Bh 2020 - 2021 What's New ? - High Performance Photon Counting - Becker & Hickl GmbH
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bh 2020 - 2021 What's New ?
High Performance
Photon Counting
bh 2020 - 2021 What's New ?
DCS-120 Confocal and Multiphoton FLIM Systems: New Generation DCS Black
The new generation of the bh DCS-120 FLIM system features unprecedented temporal resolution, unprecedented timing
reproducibility, high spatial resolution, high sensitivity, and near-ideal photon efficiency. Fluorescence lifetimes can be
detected down to 10 ps. The decay data can be resolved into 4096 time channels, with a minimum time bin width of 405 fs.
Pixel numbers as high as 4096 x 4096 are available. The system is available in a confocal version with excitation by ps diode
lasers, or as a two-photon version with a Ti:Sa laser or a femtosecond fibre laser.
The DCS-120 FLIM system uses fast scanning by galvanometer mirrors, confocal or non-descanned detection, and FLIM by
bh’s multidimensional TCSPC technique. Data acquisition functions include precision dual-channel FLIM, laser wavelength
multiplexing, multi-wavelength FLIM, time-series FLIM, ultra-fast time-series recording by temporal-mosaic FLIM, spatial
mosaic FLIM, Z stack FLIM, phosphorescence lifetime imaging (PLIM), and fluorescence lifetime-transient scanning
(FLITS).
Data analysis is performed by next generation SPCImage NG. Lifetime analysis is running on a GPU, reducing calculation
time from previously several minutes to a few seconds. Other features are image segmentation via a phasor plot or via 2D
time-domain histograms, and automatic modelling of the system IRF. Together with the extraordinary timing stability of the
recording system, repeated recalibration of the IRF is no longer necessary.
Target applications of the new DCS-120 system are molecular imaging in live cells and tissues, protein-interaction
experiments by FRET, metabolic imaging by NADH / FAD decay parameters, identification of cancer cells, and the recording
of dynamic physiological processes of cell metabolism and neurology.
28 Years Technology Leader in TCSPC. More than 2500 TCSPC systems worldwide.
2 bh-whats-new-2021-02 June 2021
bh 2020 - 2021 What's New ?
FLIM Systems for Zeiss LSM 980 Laser Scanning Microscopes
Confocal or Multiphoton Systems
Detectors for Confocal BIG Ports or Multiphoton NDD ports
High Efficiency GaAsP or Ultra-Fast Hybrid Detectors
IRF width down to 19 ps fwhm
Up to 4 Picosecond Diode Lasers
Single or Multiplexed Laser Operation
Single or Multiplexed FLIM Recording
Simultaneous FLIM/PLIM
Spatial and Temporal Mosaic FLIM
Recording of Dynamic Effects Down to the Millisecond Range
2, 3, or 4 Parallel Detection channels
Data Analysis by SPCImage NG
Ultra-Fast Data Analysis by GPU Processing
bh 'Laser Hub' Four-Laser Excitation Module Detector Assembly IRF, Multiphoton system with Ultra-Fast Detector
SPCM Data Acquisition Software, with four laser channels SPCImage NG Data Analysis Software
Please see:
1. Becker & Hickl GmbH, Modular FLIM systems for Zeiss LSM 710 / 780 / 880 family laser scanning microscopes. User
handbook, 7th ed. (2017). Available on www.becker-hickl.com
2. FLIM Systems for Zeiss LSM 980 Laser Scanning Microscopes (2020). Available on www.becker-hickl.com
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 3
bh 2020 - 2021 What's New ?
Nikon A1 Laser Scanning Microscopes Integrate bh FLIM
FLIM by bh's Multi-Dimensional TCSPC Technique
Integrated in Nikon ‘Elements’ Microscope Software
Excellent Time Resolution, Excellent Spatial Resolution
Megapixel Images
Online-Lifetime Display Function
Precision Data Analysis by bh SPCImage
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
4 bh-whats-new-2021-02 June 2021
bh 2020 - 2021 What's New ?
