Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family

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Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
SCIENCE

                      Surface Scanning Electron Microscopy
                      of Suri Alpaca Fiber and Other Members
                      of the Camel Family
By Andy and Dr. Cheryl Tillman

                                                     SEM scanning demonstrates that the            To provide a baseline to other members
                                                     cuticular cell length, height, and scale   of the Camel Family, 19 llama, eight
                                                     edge angle of suri alpaca fiber is         huacaya, six vicuña, five guanaco, and
                                                     measurably different from huacaya          two de-haired Bactrian camel samples
                                                     alpaca, cashmere, wool, and other          were also analyzed. Other specialty
                                                     members of the Camel Family.               fibers analyzed included one sample
                                                       The Washington State Disease Diag-       each of white angora rabbit, washed
                                                     nostic Lab (WADDL) in Pullman, WA          fawn mohair, and Bombay silk. Two
                                                     analyzed 35 suri alpaca fiber samples      samples each of Soft Rolling Skin®
                                                     with surface scanning electron micro-      (SRS) merino wool, and both domestic
                                                     scopy in February through November         (in the grease) and very fine, washed
                                                     2005 for the authors. The WADDL            white Chinese cashmere were tested.
                                                     normally uses the Electron Microscopy         SEM scanning demonstrates that the
                                                     and Imaging Center (EMIC) to               cuticular cell scale length, frequency,
                                                     identify virus and bacteria species for    height, and scale edge angle of suri
                                                     the state of Washington. Samples were      alpaca fiber is measurably different
Suri fiber is characterized by an indistinct scale   prepared by EM Supervisor Chris            from these other specialty fibers and
edge that is difficult to visualize even with        Davitt, Ph.D., who took two micro-         other members of the Camel Family.
digital imaging tools.                               graphs of each fiber sample.               Cuticular scale length is expressed as
                                                                                                the Mean Scale Frequency (MSF) per
     Terminology                                                                                100 micron (µ) field of view as meas-
                                                                                                ured by the SEM. High-luster suri
                                                                                                appears to be most similar to cashmere,
    Angstrom (Å): A unit of length, equal to one ten-millionth of a millimeter,
                                                                                                though it has an even longer and lower
    primarily used to express electromagnetic wavelengths.
    Compound Microscope: An optical microscope with two ocular lenses.                          scale height. This study has important
    Cuticular Cell Scale: The external-most structure of hair.                                  implications for the alpaca industry,
    Highly Evolved: a merino or huacaya that has a high frequency of crimp, or                  including AOBA and the AFCNA. Suri
    a suri with unusually high luster or tightly-penciled locks.                                alpaca breeders can now claim to pro-
    Mean Scale Frequency (MSF): The number of cuticle scales per 100 micron                     duce a natural fiber which has luster
    that are the mean of the data set.                                                          that is equal to or greater than cashmere.
    Micrograph: A digital photograph of the SEM sample.                                         Due to its very low cuticular scale
    Micron: 1/1,000,000 of a meter (1/10,000 of a millimeter, or about 1/25,000th of            height, both suri and huacaya breeders
    an inch).
                                                                                                can explain why their products have
    Scanning Electron Microscopy (SEM): Use of the electron microscope to
    view the surface characteristics of an object.
                                                                                                superior handle, compared to wool of
    South American Camelid (SAC): A literal translation of Camelidos Sud Amer-                  similar average fiber diameter (AFD).
    icanos, incorporating all the members of the Lama genus, including the
    alpaca, llama, guanaco, and vicuña.                                                         Sample Demographics
    Transmission Electron Microscopy (TEM): Use of the electron microscope                      Samples were collected from 35 suri
    to view an object on-end, rather than on the surface of the subject.                        alpacas, comprised of 20 male and
                                                                                                15 females. Thirty of the suris were

158 ■ Alpacas Magazine
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
Most of the straighter fiber samples had excep-
                                                                                      tional luster and a low MSF.

                                                                                      The narrow flat lock was highly correlated to
                                                                                      luster in this study.

offspring from the 1991, 1996, and         “Yocum-McColl” sample site used for
1998 Bolivian importations to the          laser scanning. The second phase of
USA. These animals were randomly           fiber samples had a slightly lower MSF
selected from our own herd based on        than the first. Twenty of the suri
whether we had fiber samples or fleeces    alpacas had a uniform age range of 11
saved from the previous year’s clip.       to 13 months at the time of sampling.
Three Australian suris were also tested.   Eight samples from Phase 2 were sec-
These were first-generation suris from a   ond or third fleeces, rather than virgin
suri x huacaya cross that were pheno-      fleeces (and these animals were three to
typically suri. Two more of our suris      five years old). Three of these could be
were from Peruvian origins. Fiber sam-     compared to the same animal’s virgin
ples were collected in two phases. The     fleece. MSF was within +/- 10% of the
first phase of 14 samples was collected    virgin fleece. No significant difference
from shorn virgin fleeces. The remain-     was noted between the first and subse-
ing (majority) of samples were taken       quent fleeces. The remaining seven         A well-locked suri in his second fleece, like
from unshorn suris using a uniform         animals were 21 to 25 months old.          this animal, typically had an MSF of 7-7.5.

