is widely recognised as the premier award for business performance in the UK; granted annually for outstanding business achievement.

Abcam demonstrated excellent year-on-year growth in overseas sales between 2002 and 2004 with 84% of sales during these three years coming from overseas customers. The award is positive proof of the company's outstanding success as a customer-focused international business. Welcome to Abcam ...all at www.abcam.com!

Neuroscience Antibodies & Protocols

Free services The World’s Antibody Gateway search engine If we do not have the product that you are looking for use this service to search all online catalogs of other antibody suppliers. Find the “WAG” at: www.abcam.com/antibodygateway The Abcam Toolbar Directly search your choice of Abcam, GenBank, Google, LocusLink, OMIM, PubMed, SWISSProt. The toolbar sits within your internet browser, so that you can use it from wherever you are on the internet. Get this useful facility at: www.abcam.com/toolbar Fast We have developed web-based systems that enable us to quickly identify cutting-edge products, make them rapidly available to our customers and then deliver them by Express Delivery.

We are pleased to be able to offer next-day delivery to all major cities as well as same day delivery to customers in Cambridge U.K. and Cambridge MA, U.S.A. Neuroscience at Abcam Our range of over 2,000 Neuroscience primary antibodies has a breadth and depth that is hard to beat. Further more, we collaborate with leading academics to make the Abcam range one of the best in the world! The Neuroscience Abwire Our Neuroscience Abwire web page is full of exclusive articles, product browsing tools, conference and publication information and specialist protocols. Visit today for cup-to-date information in this rapidly progressing field.

Look out for new Neuronal Marker IHC Galleries, Neurodegeneration and Neurotransmission signaling pathway diagrams and Neuroscience Product Locator tools (to name but a few).

Visit: www.abcam.com/neuroscience for more. The Neuroscience team Abcam has a specialized team working on sourcing and testing the best Neuroscience antibodies available. Our background in the neurosciences is very strong to better serve our customers. Members include: Technical Support Sarah Mardle (PhD King’s College, University of London, U.K.) Technical Support Manager (U.K.) Bill Campbell (PhD Brown University, U.S.A.) Technical Support Manager (U.S.A.) 2 Welcome to Abcam Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience Sarah and Bill help customers who have enquiries about, or problems with, Abcam antibodies using their extensive PhD and postdoctoral laboratory experience.They also:
  • Attend conferences to keep up-to-date with antibody techniques and research
  • Source new protein targets
  • Source novel techniques using antibodies
  • Build collaborations with academics.

3 Welcome to Abcam Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM) Neuroscience at Abcam The Neuroscience team Business Development Sabrina Edmonds (PhD University of Cambridge, U.K.) Business Development Manager for Neuroscience Sabrina is responsible for sourcing new and exciting protein targets to generate antibodies against by attending conferences, forging collaborations with academics and by reading current literature. She is the main contact for academics who are interested in licensing their neuroscience antibodies for sale through Abcam.

  • Marketing Rhian Hayward (PhD University of Oxford, U.K.) Neuroscience Marketing Manager Rhian is responsible for marketing Abcam’s Neuroscience range to our customers worldwide. She ensures that Abcam supports many Neuroscience community activities through conference sponsorships, informative marketing materials e.g. signalling pathways (see page 83), the Neuroscience Abwire and more. Suggestions or comments: Email the team at neuroscience@abcam.com Benefits of an Abcam Account Take full advantage of all our online Neuroscience resources by opening an account.
  • Earn Abcam AbpointsSM
  • Buy quickly and efficiently online.
  • Customise your antibody updates. We can notify you by email of new and updated antibodies related directly to the protein families which interest you.

AbreviewsSM Our unique system for enabling our customers to feedback to you and us about the performance of our primary antibodies. It is important to know how our antibodies perform under different conditions and that they are working as expected. We encourage this feedback (positive and negative) by awarding AbpointsSM for each Abreview: Customers’ Abreviews are posted on the product datasheets for others to browse. You can contact the reviewer to ask further questions too! AbreviewTM Action Points Description Submit an Abreview 50 Each unique combination of antibody, application and sample species that you review, earns you Abcam AbpointsSM , e.g.

if you use one of our antibodies in WB on Human and Mouse samples and submit an Abreview for both these combinations, then you will receive 50 Points for each one. Submit an Abreview (untested application / species) 200 As for “Submit an Abreview” but for an antibody that has not yet been tested in that particular species or application.

Submit an image with your Abreview 100 If you submit an image to accompany any of your Abreviews, then you will receive extra Points when it is published with your Abreview. For Abreview and image publication criteria, please see the Abreviews section of our website. Fast Track Abreviews bonus 950 Be the 1st researcher to submit conclusive positive or negative data (through Abreviews see below) about one of our Fast Track antibodies and earn this bonus (in addition to the Abcam Points that you will earn for submitting your Abreview).

  • 4 Welcome to Abcam Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience Abcam AbpointsSM In addition to AbreviewsSM , you can also earn AbpointsSM by buying your antibodies online at www.abcam.com or by registering your interests there. AbpointsSM rewards
  • Vouchers for leading retailers:
  • Useful gifts:
  • Discounts on Abcam products:
  • Money to go to conferences / for journal subscriptions:
  • Donate money to charities: ...You can even suggest new rewards. Collecting and spending Read about collecting and spending AbpointsSM and get the complete list of rewards for your country at: www.abcam.com/abpoints. Check out the latest at www.abcam.com

5 Key Species Many wide range of species At Arabidopsis thaliana Am Amphibian Av Avian Ba Baboon Bu Burkholderia spp. Ca Cat Ce Caenorhabditis elegans Ch Chicken C Cow De Deer D Dog Dm Drosophila melanogaster El Elk Ec Escherichia coli Key Species Fe Ferret Fi Fish Gt Goat G Goose Gp Guinea pig Ha Hamster H Horse Hu Human In Insect K Kangaroo Li Lizard Mm Mouse Ma Mammals Mi Mink Mk Monkey Op Opossum Key Species Pi Pig P Plants Q Quail Rb Rabbit Rt Rat Re Reptiles Ro Rodents Sc Sacchromyces cerevisiae S Salamander Sha Shark Sh Sheep Sn Snake X Xenopus laevis Y all Yeast Abbreviations Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A.

1 617 225 2272 (Toll free: 1 888 77 ABCAM) Key Applications AF Acetone fixed Agg Agglutination AI Adhesion inhibition AP Affinity purification BL Blocking CCIe Counter current immunoelectrophoresis ChIP Chromatin immunoprecipitation Conjugation Conjugation DID Double immunodiffusion Dot Dot blot EC Experimental control EIA Enzyme immunoassay ELISA ELISA EM Electron microscopy EMSA Electrophoretic mobility shift assay FACS Fluorescence activated cell sorting FC Flow cytometry FuncS Functional studies GSA Gel supershift assays H Histology I-ELISA Indirect ELISA IA Immunoassay IB Immunoblotting ICC Immunocytochemistry ID Immunodiffusion Ie Immunoelectrophoresis IF Immunofluorescence IHC Immunohistochemistry IHC-F Immunohistochemistry (Formalin fixed sections) IHC-Fr Immunohistochemistry (Frozen sections) Key Applications IHC-P Immunohistochemistry (Formalin-fixed paraffin-embedded sections) IM Immunomicroscopy Inhib Inhibition assay IP Immunoprecipitation IRMA Immunoradiometric assay ISH In situ hybridization Mct Microcytotoxicity testing Neut Neutralizing P Purification PA Prothrombin assay PCC Primary cell culture PCR Polymerase chain reaction RIA Radioimmunoassay RID Radial immunodiffusion RIe Rocket immunoelectrophoresis RImm Radial immunodiffusion RipA Radioimmunoprecipitation assay SB Southern blot TCC Transfected cell culture TLC Thin layer chromatography WB Western blotting Other Fast Track Application and species data pending Pep.

avail. Peptide available Abbreviations Species key Applications key Many of the images in this catalog have been supplied by Abcam’s collaborators in the Neuroscience field. We wish to thank all these contributors and we look forward to working with you in the future!