DCS-120 MACRO System Runs Fast-Acquisition FLIM
The bh DCS-120 MACRO scanner can now be combined with bh's FASTAC fast-acquisition FLIM system. The system
employs confocal scanning of an image area as large as 15 x 15 mm, and FLIM recording in four parallel SPC-150N TCSPC
modules. Image formats are from 64 x 64 pixels up to 2048 x 2048 pixels, with up to 1024 time channels. Images of 256 x 256
pixels can be recorded within less than 0.5 seconds, images of 512 x 512 pixels within less than 2 seconds. Time-channel
width can be selected shorter than 1 ps, the IRF width with fast HPM-100-06 and -07 hybrid detectors is less than 25 ps full
width at half maximum.
Fig. 1: Left: DCS-120 MACRO with bh FASTAC FLIM system. Right: FLIM image with 512 x 512 pixels, 1024 time channels, recorded
in 2 seconds.
DCS-120 MACRO FLIM System Detects Tumors in Mice
The bh DCS-120 MACRO detects tumors in mice via FLIM of endogenous NAD(P)H. The imaging system uses confocal
scanning in combination with ps-diode laser excitation and bh's multi-dimensional TCSPC process. For NAD(P)H FLIM, a
375-nm picosecond diode laser is used for excitation, emission is detected through a 440 to 475 nm bandpass filter. Tumor
detection exploits the fact that the free-to-bound ratio of NAD(P)H changes with the metabolic state. Since bound and free
NAD(P)H have different fluorescence lifetimes a shift in the metabolic state results in a change in the decay profiles.
Fig. 2: Left: tm image of a tumor in a mouse, recorded by DCS-120 MACRO. Right: Decay functions in tumorous area (red)
and in healthy area (blue)
Please see: V. I. Shcheslavskiy, M. V. Shirmanova, V. V. Dudenkova, K. A. Lukyanov, A. I. Gavrina, A. V. Shumilova, E. Zagaynova, W.
Becker, Fluorescence time-resolved macroimaging. Opt. Lett. 43, No. 13, 3152-5155 (2018)
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 5
bh 2020 - 2021 What's New ?
New SPCImage NG Data Analysis Runs GPU Processing
SPCImage NG is a new generation of bh's TCSPC-FLIM data analysis software. It combines time-domain and frequency-
domain analysis, uses a maximum-likelihood (MLE) algorithm to calculate the parameters of the decay functions in the
individual pixels, and accelerates the analysis procedure by GPU processing. 1D and 2D parameter histograms are available to
display the distribution of the decay parameters over the pixels of the image or over selectable ROIs. Image segmentation can
be performed via the phasor plot, and pixels with similar decay signature can be combined for high-accuracy time-domain
analysis. SPCImage NG provides decay models with one, two, or three exponential components, incomplete-decay models,
and shifted-component models. Another important feature is advanced IRF modelling, making it unnecessary to record IRFs
for the individual FLIM data sets.
For details please see
1. SPCImage NG overview brochure, available on www.becker-hiockl.com
2. Chapter SPCImage NG data analysis software. In: The bh TCSPC Handbook, 8th edition (2019)
Fig. 3: SPCImage NG Main panel. FLIM image (left) phasor plot (top right) and decay curve in selected pixel (bottom right)
Fig. 4: Cell with interacting proteins, labelled with a FRET donor and a FRET acceptor. Left to right: Classic FRET efficiency,
FRET efficiency of interacting donor fraction, FRET distance
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
6 bh-whats-new-2021-02 June 2021
bh 2020 - 2021 What's New ?
New IRF Model Makes IRF Recording Unnecessary
SPCImage NG data analysis software models the detector IRF by a function of the type xꞏe-x. This function closely resembles
the IRF of hybrid detectors with GaAsP cathodes. It also fits reasonably well to the response of other detectors. SPCImage
synthesises a system IRF by convoluting the function xꞏe-x with a gaussian laser pulse of predefined width. It then
automatically adjusts the parameter x to obtain an optimal fit of the combined decay data in a region of interest. The IRF
obtained this way is used for analysis of the entire lifetime image. Two examples for automatically modelled IRFs are shown
in Fig. 5. The fits obtained with the synthetic IRF are so good (see Fig. 5) that IRF recording for FLIM analysis is no longer
necessary.