                                                                                                                Spring 2006 ■ 159
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
According to ARI statistics, over half of      measure the length of each scale on a
                                                  all suri alpacas in the United States are         fiber, the height of scale, frequency of
                                                  colors other than white. About two-thirds         scale, angle of scale, and fiber diameter.
                                                  of this study were colored: eleven subjects       For the purposes of this article, the rel-
                                                  were white, eight brown, seven black,             atively simple International Wool Tex-
                                                  five fawns, three greys, and one beige.           tile Organization (IWTO) DTM-XX-97
                                                     The majority of samples (21) had a             methodology was used with the excep-
                                                  twisted style of lock, including several          tion of viewing the samples at 1,000 X
                                                  with a highly-evolved “pearl” lock of             magnification rather than 600 X. The
                                                  3-4 very small inter-twined locks. The            higher magnification was necessary for
                                                  style of lock ranged from cotted to               accurately measuring suri scale height,
                                                  “independent.” The remaining samples              which is almost impossible to measure
                                                  had either a narrow flat lock (3), a rela-        even with digital imaging tools. Wool
                                                  tively straight fleece that clung closely         has a scale height of
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
Image-J was much more stable on our         Commercialization of SEM?
Windows XP platform than the                A used SEM costs USD$50,000. Add-
Macintosh-based Scion Image, which is       ing the other peripherals can raise the
an industry standard in SEM labs.           price to USD $250,000. Training to
   Surface SEM of suri alpacas was first    prepare the samples and take micro-
conducted in the United States in 1998      graphs would take at least a term of
at the University of Idaho at Moscow,       college-level classes in statistics and
by Suvia Judd and Deborah Berman.2          imaging software. SEM is a powerful
Judd and Berman analyzed two fiber          tool that can help breeders determine
samples and hypothesized a high corre-      the inherent luster of their most valuable
lation between scale length and luster.     animals. But it is unlikely that SEM
SEM of South American Camelid (SAC)         will ever be as common a test procedure
fiber has been published at least three     as laser scanning for determining AFD.
times prior to Judd and Berman in 1988      The cost of the equipment is just too
(Phan), 1996 (Antonini et al), and 1997     great. Cuticular scales can be visualized
(IWTO). Antonini’s study is particularly    by an inexpensive optical microscope
important. Extracting the data on just      by coating the fiber samples with a lac-       A bright fleece like this has a fan-shaped rather
Peruvian suris from that of Argentine       quer that enhances the contrast of the         than a twisted lock.
SAC with “Lustre” established that          scales. While sample preparation may
Peruvian suris have a MSF of 7.5. This      be as time-consuming as SEM, this is a
is equal to cashmere. The 1997 IWTO         much less expensive method of docu-
study of specialty fibers is one of many    menting MSF. Unfortunately, the lac-
which have tried to find an economical      quer greatly exaggerates the cuticular
method of testing large quantities of       scale height of the sample. Since SAC
cashmere for purity. The very low MSF       fiber has a very low scale height, SEM
of cashmere, compared to wool, makes        is still the preferred method of analyz-
SEM an effective tool to identify           ing surface scale structure.
blended shipments of cashmere.
   The author’s sample of suri and          Luster
specialty fibers is the largest domestic    Luster is the primary, and probably
analysis conducted to date.                 the only, reason the textile industry
                                            purchases suri alpaca fiber.
Sample Preparation                             Suri can be used in many of the same
Dr. Davitt prepared the fiber sample by     applications as silk and cashmere. It is
cutting the center 13mm (½ inch) out        frequently blended with merino, silk,
                                                                                           Luster is the primary reason the textile industry
of the locks. Dr. Davitt believed using     or cashmere to add luster to the fabric
                                                                                           purchases suri fiber. This brushed suri coat by
the center of each sample was prefer-       used in men’s suits.                           Beatriz Canedo Patiño is made from 100% suri.
able to one close to the skin or near the      “Luster is strongly associated with mo-
tip, and I concurred. The fiber sample         hair, based on its relatively large sur-
is mounted on an aluminum disc with            face cuticle scales and low cuticle scale
electrically-conductive tape and               edge height relative to merino wool.” 3
screwed into place on the sample
dish. A SEM dish holds twelve                  The end-use of suri alpaca fiber is sub-
13mm sample discs.                          stantially different than Baby Huacaya,
   The fiber samples were placed in         which can compete for fineness with
a vacuum chamber and coated with            some grades of merino wool. Suri is
20-30 angstroms of pure gold. A high        more likely to be used in a semi-
voltage is applied in a vacuum chamber      worsted or woolen manufacturing
to a bar of 99.9% pure gold, which          process and huacaya in a worsted yarn
coats the fiber samples with a plasma       like merino. Our study of eight hua-
of gold ions. The SEM is actually           caya alpaca samples demonstrated that
visualizing the gold ions that coat the     huacaya breeders can selectively breed
sample, not the fiber sample itself.        for enhanced brightness in their fleece.
Platinum and aluminum can also              A reduction in MSF of 25-30% should
be used to coat the samples.                be relatively easy to accomplish.

