Funny Current Downregulation and Sinus Node Dysfunction Associated With Atrial Tachyarrhythmia

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Funny Current Downregulation and Sinus Node Dysfunction Associated With Atrial Tachyarrhythmia
Funny Current Downregulation and Sinus Node Dysfunction
        Associated With Atrial Tachyarrhythmia
                A Molecular Basis for Tachycardia-Bradycardia Syndrome
       Yung-Hsin Yeh, MD*; Brett Burstein, PhD*; Xiao Yan Qi, PhD*; Masao Sakabe, MD, PhD;
                  Denis Chartier, MSc; Philippe Comtois, PhD; Zhiguo Wang, PhD;
                               Chi-Tai Kuo, MD; Stanley Nattel, MD

Background—Sinoatrial node (SAN) dysfunction is frequently associated with atrial tachyarrhythmias (ATs). Abnormal-
  ities in SAN pacemaker function after termination of ATs can cause syncope and require pacemaker implantation, but
  underlying mechanisms remain poorly understood. This study examined the hypothesis that ATs impair SAN function
  by altering ion channel expression.
Methods and Results—SAN tissues were obtained from 28 control dogs and 31 dogs with 7-day atrial tachypacing (400
  bpm). Ionic currents were measured from single SAN cells with whole-cell patch-clamp techniques. Atrial
  tachypacing increased SAN recovery time in vivo by ⬇70% (P⬍0.01), a change which reflects impaired SAN
  function. In dogs that underwent atrial tachypacing, SAN mRNA expression (real-time reverse-transcription polymerase
  chain reaction) was reduced for hyperpolarization-activated cyclic nucleotide– gated subunits (HCN2 and HCN4) by
  ⬎50% (P⬍0.01) and for the ␤-subunit minK by ⬇42% (P⬍0.05). SAN transcript expression for the rapid
  delayed-rectifier (IKr) ␣-subunit ERG, the slow delayed-rectifier (IKs) ␣-subunit KvLQT1, the ␤-subunit MiRP1, the
  L-type (ICaL) and T-type (ICaT) Ca2⫹-current subunits Cav1.2 and Cav3.1, and the gap-junction subunit connexin 43 (were
  unaffected by atrial tachypacing. Atrial tachypacing reduced densities of the HCN-related funny current (If) and IKs by
  ⬇48% (P⬍0.001) and ⬇34% (P⬍0.01), respectively, with no change in voltage dependence or kinetics. IKr, ICaL, and
  ICaT were unaffected. SAN cells lacked Ba2⫹-sensitive inward-rectifier currents, irrespective of AT. SAN action potential
  simulations that incorporated AT-induced alterations in If accounted for slowing of periodicity, with no additional
  contribution from changes in IKs.
Conclusions—AT downregulates SAN HCN2/4 and minK subunit expression, along with the corresponding currents If and
  IKs. Tachycardia-induced remodeling of SAN ion channel expression, particularly for the “pacemaker” subunit If,
  may contribute to the clinically significant association between SAN dysfunction and supraventricular
  tachyarrhythmias. (Circulation. 2009;119:1576-1585.)
                    Key Words: sinoatrial node 䡲 pacing 䡲 arrhythmia 䡲 ion channels 䡲 electrophysiology

I  t is well-established that sinoatrial node (SAN) dysfunction
   is common in patients with atrial fibrillation (AF) and can
lead to syncopal attacks after AF termination, a condition
                                                                            the condition, Elvan et al5 demonstrated in an elegant study
                                                                            that electrically induced AF causes SAN dysfunction in dogs,
                                                                            with SAN abnormalities becoming reversed within a week
often called the tachycardia-bradycardia syndrome.1,2 Al-                   after AF termination. SAN dysfunction noted after termina-
though abnormalities of SAN structure have been noted in                    tion of chronic atrial flutter was also found to reverse itself
patients with AF,3 there is increasing evidence of a reversible             over several weeks,6 which supports the notion that atrial
component related to SAN remodeling caused by rapid atrial                  tachyarrhythmias lead to reversible SAN dysfunction in
tachyarrhythmias. SAN dysfunction is commonly noted 1 day                   humans. Subsequently, Hocini et al7 demonstrated that when
after electrical cardioversion in patients with lone AF.4                   AF patients show prolonged sinus pauses on AF termination,
Although this finding was originally believed to be due to the              successful AF ablation is followed by marked recovery in
intrinsic electrophysiological abnormalities that characterize              SAN function indices.

  Received April 30, 2008; accepted October 17, 2008.
  From the Department of Medicine (Y.-H.Y., B.B., X.Y.Q., M.S., D.C., P.C., Z.W., S.N.), Department of Physiology and Institute of Biomedical
Engineering (P.C.), Montreal Heart Institute Research Center and Université de Montréal, Montreal, Quebec, Canada; Department of Pharmacology and
Therapeutics (B.B., S.N.), McGill University, Montreal, Quebec, Canada; and the First Cardiovascular Division, Chang-Gung Memorial Hospital and
Chang-Gung University (Y.-H.Y., C.-T.K.) Tao-Yuan, Taiwan.
  *The first 3 authors contributed equally to this work.
  The online-only Data Supplement is available with this article at http://circ.ahajournals.org/cgi/content/full/CIRCULATIONAHA.108.789677/DC1.
  Correspondence to Stanley Nattel, 5000 Belanger St E, Montreal, H1T 1C8, Quebec, Canada. E-mail stanley.nattel@icm-mhi.org
  © 2009 American Heart Association, Inc.
  Circulation is available at http://circ.ahajournals.org                                         DOI: 10.1161/CIRCULATIONAHA.108.789677

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Funny Current Downregulation and Sinus Node Dysfunction Associated With Atrial Tachyarrhythmia
Yeh et al        SA Node Ion Channels in Tachy-Brady Syndrome                             1577