6 Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Intermediate Filaments Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... GARP (truncated) P WB Rb Rt, B ab15094 GFAP P IF, IHC-Fr Ch Hu, Mm, Rt, F, C ab4674 GFAP P IF, IHC-F, IHC-P, IP, PCC, WB Rb F, B, Hu, Mm, Pi, Rt ab7260 GFAP P IF, IHC-F, IHC-Fr, IHC-P Rb Hu, Rt, B ab7779 GFAP [2A5] M IF, IHC-F, IHC-P, PCC, WB Mm Ma ab8136 GFAP [6F2] M IHC-Fr, IHC-P, IM, TEM immunogold labeling, WB Mm Hu ab8975 GFAP [GF-01] M ICC, IHC-Fr, IHC-P, IP, WB Mm Hu, F, Pi Does not react with Mm or Rt ab7806 GFAP (phospho T7) [YC10] M ICC, WB Mm Hu, C, Pi Does not react with Mm or Rt ab12340 Internexin alpha P ICC, IHC-Fr, WB Rb Hu, Mm, Rt, C, F ab22038 Internexin alpha [1D2] M ICC, IHC-P, WB Mm Hu, Rt ab22039 Internexin alpha [2E4] M ICC, IHC-Fr, IHC-P, WB Mm Hu, Rt ab22040 Laminin P ELISA, IF, IHC-P, RIA Rb Hu ab23753 Laminin P ELISA, WB Ch Hu, Mm, Rt ab14055 Laminin P Dot, IF, IHC-F, IHC-P Rb Ma, Am, Av, Re ab11575 Laminin [A5] M ICC, IHC-Fr, WB Rt Hu, Mm, Ba ab2500 Laminin [LAM-89] M IF, IHC-P Mm Hu, F, Rb.

Does not react with Mm, Rt, Ch, D, Gp, Li, Pi, Sh or Sn ab11574 Laminin [MEC5] M IHC-Fr Rt Hu, Mm, Rt ab2466 Laminin 2 alpha [4H8-2] M ELISA, ICC, IF, IP, WB Rt Hu, Mm ab11576 Laminin 5 P IHC-Fr, WB Rb Hu ab14509 Laminin B2 gamma 1 [SPM277] M IF, IHC-P, IP, WB Rt Hu, Mm ab17792 Laminin S [C4] M IP, WB Mm Hu, Rt, B, Pi, Gp, Ch ab3289 67kDa Laminin Receptor P ICC, IF, IHC-P, IP, WB Rb Hu, Mm ab711 67kDa Laminin Receptor [MLuC5] M FACS, ICC, IF, IHC-P Mm Hu, Rt ab3099 Nestin P ICC, IF Rb Hu, Ha, Mk Does not react with Mm ab5968 Nestin [10C2] M IF, IHC-Fr, IHC-P, WB Mm Hu. Does not react with rodent nestin ab22035 Nestin [2C13A11] M FACS, ICC, IP, WB Mm Hu.

Does not react with Rt ab18102 Nestin [2Q178] M EM, ICC, IHC-Fr, IHC-P, WB Mm Mm, Rt Does not react with Hu, F, Mk or Sh ab6142 Nestin [3k1] M ELISA, FACS, ICC, IHC (aldehyde-based fixation), IP, WB Mm Hu ab6320 68kDa Neurofilament [1D2] M ICC, IF, IHC-Fr, IHC-P, WB Mm Hu, Rt ab4571 68kDa Neurofilament [NR4] M IF Mm Hu, Rt, Ch, Pi ab11219 68kDa Neurofilament [SPM204] M IHC-P, WB Mm Hu ab17832 70 kDa Neurofilament Light P IHC-F, IHC-Fr, IHC-P, WB Rb Hu, Mm, Rt, B, Pi, Av ab9035 70 kDa Neurofilament Light [2F11] M IHC-Fr, IHC-P, WB Mm Hu, Op ab8970 70 kDa Neurofilament Light [DA2] M ICC, IF, IHC-Fr, WB Mm Hu, Mm, Rt, Ch, C, Pi ab4572 160 kDa Neurofilament Medium P IF, IHC-F, IHC-Fr, IHC-P, WB Rb Hu, Mm, Rt, F, B, Pi, Re, Av ab9034 160 kDa Neurofilament Medium [3H11] M IHC-F, IHC-Fr, IHC-P, WB Mm Hu ab7256 160 kDa Neurofilament Medium [RNF403] M IHC-Fr, WB Mm Hu, Rt ab9271 160 kD Neurofilament Medium antibody [NF-09] (ab7794) IF - ab7794 at a dilution of 1/1000, staining on rat brain tissue (30µm thick coronal sections) in free floating IHC.

Clonality Applications tested Host Species reactivity Datasheet M WB, ELISA, IHC-P, IHC-Fr, IF, ICC Mm Ma www.abcam.com/ab7794 Cell adhesion proteins Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience

7 200 kDa Neurofilament Heavy P IF, IHC-Fr, IHC-P, WB Rb F, B, Hu, Mm, Pi, Rt ab8135 200 kDa Neurofilament Heavy [NAP4] M ELISA, IHC-F, IHC-Fr, IHC-P, WB Mm Av, Ma ab7257 200 kDa Neurofilament Heavy [RNF402] M IHC-Fr, IHC-P, WB Mm Hu, Rb, Rt, Mm, B, D, Sh, Gp, Ch, Ha, Mk, X ab3966 200 kDa Neurofilament Heavy [SPM203] M IHC-P, WB Mm Hu ab17879 200kDa + 70kDa Neurofilament [2F11] M IF, IHC, IHC-P, IP, WB Mm Hu ab17114 Peripherin P ELISA, ICC, IF, IHC-F, IHC-P, WB Rb Ma ab7258 Peripherin [2Q135] M ICC, WB Mm Hu, Mm, Rt, C, Pi. Does not react with Ch ab17999 Peripherin [7C5] M ICC, IHC-P, WB Mm Ch ab4573 Peripherin [8G2] M ELISA, ICC, IHC-F, IHC-P, WB Mm Ma ab7657 Peripherin variant PER56 P IHC-Fr, IHC-P, WB Rb Hu, Mm ab4655 Peripherin variant PER61 P IHC-Fr, IHC-P, WB Rb Hu, Mm ab4646 Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Microtubules Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

beta III Tubulin [TU-20] M ICC, IF, IHC-P, IP, WB Mm Ma ab7751 beta III Tubulin [TUJ-1] M ICC, IF, IHC-Fr, WB Mm Hu, Rt ab14545 Doublecortin P IHC-Fr Gp Hu, Mm, Rt ab10293 MAP2 P IF, IHC, WB Ch B, Hu, Mm, Rt ab5392 MAP2 [AP-20] M ICC, WB Mm Mm, Rt, X, B, Q, S ab11268 MAP2 [HM-2] M ICC, IHC, WB Mm Hu, Mm, Rt, B, Ch, Q ab11267 MAP2 [MT-01] M ICC, IF, IP, WB Mm Mm, F, C, Pi ab7756 MAP2a + MAP2b [AP20] M ICC, IF, IHC-P, WB Mm Hu, Rt, Mm, B, Ch, X , Q ab3096 MAP2a + MAP2b [MT-01] M ELISA, IHC-Fr, IHC-P, WB Mm Hu, Mm, F ab8479 MAP5 [3G5] M IF, IHC-P, IHC on primary cultures Mm Hu, Mm, Rt, C. Does not react with Ch ab3095 Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Regulation Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

Thrombospondin 1 [CSI 002-65] M ELISA, IHC, IHC-Fr, IP Mm Hu, B ab23420 Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Thin Filaments Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... MYRIP P Fast track Gt Fast track ab10149 Pep. avail. 200 kD Neurofilament Heavy antibody [NF-01] (ab7795) IF - ab7795 at a dilution of 1/1000, staining in rat brain tissue (30 µm thick coronal sections) on free floating IHC. Clonality Applications tested Host Species reactivity Datasheet M WB, ELISA, IHC-P, IHC-Fr, IF, ICC Mm Ma www.abcam.com/ab7795 Cell adhesion proteins Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A.