                                                                                                                     Spring 2006 ■ 161
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
Lock                                          emphasis on an “independent lock,”
                                                    It would be premature to assign value         than any other characteristic, including
                                                    to one lock type over another until a         luster or conformation. Our SEM
                                                    more thorough SEM is made of suri             study did not identify any correlation
                                                    alpaca fiber.                                 between a twisted lock and luster. If
                                                       The authors had hoped to identify          anything, they may be inversely pro-
                                                    some correlation between lock types,          portional. In this study, the relatively
                                                    which naturally occur in the suri alpaca,     straight fleeces, and those with a nar-
                                                    and the length of scale which is highly       row straight lock usually had a longer
                                                    correlated to luster. Our sample size         and lower scale height than animals
                                                    was too small to conclusively identify a      with twisted locks. Suri breeders, the
                                                    trend. However, it is probably accurate       AOBA Show Committee, and the
                                                    to say that a straighter fleece is more       Judges Training Committee should
                                                    likely to have a very low MSF than a          keep abreast of SEM technology as it
                                                    twisted lock. A literature search suggests    applies to suri alpaca fleeces. It would
                                                    that some lock types may actually inhib-      certainly be premature to favor one
MSF 4.6. Second and third fleeces had a very        it perceived luster more than others.         lock type over another until the results
low MSF. Pictured is a 3 month re-growth on            For instance, a high frequency of          of TSN 100-fleece study is completed.
"Rewinds" third fleece. There is no reason not
to show shorn suri’s in AOBA halter classes!
                                                    crimp in cashmere does adversely affect
                                                    the perceived luster of the fiber.            Scale Height
                                                       “Perceived luster of wool is affected by   The scale height of suri alpaca fiber
                                                       staple structure and fiber curvature.      was almost impossible to measure,
                                                       Low curvature in wool allows the fibers    even with digital imaging tools. It is
                                                       to more closely align. As Khan (1996)      essentially a mono-filament, like silk.
                                                       reported, if the staple crimp form in         The height of scale on suri alpaca fiber
                                                       wool was ‘planar’ (sinusoidal as           was almost impossible to measure, even
                                                       opposed to helical), such wool would       with digital Image-J software. This char-
                                                       have a high luster. On this basis, the     acteristic is probably as significant to the
                                                       use of perceived luster as an aid in the   textile industry as suri’s very low MSF.
                                                       classifying of cashmere may be con-        The scale height of both suri and hua-
Twenty-one of the 35 suri samples were from            founded by different cashmere fiber        caya fiber has not been accurately report-
well locked individuals like this. A twisted lock      curvature. Thus luster of cashmere         ed in previous scientific literature, which
probably does reduce luster compared to a              should be assessed on manually             is probably due to the fact that an opti-
straighter fleece.
                                                       straightened fiber to minimize any         cal rather than SEM has been used.
                                                       effect of fiber crimp.” 4                     “The smoothness of alpaca and mohair
                                                                                                     compared to wool is due to the scales
                                                       Applying McGregor’s findings to suri          being around half the height of wool at
                                                    alpacas may suggest that a tightly twist-        around the same micron.” 10
                                                    ed (helical) lock may diffuse light more
                                                    than a straighter fleece. It may also            While Mr. Sporle made an important
                                                    make the handle feel coarser than it          and accurate observation about the
                                                    actually is. Analysis of the Baby Camel       handle of alpaca and mohair, scale
                                                    supports the notion that crimp and/or         height is much lower than “one-half
                                                    a twisted lock may reduce luster in           the height of wool.” In our study, it
                                                    SAC fiber.                                    was one-tenth the height! Huacaya
                                                       “Wool with larger fiber crimp ampli-       fiber had slightly taller scale height
                                                       tude is associated with softer handle.     than suri, but was still typically under
                                                       Wools exhibiting a coiled (helical)        3/10µ. The most highly evolved Soft
                                                       crimp configuration receive harsher        Rolling Skin (SRS) merino fiber like
                                                       handle scores than wools with sinu-        that analyzed in this study had a scale
                                                       soidal (wavy) crimp configurations.” 3     height of 3-4 micron, and most merino
                                                                                                  is
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
Compare the lack of scale height of this suri   The low scale height of suri can clearly be seen   The scale edge of suri is so indistinct that it
sample to that of merino or cashmere (see       in this micrograph which shows three damaged       was difficult to measure even with digital
photos on p. 168-169).                          cuticle scales. Notice how thin they are!          imaging tools.