Fig. 5: IRF of the type xe-x. Left: Modelled for ps diode laser plus HPM-100-06 hybrid detector. Right: IRF modelled for
HPM-100-40 hybrid detector and ps diode laser.
Mosaic FLIM Records Precision Lifetime Data from a Moving Object
The bh TCSPC FLIM systems are able to record fluorescence lifetime images from a moving object. The technique is based on
temporal-mosaic recording and image segmentation by the phasor plot of bh SPCImage NG data analysis software. A cluster
of phasors is selected in the phasor space, identifying pixels of a given decay signature in the FLIM mosaic (Fig. 6, left).
These pixels are back-annotated in the mosaic, selecting details of the object irrespectively of their location in the individual
images. The decay data of the pixels within the selected areas are summed up (Fig. 6, right). The result is a single decay curve
of the detail of interest with extremely high photon number. This curve is then analysed at high precision.
For details please see:
Precision Fluorescence-Lifetime Imaging of a Moving Object. Application note, available on www.becker.hickl.com
Fig. 6: Autofluorescence mosaic data of a leg of a water flee. Frame time 0.5 seconds, 64 frames recorded in FLIM mosaic.
Left: Data loaded into SPCImage, phasor plot activated. Note the low photon number in decay curve of a single pixel. Right:
Features with yellow phasor signature selected by 'Select Cluster' function of phasor plot, decay curves of selected pixels
summed up by 'Sum up decay curve' function. Resulting decay curve shown lower right. The resulting curve is analysed at
high precision, decay parameters shown upper right.
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 7
bh 2020 - 2021 What's New ?
Shifted-Component Model Solves Long Problem of FLIO Data Analysis
bh developed a new model for analysis of fluorescence-lifetime ophthalmoscopy data recorded with the Heidelberg
Engineering FLIO system. The model uses three exponential components, two of which describe the fundus fluorescence,
whereas the third one models the fluorescence of the crystalline lens. The third component is shifted toward short times,
accounting for the difference in signal transit time. Compared with the standard triple-exponential model, the fit stability and
the lifetime reproducibility are massively improved. Most importantly, the new model is able to extract correct fundus
lifetimes in the presence of strong fluorescence from the crystalline lens. The new model is especially beneficial for
diagnosing cataract patients for which correct fundus FLIO data are difficult to obtain. It runs under SPCImage NG, bh’s new-
generation FLIM data analysis software. By using MLE fitting and GPU processing, the new FLIO analysis is more than 20
times faster than previously used algorithms.
For details please see
1. The bh TCSPC Handbook, chapter Ophthalmic FLIM, page 524.
2. W. Becker, A. Bergmann, L. Sauer, Shifted-component model improves FLIO data analysis. Application note,
available on www.becker-hickl.com
4. Wolfgang Becker, Cornelia Junghans, FLIO, The Road to Success. Education and training material. Available on
https://www.becker-hickl.com/literature/application-notes
3. Fluorescence-Lifetime Imaging Ophthalmoscopy. Principles, Challenges, Solutions, and Applications. Lecture for
FLIO users. Available on https://www.becker-hickl.com/literature/application-notes. For a video of the lecture,
please contact bh.
Fig. 7: Fundus FLIO image of the eye of a cataract patient. Left: Amplitude-weighted lifetime, tm, of triple-exponential model.
Due to strong lens fluorescence tm is entirely off range. Middle: Amplitude-weighted lifetime, tm12, of component 1 and 2 of
the shifted-component model. The tm12 image shows the correct lifetime of the fundus. Right: Image taken after the patient
obtained a cataract surgery. The lifetimes are identical with that of the pre-surgery tm12 image. Heidelberg-Engineering FLIO
system with bh TCSPC FLIM, bh SPCImage NG FLIM data analysis software.
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
8 bh-whats-new-2021-02 June 2021
bh 2020 - 2021 What's New ?
Metabolic Imaging with the DCS-120 Confocal FLIM System: Simultaneous FLIM of NAD(P)H
and FAD
The bh DCS-120 Confocal Scanning Metabolic FLIM System detects changes in the metabolic state of live cells.