      Table.   Gene-Specific Primers (and TaqMan Probe Sequences) Used in Real-Time RT-PCR Analysis
      Gene             Forward Primer Sequence             Reverse Primer Sequence              Probe Sequence             Accession No.
      Cav1.2      GACATTGTTTTCACTACCATTTTCACCAT         GGCAAAAAGAGCCCTTATGTAGGAA            ATCTTCAGAGCAATTTC              XM_534932
      Cav3.1           GAGGACATCGCCTGTGACT               GGCAAAGAAGGCAAAGATGAAGTC             ATCCTGCAGGCCTTTG              XM_860802
      Cx43            ACTCTTGTACCTGGCTCATGTG               ACCTTGCCGTGCTCTTCAAT                                           NM_001002951
      ERG            GCAAAGTGGAGATCGCCTTCTAC               CATCCACCAGGCACAGGAA                CAGCTCCCATCCTTCC            NM_001003145
      HCN2               GCGGCGCCAGTACCA                  GCAGCTTGTGGAAGGACATGTA             CCTGCTTGTACTTCTCC              XM_850140
      HCN4              CTGGGCGAGCTGAGTGA                   CCAGCTTCCGGCAGTTGAA            CTGAGGGAGGAGATCATCAA             XM_535535
      Kv4.3          TCCCCTGTTATCTGTACGAACCT             TTCTGCTCAAACATCTGCTCATCT             CCACCATCAAGAACCA              XM_845974
      KvLQT1           ATTCGGCGCATGCAGTACTT                TTGATGCGCACCATGAGGTT                                             XM_540790
      minK          GGCTCTAGATCAGGAACCTTCTTG               CACGAAGGCCAAACATCACA               CCTGCAGCCGGTCACT              XM_544868
      MiRP1            CCATCCTGGTGAGCACTGT              AGTCCTCTACAATGTACTGGTGGTA             CCGTCTCTTGGATTTC              XM_544867

                                                                         and severing of the vagus nerves in the neck.2 The RA was then
               Clinical Perspective p 1585                               paced at cycle lengths (CLs) of 250 or 300 ms for 1 minute. The
   Despite extensive accumulating evidence for atrial                    corrected sinus node recovery time (SNRTc) was obtained from the
                                                                         interval from the last paced atrial activation to the first sinus escape
tachyarrhythmia–induced SAN dysfunction, the underlying                  beat, minus the prepacing spontaneous CL.
mechanisms have remained unclear. Atrial tachyarrhythmias,
including AF, cause substantial remodeling of the ionic current          RNA Extraction and TaqMan Real-Time
properties of atrial cardiomyocytes, which causes action poten-          Polymerase Chain Reaction
tial abbreviation that increases vulnerability to AF induction and       RNA was isolated from tissue samples by guanidine thiocyanate-
maintenance.8 It is quite conceivable that SAN ionic current             phenol-chloroform extraction, then treated with DNase (RNeasy
changes induced by AF lead to the depressed SAN function that            mini kit, Qiagen, Valencia, Calif), quantified, and subjected to
                                                                         quality control by microelectrophoresis on polyacrylamide gels
characterizes the tachycardia-bradycardia syndrome.                      (Agilent 2100 Bioanalyzer, Agilent Technologies Inc, Santa Clara,
   The present study tested the hypothesis that sustained atrial         Calif).1 DNA contamination was excluded by reverse-transcription–
tachycardia alters ionic current properties in SAN cardiomyo-            negative polymerase chain reaction (PCR). First-strand complemen-
cytes, thereby causing SAN dysfunction. We first studied                 tary DNA was synthesized from 2 ␮g of total RNA with a
changes in the expression of SAN ion channel subunits that               high-capacity cDNA archive kit (Applied Biosystems, Foster City,
                                                                         Calif). Real-time quantitative PCR was performed with either 6-car-
resulted from 1 week of atrial tachypacing (ATP) at 400 bpm,             boxy-fluorescein–labeled fluorogenic TaqMan primers and probes
a recognized paradigm of AF-related atrial tachycardia re-               (assay-by-design) with TaqMan universal master mix (Applied
modeling.8,9 We then developed the necessary methods to                  Biosystems) or custom primers (Invitrogen, Carlsbad, Calif) with
isolate canine SAN cardiomyocytes and performed voltage-                 SYBR Green master mix (Applied Biosystems; sequences provided
                                                                         in the Table). Fluorescence signals were detected with the Strat-
clamp studies to characterize the effects of ATP on their ionic          agene Mx3000P sequence-detection system (Stratagene, La Jolla,
currents. Our results implicate alterations of the funny cur-            Calif) in duplicate, normalized to the reference (18S ribosomal
rent, If, in atrial tachyarrhythmia–induced SAN dysfunction.             RNA, Applied Biosystems), and quantified with MxPro QPCR
                                                                         software (Stratagene).
                           Methods
                                                                         SAN Cardiomyocyte Isolation
Animal Handling and Tachypacing Protocol                                 An RA preparation containing the SAN region was perfused at ⬇10
Animal care procedures were consistent with National Institutes of       mL/min via the right coronary artery for cardiomyocyte isolation.9
Health guidelines and were approved by the animal research ethics        The preparation was first perfused with 2 mmol/L Ca2⫹-containing
committee of the Montreal Heart Institute. Adult mongrel dogs            Tyrode solution until all leaking coronary artery branches were
(weight 25 to 35 kg; Laka Inc, Saint-Basile-le-Grand, Quebec,            ligated, followed by Ca2⫹-free Tyrode solution for 15 minutes. Then,
Canada) were instrumented with a unipolar right-atrial (RA) lead         Ca2⫹-free Tyrode solution that contained collagenase (110 U/mL
attached to a pacemaker programmed to provide 1 week of RA               CLS II collagenase; Worthington Biochemical, Lakewood, NJ) and
pacing at 400 bpm. Ventricular rate control was ensured by radio-        0.1% bovine serum albumin was used to perfuse the preparation for
frequency ablation–induced AV block, with a right ventricular            ⬇40 minutes. The SAN region was identified as a whitish endocar-
demand pacemaker set at 80 bpm. ATP dogs (n⫽31) were compared            dial zone near the junction between the superior vena cava and the
with control dogs (n⫽28) that were similarly instrumented but with       RA appendage. Dispersed cells were stored in a high-K⫹ storage
the RA pacemaker inactivated. At the time the animals were               solution.
euthanized, SAN cardiomyocytes were isolated for electrophysiolog-
ical study, and SAN and RA free-wall (RAFW) tissue samples were          SAN Cellular Electrophysiology
collected, snap-frozen in liquid N2, and stored at ⫺80°C.                Currents were recorded with whole-cell patch clamping at 36⫾0.5°C
                                                                         as described previously.9 Potential SAN cardiomyocytes were iden-
Sinus Node Recovery Time Changes                                         tified on the basis of distinct morphologies (fine, elongated spindle-
On study days, dogs were anesthetized (morphine 2 mg/kg SC;              like or spider-shaped cells; see supplemental Figure I). Only cells
␣-chloralose 120 mg/kg IV load, 29.25 mg · kg⫺1 · h⫺1) and               presenting If, which was never seen in atrial cardiomyocytes, were
mechanically ventilated. Bipolar pacing and recording hook elec-         selected for SAN cell current recording. Borosilicate glass electrodes
trodes were inserted into the RA appendage. The baseline sinus rate      had tip resistances between 2.0 and 4.0 M⍀ when filled. Compen-
was measured after suppression of potentially confounding vagal and      sated series resistances and capacitive time constants averaged
␤-adrenergic influences by administration of nadolol (0.5 mg/kg IV)      3.4⫾0.3 M⍀ and 289⫾64 ␮s, respectively. SAN cell capacitance