1 617 225 2272 (Toll free: 1 888 77 ABCAM) Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Intermediate Filaments Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

8 Neuroscience > Cell Adhesion Proteins > ECM Proteins Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... Agrin [AGR 131] M ICC, IHC, IRMA, WB Mm Mm, Rt ab12362 Agrin [Agr 247] M ICC, IF Mm Mm, Rt ab12364 Agrin [Agr 86] M FACS, ICC, WB Mm Mm, Rt ab12361 Amisyn P Fast track Gt Fast track ab5899 Pep. avail. Bestrophin P ICC, IHC, IP, WB Rb Hu, Mm, Rt ab14927 Bestrophin M WB Mm Hu, Pi, Mk ab2182 Drebrin P WB Rb Rt, Hu ab11068 Drebrin [M2F6] M IHC-Fr, IHC-P, WB Mm Hu, Mm, Rt, Ch, F, Gp, Mk ab12350 Fetuin A P ELISA, WB Rb Hu ab13980 Laminin P ELISA, IF, IHC-P, RIA Rb Hu ab23753 Laminin P ELISA, WB Ch Hu, Mm, Rt ab14055 Laminin P Dot, IF, IHC-F, IHC-P Rb Ma, Am, Av, Re ab11575 Laminin [A5] M ICC, IHC-Fr, WB Rt Hu, Mm, Ba ab2500 Laminin [LAM-89] M IF, IHC-P Mm Hu, F, Rb.

Does not react with: Mm, Rt, Ch, D, Gp, Li, Pi, Sh or Sn ab11574 Laminin [MEC5] M IHC-Fr Rt Hu, Mm, Rt ab2466 Laminin 2 alpha [4H8-2] M ELISA, ICC, IF, IP, WB Rt Hu, Mm ab11576 Laminin 5 P IHC-Fr, WB Rb Hu ab14509 Laminin B2 gamma 1 [SPM277] M IF, IHC-P, IP, WB Rt Hu, Mm ab17792 Laminin S [C4] M IP, WB Mm Hu, Rt, B, Pi, Gp, Ch ab3289 67kDa Laminin Receptor P ICC, IF, IHC-P, IP, WB Rb Hu, Mm ab711 67kDa Laminin Receptor [MLuC5] M FACS, ICC, IF, IHC-P Mm Hu, Rt ab3099 Mint3 P WB Rb Rt, Mm ab3450 Pep. avail.

NCAM [123C3] M IF, IHC-F, IHC-Fr, IHC-P Mm Rt, Hu ab8077 NCAM [B332 (123A8)] M FACS, IHC-Fr, WB Mm Hu ab8596 NCAM [ERIC-1] M ELISA, IHC-Fr, IP, RIA, WB Mm Hu ab6123 NCAM [H28-123] M AP, IHC-Fr, IM, WB Rt Mm ab19782 NCAM [MEM-188] M FACS, IP, WB Mm Hu ab8233 NCAM [RNL-1] M ICC, IF, IHC-Fr, WB Mm Hu, Mm, Rt ab9018 Nicastrin P WB Rb Hu, Mm ab3444 Pep. avail. SIRP [MRC OX-41 ] M FACS, IHC-Fr, IHC-P, WB Mm Rt ab9295 SIRP alpha P IHC-P, WB Rb Hu, Mk. Does not react with Mm ab2971 SIRP alpha P WB Rb Hu, Rt, Mm ab8120 Pep. avail. Reelin antibody [142] (ab18569) IHC-P - ab18569 detecting Reelin immunoreactivity in Cajal-Retzius cells of the mouse cortex at postnatal day 0.

ab18569 was used at 1/1000 (overnight incubation at 4°C). Immunoreactivity was visualized using ABC amplification. Clonality Applications tested Host Species reactivity Datasheet M ELISA, ICC, IHC-P, IP Mm Many www.abcam.com/ab18569 67kD Laminin Receptor antibody (ab2508) IF - staining observed in the hippocampus in the cytoplasm of some astrocytes and neurons using ab2508. Recommended dilution of antibody is 1:300-1:1000 with overnight incubation at room temperature. Free floating IHC performed with TSA amplification.

Clonality Applications tested Host Species reactivity Datasheet P IHC-Fr, Fl, IP, WB Rb Hu www.abcam.com/ab2508 Cell adhesion proteins Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience

9 Synaptotagmin P ICC, IP, WB Rb Rt, X ab13260 Synaptotagmin P IF, WB Rb Hu, Rt ab10104 Synaptotagmin P ELISA, WB Ch Hu, B, Mm, Pi, Rt ab8037 Synaptotagmin [ASV30] M ICC, IHC, IP, WB Mm Mm, Rt, X, B, Ch, Rb, Sha ab13259 Synaptotagmin [ASV48] M ICC, IHC, IP, WB Mm Rt, B, Mk, Sha, Mm ab12255 SynCAM P WB Rb Rt ab3910 Syntrophin [1351] M IF, IP, WB Mm Hu, Mm, Rt, Am, Ch, D, Fi ab11425 Syntrophin gamma 2 P ELISA, WB Gt Hu ab15713 Pep.

avail. Tenascin C P WB Ch Hu ab16290 Tenascin C [MTn-12] M AF, Dot, ELISA, IF, IHC, IHC-F, IHC-Fr, IP, WB Rt Mm ab6346 Reelin antibody [G10] (ab18570) IHC-P - ab18570 detecting Reelin immunoreactivity in Cajal-Retzius cells of the mouse cortex at postnatal day 0. ab18569 was used at 1/5000 (overnight incubation at 4°C). Immunoreactivity was visualized using ABC amplification. NB: Antigen retrieval performed (microwave in citrate buffer pH 6).

Clonality Applications tested Host Species reactivity Datasheet M WB, IP, ELISA, IHC-Fr, ICC, IHC-P Mm Mm, Rt. Does not react with Hu www.abcam.com/ab18570 Cell adhesion proteins Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM) Neuroscience > Cell Adhesion Proteins > ECM Proteins Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... Neuroscience Abwire at www.abcam.com/ neuroscience

10 Neuroscience > Cell Adhesion Proteins > Membrane Proteins Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

Cathepsin B P ID, WB Sh Hu ab211 Cathepsin B P IHC-Fr, IHC-P Rb Hu ab7670 Cathepsin B [3E1] M IHC-Fr, IHC-P Mm Hu ab7430 Cathepsin D P ELISA, WB Rb Hu ab19555 Cathepsin D P IHC-Fr, IHC-P Rb Hu ab826 Cathepsin D [CTD-19] M ELISA, IHC-F, IHC-P, WB Mm Hu ab6313 Cathepsin D [D101] M IHC-Fr, IHC-P Mm Hu ab7433 Cathepsin G P ELISA, WB Rb Hu ab20725 Cathepsin G P IHC-Fr, IHC-P Rb Hu ab15988 Cathepsin G P WB Sh Hu ab8816 Cathepsin H [1D10] M IHC-Fr, IHC-P, WB Mm Hu ab7432 Cathepsin L P IHC-Fr, IHC-P Sh Hu ab7454 Cathepsin L [33/2] M ELISA, IHC-Fr, WB Mm Hu, Mm, Rt, Mi ab6314 Heparan Sulfate Proteoglycan 2 [CSI 001-74] M AP, ELISA, IHC, IHC-Fr, IP, WB Mm Hu, C ab23418 Heparan Sulfate Proteoglycan 2 [SPM255], prediluted M IHC-P Rt Hu, Mm, B, Pi ab17848 MOSP [CE1] M IF, IHC-Fr Mm Hu, Rt, Mm, F, Ch, Mk, Ca ab3094 Myelin Basic Protein P WB Rb Hu, Mm, Rt ab12355 Myelin Basic Protein P IF, IHC-Fr, IHC-P Rb Hu, Rt ab2404 Myelin Basic Protein [2B5] M ICC, IHC-F, IHC-Fr, IHC-P Mm Hu ab8053 Myelin Basic Protein [12] M AF, ELISA, IF, IHC-Fr, PCC, RIA, WB Rt Hu, B, Gp, Mm, Rb, Rt, Sh ab7349 Myelin Basic Protein [26] M IHC-Fr Mm MBP from most species ab11162 Myelin Basic Protein [B505] M IHC-Fr, IHC-P Mm Hu, Rb, Rt, C, Mk ab8764 Myelin Basic Protein [Clone 2] M ELISA, IHC-Fr Mm Hu, Rt, B, Gp, Rb, Sh ab11159 Myelin PLP [plpc 1] M IF, IHC-Fr, WB Mm Ma ab9311 PMP22 P IHC-P Rb Hu ab15506 PMP22, prediluted P IHC-P Rb Hu ab15507 PMP22 [20-14] M IHC-P Mm Hu ab3278 SORL1 P Fast track Gt Fast track ab22814 Spinesin P WB Gt Mm ab2245 Proteins Myelin Basic Protein protein (Biotin) ab792 Neuroscience > Cell Adhesion Proteins > Nuclear Proteins Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