Information on the metabolic state is derived from the fluorescence decay functions of NAD(P)H and FAD. Two ps
diode lasers, with wavelengths of 375nm and 405 nm, are multiplexed to alternatingly excite NAD(P)H and FAD.
One FLIM channel of the DCS system detects in the emission band of NAD(P)H, the other in the emission band of
FAD. The FLIM data are processed by SPCImage data analysis software. For both channels, the data analysis
delivers images of the amplitude-weighted lifetime, tm, the component lifetimes, t1 and t2, the amplitudes of the
components, a1 and a2, and the amplitude ratio, a1/a2. Moreover, it delivers the fluorescence-lifetime redox ratio
(FLIRR), a2nadh/a1fad. A shift from oxidative phosphorylation to glycolysis or back is revealed by changes in tm, a1
or a1/a2, and in the FLIRR.
Fig. 8: NAD(P)H a1 images for normal cells (left) and tumor cells (right). Lower row: Histograms of a1 over the pixels of the images. The
tumor cells have 15% higher a1.
For more information please see:
1. Becker & Hickl GmbH, Metabolic Imaging with the DCS-120 Confocal FLIM System: Simultaneous FLIM of NAD(P)H
and FAD. Application note, available on www.becker-hickl.com
2. The bh TCSPC Handbook, 8th ed. (2019)
3. R. Suarez-Ibarrola, L. Braun, P. F. Pohlmann, W. Becker, A. Bergmann, C. Gratzke, A. Miernik, K. Wilhelm, Metabolic
Imaging of Urothelial Carcinoma by Simultaneous Autofluorescence Lifetime Imaging (FLIM) of NAD(P)H and FAD.
Clinical Genitourinary Cancer (2020)
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 9
bh 2019 - 2020 What's New ?
2-Photon Excited Metabolic Imaging with the DCS-120 MP System: Simultaneous 2p FLIM of
NAD(P)H and FAD
The DCS-120 2p metabolic FLIM system is based on a combination of the bh DCS-120 MP multiphoton FLIM systems with
the 'FemtoFibre Dichro', femtosecond fibre laser of Toptica GmbH (Munich, Germany). The laser emits at 780 nm and at
880 nm. It contains individual AOMs for the two wavelengths. The AOMs are controlled by digital on/off (beam blanking)
signals and by analog intensity signals. The laser can be directly controlled by the outputs of the bh GVD-120 scan controller.
It is thus a perfect match to the bh DCS-120 MP multiphoton FLIM system. The combination of both makes an almost ideal
two-photon metabolic FLIM system. A photo of the system is shown in Fig. 9, left. The control of the laser is fully integrated
in the SPCM FLIM data acquisition software via the scan control panel, see Fig. 9, left. The two laser wavelengths, 780 nm
and 880 nm, can be turned on or off independently by the blue buttons in the upper right of the panel. The intensities are
controlled via the Power sliders. The wavelengths are multiplexed by the multiplexing function of the GVD-120 scan
controller. With the settings shown in Fig. 9 both wavelengths are active, and multiplexed frame by frame.
Fig. 9: DCS-120MP Metabolic FLIM System with Toptica FemtoFibre Dichro. Cover of beam path removed.
The system simultaneously records an NADH FLIM image, an FAD FLIM image, and an SHG image. Examples are shown in
the figures below. For more information please see ‘The bh TCSPC Handbook’, 8th ed. (2019), page 357.
Fig. 10: 2p metabolic FLIM of Pig skin, tm image in the NADH channel (top left) , the FAD channel (top right) , and the SHG
channel (bottom).
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
10 bh-whats-new-2021-02 June 2021bh 2020 - 2021 What's New ?
DCS-120 MP: Two-Photon FLIM with Toptica Femtosecond Fibre Laser
The DCS-120 MP FLIM multiphoton system is available with a Toptica Femto Fibre Pro laser. The laser delivers 100-fs
pulses at 780 nm. The system is thus ideally suited for metabolic FLIM by NAD(P)H imaging. However, the laser excites also
a large number of other fluorophores, such as flavines and carotinoids. Combined with bh's ultra-fast detectors, the system
delivers an instrument-response width of less than 20 ps. In biological systems the DCS-120 MP reveals ultra-fast decay
processes which have never been observed before.