  “The greater the directional friction         appeared to be elliptical. One vicuna
  effect due to the wool fiber cuticle          had distinct grooves. Since there were
  scales, the harsher the handle.” 3            frequently two fibers in each micro-
                                                graph, and occasionally three, we ana-
  This opinion is further enhanced by           lyzed nearly 200 suri and huacaya
J.E. Watts and Janie Hicks:                     fibers for roundness. The elliptical sam-
  “Fine cylindrical fibers have low scale       ples tended to come from suris that
  height. When these fibers are also long,      were well locked, but not exclusively
  the fiber scales will be long as well as      so. More research will be needed to
  flat. The combination of long flat scales     determine what, if anything, this differ-
  imparts a smooth or silky feel to the         ence in fiber profile means. TEM
  wool.” 5                                      and/or skin biopsies would be a more
                                                accurate way to determine if a hair fol-
  Because wool has such a high scale            licle is round or elliptical.
height compared to suri, whether an                To an end-user, the very low scale
individual fiber is round or elliptical is      height of suri is probably of much
                                                                                                   Elliptical Suri Fiber – MSF 5.25. About 30% of
probably not as important to alpaca             more significance than if an individual
                                                                                                   the alpaca samples had one or two grooves
breeders as it is to merino producers.          fiber is round or elliptical. You can              running along the surface of the fiber, indicating
                                                appreciate this for yourself by feeling            they are probably elliptical rather than round.
Round or Ellipical Fibers?                      the “hand” of a neck scarf made from
While Watts’ and Hicks’ SRS system is           pashmina (cashmere) or merino wool
not universally accepted, their research        of similar AFD.
strongly suggests a correlation between
handle and a round rather than ellipti-         Transmission Electron
cal fiber. When I showed these SEM              Microscopy (TEM)
micrographs to Ian Watt, he soon                TEM can also be used to visualize the
found fibers that he believed were ellip-       cortex of a fiber sample. The percent-
tical rather than round. These are easily       age of orthocortical and paracortical
identified by two thin grooves running          cells in the cortex is important. Wool is
parallel to the direction of the fiber          bilateral, with both orthocortical and
with a rounded segment between them.            paracortical cells, which is responsible
   About a third of suri and huacaya            for its crimp.9 Cashmere with more
samples had one or two grooves run-             mesocortical cells, has a higher
ning the entire length of the fiber.            microfibril packing density than wool
Most were completely round. No                  of the same diameter, so it has less
llama, guanaco or camel samples                 curvature. Optical analysis of suri fiber

                                                                                                                              Spring 2006 ■ 163
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
suggests it is not bilateral, and this
                                                                                                         would help explain why it is such a
                                                                                                         straight fiber. It is much more time
                                                                                                         consuming to prepare samples for TEM
                                                                                                         than SEM, and therefore more expen-
                                                                                                         sive. TEM will probably require indus-
                                                                                                         try support from ARF, AOBA or TSN.

                                                                                                         Statistical Analysis
                                                                                                         “7.0 Scale/100 micron seems to be a
                                                                                                         distinctive parameter for suri.” 6
                                                                                                            Dr. Davitt supplied the author with
                                                                                                         1.7 megabyte, 8 bit gray scale, TIFF
                                                                                                         files captured with a Scion Grabber
                                                                                                         card from the SEM. Two micrographs
                                                                                                         were taken of each sample. One to
MSF 5.O Suri is nearly as bright as mohair but    MSF 5.75. Suri breeders need to reduce the AFD         three fibers could be measured in each
has a much lower AFD.                             of their clip to be competitive with other specialty   micrograph. These black and white
                                                  fibers. This sample is both fine (
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
Suri alpacas do have guard hair. Here we see a      The number of scales per 100µ varied depend-     Bright Huacaya – MSF 11. Huacaya fiber had a
24µ secondary fiber in front of a 40µ guard hair.   ing on where you counted them. Suri guard hair   higher MSF than suri and a 70 degree rather
                                                    had a MSF of 8.65 with a range of 4-13.          than 45 degree scale edge angle.