Galectin 3 P IHC, WB Rb Hu ab2473 Galectin 3 [A3A12] M ELISA, FACS, IF, IHC-Fr, IP, WB Mm Hu, Rt, Mm ab2785 Galectin 7 P IP, WB Rb Mm ab10482 NrCAM antibody (ab24344) IF - ab24344 (1/300) detects NrCAM on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM. Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Clonality Applications tested Host Species reactivity Datasheet P IF, IHC-F, IHC-P, IHC-FrFl, IP, WB Rb Hu, Mm, Rt www.abcam.com/ab24344 Cell adhesion proteins Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience

11 Neuroscience > Growth and Development > Axonal Guidance Proteins Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... Aggrecan ARGxx [BC-3] M ELISA, IHC-Fr, IHC-P, WB Mm Hu, B, F, D, Gp, H, Pi, Rb, Rt, Sh ab3773 AMIGO2 P WB Rb Mm, Rt ab16762 CRMP5 [CR-1] M IHC-Fr, WB Rt Hu, Rt, B ab19378 CRMP5 [CR-3] M IHC-Fr, WB Rt Hu, Mm, Rt, B ab19352 EphA1 P ELISA, WB Rb Hu ab5385 EphA1 P I-ELISA, WB Gt Hu ab7036 EphA2 P ELISA, WB Rb Ha, Hu, Mm ab5387 EphA2 P BL, ELISA, ICC, IHC-Fr, WB Gt Mm ab10610 EphA3 P I-ELISA, WB Gt Mm ab7038 EphA3 P ELISA, WB Gt Hu. Does not react with Mm ab10611 EphA3 [III-A4] M FACS, IP Mm Hu ab19439 EphA4 P ELISA, WB Rb Hu ab5389 EphA4 P I-ELISA, WB Gt Mm ab7039 EphA4 P I-ELISA, WB Gt Hu ab7041 EphA5 P ELISA, WB Rb Hu, Mm ab5397 EphA5 P ELISA, WB Gt Mm, Rt ab10612 EphA6 P ELISA, WB Gt Mm ab10613 EphA7 P ELISA, WB Rb Hu ab5411 EphA7 P ELISA, WB Gt Mm ab10614 EphA8 P ELISA, WB Gt Mm ab10615 EphB1 P ELISA, WB Rb Hu, Mm ab5414 EphB1 P I-ELISA, WB Gt Rt ab7042 EphB1 P Dot, IP Sh Hu ab10406 EphB2 P ELISA, WB Rb Hu, Mm ab5418 EphB2 P Dot, ELISA Gt Mm ab10616 EphB3 P ELISA, WB Gt Mm ab10617 EphB3 P ELISA, WB Gt Hu ab7044 EphB4 P Dot, ELISA, Inhib Gt Mm ab10618 EphB6 P Dot, ELISA Gt Mm ab10619 L1CAM [UJ127] M IF, IHC-P, IP, WB Mm Hu ab3200 L1CAM [UJ12711] M IP, WB Mm Hu ab20148 L1CAM [UJ1814] M IP, WB Mm Hu ab20149 Neuropilin 1 P ELISA, WB Gt Hu ab18130 Neuropilin 1 P IHC-P, WB Rb Rt, Mm ab16786 Neuroserpin P IHC, WB Rb Hu ab16171 NRG1 P WB Rb Hu, Mm ab2994 NRG1 [7D5] M IHC-Fr, IHC-P, IP, WB Mm Rt, Mm ab2369 NRP2 P WB Ch Hu ab15874 Reelin [142] M ELISA, ICC, IHC-P, IP, WB Mm Ma, Av ab18569 Reelin [G10] M ELISA, ICC, IHC-Fr, IHC-P, IP, WB Mm Mm, Rt.

Does not react with Hu ab18570 Robo1 P ELISA, IF, WB Rb Mm ab7279 Robo4 P WB Rb Mm ab10547 Slit1 P WB Rb Mm, Rt ab10984 Slit2 P Fast track Rb Fast track ab7665 Proteins Robo4 protein ab19112 Growth and development Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM)

12 Neuroscience > Growth and Development > Neurogenesis Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... Axotrophin P WB Ch Hu, Mm, Rt ab13997 Cdk5 P ELISA, WB Rb Hu, Mm, Rt ab19426 Cdk5 [SPM236], prediluted M IHC-P Mm Hu, Mm, Rt ab17875 DYRK1A P IF, IP, WB Sh Hu ab16140 PTPR P WB Rb Hu ab12153 STMN2 P Fast track Gt Fast track ab21190 Neuroscience > Growth & Development > Neurotrophins Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

Artemin P WB Rb Hu ab1136 BDNF P ELISA, WB Rb Hu ab9793 BDNF [35928.11] M ELISA, WB Mm Hu ab10505 BDNF (Biotin) P ELISA, WB Rb Hu ab12385 BMP2 P Dot, ELISA, WB Rb Hu, Mm, Rt ab14933 BMP2 P ELISA, WB Rb Hu ab17885 BMP2 [65529.111] M I-ELISA, WB Mm Hu ab6285 BMP2 (Biotin) P ELISA, WB Rb Hu ab17597 CNTF P I-ELISA, Neut, WB Gt Rt ab10834 CNTF P ELISA, Neut, WB Rb Rt ab9785 CNTF P ELISA, IHC, WB Ch Hu, Mm, Rt ab17692 CNTF [AS23] M ELISA, IP, WB Mm Hu ab17284 CNTF (Biotin) P ELISA, WB Rb Rt ab12426 GDNF P I-ELISA, Neut, WB Gt Hu ab10835 GDNF P Dot, IHC-Fr, WB Sh Hu ab6206 GDNF P ELISA, IHC, WB Ch Hu, Mm, Rt ab17637 GDNF family Receptor alpha 2 P IHC-Fr, WB Rb Mm, Rt ab6170 GDNF family Receptor alpha 3 P IHC-Fr, WB Rb Mm, Rt ab6171 GDNF family Receptor alpha 3 P WB Rb Hu, Rt, Mm ab8028 Pep.

avail. GDNFR alpha P WB Rb Hu, Rt, Mm ab8026 Pep. avail. GFR alpha 2 P WB Rb Hu, Mm, Rt ab19146 BDNF antibody (ab6201) IHC - immunostaining BDNF-containing hippocampal neurons in coronal rat brain sections (30 microns). ab6201 used at 1/100 dilution.