Ultra-Fast Fluorescence Decay in Biological Objects - Detected by DCS-120 MP
2p FLIM of Mushroom Spores: t1 = 12 ps
Species: Agaricus
campestris
Spore Colour:
dark red-brown
t1: 12 ps
a1: >0.99
Zoom: 4
2p FLIM of Pollen Grains: t1 = 10 ps
Please see:
Two-Photon FLIM with a Femtosecond Fibre Laser. Application note, available on www.becker-hickl.com
Two-Photon FLIM of Mushroom Spores Reveals Ultra-Fast Decay Component. Application note, available on www.becker-hickl.com
Two-Photon FLIM of Pollen Grains Reveals Ultra-Fast Decay Component. Application note, available on www.becker-hickl.com
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 11bh 2020 - 2021 What's New ?
SPC-150NX and SPC-150NXX TCSPC Modules: Ultra-Fast Timing for Ultra-Fast
Detectors
- Ultra-fast discriminators, 5 GHz input bandwidth
- Extremely low timing jitter
- SPC-150NX: Electrical response 3.5 ps FWHM, electrical timing jitter 1.5 ps RMS
Minimum time channel width 405 fs
IRF stability better than 0.4 ps over 1 minute
- SPC-150NXX: Electrical response 3 ps FWHM, electrical timing jitter 1.2 ps RMS
Minimum time channel width 203 fs
IRF stability better than 0.4 ps over 1 minute
- System IRF widthbh 2020 - 2021 What's New ?
SPC-180NX and SPC-180NXX TCSPC Modules - Fast Timing, Fast PCIex Interface
High-throughput PCI-Express interface
Ultra-fast ultra-stable timing electronics
Electrical IRF width 3.5 ps FWHM
Internal timing jitter 1.6 ps RMS
Time-channel width down to 203 fs
Discriminator input bandwidth 4 GHz
Photon distribution and parameter-tag modes
Multi-detector / multi-wavelength capability
Excitation-wavelength multiplexing
Parallel operation of 2, 3 or 4 modules
Laser repetition rates up to 150 MHz
Dead time 80 ns
Saturated count rate 12.5 MHz
SPC-180NX: Electrical response 3.5 ps FWHM,
Electrical timing jitter 1.5 ps RMS
Minimum time channel width 405 fs
IRF stability better than 0.4 ps over 1 minute
SPC-180NXX: Electrical response 2.9 ps FWHM
Electrical timing jitter 1.2 ps RMS
Minimum time channel width 203 fs
IRF stability better than 0.4 ps over 1 minute
28 Years Technology Leader in TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 13bh 2020 - 2021 What's New ?
New Lifetime-Intensity Mode Delivers Better FLIM Images
At count rates approaching the reciprocal dead time of the TCSPC electronics the photon number in the pixels of a FLIM
image becomes a nonlinear function of the intensity, and eventually saturates. The images are therefore losing contrast in
bright regions, although the decay data in the individual pixels remain correct. To improve the image quality at high count
rates we have implemented a counter in parallel with the TCSPC timing electronics. The counter delivers pixel photon
numbers with virtually no counting loss. A new 'Lifetime/Intensity' mode of bh's SPCM software builds up FLIM images by
using pixel intensities from the parallel counter and pixel decay data from the timing electronics. These images show
substantially improved contrast at high count rates. The mode is available for the SPC-160 and the new SPC-180
TCSPC/FLIM modules. It is implemented in SPCM version 9.86 of June 8, 2021 or later.
For details please see: Lifetime-Intensity Mode Delivers Better FLIM Images. Application note, available on www.becker-hickl.com
Fig. 12: FLIM images recorded from an Invitrogen F24630 Mouse kidney section. Left: New Lifetime/Intensity mode. Right: Traditional
FLIM mode. 512 x 512 pixels, 1024 time channels, online-lifetime display function of SPCM software. Lifetime scale 2000ps to 4000ps.
Average (recorded) count rate 5.5 MHz.