■   MSF 5.5 2nd & 3rd fleece                        to identify. There was a significant dif-
■   MSF 5.8 virgin fleece                           ference between the MSF of guard hair
■   Range 3-10.25                                   on suris between Phase 1 & 2. This
■   MSF guard hair 8.65                             was probably due to the uniform col-
■   Range 4-12                                      lection site used in Phase 2 and the fact
■   Phase 1 & 2 combined MSF 6.15                   we intentionally included some samples
■   Substantially lower than Antonini’s             that were “hairy” or had a stronger
    Peruvian data (7.5).                            AFD in Phase 1. It is probably accurate
                                                    to say that SAC typically have guard
   MSF should not be disassociated                  hair with about 16 scales/100µ.
with scale height. When the scale
height is impossible to measure, and                Huacaya
you have to zoom in on the micro-                   Eight huacayas were evaluated, five
graph to 200% actual size to even visu-             males and three females. Samples were
alize a scale edge, you have what is                collected from virgin fleece. These were
essentially a mono-filament like silk.              highly-evolved huacayas, with 2.0 to
                                                                                                     Fine Huacaya – MSF 11.0. Huacaya samples
Several of the suris with a relatively              3.0 crimp per cm, clearly defined
                                                                                                     were very fine, seldom exceeding 19µ.
high MSF of 7-8 were so smooth that                 bundling, and two were unusually
it still had a very slick, cool handle.             bright. AFD was 16-19 micron. While
                                                    our suri samples were randomly select-
Guard Hair                                          ed, based on fleeces or Yocum-McColl
Primary hair follicles (guard hair) of              samples we had on hand, these hua-
the Camel Family had a distinctly dif-              cayas were hand-picked to represent a
ferent scale pattern than the secondary             dense, bundled, extremely fine, or very
fibers. With the exception of vicuñas,              bright fleece. Huacaya samples had an
they were usually much stronger (high-              MSF of 11.0, which is similar to wool,
er AFD) and had an extremely high                   with a range of 8.0-12.0 MSF. This
frequency of scale. These primary fibers            correlates to an average scale length of
had extremely low scale height, and                 9.0µ. Scale height was greater than suri,
despite their AFD and scale frequency               but typically
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
ples, the longest scale length also had      to a single-coat fleece of this quality.
                                                    the greatest amplitude of crimp. This        These llamas had a scale height less
                                                    was a bright, well-bundled, “elite”          than or equal to huacaya alpacas.
                                                    fleece. Primary and secondary fibers         Handle could be either warm or cool.
                                                    were nearly the same AFD, though the           The suri-llama samples had an MSF
                                                    primary fibers could always be identi-       of 7.0, which is slightly superior to
                                                    fied by their high frequency, and            Antonini’s Peruvian suri alpacas. Six of
                                                    “sheepy” scale pattern.                      the 11 donors were top ten World
                                                                                                 Futurity suri-llama champions. Alpaca
                                                                                                 breeders should take note of how
                                                                                                 quickly llama breeders have been able
                                                                                                 to improve fiber characteristics.
                                                                                                 Huacaya breeders should be able to add
                                                                                                 brightness to their fleeces relatively
                                                                                                 quickly using SEM, and suri breeders
Guard hair from a short-wool, double-coat llama                                                  should be able to simultaneously
had a MSF of 16.5 with a range of 14-19. This                                                    reduce AFD while increasing luster.
sample is 50 micron AFD, which is not unusual.
                                                    Llama                                        Baby Camel
                                                    There are three distinct breed-types of      The two Baby Camel samples (Bactrian
                                                    llama raised in the United States. We        camel) were imported from China.
                                                    tested examples of each, including: a        They had been de-haired to less than
                                                    traditional short-wooled, double-coated      2% coarse fibers (>30µ). The Baby
                                                    ccarra llama (32µ AFD and >50%               Camel was very fine,
Surface Scanning Electron Microscopy of Suri Alpaca Fiber and Other Members of the Camel Family
■   De-haired baby camel                    finer secondary fibers. It would be
■   Low MSF (6.875)                         interesting to analyze de-haired
■   Higher scale height than suri           guanaco roving.
■   Crimp reduces luster & handle
■   Brighter if straightened

  Suri breeders should be proud of the
fact that a random sampling of Bolivian
suri alpacas equals or exceeds the MSF
of washed and de-haired Baby Camel.

                                                                                     Baby Camel – MSF 6.875. Crimp greatly reduced
                                                                                     the luster of this de-haired roving. Straightening
                                                                                     the fibers showed its inherent luster.

Guanaco
Dr. William Franklin, one of the            Vicuña
world’s leading authorities on guanacos     We analyzed three wild and three
and vicuñas, supplied wild-caught gua-      domestic vicuñas. Dr. Franklin sup-
naco samples from the Falkland              plied the vicuña samples from the
(Malvinas) Islands. Four of the samples     Pampa Galeras National Vicuña
came from the back near the top line,       Reserve in Peru, and Dr. Toni Cotton
and one from two males who were bit-        the domestic vicuñas from Jack and
ing each others’ necks. Four were red-      Miriam Donaldson’s farm in Findlay,
dish-brown to fawn in color and one         Ohio. The wild vicuñas were two
sample was white, probably coming           adults and one cria. Samples were col-
from high on the side. This was truly a     lected from center/middle back, top
random sampling!                            center neck, and center of back with
■ 5 samples from the Falkland Islands       guard hair intact. The domestic sam-
■ MSF 11.0                                  ples were from adult males, and had      Guanaco fiber was characterized by 60 micron
                                                                                     guard hair and very fine undercoat. The guard
■ 10-60 micron AFD                          been de-haired by hand. Staple length
                                                                                     hair is in front of the 10-12µ undercoat .
■ Lower MSF than vicuña                     was 40-50mm. The providence of cap-
■ Distinct guard hair with undercoat        tive vicuñas is not as likely to be as
                                            pure as wild vicuñas, especially in
   Based on the similarity of their inci-   European zoos where South American
sors, the guanaco is probably closely       exhibits will frequently house all the
related to the llama, whereas the vicuña    members of the SAC together.
is closely related to the alpaca. The       However, these samples had largely
MSF of these authentically-pure guana-      similar MSF and AFD.
cos certainly supports a close relation-    ■ 6 samples (3 wild-caught)
ship between the guanaco and the dou-       ■ Range 8.5-15 scales/100 microns
ble-coat ccarra-type llama. The under-      ■ MSF 12.1875
coat of the guanaco samples was very        ■ 12-15µ AFD
fine, and some fibers were as small as      ■ Very uniform
10 microns! However, the guard hair
was much more prevalent than any of           The vicuña samples were among the
the other breeds or species of SAC, and     most interesting of the study. They
several 60-micron guard hairs would         had a higher MSF than either hua-
                                                                                     Vicuña – MSF 12.875. The vicuña samples had a
dominate nearly every micrograph,           cayas or llamas, and the difference      higher MSF than llama, guanaco or huacaya.
making it difficult to isolate the much-    between primary and secondary fibers     AFD 12-15µ.