Clonality Applications tested Host Species reactivity Datasheet P Dot, ELISA, IHC-Fr, Neut, WB Rb Hu, Rt www.abcam.com/ab6201 FOX1A antibody (ab12161) ICC - ab12161 staining (1/200) in mouse E11 cortical cells cultured for 6 days. Clonality Applications tested Host Species reactivity Datasheet P WB, IF, ICC, IHC Rb Hu, Mm www.abcam.com/ab12161 Growth and development Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience

13 GFR alpha 3 P WB Rb Hu, Mm, Rt ab19135 LANGF Receptor [MC-192] M FACS, IHC-Fr Mm Rt ab6172 Neurotrophin 3 P Dot, ELISA, IHC-Fr, Neut, WB Rb Hu ab6202 Neurotrophin 3 P ELISA, IHC-Fr, IHC-P, WB Gt Hu, Rt ab10334 Neurotrophin 3 (Biotin) P ELISA, WB Gt Hu ab17524 Neurotrophin 4 P ELISA, Neut, WB Gt Hu ab9824 Neurotrophin 4 M ELISA Mm Hu ab9330 Neurotrophin 4 (Biotin) P ELISA, WB Rb Hu ab17888 Neurotrophin 4/5 P Dot, ELISA, IHC-Fr, Neut, WB Rb Hu ab6205 Neurturin P Dot, ELISA, IHC-Fr, WB Sh Hu ab6208 Neurturin P WB Rb Hu ab8061 Neurturin peptide BL ab8393 NGF P Inhib, Dot, ELISA, IHC-Fr, Neut, WB Rb Mm ab6198 NGF [1.A.15] M ELISA Mm Hu ab18167 NGF 2.5S P Dot, Inhib, WB Rb Hu, Mm, Ch ab10515 NGF beta P ELISA, Neut, WB Gt Hu, Mm, Rt ab10837 NGF beta [25623.1] M Dot, ELISA, Inhib Mm ab10513 NRG1 P WB Rb Hu, Mm ab2994 NRG1 [7D5] M IHC-Fr, IHC-P, IP, WB Mm Rt, Mm ab2369 p75 NGF Receptor P Dot, FACS, IF, IHC-Fr, IHC-P, IP, WB Rb Mm, Rt ab8874 p75 NGF Receptor [12A8] M Dot, ICC, IHC-Fr, IP, WB Rt Mm ab8876 p75 NGF Receptor [ME20.4] M EM, ELISA, FACS, ICC, IF, Mm Hu, F, Pi .

IHC-P, Inhib, IP, RIA, WB Does not react with Mm, Rt or Ch ab10495 p75 NGF Receptor [NGFR5] M FACS, IF, IHC-P Mm Hu, Ba, F, Ca, Mk, Rb Does not react with Mm or Rt ab3125 Pleiotrophin P ELISA, WB Rb Hu ab14025 Pleiotrophin P ELISA, WB Gt Hu ab10849 SPON1 P WB Ch Hu, Mm, Rt, C ab14271 TrkA (phospho Y490) P ICC, IP, WB Rb Hu, Mm, Rt ab1445 TrkA/B [1.A.37] M IP, WB Mm Hu ab18137 TrkB P IHC-Fr Rb Rt ab6180 TrkB P I-ELISA, WB Gt Hu ab10841 Proteins BDNF protein ab9794 CNTF protein ab9786 GDNF protein ab9790 Neurotrophin 3 protein ab9792 Neurotrophin 4 protein ab9825 Neurturin protein ab9840 NGF beta protein ab9796 TrkA antibody (ab8871) IF - ab8871 immunostaining in terminals of primary afferent fibers (fibers in the top part of the figure) of the dorsal root ganglion.

Free floating IHC performed on 30um cryostat sections. Primary antibody ab8871 was used at 1/3000 and incubated overnight at room temperature.

Clonality Applications tested Host Species reactivity Datasheet P IHC-Fr, IHC-FrFl, IF Rb Rt www.abcam.com/ab8871 Growth and development Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM) Neuroscience > Growth & Development > Neurotrophins Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

14 Neuroscience > Growth and Development > Notch Pathway Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... ADAM10 P WB Rb Hu ab10956 APH1a P WB Rb Rt, Hu, Mm ab12104 Pep.

avail. APH1a P IF, IP, WB Rb Hu, Mm ab19390 DLL4 P ELISA, IHC-F, IHC-P, WB Rb Hu, Mm ab7280 Jagged 1 P ELISA, FACS, Neut, WB Gt Hu, Rt ab10580 Jagged 2 P Fast track Rb Fast track ab10556 LNX P Fast track Gt Fast track ab4538 MAML1 P Fast track Gt Fast track ab17019 Pep. avail. Notch1 [A6] M FACS, IHC-P, WB Mm Hu, Mm ab3294 Notch1 (activated) P Co-IP, ICC, IF, IP, WB Rb Hu, Mm ab8925 Pep. avail. Notch2 (activated) P Fast track Rb Fast track ab8926 Pep. avail. Notch1 intra P WB Rb Hu.

Does not react with Mm ab8387 Pep. avail. Notch1 [SPM206], prediluted M IHC-P Mm Hu, Mm ab17843 Notch1 intra + Notch2 intra P Fast track Rb Fast track ab8928 Pep. avail. Notch 2 intra P WB Rb Hu ab8927 Pep. avail. NUMB P IF, IHC-P, IM, WB Gt Hu, Mm ab4147 Pep. avail. NUMB P IP, WB Rb Hu, Mm ab14140 Pep. avail. NUMBL P Fast track Gt Fast track ab5641 Pep. avail. PEN2 P IP, WB Rb Hu ab16555 Presenilin 1 P ELISA, IHC-Fr, IHC-P, WB Gt Hu ab7297 Presenilin 1, prediluted P IHC-P Rb Hu ab15508 Presenilin 1 [APS 11] M ELISA, IF, IHC-P, WB Mm Hu, Mm, Rt ab15456 Presenilin 1 [APS 18] M ELISA, IF, WB Mm Hu, Mm, Rt ab15458 Presenilin 2 P ELISA, IHC-Fr, IHC-P Gt Hu ab11148 Presenilin 2 P ELISA, IHC, WB Ch Hu, Mm, Rt ab18041 Presenilin 2 [198C67921] M WB Mm Hu ab13926 Presenilin 2 [APS 26] M ELISA, IF, WB Mm Hu, Mm ab15549 SIAH Interacting Protein P Fast track Gt Fast track ab4276 Pep.

avail. SIAH1 P WB Gt Hu ab2237 Pep. avail.

Neuroscience > Growth and Development > Signal Transduction Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... 14-3-3 P IP, WB Rb Hu, Mm, Rt ab9063 14-3-3 P IHC-P, IP, WB Rb Hu, Mm, Rt ab6081 14-3-3 [2Q248] M WB Mm Rt ab14121 14-3-3 [3F7] M IP, WB Mm Hu ab14108 14-3-3 beta P IHC-P, IP, WB Rb Hu, Mm, Rt ab15260 14-3-3 beta P WB Rb Hu, Mm, Rt ab16730 14-3-3 beta [60C10] M ELISA, WB Mm Hu, Mm, Rt ab16859 14-3-3 beta, prediluted P IHC-P Rb Hu, Mm, Rt ab15262 14-3-3 beta + epsilon + zeta [3C8] M WB Mm Hu, Mm, Rt ab12346 14-3-3 beta + zeta P WB Rb Ma, Dm ab14113 14-3-3 beta + zeta [22-IID8B] M ICC, IHC, WB Mm Hu, Mm, Rt, B, D, Mk, Pi, Rb, Sh ab12347 14-3-3 eta + gamma + zeta P ELISA, WB Rb Many ab14124 14-3-3 gamma (acetyl V1) [KC21] M WB Mm Hu ab17005 14-3-3 gamma [3F8] M IP, WB Mm Hu ab14117 14-3-3 sigma [1N6] M IF, IHC-P, IP, WB Mm Hu ab14123 14-3-3 tau [3B9] M IP, WB Mm Hu, Mm, Rt, B ab10439 14-3-3 Binding Motif (phospho S) P ELISA, IHC-P, IP, WB Rb Many ab14127 14-3-3 Binding Motif (phospho S) [3i2] M ELISA, IP, WB Mm Many ab14126 Agrin [AGR 131] M ICC, IHC, IRMA, WB Mm Mm, Rt ab12362 Agrin [Agr 247] M ICC, IF Mm Mm, Rt ab12364 Agrin [Agr 86] M FACS, ICC, WB Mm Mm, Rt ab12361 AKT P WB Ch Hu, Mm, Rt, X , B ab13990 AKT P ICC, IP, WB Rb Hu, Mm, Rt ab6076 Growth and development Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience

15 AKT (unmodified S473) [BDI111] M ELISA, WB Mm Hu ab19442 AKT (phospho S473) P IF Rb Hu, Mm ab20488 AKT (phospho T308) P IF, WB Rb Hu ab8933 AKT (phospho T34) P ELISA, WB Rb Hu, Mm, Rt, Rb ab23509 AKT peptide BL ab9041 CrkL P WB Gt Hu, Mm ab10907 CSN7b P WB Rb Hu ab11895 Ctip1 [14B5] M ICC, IF, IHC, IP, WB Mm Hu, Mm, Fi ab19487 Ctip1 [15E3AC11] M ICC, IF, IHC, IP, WB Mm Hu, Mm ab18688 Ctip1 [18B12DE6] M ICC, IF, IHC, IP, WB Mm Hu, Mm ab19489 AKT (phospho T308) antibody (ab4796) IHC - ab4796 at a dilution of 1/100 (2 overnight incubations at room temperature; ABC amplification). Cytoplasmic and nuclear staining observed in rat spinal cord motorneurons (ventral horn) 30µm saggital spinal cord sections.