SPCM Displays Decay Curves in Regions of Interest
SPCM is able to display decay curves in points or regions of interest of FLIM images. The function is available online during
the buildup of the images. To enable the function, right-click into the image window of which you want to see decay curves,
and select 'Show Decays'. For further configuration of the curve display, right-click into the curve window, and click on 'Trace
Parameters'.
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
14 bh-whats-new-2021-02 June 2021bh 2020 - 2021 What's New ?
4.4 ps FWHM IRF width with SPC-150NXX and Single Nanowire Detector: New
World Record in TCSPC Time Resolution
The SPC-150 NXX TCSPC module in combination with a superconducting single-nanowire NbN detector has
delivered an instrument response function (IRF) of 4.4 ps full width at half maximum (fwhm). We have
demonstrated the resolution of the system by recording the fluorescence decay of IR 1061, an infrared dye from
Sigma Aldrich. The fluorescence lifetime of IR 1061 is extremely short, and has never been reliable measured. The
SPC-150NX with the ultra-fast NbN detector resolves the decay curve clearly. Decay analysis with SPCImage
delivered a fluorescence lifetime of 43.7 ps.
Please see: W. Becker, J. Breffke, B. Korzh, M. Shaw, Q-Y. Zhao, K. Berggren, 4.4 ps IRF width of TCSPC with an NbN Superconducting
Nanowire Single Photon Detector. Application note, available on www.beker-hick.com
Fig. 13: Left: IRF of Detector-TCSPC combination. FHWM of IRF is 4.4 ps. Right: Fluorescence decay of IR 1061.
Fluorescence lifetime is 43.7 ps.
Sub-20ps IRF Width from HPM-100-06 and -07 Hybrid Detectors
- Unprecedented time resolution with SPC-150N and SPC-150NX TCSPC modules and fs lasers
- Excellent time resolution in fluorescence-decay and multiphoton FLIM applications
- Improved separation of components of multi-exponential decay functions
- Detection of ultra-fast decay components in biological systems
- Short distance and zero-distance NIRS and fNIRS measurements
Fig. 14: Left: IRF of HPM-100-06 (bi-alkali cathode). Right: IRF of HPM-100-07 (multi-alkali cathode). The full with at half
maximum is 18.9 ps and 19.4 ps, respectively. SPC-150NX TCSPC module, 100 ps/div, 405 fs/channel
Please see: Sub-20ps IRF Width from Hybrid Detectors and MCP-PMTs. Application note, available on www.becker-hickl.com
The bh TCSPC handbook, 8th ed. (2019), page 162
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 15bh 2020 - 2021 What's New ?
273 ps FWHM TCSPC Response with Hamamatsu H15620 NIR PMT
With the bh SPC-150NX and SPC-180NX TCSPC devices, the new Hamamatsu H15620 NIR PMT module delivers an
instrument-response width of < 280 ps FWHM. The H15620 fits smoothly into the bh TCSPC systems. The supply voltage for
the detector, the cooler current, and the gain control voltage are available from a DCC-100 detector controller module. In
combination with a HFAC-26-1 preamplifier, the DCC-100 provides also for overload shutdown. We have demonstrated the
performance of the detector at the example of diffuse optical imaging experiments at a wavelength of 1300 nm.
Please see: 273 ps FWHM TCSPC Response with Hamamatsu H15620 NIR PMT, application note, available on www.becker-hickl.com
IRF of H15620 with SPC-150 NX, 1300nm BDS-SM ps diode laser. DOT scan of the palm of a human hand. Colour represents mean time of flight
New PMCS-150-Series Detectors have 120 ps IRF Width
The PMCS-150 is a small-size (40 x 40 x 120 mm) version of the PMC-150. It was designed with special regard to the bh 12-
channel MaxTau system. The PMCS-150 has active cooling, internal high-voltage generation, and an internal preamplifier.
Different than other bh PMT modules, The PMCS-150 can be operated without being controlled from a DCC-100 detector
controller. To run independently of a DCC card, the PMCS has pre-set gain and internal overload-shutdown circuitry. When
overload occurs the detector regulates down its gain until the output current is within the safe range. When the overload is
removed the gain regulates up to the pre-set value, and the detector resumes normal operation. The PMCS-150 module is
shown in Fig. 15, left, the IRFs for illumination of a 1-mm spot and for illumination of the entire cathode are shown in Fig. 15,
right. The IRF widths are 112 ps and 127 ps, respectively.