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1578        Circulation         March 31, 2009

                                                                                                   Figure 1. In vivo recordings at baseline
                                                                                                   and immediately after overdrive pacing for
                                                                                                   sinus node recovery time (SNRT) mea-
                                                                                                   surements. A, Representative atrial ECG
                                                                                                   recordings from control (CTL) and ATP
                                                                                                   (AT-P) dogs. Left, Prepacing baseline AA
                                                                                                   recordings after vagotomy and intrave-
                                                                                                   nous nadolol. Right, Postpacing (at a CL
                                                                                                   of 250 ms) AA recordings. The SNRT was
                                                                                                   the first postpacing A-A interval. SNRTc
                                                                                                   was calculated as SNRT minus the mean
                                                                                                   prepacing AA CL. B, Mean⫾SEM sinus
                                                                                                   CL at baseline after vagotomy/intravenous
                                                                                                   nadolol. C, Mean⫾SEM SNRTc at 250
                                                                                                   and 300 ms pacing CLs. N⫽12 dogs per
                                                                                                   group. *P⬍0.05, ***P⬍0.01; analysis in B
                                                                                                   by unpaired Student t test, in C by
                                                                                                   repeated-measures 2-way ANOVA.

averaged 80⫾5 pF (n⫽30) for control dogs and 85⫾3 pF (n⫽44) for            each parameter value set were run for 100 seconds. The last 2
tachypaced dogs. Original recordings are presented in terms of             seconds of simulation were then analyzed to compare the effects of
absolute current amplitude, but mean data are shown as current             the observed degrees of If and IKs remodeling on SAN activity.
density (pA/pF). Junction potentials averaged 15.0⫾0.7 mV and were
corrected only for resting-potential measurements. Resting potentials      Statistical Analysis
averaged ⫺52⫾1 mV in control and ⫺53⫾1 mV in tachypaced SAN                Data are expressed as mean⫾SEM. Repeated-measures 2-way
cells (n⫽14 and 17 cells, respectively, from 3 dogs each).                 ANOVA and Bonferroni-adjusted t tests were used for statistical
                                                                           comparisons of current-voltage relations. Reverse-transcription PCR
Solutions                                                                  data (nonrepeated measures) were also analyzed by 2-way ANOVA.
The cell-storage solution contained (in mmol/L) KCl 20, KH2PO4 10,         When ANOVA revealed a statistically significant interaction,
dextrose 10, mannitol 40, L-glutamic acid 70, ␤-OH-butyric acid 10,        Bonferroni-adjusted comparisons were performed to compare indi-
taurine 20, EGTA 10, and 0.1% bovine serum albumin (pH 7.3,                vidual group means by multiplying each probability value by the
KOH). Tyrode (extracellular) solution contained (in mmol/L) NaCl           number of comparisons. In the absence of significant interactions,
136, KCl 5.4, MgCl2 1, NaH2PO4 0.33, HEPES 5, and dextrose 10              statistical data are presented only in terms of main effects (region
(pH 7.35, NaOH), with CaCl2 of 1 mmol/L for If recording and 2 or          [SAN versus RA] or condition [control versus ATP]). An unpaired
0 mmol/L for cell isolation. The internal solution for If and K⫹-current   Student t test was used to compare spontaneous CL between control
recording contained (in mmol/L) K-aspartate 110, KCl 20, MgCl2 1,          and ATP-remodeled dogs. Analyses of ionic currents controlled for
MgATP 5, Li-GTP 0.1, HEPES 10, Na-phosphocreatine 5, and                   dog of origin as a variable to avoid weighting results from different
EGTA 5 (pH 7.3, KOH). Ba2⫹ (1 mmol/L)-sensitive current was used           dogs by the number of cells studied. A 2-tailed probability value
to assess inward-rectifier K⫹ currents as described previously.9 For       ⬍0.05 was considered statistically significant. Clampfit 9.0 (Axon
IKs recording, nifedipine (5 ␮mol/L), 4-aminopyridine (2 mmol/L),          Instruments, Foster City, Calif) and GraphPad Prism 3.0 (GraphPad,
dofetilide (1 ␮mol/L), and atropine (200 nmol/L) were added to             San Diego, Calif) software were used for data analysis.
suppress ICaL, Ito, IKr, and 4-aminopyridine– dependent muscarinic K⫹         The authors had full access to and take full responsibility for the
currents. For IKr recording, the same solutions were used as for IKs,      integrity of the data. All authors have read and agree to the
except dofetilide was not included and the IKs blocker HMR 1556            manuscript as written.
(0.5 ␮mol/L) was added. The external solution for ICa recording
contained (in mmol/L) tetraethylammonium chloride 136, CsCl 5.4,                                        Results
CaCl2 2, MgCl2 0.8, HEPES 10, and dextrose 10 (pH 7.4, CsOH).
Niflumic acid (50 ␮mol/L) was added to inhibit Ca2⫹-dependent Cl⫺          SAN Recovery Times In Vivo
current . The internal solution for ICa recording contained (in mmol/L)    Figure 1A illustrates RA electrogram recordings used to
CsCl 120, TEA-Cl 20, MgCl2 1, MgATP 5, Li-GTP 0.1, EGTA 10, and            calculate the SNRTc. The left panel shows baseline after
HEPES 10 (pH 7.3, CsOH). Unless otherwise specified, chemicals were
obtained from Sigma Chemicals (St Louis, Mo).
                                                                           vagotomy and nadolol administration, whereas the right panel
                                                                           shows recordings just before and after the end of tachypacing.
Transmembrane Potential Simulations                                        There was a clear delay to the emergence of the first
The Kurata model of the rabbit SAN cell action potential10 was             spontaneous postpacing beat, which was enhanced after 1
modified to produce a spontaneous rate similar to that in dogs in the      week of ATP. After vagotomy and nadolol administration,
present study (the sustained inward current [Ist] was set to zero) and     the sinus CL was longer in ATP dogs than in control
implemented in C⫹⫹ on an AMD64 processor– based computer
(AMD, Sunnyvale, Calif). Model implementation used a variable              (P⬍0.05), which indicates reduced intrinsic SAN automatic-
time-step algorithm (Runge-Kutta-Merson fourth-order integration           ity (Figure 1B). SNRTc was substantially prolonged in ATP
scheme) with maximum relative tolerance of 10⫺6. Simulations with          dogs versus controls (Figure 1C).
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Yeh et al       SA Node Ion Channels in Tachy-Brady Syndrome                    1579