Clonality Applications tested Host Species reactivity Datasheet P WB, IHC Rb Hu, Mm, Rt www.abcam.com/ab4796 AKT (phospho S473) antibody (ab4802) IF - detection of AKT phospho S473 at a dilution of 1/200 (24h incubation at RT). Perinuclear staining observed in ventral horn neurons of rat spinal cord (30µm saggital sections).

Clonality Applications tested Host Species reactivity Datasheet P WB, IHC-P, IHC-Fr, IF, ICC, IHC Rb Hu, Mm, Rt www.abcam.com/ab4802 AKT (phospho S473) antibody (ab8932) IF - AKT is phosphorylated on Ser 473 in breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue. The phospho Ser 473 AKT antibody (ab8932) is used with no pretreatment at a dilution of 1:100. Clonality Applications tested Host Species reactivity Datasheet P WB, IHC-P, IF Rb Hu www.abcam.com/ab8932 AKT antibody (ab8805) IF - ab8805 used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct.

AKT staining (green) and actin filaments (red).

Clonality Applications tested Host Species reactivity Datasheet P WB, IHC-P, IF, ICC, IP, Rb Hu www.abcam.com/ab8805 Transfected Cell Culture, PCC Growth and development Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM) Neuroscience > Growth and Development > Signal Transduction Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

16 Dab1 P IP, WB Gt Mm ab16674 Dab1 P IF, IHC-Fr, IP, WB Rb Mm ab7522 Dab1 (phospho S491) P WB Rb Hu ab5776 Dab1 (phospho Y198) P WB Rb Hu ab5673 Dab1 (phospho Y220) P WB Rb Hu ab5677 Dispatched P Fast track Gt Fast track ab10142 ELAVL2 + ELAVL3 + ELAVL4 [16A11] M IHC-Fr, WB Mm Hu, Mm, Rt, Fi ab14370 EMX2 P IHC, WB Rb Hu, Mm, Rt ab11849 Fetuin A P ELISA, WB Rb Hu ab13980 FMR1 (Drosophila) [6A15] M ELISA, IF, IP, WB Mm Dm.

Does not react with Hu ab10299 FOXG1 P WB Gt Hu ab3394 Pep. avail. FOXG1 P IHC-P, WB Rb Hu ab23470 GCNF P IHC-P Rb Hu ab13007 Gemin 1 [2B1] M ELISA, IF, IP, WB Mm Hu, Mm, X ab5831 Gemin 2 [2E17] M ELISA, IF, IP, WB Mm Hu, X ab6084 Gemin 3 [12H12] M ELISA, IF, IP, WB Mm Hu ab10305 Gli1 P IHC-P, IP, WB Rb Hu, Mm ab7523 Gli2 P Fast track Rb Fast track ab7181 Gli2 P Fast track Rb Fast track ab7195 Gli3 P Fast track Rb Fast track ab6050 LIM Kinase P WB Rb Mm ab10561 LIS1 P IP, WB Rb Hu ab2607 LIS1 P WB Gt Mm ab2656 Pep. avail. MAP4K6 P IHC-P Rb Hu ab13140 NCAM [123C3] M IF, IHC-F, IHC-Fr, IHC-P Mm Rt, Hu ab8077 NCAM [B332 (123A8)] M FACS, IHC-Fr, WB Mm Hu ab8596 NCAM [ERIC-1] M ELISA, IHC-Fr, IP, RIA, WB Mm Hu ab6123 NCAM [H28-123] M AP, IHC-Fr, IM, WB Rt Mm ab19782 NCAM [MEM-188] M FACS, IP, WB Mm Hu ab8233 NCAM [RNL-1] M ICC, IF, IHC-Fr, WB Mm Hu, Mm, Rt ab9018 Neuro D4 P Fast track Gt Fast track ab3940 Pep.

avail. Neuroglobin P ELISA, WB Ch Hu ab14010 NR2E3 P IHC-P Rb Hu ab13081 NRG1 P WB Rb Hu, Mm ab2994 NRG1 [7D5] M IHC-Fr, IHC-P, IP, WB Mm Rt, Mm ab2369 PAX2 P IHC-P, WB Rb Hu ab23799 PAX6 P WB Rb Rt, Sh ab5790 Pep. avail. PHOX2A P IHC, IP, WB Rb Hu, Mm, Rt ab12046 PHOX2A P Fast track Gt Fast track ab4511 PHOX2B P IHC, IP, WB Rb Rt, Hu, Mm ab12047 PROX1 P ELISA, IHC-Fr Rb Hu, Mm, Rt, Ch, Fi ab11941 RAI3 P ICC, IHC-P Rb Hu ab13274 ROR alpha P WB Rb Hu ab16367 ROR alpha P IHC-P Rb Hu ab13109 ROR beta P IHC-P Rb Hu ab13113 SCP1 P IF Rb Hu, Mm ab15087 SCP1 P IF, IHC-Fr, IHC-P Rb Mm ab15090 Ctip2 antibody [25B6] (ab18465) IHC - using ab18465 on adult mouse hippocampus.

Clonality Applications tested Host Species reactivity Datasheet M WB, IP, IHC-P, IHC-Fr, IF, Rt Hu, Mm, Fi www.abcam.com/ab18465 ICC, IHC, ChIP Growth and development Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience Neuroscience > Growth and Development > Signal Transduction Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

17 SIRP alpha P IHC-P, WB Rb Hu, Mk. Does not react with Mm ab2971 SIRP alpha P WB Rb Hu, Rt, Mm ab8120 Pep. avail. SIRP [MRC OX-41 ] M FACS, IHC-Fr, IHC-P, WB Mm Rt ab9295 Slit1 P WB Rb Mm, Rt ab10984 Slit2 P Fast track Rb Fast track ab7665 SOX2 P IF Rb Hu, Mm ab15830 Pep. avail. SOX6 P IHC, IP, WB Rb Hu, Mm, Rt ab12054 SOX8 P IHC, IP, WB Rb Hu, Mm, Rt ab12055 SOX13 P IHC, IP, WB Rb Hu, Mm, Rt ab12060 SOX14 P IHC, IP, WB Rb Hu, Mm, Rt ab12061 SOX22 P IHC, IP, WB Rb Hu, Mm, Rt ab12062 Sprouty 2 P ELISA, IP, WB Rb Hu ab1043 SynCAM P WB Rb Rt ab3910 Tenascin C P WB Ch Hu ab16290 Tenascin C [MTn-12] M AF, Dot, ELISA, IF, IHC, IHC-F, IHC-Fr, IP, WB Rt Mm ab6346 TRAF6BP P WB Rb Hu ab22049 VCAM1 [14C3] M IF, IHC-P Mm Hu, Mm ab11514 VCAM1 [1G11B1] M FACS, IA, ICC, IHC-Fr, IP Mm Hu ab7219 VCAM1 [M/K-2] M FuncS, IF, IHC Rt Mm ab19569 VCAM1 [MVCAMA(429)] M FACS Rt Mm ab23807 Zic1 P IHC, IP, WB Rb Hu, Mm, Rt ab12070 Zic2 P IHC, WB Rb Hu, Mm, Rt ab12072 Growth and development Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A.