                                                                                                               Spring 2006 ■ 167
was difficult or even impossible to          MSF is intermediate between a huacaya
                                                  determine by SEM. This may be one            and suri, which may or may not be due
                                                  reason the MSF was higher, since with        to their huacaya ancestry. (The suri
                                                  all other members of the SAC, it was         with lowest MSF had the greatest per-
                                                  possible to count primary and second-        centage of huacaya pedigree). The scale
                                                  ary fibers separately. This will be          edge and height on 2 of the samples
                                                  amusing to those of you who have             was more distinct than most suris, par-
                                                  ever de-haired a vicuña fleece. (Those       ticularly one of the blacks.
                                                  little red guard hairs are easy to see but
                                                  hard to pluck out!) The MSF and              Paco-Vicuña
                                                  AFD of the vicuña samples were the           A paco-vicuña is the offspring of an
                                                  most uniform of all SAC tested. This         alpaca and a vicuña. A single paco-
                                                  is a significant observation, consider-      vicuña sample was evaluated from the
                                                  ing the random nature of the wild-           herd of Phil and Chris Switzer in Estes
                                                  caught samples. A follow-on analysis         Park, Colorado. Five fibers were ana-
Suri x Huacaya Cross – Average MSF 8.4. Three     of vicuña fiber with SEM should              lyzed in two micrographs. The provi-
F1 or BC1 suri x huacaya crosses from Australia   probably analyze guard hair separately       dence of this animal could not be veri-
were analyzed.                                    from the under-coat.                         fied, but this breeding male had many
                                                                                               vicuña-like characteristics, including
                                                  HYBRIDS:                                     body size, weight, bi-coloration, staple
                                                  First-Generation Suri x                      length, and personality. It was an in-
                                                  Huacaya Cross                                utero offspring out of an imported
                                                  Kenneth Madl of Aviana Farms, sup-           Chilean huacaya.
                                                  plied three colored suris from his herd      ■ One sample
                                                  which is agisted in Strathbogie,             ■ Range 11-17 scales/100 microns
                                                  Victoria, Australia. Two were black and      ■ 13.6 MSF
                                                  one silver-grey, 11 months old. Two          ■ 13-24 micron AFD
                                                  were F1 suri’s with 2-3 generations of       ■ Higher MSF than huacaya or vicuña
                                                  suri x huacaya crosses in their pedigree,
                                                  and one was a Back Cross 1 with two            This animal had the highest MSF of
                                                  generations s x h and one s x s. Two of      the series, which probably goes a long
                                                  these went on to win their classes at the    way toward proving its vicuña pedigree.
                                                  2005 Australian national alpaca show.        The uniformity of AFD was not nearly
                                                  Over 3,200 suri alpacas were imported        as pronounced as a pure vicuña.
Paco-Vicuña – MSF 13.6. Paco-vicuñas had the      into the United States from Peru,
highest MSF in the study, and a much greater
                                                  Bolivia, and Chile between 1991-98.          OTHER SPECIALTY FIBERS:
range of AFD than the pure-bred vicuñas.
                                                  With such a large population to start        Soft Rolling Skin (SRS) Merino
                                                  with, the vast majority of American          As anyone who has ever shopped for an
                                                  breeders do not cross huacaya and suri       expensive man’s suit can tell you, ultra-
                                                  alpacas. Many fewer suris were import-       fine merino wool should probably be
                                                  ed into Australia, and as a result it is a   classified as a “specialty” fiber. Even an
                                                  commonly-accepted practice in that           untrained hand can distinguish the dif-
                                                  country to cover a huacaya female with       ference between a 16µ wool and more
                                                  a suri male.                                 common fabric. When merino is
                                                  ■ Three samples from Australia               blended with cashmere or suri, the
                                                  ■ Two true black, one silver-gray            handle improves even more. The two
                                                  ■ Range 6.25-11 scales/100µ                  SRS merino samples we tested had a
                                                  ■ 8.4 MSF (intermediate between              MSF of 8.6, which is exceptional for
                                                     huacaya & suri)                           wool. These two- and three-year-old
                                                                                               merino rams had a scale height of just
                                                    As the photos indicate, these alpacas      3-4 microns, half that of most sheep
                                                  are phenotypically suri and would not        breeds. However, the abrupt scale edge
SRS Merino – MSF 8.6. While half the scale
                                                  be out of place in most colored suri         was easily measured. These were
height of other sheep breeds, SRS merino is
still more than ten times greater than huacaya    alpaca herds in the United States.           remarkably well-bundled fleeces, with
or suri alpaca.                                   While it is a small sample size, the         each lock measuring less than ½ cm

168 ■ Alpacas Magazine
with SEM, the alpaca industry should
                                            be able to substitute suri fiber for many
                                            applications that currently use cash-
                                            mere or mohair.