                                                                                 Figure 2. Real-time reverse-transcription PCR.
                                                                                 Mean⫾SEM mRNA expression of If-associated
                                                                                 subunits (A–C), IK-related subunits (D–F), ICaL and
                                                                                 ICaT subunits (G and H), and Cx43 (I). N⫽8 to 10
                                                                                 per group. *P⬍0.05, ***P⬍0.001 for individual
                                                                                 group mean differences by Bonferroni-adjusted t
                                                                                 test in the presence of significant group-by-region
                                                                                 interaction; ††P⬍0.01, †††P⬍0.001 for main effect
                                                                                 of region; ‡‡P⬍0.01 for main effect of condition;
                                                                                 by 2-way ANOVA. CTL indicates control;
                                                                                 AT-P, ATP.

Ion Channel Subunit Expression                                      IK Subunits
ATP causes atrial electrical remodeling, which alters the           Expression of the ␣-subunits corresponding to IKr (ERG;
mRNA expression of ion channel subunits.8 Quantitative              Figure 2D) and IKs (KvLQT1; Figure 2E) was similar for
reverse-transcription PCR was used to investigate ATP-              RAFW versus SAN tissues, and neither was significantly
induced changes in mRNA expression profiles and to com-             altered by ATP. The IKs ␤-subunit minK (Figure 2F) was
pare SAN and RAFW expression patterns to support tissue-            more strongly expressed in SAN than in RAFW (main effect
identification validity (Figure 2).                                 of region: F⫽12.42, dfn⫽1, dfd⫽33, P⫽0.0013) and was
                                                                    globally reduced by ATP (main effect of condition: F⫽11.48,
If Subunits                                                         dfn⫽1, dfd⫽33, P⫽0.0018).
The mRNA expression levels of hyperpolarization-activated
cyclic nucleotide– gated subunits (HCN2 and HCN4) were              ICa Subunits
enriched in control SAN tissue versus RAFW (⬇5.5-fold and           The L-type Ca2⫹ current (ICaL) ␣-subunit Cav1.2 (main effect
12-fold, respectively, P⬍0.001 for both; Figures 2A and 2B).        of region: F⫽22.95, dfn⫽1, dfd⫽32, P⬍0.0001; Figure 2G)
There was a statistically significant interaction between           and T-type Ca2⫹ current (ICaT) ␣-subunit Cav3.1 (main effect
region (RAFW and SAN) and condition (control versus ATP)            of region: F⫽10.25, dfn⫽1, dfd⫽33, P⫽0.0030; Figure 2H)
in expression of both HCN2 (F⫽5.67, dfn⫽1, dfd⫽34,                  were both more strongly expressed in SAN than in RAFW.
P⫽0.023) and HCN4 (F⫽6.07, dfn⫽1, dfd⫽34, P⫽0.019),                 Neither subunit was significantly affected by ATP.
which indicates that region is a determinant of the response to     Connexin43
ATP. ATP reduced SAN expression of both HCN2 (by 56%,               There was a significant interaction between region and
P⬍0.05) and HCN4 (59%, P⬍0.05). There was no interac-               condition for connexin 43 (Cx43) expression (F⫽26.21,
tion between region and condition for the putative If               dfn⫽1, dfd⫽32, P⬍0.0001; Figure 2I). In control tissue,
␤-subunit MiRP1 (Figure 2C), but there was a significant            Cx43 was expressed ⬇3.4-fold more in RAFW than in SAN
main effect of region (F⫽15.70, dfn⫽1, dfd⫽30, P⫽0.0004),           (P⬍0.001). Although ATP had no effect on SAN Cx43
with greater expression in SAN than in RAFW, which was              expression, tachypacing downregulated Cx43 by 48%
unchanged by tachypacing.                                           (P⬍0.001) in RAFW.
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1580       Circulation       March 31, 2009

Figure 3. Reduction of SAN hyperpolarization-activated current (If) by atrial tachycardia remodeling. A, Representative If recordings
from control (CTL; left) and ATP (AT-P; right) SAN cardiomyocytes. B, Mean⫾SEM If step-current density-voltage relations. C, Activa-
tion kinetics of step If. D, Voltage-dependent If activation. N⫽14 cells from 10 dogs for control and 16 cells from 10 dogs for ATP. TP
indicates test potential. *P⬍0.05, **P⬍0.01, ***P⬍0.001, by repeated-measures 2-way ANOVA.