1 617 225 2272 (Toll free: 1 888 77 ABCAM) Neuroscience > Growth and Development > Signal Transduction Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

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18 Neuroscience > Neurodegeneration > Alzheimer’s > Amyloid Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/... ADAM10 P WB Rb Hu ab10956 AMPK alpha 1 P IP, WB Rb B, Hu, Rt ab3759 Amyloid A Component [mcl], prediluted M IHC-Fr, IHC-P Mm Hu ab7505 Amyloid beta Precursor Protein P ELISA, IHC-Fr, IHC-P, WB Gt Hu ab17468 Amyloid beta Precursor Protein P ICC, IP, WB Rb Hu, Mk ab12268 Amyloid beta Precursor Protein P ICC, IHC-P, IP, WB Rb Hu, Mk ab12269 Amyloid Precursor Protein P ELISA, IHC-P, WB Gt Hu ab17325 Amyloid Precursor Protein P IHC-P, IP, WB Rb Hu, Mk ab11117 Amyloid Precursor Protein P WB Rb Hu, Mm ab2073 Amyloid Precursor Protein P ELISA, IHC-Fr, IHC-P, WB Gt Hu ab2084 Amyloid Precursor Protein (phospho T668) P ICC, IHC-P, IP, WB Rb Hu, Mm, Rt ab17417 Amyloid Precursor Protein [3E9] M IHC-P, WB Mm Hu ab12338 Amyloid Precursor Protein Frameshift Mutant P ELISA, IHC-P, WB Gt Hu ab2085 Amyloid Precursor Protein peptide BL ab7874 APH1a P IF, IP, WB Rb Hu, Mm ab19390 APH1a P WB Rb Rt, Hu, Mm ab12104 Pep.

avail. beta Amyloid 1-40 P ELISA, IHC-Fr, RIA Rb Hu ab16218 beta Amyloid 1-40 P IP, WB Rb Hu, Mm ab16622 beta Amyloid 1-40 P ELISA, IHC-P, WB Rb Hu ab10147 beta Amyloid 1-40 [3H2] M ELISA, IF, IHC-P, WB Mm Hu ab17331 beta Amyloid 1-40 [BAM-10] M IHC-Fr, IHC-P Mm Hu ab7501 beta Amyloid 1-40 [BDI350] M ELISA, ICC, WB Mm Hu ab20068 beta Amyloid 1-42 P ELISA, IHC-P, WB Rb Hu ab10148 beta Amyloid 17-42 P ELISA Rb Hu ab17327 beta Amyloid P ELISA, IHC, WB Ch Hu ab17472 beta Amyloid P IHC Rb Ro ab14220 beta Amyloid P ELISA, WB Rb Hu, Mm ab2539 beta Amyloid [2C8] M IHC, WB Mm Hu, Mm ab12349 beta Amyloid [4G8] M ELISA, IHC-P, IP, WB Mm Hu ab1910 beta Amyloid peptide BL ab3698 CLACP P WB Rb Hu, Mm ab16763 Factor VIII heavy chain [RFFVIIIC/8] M ELISA, WB Mm Hu, Pi.

Does not react with Mm, Rt or D ab6345 beta Amyloid antibody [6E10] (ab10146) IHC-Pusing ab1910 [clone 4G8] and ab10146 [clone 6E10]. Stains paraffin - embedded cerebellum. Clonality Applications tested Host Species reactivity Datasheet M WB, IP, ELISA, IHC-P Mm Hu www.abcam.com/ab10146 Amyloid Precursor Protein antibody [MABDE2B4] (ab12266) IHC-Pab12266 detecting APP in Alzheimer’s disease human brain. Plaques are observed in the neuropil. Amyloid deposits stained by DE2B4 (ab12266) can be seen around some blood vessels.

Clonality Applications tested Host Species reactivity Datasheet M WB, IP, IHC-P, ICC, Mm Hu, C, Mk, Pi www.abcam.com/ab12266 Neurodegeneration Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience

19 FE65 P WB Rb Mm ab5668 Pep. avail. FE65 [4H324] M ICC, IP, WB Mm Hu, Mm, Rt ab17469 Humanin P WB Rb Hu ab16758 Mint1 P WB Rb Rt ab3448 Pep. avail. Mint3 P WB Rb Rt, Mm ab3450 Pep. avail. PEN2 P IP, WB Rb Hu ab16555 RAGE P ELISA, IHC-P Gt Hu ab20558 RAGE P IHC-Fr, WB Rb Rt, Mm ab3611 SHC P IP, WB Rb Hu, Mm, Rt ab15039 SHC (phospho Y239 + Y240) P WB Rb Hu ab4890 Pep.

avail. SHC (phospho Y317) P WB Rb Hu, Mm ab15040 Neuroscience > Neurodegeneration > Alzheimer’s > Related targets Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

Acrolein P ELISA Rb Acrolein ab15138 Caspase 6 (active) P WB Rb Mm, Rt, Hu ab2326 ADAM17 P Dot, WB Rb Hu, Rt, Mm ab2051 Pep. avail. ADAM17 P WB Rb Hu, Rt, Pi ab11547 ADAM17 P WB Gt Hu ab13535 Pep. avail. Als2 P Fast track Gt Fast track ab4155 Pep. avail. ALS2CR1 P WB Gt Hu ab4123 Pep. avail. ALS2CR2 P WB Gt Hu ab4122 Pep. avail. ALS2CR2 P WB Rb Hu, Mm, Rt ab17205 ALS2CR3 P Fast track Gt Fast track ab4200 Pep. avail. Apolipoprotein E P ELISA, IHC-P, WB Gt Hu ab7620 Apolipoprotein E P ID, IE, RID, WB Rb Mm ab20874 Apolipoprotein E [E6D7] M ELISA, IHC-Fr, IHC-P, IP, WB Mm Hu ab1907 Calpastatin P ELISA, WB Ch Hu ab16423 Calpastatin [2G11D6] M ICC, WB Mm Hu, Rt, C, Pi ab3515 Caspase 6 P WB Rb Hu, Mm, Rt ab2552 Caspase 6 P ELISA, WB Rb Hu, Mm, Rt ab18520 Caspase 6 P WB Rb Hu ab19920 Caspase 6 [3F51] M IHC-P, IP, WB Mm Hu ab17866 Caspase 6 [3F52] M IHC-P, IP, WB Mm Hu ab17822 Caspase 6 [6CSP01 (7165)] M IP Mm Hu ab3245 Caspase 6 [6CSP03] M WB Mm Hu ab4062 CCKAR P WB Rb Hu ab14441 Drebrin P WB Rb Rt, Hu ab11068 Drebrin [M2F6] M IHC-Fr, IHC-P, WB Mm Hu, Mm, Rt, Ch, F, Gp, Mk ab12350 ERAB P ELISA, IF, IHC-P, WB Rb Hu ab17298 ERAB [5F3] M Dot, ELISA, IF, IHC-Fr, IHC-P, WB Mm Hu ab10260 Fyn P ELISA, WB Gt Hu ab802 Pep.

avail.