SRS Merino Fleece

                                            Mohair Fleece
with 6-7 crimps per cm. (This is two                                                    White Chinese cashmere, MSF 8.625, 14.2µ AFD.
to three times the curvature of a typical   Mohair
huacaya). The two samples were pro-         We evaluated a single sample of
vided by Ian Watt of Moro Bay,              imported fawn mohair. Most mohair
California.                                 production comes from South Africa.
                                            Mohair is brighter than cashmere, and
Cashmere                                    subjectively, appears to be as bright as
SEM has demonstrated that suri fiber        suri. This sample had been washed.
is most similar to cashmere and             Even though mohair is very coarse
mohair. These goat fleeces must be de-      (typically
■   Single coat fleece                               from the silk worm’s cocoon, and again
                                                    ■   11-15 micron AFD                                 after it is wound. It was very bright.
                                                    ■   Spiral scale pattern                             Even at 1,000 X, it was not possible to
                                                    ■   Range 10-12 scale/100 microns                    see where the filaments had been reeled
                                                    ■   10.6 MSF                                         together. It appears that silk is the only
                                                                                                         natural fiber which has a smoother
                                                       Angora is so fine and without guard               surface structure than suri.
                                                    hair that we could easily measure three
                                                    fibers per micrograph. The deep                      Challenges and
                                                    grooves between the spiral-shaped                    Recommendations
                                                    scales probably reduce the handle of                 SEM of suri fiber is a fortuitous con-
                                                    Angora in the same way that an abrupt                vergence of research and marketing.
                                                    scale edge effects wool. The scale edge                 Suri alpacas have a lower MSF and
                                                    angle is similar or greater than huacaya.            much lower scale height than cashmere,
                                                                                                         but no one knows it! To secure their
Bombay Silk – AFD 10-12µ. Silk does not have        Bombay Silk                                          place as the natural fiber with the
a cuticle scale since it is reeled together from    Imported Bombay silk had an AFD of                   greatest combination of fineness and
4-20 filament ends from the silk worm.              10-12 micron. “Bombay” silk comes                    luster, AOBA judging standards need
                                                    from the mulberry leaf eating silk                   to prize luster as highly as an independ-
                                                    worm. One-hundred-ten-thousand                       ent lock structure. And we believe this
                                                    tons of silk are sold each year, making              needs to occur in the show ring, not
                                                    it the dominant luxury fabric in the                 just on the score card for a fleece com-
                                                    world. Since it is not a mammal, silk                petition. Additionally, we recommend
                                                    does not have a cuticle scale structure.             that the Alpaca Research Foundation
                                                    Silk is reeled together from 4-20 indi-              (ARF) help sponsor research on suri
                                                    vidual filament ends to make a single                fiber. (The Suri Network already is).
                                                    monofilament that adheres together                   Clearly, AOBA and the Alpaca Fiber
                                                    due to the gummy texture on the sur-                 Cooperative of North America
                                                    face of the filament. Silk is washed in              (AFCNA) both need to make suris’
                                                    warm water to separate the filament                  unique characteristics an integral part

IWTO methodology views fiber samples at 600 X
magnification which allows 5-6 fibers to be              Acknowledgements
analyzed at a time. Dr. Davitt increased magnifi-
cation to 1,000 X so suri scales could be visual-        The authors would like to thank the following friends and colleagues for their assistance:
ized more easily.                                        ■ Dr. William Franklin, who supplied us with wild-caught guanaco and vicuña samples
                                                           from Pampa Galeras and the Falkland Islands.
                                                         ■ Dr. Toni Cotton of Findlay, Ohio, who provided the domestic vicuña samples.
                                                         ■ April and Richard Angotti of Benvenuti Farms in Bend, Oregon, who
                                                           provided the highly-evolved huacaya samples.
                                                         ■ Sherry Sheridan of Aloha Farms in Bend, Oregon, who provided the
                                                           double-coat llama sample.
                                                         ■ Susan Tellez for her foreign language literature search of SEM of the SAC.
                                                         ■ Kenneth Madl, who provided us with many publications on contemporary wool and
                                                           cashmere research from Australia, and his first-generation suris from suri x huacaya
                                                           crosses.
                                                         ■ Ian Watt, for help in identifying round versus elliptical fibers, and for providing the
                                                           highly-evolved SRS merino samples.
                                                         ■ Our local wool guild, the Textilians, who provided us with cashmere, silk, and Baby
                                                           Camel (Bactrian camel) samples.
                                                         ■ Angus McColl, who provided the white Chinese cashmere.
                                                         ■ Dr. Kathleen Cullen of Spokane, Washington, who provided the white Angora.
                                                         ■ Chris and Phil Switzer of Estes Park, Colorado who supplied the paco-vicuña sample.
MSF was not related to AFD as it appears to be
                                                         ■ Dr. Chris Davitt, WADDL, who prepared and scanned the fiber samples and tutored
with other specialty fibers. This is good news
since it suggests you can select for enhanced              me on the use of imaging software.
luster and reduced AFD simultaneously.