Fig. 15: PMCS-150 detector (shown left) and IRF with SPC-150 NX (shown right)
Please see: W. Becker, The bh TCSPC handbook, 8th ed. (2019), page 157. Available on www.becker-hickl.com
28 years experience in multi-dimensional TCSPC. More than 2500 TCSPC systems worldwide.
16 bh-whats-new-2021-02 June 2021bh 2020 - 2021 What's New ? bh MaxTau 12 Channel TCSPC System The bh MaxTau system contains 12 parallel SPC-150 NX cannels in a single 19” case. The entire system is connected to a standard PC or a laptop computer via a bus extension cable. The system offers extremely high throughput rate without any crosstalk between the channels. With its
bh 2020 - 2021 What's New ?
LHB-104 'Laser Hub' Four-Laser Box
The LHB-104 ‘Laser Hub’ contains up to four bh BDS-SM picosecond diode lasers. The beams of the individual lasers are
combined into a single free-beam output or into a single-mode fibre. The box contains control electronics equivalent to the
LSB-C and LSB-C2 Laser Switch boxes. In addition, it contains wavelength-multiplexing electronics, inputs for control
signals, and outputs for the synchronisation signals to SPC modules. Software control of the LHB-104 is integrated in the bh
SPCM data acquisition Software. Please see: LHB-104 Laser Hub, User Manual. Available on www.becker-hickl.com.
Fig. 17: LHB-104 Laser Hub
Fig. 18: LHB Control panel in bh SPCM Software
LSB-C and LSB-C2 Laser Switch Boxes
LSB-C and LSB-C2 laser control boxes provide control signals to one or two BDS or BDL lasers. The devices contain an
intensity regulator, a switch to select the repetition rate, the mandatory key switch, and inputs for various external control
signals. The LSB-C and LSB-C2 switch boxes are shown in Fig. 19.
Fig. 19: LSB-C and LSB-C2 laser switch boxes for one and two BDS or BDL lasers
28 Years Technology Leader in TCSPC. More than 2500 TCSPC systems worldwide.
18 bh-whats-new-2021-02 June 2021bh 2020 - 2021 What's New ?
New and Updated TCSPC / FLIM Literature
Beautiful FLIM
The brochure presents a selection of the most beautiful images ever
recorded with bh FLIM systems. Every image comes with a
description of the technical and scientific background. For best results
print on A3 format paper, or contact bh for printed copy.
SPCImage Next Generation FLIM Data Analysis Software
20-pages overview brochure
The bh TCSPC Technique. Principles and Applications
28-pages overview brochure
Bigger and Better Photons - The Road to Great FLIM Images
39 pages education brochure
FLIM Systems for Laser Scanning Microscopes
40 pages overview brochure
The bh TCSPC Handbook
The textbook on TCSPC, FLIM, and TCSPC/FLIM applications.
954 pages, 1222 references.
Currently out in 8th edition.
New edition planned for end of 2021. Please stay tuned.
FLIM and TCSPC Posters from bh
FLIM and TCSPC posters are available from bh.
Size is A0, or 84 x 120 cm.
For complete set of posters and for ordering please see poster catalog on
www.becker-hickl.com
28 Years Technology Leader in TCSPC. More than 2500 TCSPC systems worldwide.
bh-whats-new-2021-02 June 2021 19Becker & Hickl GmbH
Nunsdorfer Ring 7-9
12277 Berlin, Germany
Tel. +49 212 800 20 Fax +49 30 212 800 213
info@becker-hickl.com https:/www.becker-hickl.com
International Sales Representatives
US: UK: Japan: China and Hongkong:
Boston Electronics Corp Photonic Solutions Tokyo Instruments Inc. DynaSense Photonics Co. Ltd.
tcspc@boselec.com sales@photonicsolutions.co.uk sales@tokyoinst.co. jp info@dyna-sense.com
www.boselec.com www.photonicsolutions.co.uk www.tokyoinst.co.jp www.dyna-sense.com
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