ApoE antibody [D6E10] (ab1906) IHC-P - ab1906 reactivity with Apo E of vascular amyloid beta in a Down Syndrome tissue section. Clonality Applications tested Host Species reactivity Datasheet M WB, IHC-P, IHC-Fr Mm Hu www.abcam.com/ab1906 Neurodegeneration Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM) Neuroscience > Neurodegeneration > Alzheimer’s > Amyloid Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

20 Fyn P WB Rb Hu, Mm ab13955 Fyn [1S] M IHC-P Mm Hu, Rt, Mm ab3116 Fyn [FYN-01] M ICC, IP, WB Mm Hu, Mm ab1881 PHF2 P WB Rb Hu ab1138 Synuclein (alpha) P H, ICC, IHC, IHC-Fr, IHC-P, WB Sh Hu ab6162 Synuclein (alpha) P IHC-P Rb Hu, Rt ab15530 Synuclein (alpha) P ICC, WB Gp Hu, Mm, Rt, B ab16784 Synuclein (alpha) (phospho Y125) P Fast track Rb Fast track ab10789 Pep.

avail. Synuclein (alpha) [4B12] M Dot, ELISA, IHC-Fr, IHC-P, WB Mm Hu. Does not react with Mm or Rt ab1904 Synuclein (alpha) [4D6] M ELISA, IHC-Fr, IHC-P, WB Mm Hu, Mm, Rt ab1903 Synuclein (beta) P ICC, IHC-Fr, IHC-P, WB Rb Hu ab6165 Synuclein (beta) P IHC-Fr, IHC-P Sh Hu ab6163 Synuclein (beta) P WB Ch Hu ab16420 Synuclein (beta) P IHC-P Rb Hu, Rt ab15532 Synuclein (gamma) P IHC, IHC-Fr, IHC-P, WB Rb Hu, Rt ab6169 Synuclein (gamma) P IHC-Fr, IHC-P, WB Rb Hu, Rt ab6176 Synuclein (gamma) P IHC-P, WB Rb Hu, Rt ab15534 Ubiquitin P ELISA, IF, IHC-F, IHC-Fr, IHC-P, IP, WB Rb Hu, Rt, H, Mm, B ab7780 Ubiquitin P ELISA, WB Rb Y ab14372 Ubiquitin P WB Ch Hu ab19310 Ubiquitin [10C2-2] M AP, ELISA, IHC-Fr, IHC-P, WB Mm All ubiquitinated proteins ab411 Ubiquitin [1B4-UB] M ELISA, FACS, IHC-Fr, IHC-P, WB Mm Hu, B ab122 Neuroscience > Neurodegeneration > Alzheimer’s > Proteases Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

BACE1 P IHC-P Gt Hu ab11028 BACE1 P WB Rb Hu, Rt, Mm ab2077 Pep. avail. BACE2 P WB Rb Hu, Rt, Mm ab8025 Pep. avail. BACE peptide BL ab5857 Cathepsin H [1D10] M IHC-Fr, IHC-P, WB Mm Hu ab7432 Nicastrin P WB Rb Hu, Mm ab3444 Pep. avail. Presenilin 1 P ELISA, IHC-Fr, IHC-P, WB Gt Hu ab7297 Presenilin 1, prediluted P IHC-P Rb Hu ab15508 Presenilin 1 [APS 11] M ELISA, IF, IHC-P, WB Mm Hu, Mm, Rt ab15456 Presenilin 1 [APS 18] M ELISA, IF, WB Mm Hu, Mm, Rt ab15458 Presenilin 2 P ELISA, IHC, WB Ch Hu, Mm, Rt ab18041 Presenilin 2 P ELISA, IHC-Fr, IHC-P Gt Hu ab11148 Presenilin 2 [198C67921] M WB Mm Hu ab13926 Presenilin 2 [APS 26] M ELISA, IF, WB Mm Hu, Mm, Rt ab15549 Ubiquitin antibody (ab6309) IF - P23H mutant cells immunostained with ab6309 (1/500 dilution) showing co-localization of mutant aggregated protein with ubiquitin.

Clonality Applications tested Host Species reactivity Datasheet P WB, IHC-Fr, IA, IF Rb C, Sc www.abcam.com/ab6309 Neurodegeneration Ordering information: www.abcam.com | Tech support: www.abcam.com/technical Neuroscience resources: www.abcam.com/neuroscience Neuroscience > Neurodegeneration > Alzheimer’s > Related targets Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

21 Neuroscience > Neurodegeneration > Alzheimer’s > Tangles & Tau Target Clonality Applications tested Host Species reactivity Datasheet www.abcam.com/...

14-3-3 tau [3B9] M IP, WB Mm Hu, Mm, Rt, B ab10439 Casein Kinase 1 delta P ELISA, IHC-P Gt Hu ab10877 ERK1 P IHC-P, IP, WB Rb Hu, Rt, Mm ab7947 Pep. avail. ERK1 P ELISA, IP, WB Rb Hu, Rb, Rt, Mm, C, Pi ab9363 ERK1 (HRP) P ELISA, IP, WB Rb Hu, B, Mm, Pi, Rb, Rt ab9364 ERK1 + ERK2 (phospho T185 + T202) P WB Rb Hu ab4819 Pep. avail. ERK1 + ERK2 (phospho T185 + T202) (FITC) P FACS, IHC-Fr, WB Rb Hu ab4820 ERK1 + ERK2 (phospho T202/183 + Y204/185) P WB Rb Hu, Mm, Rt ab16869 ERK1 + ERK2 (phospho T202 + Y204) P IF Rb Hu, Mm, Rt ab20490 ERK1 + ERK2 P IP Sh Hu, Rt ab16573 ERK1 + ERK2 P WB Rb Hu, Mm, Rt, B ab17942 GSK3 (alpha + beta) (phospho Y216 + Y279) P Dot, ELISA, WB Rb Hu, Mm, Rt ab4797 GSK3 (alpha + beta) P IHC-P, WB Rb Hu, Mm, Rt, Ch, Mk ab15314 GSK3 (alpha + beta) [1H8] M ELISA, WB Mm Hu, Mm, Rt, Am ab17059 Neurofibrillary Tangle P IHC-Fr, IHC-P, WB Rb Hu ab2076 Neurofibrillary Tangle P ELISA, IHC-P, WB Rb Hu ab11093 PEN2 P IP, WB Rb Hu ab16555 Pin1 P WB Rb Hu ab12107 Pep.

avail. Prealbumin P DID, Ie, RID, RIe Sh Hu, B, F, Ch, D, Gt, Gp, H, Mm, Pi, Rb, Rt ab9015 Prealbumin P IHC, RID Rb Hu, B, F, D, H ab16006 Prealbumin P ELISA Gt Hu ab15007 Tau (phospho S199) P WB Rb Hu ab4749 Pep. avail. Tau (phospho S199 + S202) P WB Rb Hu ab4864 Tau (phospho S202) P IHC-Fr, WB Rb Hu ab4839 Pep. avail. Tau (phospho S208) P WB Rb Hu ab10892 Pep. avail. Tau (phospho S214) P WB Rb Hu ab4846 Pep. avail. Tau (phospho S214) P Fast track Rb Fast track ab10891 Pep. avail. Tau (phospho S356) P WB Rb Hu ab4857 Pep. avail. Tau (phospho S262) P WB Rb Hu ab4856 Pep. avail. Tau (phospho S396) P WB Rb Hu ab4858 Pep.

avail. Tau (phospho S400) P WB Rb Hu ab4859 Pep. avail. Tau (phospho S404) P WB Rb Hu ab4860 Pep. avail. Tau (phospho S409) P WB Rb Hu ab4861 Pep. avail. Tau (phospho S422) P WB Rb Hu ab4862 Pep. avail. Tau (phospho T181) P WB Rb Hu ab4748 Pep. avail. Tau (phospho T212) P WB Rb Hu ab4842 Pep. avail. Tau (phospho T217) P WB Rb Hu ab4851 Pep. avail. Tau (phospho T231) P WB Rb Hu ab4855 Pep. avail. Tau P IHC-P, WB Gt Hu ab11107 Tau P IHC-F, WB Rb Hu ab22690 Tau P IF, IHC-Fr, IHC-P Rb Hu ab8763 Tau [T1] M WB Mm Hu, Mm, Rt, B ab12354 Tau [Tau] M IHC-Fr, IHC-P Mm Ch, B, Hu, Mk. Does not react with Mm or Rt ab8739 Tau [TAU-5] M IHC-Fr, IHC-P, WB Mm Hu ab3931 Tau antibody (ab4841) IF - Transgenic C.

elegans first larval stage nematode expressing human wild type Tau GFP (green) and stained with ab4841 (red). Neurons of the head, dorsa and ventral cords and tail region display phosphorylated Tau. Clonality Applications tested Host Species reactivity Datasheet P WB, IHC-Fr, IHC (no paRtffin) Rb Hu www.abcam.com/ab4841 whole animals Neurodegeneration Customer Service Tel: Europe +44 (0) 1223 696000 | U.S.A. 1 617 225 2272 (Toll free: 1 888 77 ABCAM)

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