Reference Gene                                                          3D). The V1/2 and slope-constant values obtained from Bolt-
Expression of the reference gene, which encoded 18S ribo-               zmann fits of data in each experiment averaged ⫺72.4⫾3.0
somal RNA, was comparable among groups (RAFW control                    and ⫺8.3⫾1.1 mV, respectively, for control cells and
6.38⫾0.47; RAFW ATP 6.75⫾0.63; SAN control                              ⫺76.4⫾3.3 and ⫺7.6⫾1.3 mV, respectively, for ATP cells.
6.68⫾0.67; SAN ATP 6.77⫾0.31).                                             We also recorded Ba2⫹-sensitive K⫹ currents in SAN cells
                                                                        to assess their constitutive acetylcholine-regulated (IKACh) and
Hyperpolarization-Activated Currents                                    background inward-rectifier (IKl) expression phenotype and to
Ionic currents were selected for measurement on the basis of            evaluate possible ATP-induced inward-rectifier current up-
the mRNA data. The 2 currents that showed significant                   regulation of the type previously observed in atrial cells.8
subunit expression changes (If and IKs) were recorded, along            Supplemental Figures IIA and IIB show such current record-
with 2 Ca2⫹ currents believed to play important roles in SAN            ings from 1 SAN cell before and after exposure to 1 mmol/L
pacemaking4 that showed no significant alteration in                    Ba2⫹. Consistent with very limited IKl expression in the SAN
␣-subunit expression (ICaL and ICaT) and 1 K⫹ current that              region, Ba2⫹ had no clear effect on the currents, and the
showed no mRNA change (IKr). Figure 3A shows represen-                  Ba2⫹-sensitive currents obtained by digital subtraction (sup-
tative recordings of If in control and ATP cells. Both                  plemental Figure IIC) were negligible. Mean Ba2⫹-sensitive
time-dependent activating (Figure 3B) and tail-current com-             current-voltage density relations in SAN cells are illustrated
ponents of If were significantly reduced by ATP. For exam-              in supplemental Figure IID and contrasted with correspond-
ple, at a step voltage of ⫺140 mV, If was reduced from                  ing results in RA cardiomyocytes. Unlike SAN cells, atrial
⫺10.8⫾1.0 pA/pF in control cells to ⫺6.0⫾1.0 pA/pF in                   cardiomyocytes showed clear Ba2⫹-sensitive currents with
ATP cells (P⬍0.001). If activation kinetics were well fitted            current-voltage relations typical of inward-rectifier K⫹ cur-
by biexponential relations and were not affected by ATP                 rents. These results support the characteristic ion channel
(Figure 3C), showing similar fast (␶fast) and slow (␶slow)              properties of the canine SAN cells that we studied, because
activation time constants, of the order of 50 to 200 and 250 to         lack of IKl is characteristic of most mammalian SAN cells.11
1000 ms, respectively, over the full voltage range. To analyze          In addition, we recorded Ba2⫹-sensitive currents in SAN cells
steady state activation voltage dependence, If tail currents on         isolated from ATP dogs. As shown by the results in supple-
repolarization to ⫺140 mV were normalized by the maxi-                  mental Figure IID, no significant Ba2⫹-sensitive currents were
mum tail-current value and plotted as a function of the                 detected, which indicates the absence of inward-rectifier
preceding step potential. Tachypacing did not significantly             current (IKl or constitutive IKACh) upregulation by ATP in SAN
affect the activation variable at different voltages (Figure            cells.
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Yeh et al        SA Node Ion Channels in Tachy-Brady Syndrome                        1581

                                                                                               Figure 4. Reduction of SAN slow
                                                                                               delayed-rectified K⫹ current (IKs) by atrial
                                                                                               tachycardia remodeling. A, Representative
                                                                                               IKs recordings from control (CTL; left) and
                                                                                               ATP (AT-P; right) SAN cardiomyocytes.
                                                                                               B and C, Mean⫾SEM IKs step- and tail-
                                                                                               current density-voltage relations. D,
                                                                                               Mean⫾SEM normalized IKs tail-current
                                                                                               density-voltage relation. E, Activation
                                                                                               kinetics of step IKs and deactivation kinet-
                                                                                               ics of tail IKs. N⫽15 cells from 10 dogs
                                                                                               per group. *P⬍0.05, **P⬍0.01, ***P⬍0.001,
                                                                                               by repeated-measures 2-way ANOVA.

Delayed-Rectifier Currents                                              step-current activation and tail-current deactivation showed
The slow delayed-rectifier current IKs plays important roles in         no significant differences between control and ATP values, as
SAN pacemaking in most species,11 and IKs ␤-subunit minK                illustrated in Figure 4E.
gene expression was downregulated. Figure 4A shows re-                     Results of rapid delayed-rectifier current recordings are
cordings of IKs in control and ATP cells. Both the step (Figure         shown in Figure 5. IKr tail currents were recorded during
4B) and tail (Figure 4C) currents were significantly reduced            4-second repolarizing pulses to ⫺40 mV after a 2-second
by ATP. For example, the IKs step-current density at 60 mV              activating pulse to voltages between ⫺40 and 70 mV. As
was reduced from 12.3⫾0.7 pA/pF in control to 8.8⫾0.7                   shown in Figures 5A and 5B, IKr tail currents were small in
pA/pF in ATP cells (P⬍0.001), whereas the tail current density          canine SAN cells, both from dogs without and with ATP.
was reduced from 2.9⫾0.3 pA/pF in control to 1.9⫾0.2 pA/pF              Figure 5C shows mean tail-current density-voltage relations,
in ATP (P⬍0.01). Voltage dependence of IKs activation (tail-            which were unchanged by ATP.
current analysis) was not altered by tachypacing (Figure 4D),
with control and ATP cells showing similar mean V1/2 values,            Calcium Currents
which averaged 10.2⫾2.1 and 13.5⫾2.4 mV, respectively.                  Ca2⫹ currents are important in SAN pacemaking,11 and atrial
The time courses of both step-current activation on depolar-            tachyarrhythmias have been shown to change atrial ICaL, with
ization to 60 mV and tail-current deactivation on repolariza-           both transcriptional and posttranscriptional mechanisms hav-
tion from 60 to ⫺40 mV were biexponential. Time constants               ing been implicated.8 Accordingly, we compared ICaL and ICaT
corresponding to both the slow and fast components of                   in SAN cells from control and ATP dogs. Original ICaL

Figure 5. Unchanged SAN rapid delayed-rectifier K⫹ current (IKr) in atrial tachycardia remodeling. A and B, IKr recordings from control
(CTL; A) and ATP (AT-P; B) SAN cardiomyocytes. C, Mean⫾SEM IKr tail-current density-voltage relations from 14 cells from 3 control
dogs and 26 cells from 5 ATP dogs. Analysis by repeated-measures 2-way ANOVA. TP indicates test potential.