170 ■ Alpacas Magazine
of their marketing and advertising cam-    ■   The “long smooth scale”™ of suri
paigns. The low scale height of huacaya        fiber averages 16.3µ long
fiber compared to wool can also be         ■   Huacaya samples averaged 9.0µ
emphasized! To be competitive in the       ■   This equates to a MSF of 6.125/
luxury fiber market, suri breeders need        100µ suri and 11.0 MSF/100µ
to reduce the AFD of their clip.               huacaya
Fortunately, there was no correlation      ■   MSF was not correlated to fiber
between luster and AFD in either this          diameter.
or Antonini’s study, and it should be      ■   A low MSF was not correlated to a
possible to select for both characteris-       twisted lock and may be inversely
tics simultaneously.                           related
   Cheryl and I have been raising alter-   ■   Scale height on both huacaya and
native livestock for over thirty years.        suri is much less than previously
                                                                                                    It would be premature to assign value to one
As a result we usually take a macro            reported.
                                                                                                    lock type over another until a more thorough
view of the issues which face our                                                                   study of SEM is made of suri alpaca fiber.
industry. We have learned that if you
do not breed for something, you lose       Andy and Dr. Cheryl Tillman funded this
it. Whether this is maternal ability       research on suri alpaca fiber. The Tillmans
(milk production), thriftiness, confor-    imported the influential colored suri alpacas
mation, or luster, if you take a charac-   from Hacienda Acero Marka near La Paz,
teristic for granted, you will lose it.    Bolivia in 1996. Andy has raised llamas since
The results of this research are both      1975 and alpacas since 1982. He is a member
exciting and sobering. For instance,       of The Suri Network research committee.
there is certainly no reason not to        Cheryl Tillman has been a camelid veterinarian
show shorn suris in AOBA halter            since 1985. She sits on the board of directors
classes, due to the close correlation      for the US Animal Health Association, serves
between a virgin, 2nd, and 3rd fleeces     on the AOBA/ARI Government & Industry
MSF. On the other hand, we are             Relationship committee, and has reviewed
probably not using some of our best        research proposals for Morris Animal
males, those with the greatest luster      Foundation for many years. They can be
and lowest MSF.                            contacted at andy@tillmansranch.com.

Summary                                                                                             A fan-shaped lock and narrow flat lock may be
It would be premature to assign value                                                               under-appreciated for luster.
to one lock type over another until a
more thorough SEM study is made of         REFERENCES
suri alpaca fiber. Like many scientific        1. College Physics, 4th edition, Franklin Miller Jr., pg. 659
enquiries, this one has raised as many         2. Purely Suri magazine, volume 2, 2004, Suvia Judd and Deborah Berman, pg 18
questions as it has answered. The              3. “Is Merino Wool a Luxury Precious Fiber?,” Bruce McGregor, Australian Farm Journal,
authors have scanned enough fiber                 July, 2003
samples to determine that suri alpaca          4. “Influence of Nutrition, Fiber Diameter, and Fiber Length on the Fiber Curvature of
fiber is measurably different in scale            Cashmere,” B.A. McGregor, The Australian Journal of Agricultural Research, 2003
length, frequency, edge height, and            5. J.E. Watts and Janie Hicks: The Soft Rolling Skin® (SRS)® Breeding System for Alpacas.
edge angle from wool and huacaya               6. “SUPREME-Project: Cuticular Cell Mean Scale Frequency in Different Types of Domestic
fiber. It is probably most similar to             South American Camelids (SAC)”; M. Antonini, E. Frank, M. Gonzales, F. Pierdominici,
cashmere and mohair but with an even              S. Catalano, M.V.H. Hick, & F. Castrignano; European Symposium on SAC, University
lower scale height. The “take home”               di Camerino Press, Matelica (MC) Italy, pp. 142-152 (1996)
lesson from this SEM study is that             7. IWTO DTM-XX-97: “Quantitative Analysis of Blend of Wool with Specialty Fibers by Scanning
what is truly unique about suri alpaca            Electron Microscopy,” International Wool Textile Organization (1997)
fiber is its luster. This should be kept       8. “Characterization of Specialty Fibers by Scanning Electron Microscopy”; Phan K.H.,
foremost in mind in any future discus-            Wortmann F.J., Wortmann G., and Arns W.; Shriftenz. Dtsch. ; Wollforxchungsinst;
sion of possible breed standards, end-            p. 106 (1988)
use, or show standards.                        9. Properties and Performance of Goat Fibre, JD Leeder, BA McGregor and RG Steadman (1998)
■ Suri and huacaya fleece can be differ-   10. Elite Natural Fibre, Grower Expectations & Processor Requirements, Peter J. Sporle, 1994
   entiated by SEM                         11. Alpaca: Large Scale Industrial Perspective on Fiber Processing, Juan Pepper, 2005

                                                                                                                             Spring 2006 ■ 171
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