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1582      Circulation        March 31, 2009

Figure 6. Unaltered SAN calcium currents (ICaL and ICaT) with atrial tachycardia remodeling. A, Representative recordings of ICaL from
control (CTL; left) and ATP (AT-P; right) SAN cardiomyocytes. B, Mean⫾SEM ICaL density-voltage relations; n⫽12 cells from 4 dogs per
group. C, Representative recordings of calcium current from control and ATP cardiomyocytes, respectively. The currents were recorded
with holding potentials of ⫺90 mV and ⫺50 mV. The subtracted currents represent ICaT. D, Mean⫾SEM ICaT density-voltage relations;
n⫽10 cells from 4 dogs per group. Analysis by repeated-measures 2-way ANOVA. TP indicates test potential.

recordings are shown in Figure 6A. ICaL densities were                absence of autonomic influences (vagotomy/nadolol). Simu-
comparable between ATP and control cells (Figure 6B). For             lation of the decrease in If and IKs together did not appreciably
example, at 10 mV, ICaL density averaged ⫺4.6⫾0.6 pA/pF in            alter the slowing effect of simulated ATP compared with If
control and ⫺4.1⫾0.7 pA/pF in ATP cells, respectively.                effects alone (CL 443.6 ms with If reduction alone versus
Original recordings corresponding to total ICa (including both        443.4 ms with combined IKs/If reduction).
ICaL and ICaT components) obtained on depolarization from
⫺90 to ⫺20 mV and recordings from the same cells that                                          Discussion
reflect ICaL without a contribution from ICaT (obtained by            We have completed a detailed analysis of the functional and
depolarization from ⫺50 to ⫺20 mV) are shown in Figure                gene expression changes for selected ion channel subunits of
6C. T-type current was obtained by subtracting currents               SAN cells isolated from dogs subjected to 1 week of atrial
recorded with a holding potential of ⫺50 mV from current              tachycardia remodeling. The results indicate significant
recorded with a holding potential of ⫺90 mV, as described             changes in the expression of specific subunits involved in
previously.9 ICaT was not present in all SAN cells but was            SAN pacemaking, with alterations in If appearing to be
found in a large and similar proportion (⬇70%) of both                particularly important for associated SAN dysfunction.
control and ATP cells. No significant change in ICaT (Figure
6D) current density-voltage relations was produced by ATP;            Mechanisms Underlying Reversible SAN Dysfunction
for example, ICaT at ⫺20 mV was ⫺1.4⫾0.4 for control                  in Tachycardia-Bradycardia Syndrome
versus ⫺1.3⫾0.2 pA/pF for ATP cells.                                  Early studies implicated anatomic structural abnormalities in
                                                                      SAN dysfunction associated with atrial tachyarrhythmias,
Transmembrane Potential Simulations                                   which suggests a fixed SAN dysfunction substrate1,3; how-
Simulation of SAN cell action potentials provided the spon-           ever, several subsequent lines of evidence have pointed to an
taneous activity shown by the blue curves in Figure 7. The            important functional, and potentially reversible, component.
results of different combinations of If and IKs remodeling are        Elvan et al5 showed that electrically sustained AF over 2 to 6
superimposed in specific colors. Remodeling of IKs alone was          weeks induced SAN dysfunction in parallel with atrial re-
simulated by reproducing the same mean density decrease               modeling and that significant SAN recovery occurred within
(35%) obtained in voltage-clamp recordings and did not                1 week of AF cessation. These observations were confirmed
change spontaneous SAN cell periodicity (CL 407.8 ms in               by studies that showed that SAN dysfunction due to atrial
control versus 407.7 ms with reduced IKs). A 50% decrease in          tachycardia remodeling was fully reversed 4 weeks after
If slowed periodic activity by increasing the CL ⬇9%, which           tachycardia cessation.12 Termination of chronic atrial flutter
was a change of the same order as but slightly less than the          in humans is followed by progressive improvement in SNRTc
increase in spontaneous CL seen with ATP (13.8%) in the               abnormalities over 3 weeks, which supports the applicability
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Yeh et al       SA Node Ion Channels in Tachy-Brady Syndrome                     1583

                                                                                          Figure 7. Simulations of SAN transmem-
                                                                                          brane potential showing changes in spon-
                                                                                          taneous periodicity caused by reductions
                                                                                          in If, IKs, and If and IKs together. V indi-
                                                                                          cates voltage; t, time.

of the experimental findings to clinical tachyarrhythmias.6         density. The tachycardia-induced downregulation of ICaL and
Paroxysmal AF patients with prolonged (⬎3 seconds) sinus            upregulation of inward-rectifier K⫹ currents that are believed
pauses on AF termination show progressive improvements in           to be of great functional importance at the atrial level8 are not
sinus node function after AF ablation, with a clinical evolu-       observed in SAN cells.
tion that indicates an absence of clinically significant SAN           mRNA profiling in the present study showed some inter-
disease.7 The results obtained in the present study provide a       esting differences between ATP-induced remodeling in SAN
potential ionic current mechanism to explain these experi-          and RAFW. HCN subunits were downregulated only in SAN
mental and clinical observations, based on atrial                   tissue, and Cx43 was downregulated only in RAFW. The IKs
tachycardia–induced remodeling of SAN ion channel expres-           ␤-subunit minK was downregulated in both SAN and RAFW.
sion and function. If contributes to cellular automaticity by       Although we found SAN IKs to be downregulated by ATP in
depolarizing cells toward their threshold potential, whereas        the present study, previous reports have not described corre-
IKs can contribute by accelerating phase 3 repolarization and       sponding changes in atrial tissue.9 Cav1.2 mRNA expression
advancing the time when the cell begins spontaneous phase 4         was not altered by ATP in either SAN or RAFW. The SAN
depolarization. Our mathematical modeling analyses suggest          result is consistent with unchanged SAN ICaL in the present
that the If changes caused by atrial tachycardia remodeling         study, but the atrial findings are discrepant with results of
largely account for the associated SAN dysfunction. The lack        previous investigations of ATP-induced atrial remodeling.8
of a significant role for IKs changes is likely due to the very     The reason for this discrepancy in atrial Cav1.2 mRNA
positive activation potential for this current,9 which is not       changes is unclear and may relate to technical factors or the
attained by SAN cells with their low resting potential and          site of atrial sampling, but a detailed experimental analysis
limited overshoot.11                                                goes beyond the scope of the present study.
                                                                       The basis for the differential atrial tachycardia remodeling
Relationship to Previous Studies of Atrial                          response of atrial cardiomyocyte ionic currents versus those
Tachycardia Remodeling and Disease-Related                          in SAN cells is unclear. Although we were unable to identify
SAN Dysfunction                                                     previous studies of SAN cell ion-channel remodeling with atrial
Atrial ionic current remodeling due to sustained atrial             tachycardia, Verkerk et al19 have analyzed in detail the
tachycardia has been evaluated in detail. The principal             changes in SAN ionic currents caused by congestive heart
changes include downregulation of ICaL8,9,13,14 and Ito8,9,14,15    failure in rabbits with chronic pressure and volume overload.
and upregulation of inward-rectifier K⫹ currents.8,14 –18 Atrial    They found changes quite similar to those we noted here, with
delayed-rectifier K⫹ current function is not altered by atrial      decreases in If and IKs and lack of change in the other currents
tachycardia.8,9 Changes in atrial If function have not been         they studied. The alterations that they observed were of the
described in atrial tachycardia remodeling. The profile of          same order that we saw but were slightly smaller: ⬇40%
atrial tachycardia–induced SAN remodeling differs substan-          versus ⬇50% decrease in If and ⬇20% versus ⬇35% de-
tially from changes seen at the atrial level, being dominated       crease in IKs. They also performed mathematical modeling to
by alterations in HCN subunits and If function, along with          assess the relative importance of If and IKs changes to altered
statistically significant changes in minK expression and IKs        SAN automaticity, and like us, they concluded that If down-
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1584      Circulation        March 31, 2009

regulation is the principal contributor. Verkerk et al19 did not    for study on the basis of SAN localization in the dog that we
examine the molecular basis of the SAN ionic current                identified in previous studies,20 and we used well-described
remodeling they observed with congestive heart failure, but         morphological criteria24,25 to define SAN-derived spider and
we subsequently studied HCN subunit expression changes in           spindle cells. The SAN preparations that we used for real-
a canine ventricular-tachypaced congestive heart failure            time PCR quantification of ion channel subunits also had
model and observed downregulation of HCN2 and HCN4                  subunit distribution properties typical of SAN: greater mRNA
mRNA.20 The similarities in SAN ionic current changes that          expression-levels of HCN2, HCN4, and MiRP1 subunits and
occur with congestive heart failure–induced and ATP-related         lower expression levels of Cx43 than RA tissue.20,26
remodeling are striking and may suggest a characteristic SAN           Although the present results are compelling evidence for a
ionic current response to pathological insults. A possible          contribution of HCN/If downregulation to ATP-induced SAN
explanation for the lack of changes in SAN ICaL and inward-         dysfunction, we cannot exclude the possibility that other
rectifier K⫹ currents could be that SAN cells are not fired at      changes may contribute as well. We did not study the
such high frequencies as atrial cardiomyocytes during AF,           properties of all channels, ion transporters, and ion-handling
owing to SAN slow-channel properties that cause entry block         systems in SAN tissue. In particular, there is recent evidence
into the central SAN and limit follow frequencies.21                for an important contribution of sarcoplasmic reticulum Ca2⫹
                                                                    uptake and release processes to cardiac pacemaking.27 Thus,
Novelty and Potential Significance                                  changes in important components of the cellular Ca2⫹-
The present study is the first of which we are aware to study       handling machinery, including, for example, the Na⫹,Ca2⫹
changes in SAN ion channel subunit expression and ionic             exchanger, sarcoplasmic reticulum Ca2⫹ ATPase (SERCA),
current function with atrial tachycardia remodeling. Our            the ryanodine receptor, calsequestrin, and phospholamban,
results provide novel insights into the fundamental mecha-          could have been changed by ATP and could contribute to
nisms at the ionic and molecular level responsible for a            altered SAN automaticity. A role for other ionic currents
clinically important phenomenon, the SAN dysfunction that           cannot be excluded, including Cl⫺ currents such as the
is associated with atrial tachyarrhythmias. The importance of       Ca2⫹-dependent, swelling-induced, and cAMP-regulated Cl⫺
this problem has been underlined in a recent detailed review        current; K⫹ currents such as the Ca2⫹-dependent K⫹ current;
of SAN physiology in relation to sick sinus syndrome, with          various 2-pore, 4-transmembrane domain channels; and non-
an absence of information about the underlying molecular/           selective cation channels. In addition, adrenergic and cholin-
ionic basis being evident.22 The observation that HCN subunit       ergic regulation importantly modify If function and SAN
downregulation underlies SAN dysfunction in the present             automaticity. We cannot exclude a role for ATP-related
experimental model of tachycardia-bradycardia syndrome, as          changes in autonomic and associated G-protein– coupled
it does in experimental congestive heart failure,19,20 provides     regulation of If or other currents controlling SAN function.
further rationale for the development of cell/gene therapy          Nevertheless, we have succeeded in identifying congruent
approaches that involve HCN subunit expression enhance-             ionic current and channel subunit mRNA changes that are
ment for the management of clinical bradyarrhythmia                 consistent with previous studies of SAN pathological remod-
syndromes.23                                                        eling and that on the basis of an ion-current– based SAN
   The present study is also the first to the best of our           mathematical model account for a substantial portion of the
knowledge to study the properties of ionic currents in the          SAN slowing that we observed. Finally, although the present
canine SAN. The rabbit has been the species most commonly           results implicate HCN/If remodeling in ATP-induced SAN
used for SAN cell isolation and study, but the dog has clear        dysfunction, we did not study the underlying molecular
advantages in terms of widespread availability of clinically        mechanisms, which would be an appropriate objective for
relevant pathological models. Kwong et al24 isolated cells of       future studies.
various morphologies from canine SAN preparations and
described spider- and spindle-shaped cells as having unique                               Acknowledgments
connexin distribution properties that suggested a primary role      The authors thank Nathalie L’Heureux and Chantal St-Cyr for
in pacemaking function. The same group subsequently iso-            technical assistance and France Thériault for secretarial support.
lated cells with these morphologies from rabbit SANs and
showed that they have prominent If-like currents, which are                              Sources of Funding
                                                                    This study was supported by the Canadian Institutes of Health Research
larger for the spider-type cells.25 In the present study, we
                                                                    (Award MOP 44365), the Quebec Heart and Stroke Foundation, the
confirmed the prominent If shown by these cell types in             Mathematics of Information Technology and Complex Systems (MI-
canine SAN, which contrasts with the lack of If that we noted       TACS) Network of Centers of Excellence, and the European-North
in atrial cells. The present studies thus provide further           American Atrial Fibrillation Research Alliance (ENAFRA) network
evidence for the pacemaker-cell phenotype specialization of         award from Fondation Leducq. Dr Burstein received a Canadian
                                                                    Institutes of Health Research (CIHR) MD/PhD studentship.
spider and spindle cells in the dog. Further studies of SAN
cell pathophysiology in other canine models of human car-
                                                                                               Disclosures
diac disease would be of potential interest.                        None.

Potential Limitations                                                                           References
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Yeh et al            SA Node Ion Channels in Tachy-Brady Syndrome                                1585

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                                                               CLINICAL PERSPECTIVE
    Sinoatrial node dysfunction is frequently associated with atrial tachyarrhythmias, and patients with the combination are said
    to suffer from the relatively common tachycardia-bradycardia syndrome. Abnormalities in sinus node pacemaker function
    on termination of atrial tachyarrhythmias such as atrial fibrillation can cause syncope and require pacemaker implantation,
    but the underlying mechanisms remain poorly understood. There is evidence from clinical and experimental studies that
    suggests that a significant component of sinus node dysfunction in patients with the tachycardia-bradycardia syndrome may
    actually be caused by supraventricular tachyarrhythmia and may be reversible if the tachyarrhythmia is controlled. The
    present study examined the hypothesis that very rapid atrial tachyarrhythmias can cause ion channel downregulation in the
    sinus node, thereby causing abnormal sinus node function. Dogs subjected to atrial tachypacing at 400 bpm for 7 days
    showed prolonged sinus node recovery time, which indicates sinus node dysfunction. Ion channel subunit messenger RNA
    expression was measured in sinus node tissue and showed downregulation by atrial tachycardia of 2 specific types of
    subunits: Those underlying the funny current, which is known to be particularly important in cardiac pacemaking activity,
    and an accessory subunit involved in the slow delayed-rectifier K⫹ channel. Patch-clamp studies on sinus node cells
    isolated from control dogs and dogs subjected to atrial tachypacing confirmed the specific downregulation of funny current
    and slow delayed-rectifier K⫹ current with atrial tachycardia. These alterations were incorporated in a mathematical model
    of sinus node electrical activity, which suggested that the funny current changes were the principal factor in sinus node
    suppression by atrial tachycardia. Our results provide insights into the molecular mechanisms underlying clinically
    significant bradycardic complications of this common and important clinical syndrome.

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Online Figure I.
                            Supplemental Material

    A.                                         B.

         SAN

                                                      SAN
                      m

                            40 μm                   40 μm

Representative spindle-like (A) and spider-like (B) SAN cells. SAN cells are labelled
“SAN”. An atrial cardiomyocyte is seen for reference in panel A and is labelled “m”.
Online Figure II.
 A.                                                            B.
                          Baseline                                                                                     After 1 mM barium

                                                2 sec
                                      -40 mV

                                                                   500 pA
                                                         -140 mV

                                                                            500 ms

                                                                                                                     Atrium (n=10 cells/3 dogs)
 C.         Barium-sensitive current                               D.                                                SAN (CTL,n=9 cells/3 dogs)
                                                                                                                     SAN (ATP, n=13 cells/4 dogs)
                                                                                                               2

                                                                                     Current density (pA/pF)
                 200 pA

                                                                                                               0
                                                                                                                    -125   -100   -75   -50   -25   0
                          500 ms                                                                               -2

                                                                                                               -4

                                                                                                               -6

                                                                                                               -8

Hyperpolarization-activated currents in SAN cells were Ba2+-insensitive. Recordings from an SAN cell are shown before (A)
and after (B) Ba2+ superfusion (1 mmol/L). Digitally-subtracted Ba2+-sensitive currents are shown in C, and mean current-
  voltage relations for the Ba2+-sensitive currents in SAN-cells are contrasted with currents in atrial cardiomyocytes in D.
     Studies were also performed with SAN-cells isolated from AT-P dogs, with mean data indicated by the triangles.
Funny Current Downregulation and Sinus Node Dysfunction Associated With Atrial
    Tachyarrhythmia: A Molecular Basis for Tachycardia-Bradycardia Syndrome
Yung-Hsin Yeh, Brett Burstein, Xiao Yan Qi, Masao Sakabe, Denis Chartier, Philippe Comtois,
                      Zhiguo Wang, Chi-Tai Kuo and Stanley Nattel

         Circulation. 2009;119:1576-1585; originally published online March 16, 2009;
                        doi: 10.1161/CIRCULATIONAHA.108.789677
    Circulation is published by the American Heart Association, 7272 Greenville Avenue, Dallas, TX 75231
                     Copyright © 2009 American Heart Association, Inc. All rights reserved.
                                Print ISSN: 0009-7322. Online ISSN: 1524-4539

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